RchiOBHm_Chr1g0368321

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001926
Physical Location & Seq
Forward (+)
58495164 .. 58500322
5159 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59268

Sequence Viewer

Length: 1431 bp
ATGGAGAATACTCTCAGGGAGATAATACGTGTGCTTGAACCCTTGCCCACGGATCGAGAAACACGATGCCAAATTCTTCGGGAGTTACAAGGAGCTGTTGAGTCTGTAGAGAGCTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTTGTCTCAGAACTCTTTACTCGCTGGGGAGACTTGGATGTTTCAATTGAGTTCTCAAAAGGCGCATATATTTCTTCTTATGGAAGAAAGCACACACAAGATGTACTAAAAGATTTAATGAAGGCTATGAGAATGAGAGGTGGATGGAACAGGTATCAGTTCATACCTAATGCAAGAGTTCCCATTCTGATGGTCAAAAGTAATCTTGGAAACATCTCCTGTGATATTTCAGTCAATAACCTGAAAGCCCAAATGAAATCAAAACTCTTTTTATGGATGAATGATATAGATCCACGCTTTCGTGATATGGTTTTACTGGTGAAGGAATGGGCAAAAGCACACAATATCAATAATTCAAAATCTGGGACTTTCAATTCATACTCTCTGAGTTTGCTTGTGATCTTCCATTTTCAGACATGTACACCTGCAATTCTGCCTCCTCTTCAAGACATATATACAGATACTCTCACGGATGATCTTAAAGGCTTGAGGGATGATGCAGAAAGACGTATTGAAAAAAGTTGTGTTGAGAACATGAGAAGATTTAAACTAAACAAATTGAGAGCTCGGAATAAAAGTTCTTTGTCTGAGCTTTTCATTTCATTCCTTGGGAAGTTCTCTGACCTTGGTTCGAAGGCTGCGGAGTTGGGAATTTGCACATATTCTGGAAAATGGGAAAGAATAGAAAGCAATATGGGATGGTTGCCCAAAACCTATTGTATATTGGTTGAAGATCCTTTTGATCGGCCAGAAAATTCTGCAAGAGGTGTCGGCCAAAAAGCATTGAAAAGGATCTCTGAAGTTTTTGAGATGAGTCGTGACAGGCTAATCGCAGTTAGTTACAATGCCTCCTTAGCCATTGAGAGTTCCCTTCTTGCCACACTAGTCCGACCACATATGCTACGTCTTATTAGCAACCCAGGTTACAATATTAAGCACCACCAAACAACTCATCCACAATATTTCAGGGCTGAGAGCTCACATTCACGAACCCCAAGACATCATTATAACGGTCCCCCGAAACCAAATGGTCCTTTTGGACGAGAGTTGGTGCCCCAAAGGGATTATAATGGCCCTCCGAAACCAAGTGGTTCTTATGGACGAGAGTTGGCAGACTCGTCCCCACAAGGACATGGACATCAACAACTACGAAAGATGGTGCACTCATCCTCACAAGTACAGCCACAAATACAGAAGCTGGTGCACTCACCATCACAATACAACCGCATATACGAAAGTCTGTGCCCTCAACCTCACGAGTACAATCACCACTTCAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

476

Amino Acids

54.57

Weight (kDa)

9.5

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 3 - 139 7.5e-21 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1155, 1215
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 1198
AciI CCGC 2 cut(s) 788, 1372
AclWI GGATC 5 cut(s) 60, 151, 431, 875, 947
AcoI YGGCCR 2 cut(s) 893, 919
AcsI RAATTY 3 cut(s) 72, 798, 901
AcuI CTGAAG 2 cut(s) 966, 1405
AfaI GTAC 4 cut(s) 252, 568, 1326, 1409
AflII CTTAAG 1 cut(s) 115
AflIII ACRYGT 2 cut(s) 28, 563
AgsI TTSAA 8 cut(s) 38, 192, 504, 520, 593, 662, 878, 934
AhlI ACTAGT 1 cut(s) 1030
AjnI CCWGG 1 cut(s) 1066
AloI GAACNNNNNNTCC 2 cut(s) 709, 741
AluBI AGCT 6 cut(s) 95, 114, 713, 739, 1125, 1345
AluI AGCT 6 cut(s) 95, 114, 713, 739, 1125, 1345
Alw21I GWGCWC 4 cut(s) 715, 1127, 1311, 1353
Alw26I GTCTC 2 cut(s) 157, 171
Alw44I GTGCAC 2 cut(s) 1307, 1349
AlwI GGATC 5 cut(s) 60, 151, 431, 875, 947
AlwNI CAGNNNCTG 1 cut(s) 1345
AoxI GGCC 3 cut(s) 893, 919, 1219
ApaLI GTGCAC 2 cut(s) 1307, 1349
ApeKI GCWGC 1 cut(s) 785
ApoI RAATTY 3 cut(s) 72, 798, 901
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 3 cut(s) 1160, 1178, 1220
AsuHPI GGTGA 3 cut(s) 478, 1347, 1406
AsuII TTCGAA 1 cut(s) 779
AvaII GGWCC 2 cut(s) 1160, 1178
BaeGI GKGCMC 4 cut(s) 1203, 1311, 1353, 1394
BanI GGYRCC 1 cut(s) 1198
BanII GRGCYC 2 cut(s) 715, 1127
BauI CACGAG 1 cut(s) 1403
Bbv12I GWGCWC 4 cut(s) 715, 1127, 1311, 1353
BbvI GCAGC 1 cut(s) 772
BccI CCATC 5 cut(s) 285, 331, 840, 1297, 1366
BciT130I CCWGG 1 cut(s) 1068
BcoDI GTCTC 2 cut(s) 157, 171
BcuI ACTAGT 1 cut(s) 1030
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 1 cut(s) 786
BlsI GCNGC 1 cut(s) 787
Bme1390I CCNGG 1 cut(s) 1068
Bme18I GGWCC 2 cut(s) 1160, 1178
BmgT120I GGNCC 3 cut(s) 1160, 1178, 1220
BmiI GGNNCC 3 cut(s) 135, 1162, 1200
BmrFI CCNGG 1 cut(s) 1068
BmsI GCATC 2 cut(s) 56, 634
Bpu10I CCTNAGC 1 cut(s) 1000
Bpu14I TTCGAA 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 655
BsaAI YACGTR 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 4 cut(s) 48, 754, 772, 1066
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bse1I ACTGG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 1 cut(s) 1068
BseDI CCNNGG 4 cut(s) 48, 754, 772, 1066
BseGI GGATG 8 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1313
BseJI GATNNNNATC 1 cut(s) 435
BseMII CTCAG 5 cut(s) 28, 168, 524, 726, 1110
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 4 cut(s) 1203, 1311, 1353, 1394
BseXI GCAGC 1 cut(s) 772
BseYI CCCAGC 1 cut(s) 171
BshFI GGCC 3 cut(s) 895, 921, 1221
BshNI GGYRCC 1 cut(s) 1198
BsiHKAI GWGCWC 4 cut(s) 715, 1127, 1311, 1353
BslFI GGGAC 3 cut(s) 526, 1146, 1252
BsmAI GTCTC 2 cut(s) 157, 171
BsmFI GGGAC 3 cut(s) 526, 1146, 1252
BsnI GGCC 3 cut(s) 895, 921, 1221
Bsp119I TTCGAA 1 cut(s) 779
Bsp1286I GDGCHC 6 cut(s) 715, 1127, 1203, 1311, 1353, 1394
Bsp1407I TGTACA 1 cut(s) 566
Bsp143I GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BspACI CCGC 2 cut(s) 788, 1372
BspANI GGCC 3 cut(s) 895, 921, 1221
BspCNI CTCAG 5 cut(s) 27, 167, 525, 727, 1111
BspLI GGNNCC 3 cut(s) 135, 1162, 1200
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 5 cut(s) 60, 151, 431, 875, 947
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 1 cut(s) 1198
BspTI CTTAAG 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 4 cut(s) 48, 754, 772, 1066
BssMI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BssSI CACGAG 1 cut(s) 1403
BssT1I CCWWGG 2 cut(s) 754, 772
Bst2BI CACGAG 1 cut(s) 1403
Bst2UI CCWGG 1 cut(s) 1068
Bst4CI ACNGT 2 cut(s) 130, 1160
Bst6I CTCTTC 1 cut(s) 594
BstAFI CTTAAG 1 cut(s) 115
BstAUI TGTACA 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 779
BstDEI CTNAG 6 cut(s) 14, 154, 533, 735, 1000, 1119
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 8 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1313
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 8 cut(s) 55, 146, 439, 549, 625, 883, 892, 942
BstMAI GTCTC 2 cut(s) 157, 171
BstMBI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 1 cut(s) 1068
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 1 cut(s) 1066
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 4 cut(s) 1203, 1311, 1353, 1394
BstV1I GCAGC 1 cut(s) 772
BstX2I RGATCY 3 cut(s) 436, 880, 939
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 436, 880, 939
BsuRI GGCC 3 cut(s) 895, 921, 1221
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 8 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1313
BveI ACCTGC 1 cut(s) 580
CaiI CAGNNNCTG 1 cut(s) 1345
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 3 cut(s) 1160, 1178, 1220
Csp6I GTAC 4 cut(s) 251, 567, 1325, 1408
CviAII CATG 3 cut(s) 564, 682, 1280
CviQI GTAC 4 cut(s) 251, 567, 1325, 1408
DdeI CTNAG 6 cut(s) 14, 154, 533, 735, 1000, 1119
DpnI GATC 8 cut(s) 54, 145, 438, 548, 624, 882, 891, 941
DpnII GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 2 cut(s) 893, 919
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 2 cut(s) 713, 1125
Eco130I CCWWGG 2 cut(s) 754, 772
Eco24I GRGCYC 2 cut(s) 715, 1127
Eco47I GGWCC 2 cut(s) 1160, 1178
Eco53kI GAGCTC 2 cut(s) 713, 1125
Eco57I CTGAAG 2 cut(s) 966, 1405
EcoICRI GAGCTC 2 cut(s) 713, 1125
EcoRII CCWGG 1 cut(s) 1066
EcoT14I CCWWGG 2 cut(s) 754, 772
EcoT38I GRGCYC 2 cut(s) 715, 1127
ErhI CCWWGG 2 cut(s) 754, 772
FaeI CATG 3 cut(s) 567, 685, 1283
FalI AAGNNNNNCTT 2 cut(s) 1225, 1257
FaqI GGGAC 3 cut(s) 526, 1146, 1252
FatI CATG 3 cut(s) 563, 681, 1279
FauNDI CATATG 1 cut(s) 1044
Fnu4HI GCNGC 1 cut(s) 786
FokI GGATG 8 cut(s) 197, 303, 436, 632, 653, 858, 1086, 1300
FriOI GRGCYC 2 cut(s) 715, 1127
Fsp4HI GCNGC 1 cut(s) 786
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 786
GsaI CCCAGC 1 cut(s) 175
HaeIII GGCC 3 cut(s) 895, 921, 1221
HhaI GCGC 1 cut(s) 212
Hin1II CATG 3 cut(s) 567, 685, 1283
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HinfI GANTC 3 cut(s) 101, 961, 1262
HphI GGTGA 3 cut(s) 478, 1347, 1406
Hpy166II GTNNAC 3 cut(s) 569, 1309, 1351
Hpy188III TCNNGA 8 cut(s) 56, 80, 449, 593, 813, 965, 1134, 1403
Hpy8I GTNNAC 3 cut(s) 569, 1309, 1351
HpyAV CCTTC 4 cut(s) 262, 463, 775, 1028
HpyCH4III ACNGT 2 cut(s) 130, 1160
HpyCH4IV ACGT 3 cut(s) 28, 655, 1051
HpyCH4V TGCA 8 cut(s) 125, 320, 575, 647, 804, 908, 1309, 1351
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 6 cut(s) 14, 154, 533, 735, 1000, 1119
HpySE526I ACGT 3 cut(s) 28, 655, 1051
Hsp92II CATG 3 cut(s) 567, 685, 1283
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
LmnI GCTCC 2 cut(s) 92, 133
Lsp1109I GCAGC 1 cut(s) 772
LweI GCATC 2 cut(s) 56, 634
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 3 cut(s) 28, 655, 1051
MaeIII GTNAC 4 cut(s) 84, 965, 986, 1070
MalI GATC 8 cut(s) 54, 145, 438, 548, 624, 882, 891, 941
MboI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
MboII GAAGA 7 cut(s) 68, 213, 243, 541, 581, 699, 890
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 3 cut(s) 436, 880, 939
MhlI GDGCHC 6 cut(s) 715, 1127, 1203, 1311, 1353, 1394
MluCI AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 901
MlyI GAGTC 3 cut(s) 110, 970, 1256
MmeI TCCRAC 1 cut(s) 1060
MseI TTAA 5 cut(s) 116, 263, 627, 693, 1080
MslI CAYNNNNRTG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 115
MspR9I CCNGG 1 cut(s) 1068
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 1068
MwoI GCNNNNNNNGC 1 cut(s) 1001
NdeI CATATG 1 cut(s) 1044
NdeII GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
NlaIII CATG 3 cut(s) 567, 685, 1283
NlaIV GGNNCC 3 cut(s) 135, 1162, 1200
NmuCI GTSAC 1 cut(s) 965
NspI RCATGY 1 cut(s) 567
NspV TTCGAA 1 cut(s) 779
PaqCI CACCTGC 1 cut(s) 580
PciI ACATGT 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PkrI GCNGC 1 cut(s) 787
PleI GAGTC 3 cut(s) 109, 969, 1256
PpsI GAGTC 3 cut(s) 109, 969, 1256
Ppu21I YACGTR 1 cut(s) 29
PscI ACATGT 1 cut(s) 563
PsiI TTATAA 2 cut(s) 1155, 1215
Psp124BI GAGCTC 2 cut(s) 715, 1127
Psp6I CCWGG 1 cut(s) 1066
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 1 cut(s) 1066
PspN4I GGNNCC 3 cut(s) 135, 1162, 1200
PspPI GGNCC 3 cut(s) 1160, 1178, 1220
PstNI CAGNNNCTG 1 cut(s) 1345
PsuI RGATCY 3 cut(s) 436, 880, 939
RsaI GTAC 4 cut(s) 252, 568, 1326, 1409
RsaNI GTAC 4 cut(s) 251, 567, 1325, 1408
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 715, 1127
SaqAI TTAA 5 cut(s) 116, 263, 627, 693, 1080
SatI GCNGC 1 cut(s) 786
Sau3AI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
Sau96I GGNCC 3 cut(s) 1160, 1178, 1220
SchI GAGTC 3 cut(s) 110, 970, 1256
ScrFI CCNGG 1 cut(s) 1068
SduI GDGCHC 6 cut(s) 715, 1127, 1203, 1311, 1353, 1394
SfaNI GCATC 2 cut(s) 56, 634
SfcI CTRYAG 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 2 cut(s) 1160, 1178
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 115, 634
SmoI CTYRAG 2 cut(s) 115, 634
SpeI ACTAGT 1 cut(s) 1030
Sse9I AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 901
SsiI CCGC 2 cut(s) 788, 1372
SspI AATATT 2 cut(s) 1078, 1109
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 2 cut(s) 715, 1127
StyD4I CCNGG 1 cut(s) 1066
StyI CCWWGG 2 cut(s) 754, 772
TaaI ACNGT 2 cut(s) 130, 1160
TaiI ACGT 3 cut(s) 31, 658, 1054
TaqI TCGA 2 cut(s) 55, 779
TasI AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 901
TatI WGTACW 4 cut(s) 250, 566, 1324, 1407
Tru1I TTAA 5 cut(s) 116, 263, 627, 693, 1080
Tru9I TTAA 5 cut(s) 116, 263, 627, 693, 1080
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 1 cut(s) 785
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 7 cut(s) 281, 298, 416, 440, 513, 733, 738
TspGWI ACGGA 2 cut(s) 65, 632
Vha464I CTTAAG 1 cut(s) 115
VneI GTGCAC 2 cut(s) 1307, 1349
VpaK11BI GGWCC 2 cut(s) 1160, 1178
XapI RAATTY 3 cut(s) 72, 798, 901
XceI RCATGY 1 cut(s) 567
XspI CTAG 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.