FvH4_7g23071

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001926
Physical Location & Seq
Forward (+)
18080155 .. 18083673
3519 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23071.t2

Sequence Viewer

Length: 1098 bp
ATGAGAGCTATGAAACAGAGTGGTGGATGGAAAAGGTTTCAATTTATACCCACTGCCAGAGTTCCCCTTTTAAAGGTCGAAAGTTATCTCCAAAATATCTCTTGTGATATATCAATCAATAATCTGAAGGGACAAATGAAATCCAAACTCTTGTTTTGTATTGGTGAGATAGATGGGCGCTTCCGTGATATGGTTTTACTGGTAAAGGAATGGGCAAAATCACAACATATGAATAATCCGAGATTGGGGTCTTTCAATTCATACGCCCTCACTTTGCTTGTGGTCTTCCACTTTCAGACATGTACACCTGCAATTTTGCCACCTCTTAAAGATATATATCCAGGGAATCTCACGGATGATCTTCAAGGCTTGAGGGCAGATGCAGAAAAACGTATTGAAGAGACATGTGTTGCTAACGTAACAAGGTTTAAATCAGACAAATTTGGAGCAGTAAACACAAGTTCATTGTCACAGCTTTTCATTTCATTTCTTGAGAAGTTTTCGGACCTTGTTTTGAAAACAAGAAAGTCAGGGATTTGCCCATATACCGGACAGTGGGAAGACATAGCAAGCAATATGACATGGTTGCCCCAAACATATACCATAATGATTGAAGATCCCTTTGATCAGCCAGAAAACTGTTCAAGGGCTGTTAGCATGGGAGAATTGACAAAGATATCTGAAGCTTGTAAGATGAGCAGCCACATACTCATGTCAGCTAATCAGAGTGACATTCTTGCCACACTAGTCCGACCCCTAACCTACCGTCTAGTGGCAGGAACACCTCACTACCCGTTTGAGCATCACCACACAGCTTATCCACAACGTTTCATGACTGAGAGTCCACATTCAAGAACACCTAGGGGCAATTATGCCAGGCAACCACATCGTCCTCAGGTGCATAAATGGGCACAGAAACCCTCACAAGTGCAGCCTCACTTTCAGAAGATGGAGCCAGAAAAACATCCTGACAAGTCCACCTTTCAAGAGATGAAGCCAGAAAACCATCCCAACAGGTCCACCAATGAAAACTCAACTCTAGAGACATCTCAGGACTGTGCATGCGGAATGTGTATGCTTGGGGAGTACATTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

366

Amino Acids

41.74

Weight (kDa)

8.71

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 3 - 54 2.6e-08 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 316
Acc36I ACCTGC 1 cut(s) 316
AciI CCGC 1 cut(s) 1065
AclI AACGTT 1 cut(s) 826
AclWI GGATC 1 cut(s) 611
AcsI RAATTY 1 cut(s) 440
AcuI CTGAAG 2 cut(s) 146, 702
AfaI GTAC 2 cut(s) 304, 1088
AfiI CCNNNNNNNGG 6 cut(s) 73, 190, 245, 548, 555, 772
AflIII ACRYGT 2 cut(s) 299, 404
AgsI TTSAA 9 cut(s) 41, 256, 365, 398, 517, 614, 645, 852, 986
AhdI GACNNNNNGTC 1 cut(s) 840
AhlI ACTAGT 1 cut(s) 745
AjnI CCWGG 2 cut(s) 340, 875
AluBI AGCT 5 cut(s) 8, 475, 686, 719, 815
AluI AGCT 5 cut(s) 8, 475, 686, 719, 815
Alw26I GTCTC 2 cut(s) 395, 1037
AlwI GGATC 1 cut(s) 611
ApeKI GCWGC 2 cut(s) 699, 931
ApoI RAATTY 1 cut(s) 440
AspA2I CCTAGG 1 cut(s) 860
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 2 cut(s) 505, 1017
AsuHPI GGTGA 2 cut(s) 176, 797
AvaII GGWCC 2 cut(s) 505, 1017
AvrII CCTAGG 1 cut(s) 860
AxyI CCTNAGG 1 cut(s) 894
BaeGI GKGCMC 1 cut(s) 913
BbsI GAAGAC 2 cut(s) 277, 567
BbvI GCAGC 2 cut(s) 711, 943
BccI CCATC 4 cut(s) 21, 167, 943, 1014
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BciT130I CCWGG 2 cut(s) 342, 877
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 395, 1037
BcuI ACTAGT 1 cut(s) 745
BfaI CTAG 4 cut(s) 746, 770, 861, 1040
BfoI RGCGCY 1 cut(s) 181
BfuAI ACCTGC 1 cut(s) 316
BisI GCNGC 2 cut(s) 700, 932
BlnI CCTAGG 1 cut(s) 860
BlsI GCNGC 2 cut(s) 701, 933
Bme1390I CCNGG 2 cut(s) 342, 877
Bme18I GGWCC 2 cut(s) 505, 1017
BmeRI GACNNNNNGTC 1 cut(s) 840
BmgT120I GGNCC 2 cut(s) 505, 1017
BmiI GGNNCC 1 cut(s) 954
BmrFI CCNGG 2 cut(s) 342, 877
BmsI GCATC 2 cut(s) 370, 811
BpiI GAAGAC 2 cut(s) 277, 567
BpuEI CTTGAG 2 cut(s) 391, 512
BsaBI GATNNNNATC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 341, 860
BsaWI WCCGGW 1 cut(s) 548
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 190, 245, 548, 555, 772
Bse1I ACTGG 1 cut(s) 204
Bse21I CCTNAGG 1 cut(s) 894
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 2 cut(s) 342, 877
BseDI CCNNGG 2 cut(s) 341, 860
BseGI GGATG 4 cut(s) 32, 361, 964, 1006
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 6 cut(s) 73, 190, 245, 548, 555, 772
BseMII CTCAG 3 cut(s) 828, 908, 1064
BseNI ACTGG 1 cut(s) 204
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 2 cut(s) 711, 943
BsgI GTGCAG 1 cut(s) 950
BsiSI CCGG 1 cut(s) 549
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 6 cut(s) 73, 190, 245, 548, 555, 772
BsmAI GTCTC 2 cut(s) 395, 1037
BsmFI GGGAC 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 913
Bsp1407I TGTACA 1 cut(s) 302
Bsp143I GATC 3 cut(s) 358, 616, 625
BspACI CCGC 1 cut(s) 1065
BspCNI CTCAG 3 cut(s) 829, 907, 1063
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 1 cut(s) 954
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 1 cut(s) 611
BsrGI TGTACA 1 cut(s) 302
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 341, 860
BssMI GATC 3 cut(s) 358, 616, 625
BssT1I CCWWGG 1 cut(s) 860
Bst2UI CCWGG 2 cut(s) 342, 877
Bst4CI ACNGT 4 cut(s) 555, 641, 767, 1058
Bst6I CTCTTC 1 cut(s) 393
BstAUI TGTACA 1 cut(s) 302
BstC8I GCNNGC 2 cut(s) 571, 1063
BstDEI CTNAG 3 cut(s) 837, 894, 1050
BstENI CCTNNNNNAGG 1 cut(s) 71
BstF5I GGATG 4 cut(s) 32, 361, 964, 1006
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 3 cut(s) 361, 619, 628
BstMAI GTCTC 2 cut(s) 395, 1037
BstMBI GATC 3 cut(s) 358, 616, 625
BstNI CCWGG 2 cut(s) 342, 877
BstNSI RCATGY 3 cut(s) 303, 408, 1065
BstSCI CCNGG 2 cut(s) 340, 875
BstSLI GKGCMC 1 cut(s) 913
BstV1I GCAGC 2 cut(s) 711, 943
BstV2I GAAGAC 2 cut(s) 277, 567
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
Bsu36I CCTNAGG 1 cut(s) 894
BtsCI GGATG 4 cut(s) 32, 361, 964, 1006
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 2 cut(s) 51, 560
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 2 cut(s) 571, 1063
CciI TCATGA 1 cut(s) 831
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 2 cut(s) 505, 1017
Csp6I GTAC 2 cut(s) 303, 1087
CviAII CATG 7 cut(s) 300, 405, 582, 658, 712, 832, 1062
CviQI GTAC 2 cut(s) 303, 1087
DdeI CTNAG 3 cut(s) 837, 894, 1050
DpnI GATC 3 cut(s) 360, 618, 627
DpnII GATC 3 cut(s) 358, 616, 625
DraI TTTAAA 2 cut(s) 72, 430
DriI GACNNNNNGTC 1 cut(s) 840
Eam1104I CTCTTC 1 cut(s) 393
Eam1105I GACNNNNNGTC 1 cut(s) 840
EarI CTCTTC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 860
Eco32I GATATC 1 cut(s) 678
Eco47I GGWCC 2 cut(s) 505, 1017
Eco57I CTGAAG 2 cut(s) 146, 702
Eco81I CCTNAGG 1 cut(s) 894
EcoNI CCTNNNNNAGG 1 cut(s) 71
EcoRII CCWGG 2 cut(s) 340, 875
EcoRV GATATC 1 cut(s) 678
EcoT14I CCWWGG 1 cut(s) 860
ErhI CCWWGG 1 cut(s) 860
FaeI CATG 7 cut(s) 303, 408, 585, 661, 715, 835, 1065
FalI AAGNNNNNCTT 2 cut(s) 965, 997
FaqI GGGAC 1 cut(s) 144
FatI CATG 7 cut(s) 299, 404, 581, 657, 711, 831, 1061
FauNDI CATATG 1 cut(s) 228
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 2 cut(s) 700, 932
FokI GGATG 4 cut(s) 39, 368, 951, 993
Fsp4HI GCNGC 2 cut(s) 700, 932
FspBI CTAG 4 cut(s) 746, 770, 861, 1040
GlaI GCGC 1 cut(s) 179
GluI GCNGC 2 cut(s) 700, 932
HaeII RGCGCY 1 cut(s) 181
HapII CCGG 1 cut(s) 549
HhaI GCGC 1 cut(s) 180
Hin1II CATG 7 cut(s) 303, 408, 585, 661, 715, 835, 1065
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 684
HinfI GANTC 2 cut(s) 346, 841
HpaII CCGG 1 cut(s) 549
HphI GGTGA 2 cut(s) 176, 797
Hpy166II GTNNAC 5 cut(s) 305, 454, 845, 978, 1020
Hpy188I TCNGA 9 cut(s) 126, 240, 297, 436, 505, 682, 726, 752, 945
Hpy188III TCNNGA 7 cut(s) 491, 832, 852, 968, 986, 1040, 1052
Hpy8I GTNNAC 5 cut(s) 305, 454, 845, 978, 1020
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 4 cut(s) 555, 641, 767, 1058
HpyCH4IV ACGT 3 cut(s) 391, 417, 826
HpyCH4V TGCA 5 cut(s) 311, 383, 901, 931, 1061
HpyF3I CTNAG 3 cut(s) 837, 894, 1050
HpySE526I ACGT 3 cut(s) 391, 417, 826
Hsp92II CATG 7 cut(s) 303, 408, 585, 661, 715, 835, 1065
HspAI GCGC 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 3 cut(s) 358, 616, 625
LmnI GCTCC 2 cut(s) 446, 952
Lsp1109I GCAGC 2 cut(s) 711, 943
LweI GCATC 2 cut(s) 370, 811
MaeI CTAG 4 cut(s) 746, 770, 861, 1040
MaeII ACGT 3 cut(s) 391, 417, 826
MaeIII GTNAC 3 cut(s) 418, 468, 728
MalI GATC 3 cut(s) 360, 618, 627
MboI GATC 3 cut(s) 358, 616, 625
MboII GAAGA 6 cut(s) 277, 353, 410, 572, 626, 958
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 1 cut(s) 913
MluCI AATT 6 cut(s) 41, 256, 312, 440, 665, 868
MlyI GAGTC 1 cut(s) 850
MmeI TCCRAC 1 cut(s) 775
MnlI CCTC 7 cut(s) 278, 333, 366, 795, 903, 931, 945
MseI TTAA 3 cut(s) 71, 327, 429
MslI CAYNNNNRTG 1 cut(s) 710
MspI CCGG 1 cut(s) 549
MspR9I CCNGG 2 cut(s) 342, 877
MvaI CCWGG 2 cut(s) 342, 877
NdeI CATATG 1 cut(s) 228
NdeII GATC 3 cut(s) 358, 616, 625
NlaIII CATG 7 cut(s) 303, 408, 585, 661, 715, 835, 1065
NlaIV GGNNCC 1 cut(s) 954
NmuCI GTSAC 2 cut(s) 468, 728
NspI RCATGY 3 cut(s) 303, 408, 1065
PaeI GCATGC 1 cut(s) 1065
PagI TCATGA 1 cut(s) 831
PaqCI CACCTGC 1 cut(s) 316
PciI ACATGT 2 cut(s) 299, 404
PfeI GAWTC 1 cut(s) 346
PkrI GCNGC 2 cut(s) 701, 933
PleI GAGTC 1 cut(s) 849
PpsI GAGTC 1 cut(s) 849
PscI ACATGT 2 cut(s) 299, 404
Psp1406I AACGTT 1 cut(s) 826
Psp6I CCWGG 2 cut(s) 340, 875
PspGI CCWGG 2 cut(s) 340, 875
PspN4I GGNNCC 1 cut(s) 954
PspPI GGNCC 2 cut(s) 505, 1017
PsuI RGATCY 1 cut(s) 616
RsaI GTAC 2 cut(s) 304, 1088
RsaNI GTAC 2 cut(s) 303, 1087
RseI CAYNNNNRTG 1 cut(s) 710
SaqAI TTAA 3 cut(s) 71, 327, 429
SatI GCNGC 2 cut(s) 700, 932
Sau3AI GATC 3 cut(s) 358, 616, 625
Sau96I GGNCC 2 cut(s) 505, 1017
SchI GAGTC 1 cut(s) 850
ScrFI CCNGG 2 cut(s) 342, 877
SduI GDGCHC 1 cut(s) 913
SfaNI GCATC 2 cut(s) 370, 811
SinI GGWCC 2 cut(s) 505, 1017
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 2 cut(s) 370, 491
SmoI CTYRAG 2 cut(s) 370, 491
SpeI ACTAGT 1 cut(s) 745
SphI GCATGC 1 cut(s) 1065
Sse9I AATT 6 cut(s) 41, 256, 312, 440, 665, 868
SsiI CCGC 1 cut(s) 1065
SspMI CTAG 4 cut(s) 746, 770, 861, 1040
StyD4I CCNGG 2 cut(s) 340, 875
StyI CCWWGG 1 cut(s) 860
TaaI ACNGT 4 cut(s) 555, 641, 767, 1058
TaiI ACGT 3 cut(s) 394, 420, 829
TaqI TCGA 1 cut(s) 78
TasI AATT 6 cut(s) 41, 256, 312, 440, 665, 868
TatI WGTACW 2 cut(s) 302, 1086
TfiI GAWTC 1 cut(s) 346
Tru1I TTAA 3 cut(s) 71, 327, 429
Tru9I TTAA 3 cut(s) 71, 327, 429
TscAI CASTG 2 cut(s) 58, 560
TseFI GTSAC 2 cut(s) 468, 728
TseI GCWGC 2 cut(s) 699, 931
Tsp45I GTSAC 2 cut(s) 468, 728
TspGWI ACGGA 2 cut(s) 173, 368
TspRI CASTG 2 cut(s) 58, 560
VpaK11BI GGWCC 2 cut(s) 505, 1017
XagI CCTNNNNNAGG 1 cut(s) 71
XapI RAATTY 1 cut(s) 440
XbaI TCTAGA 1 cut(s) 1039
XceI RCATGY 3 cut(s) 303, 408, 1065
XmaJI CCTAGG 1 cut(s) 860
XspI CTAG 4 cut(s) 746, 770, 861, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.