Rw1G030950

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001926
Physical Location & Seq
Forward (+)
58954250 .. 58958286
4037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G030950.1

Sequence Viewer

Length: 1683 bp
ATGGAGAATACTCTCAGGGAGATAATACGTGTGCTTGAACCCTTGCCCACGGATCGAGAAACACGATGCCAAATTCTTCGGGAGTTACAAGGAGCTGTTGAGTCTGTAGAAAGCTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTTGTCTCAGAACTCTTTACTCGCTGGGGAGACTTGGATGTTTCAATTGAGTTCTCAAAAGGCGCATATATTTCTTCTTATGGAAGAAAGCACACACAGGATGTACTAAAAGATTTAATGAAGGCTATGAGAATGAGAGGTGGATGGAACAGGTATCAGTTCATACCTAATGCAAGAGTTCCCATTCTGATGGTCAAAAGTAATCTTGGAAACATCTCCTGTGATATTTCAGTCAATAACCAGAAAGCCCAAATGAAATCAAAACTCTTTTTATGGATGAATGATATAGATCCACGCTTTCGTGATATGGTTTTACTGGTGAAGGAATGGGCAAAAGCACACAATATCAATAATTCAAAATCTGGGACTTTCAATTCATACTCTCTGAGTTTGCTTGTGATCTTCCATTTTCAGACATGTACACCTGCAATTCTGCCTCCTCTTCAAGACATATATACAGAAACTCTCACGGATGATCTTAAAGGCTTGAGGGATGATGCAGAAAGACGTATTGAAAAAAGTTGTGTTGAGAACATGAGAAGATTTAAACTAAACAAATTGAGAGCTCAGAATAAAAGTTCTTTGTCTGAGCTTTTCATTTCATTCCTTGGGAAGTTCTCTGACCTTGGTTCGAAGGCTGCGGAGTTGGGAATTTGCACATATTCTGGAAAATGGGAAAGAATAGAAAGCAATATGGGATGGTTGCCCAAAACCTATTGTATATTGGTTGAAGATCCTTTTGATCGGCCAGAAAATTCTGCAAGAGGTGTCAGCCCAAAAGCATTGAAAAGGATCTCTGAAGTTTTTGAGATGAGTTGTGACAGGCTAATCGCAGTTAGTTACAATGCCTCCTTAGCCATTGAGAGTTCCCTTCTTGCCACACTAGTCCGACCACATACGCTACGTCTTATTAGCAACCCAGGTTACAATATTAAGCACCACCAAACAACTCATCCACAATATTTCAGGGCTGAGAGCTCACATTCACGAACCCCAAGACATCATTATAACGGTCCCCCGACACCAAATGGTCCTTTTGTACGAGACTTGGTGCCCCCAAGGGAATATAACGGCCCCCCAAAACCAAATGGTAAGGTACGAGAGTTGGTGCCCCAAACGGATTATAATGGCCCTCCGAAACCAAGTGGTTCTTATGGACGAGAGTTGGCAGACTCGTCCCCACAAGGACATGGACATCAACAACTACGAAAGATGGTGCACTCATCCTCACAAGTACAGCCACAAATACAGAAGCTGGTGCACTCACCGTCACAAGTACAGCCACAAATACAGAAGCTGGTACACTCACCGTCACAAGTACAGCCACAAATACAGAAGCTGGTGCACTCACCGTCACAAGTACAGCCACAAATACAGAAGCTGGTGCACTCACCGTCACAAGTACAACCGCATATACGAAAGTCTGTGCCCTCAACCTCACGAGTACAATCACCATTTCAGAAAGTGAAGCTGGCACCAATGCTACAGGCGTATCAGAAATGGACACCAAAGAAACCAGGAATGCAGACCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

560

Amino Acids

63.59

Weight (kDa)

9.72

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 3 - 139 7.6e-21 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1155, 1272
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 3 cut(s) 1198, 1255, 1621
AciI CCGC 2 cut(s) 788, 1556
AclWI GGATC 5 cut(s) 60, 151, 431, 875, 947
AcoI YGGCCR 1 cut(s) 893
AcsI RAATTY 3 cut(s) 72, 798, 901
AcuI CTGAAG 1 cut(s) 966
AflII CTTAAG 1 cut(s) 115
AflIII ACRYGT 2 cut(s) 28, 563
AgsI TTSAA 8 cut(s) 38, 192, 504, 520, 593, 662, 878, 934
AhlI ACTAGT 1 cut(s) 1030
AjnI CCWGG 2 cut(s) 1066, 1663
Alw21I GWGCWC 6 cut(s) 715, 1127, 1368, 1410, 1494, 1536
Alw26I GTCTC 3 cut(s) 157, 171, 1185
Alw44I GTGCAC 4 cut(s) 1364, 1406, 1490, 1532
AlwI GGATC 5 cut(s) 60, 151, 431, 875, 947
AlwNI CAGNNNCTG 4 cut(s) 1402, 1444, 1486, 1528
AoxI GGCC 3 cut(s) 893, 1219, 1276
ApaLI GTGCAC 4 cut(s) 1364, 1406, 1490, 1532
ApeKI GCWGC 1 cut(s) 785
ApoI RAATTY 3 cut(s) 72, 798, 901
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 4 cut(s) 1160, 1178, 1220, 1277
AsuHPI GGTGA 6 cut(s) 478, 1404, 1446, 1488, 1530, 1590
AsuII TTCGAA 1 cut(s) 779
AvaII GGWCC 2 cut(s) 1160, 1178
BaeGI GKGCMC 7 cut(s) 1203, 1260, 1368, 1410, 1494, 1536, 1578
BaeI ACNNNNGTAYC 4 cut(s) 1235, 1268, 1622, 1655
BanI GGYRCC 3 cut(s) 1198, 1255, 1621
BanII GRGCYC 2 cut(s) 715, 1127
BauI CACGAG 1 cut(s) 1587
Bbv12I GWGCWC 6 cut(s) 715, 1127, 1368, 1410, 1494, 1536
BbvI GCAGC 1 cut(s) 772
BccI CCATC 4 cut(s) 285, 331, 840, 1354
BceAI ACGGC 1 cut(s) 1234
BciT130I CCWGG 2 cut(s) 1068, 1665
BcoDI GTCTC 3 cut(s) 157, 171, 1185
BcuI ACTAGT 1 cut(s) 1030
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 2 cut(s) 105, 1631
BfrI CTTAAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 1 cut(s) 786
BlsI GCNGC 1 cut(s) 787
Bme1390I CCNGG 2 cut(s) 1068, 1665
Bme18I GGWCC 2 cut(s) 1160, 1178
BmgT120I GGNCC 4 cut(s) 1160, 1178, 1220, 1277
BmiI GGNNCC 6 cut(s) 135, 1162, 1200, 1222, 1257, 1623
BmrFI CCNGG 2 cut(s) 1068, 1665
BmsI GCATC 2 cut(s) 56, 634
Bpu10I CCTNAGC 1 cut(s) 1000
Bpu14I TTCGAA 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 655
BsaAI YACGTR 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 5 cut(s) 48, 754, 772, 1066, 1205
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bse1I ACTGG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 2 cut(s) 1068, 1665
BseDI CCNNGG 5 cut(s) 48, 754, 772, 1066, 1205
BseGI GGATG 9 cut(s) 190, 253, 296, 429, 625, 646, 851, 1099, 1370
BseJI GATNNNNATC 1 cut(s) 435
BseMII CTCAG 6 cut(s) 28, 168, 524, 726, 728, 1110
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 7 cut(s) 1203, 1260, 1368, 1410, 1494, 1536, 1578
BseXI GCAGC 1 cut(s) 772
BseYI CCCAGC 1 cut(s) 171
BshFI GGCC 3 cut(s) 895, 1221, 1278
BshNI GGYRCC 3 cut(s) 1198, 1255, 1621
BsiHKAI GWGCWC 6 cut(s) 715, 1127, 1368, 1410, 1494, 1536
BslFI GGGAC 3 cut(s) 526, 1146, 1309
BsmAI GTCTC 3 cut(s) 157, 171, 1185
BsmFI GGGAC 3 cut(s) 526, 1146, 1309
BsmI GAATGC 1 cut(s) 1674
BsnI GGCC 3 cut(s) 895, 1221, 1278
Bsp119I TTCGAA 1 cut(s) 779
Bsp1286I GDGCHC 9 cut(s) 715, 1127, 1203, 1260, 1368, 1410, 1494, 1536, 1578
Bsp1407I TGTACA 1 cut(s) 566
Bsp143I GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BspACI CCGC 2 cut(s) 788, 1556
BspANI GGCC 3 cut(s) 895, 1221, 1278
BspCNI CTCAG 6 cut(s) 27, 167, 525, 727, 727, 1111
BspLI GGNNCC 6 cut(s) 135, 1162, 1200, 1222, 1257, 1623
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 5 cut(s) 60, 151, 431, 875, 947
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 3 cut(s) 1198, 1255, 1621
BspTI CTTAAG 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 5 cut(s) 48, 754, 772, 1066, 1205
BssMI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BssSI CACGAG 1 cut(s) 1587
BssT1I CCWWGG 3 cut(s) 754, 772, 1205
Bst2BI CACGAG 1 cut(s) 1587
Bst2UI CCWGG 2 cut(s) 1068, 1665
Bst4CI ACNGT 6 cut(s) 130, 1160, 1416, 1458, 1500, 1542
Bst6I CTCTTC 1 cut(s) 594
BstAFI CTTAAG 1 cut(s) 115
BstAUI TGTACA 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 779
BstC8I GCNNGC 1 cut(s) 1620
BstDEI CTNAG 7 cut(s) 14, 154, 533, 714, 735, 1000, 1119
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 9 cut(s) 190, 253, 296, 429, 625, 646, 851, 1099, 1370
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 8 cut(s) 55, 146, 439, 549, 625, 883, 892, 942
BstMAI GTCTC 3 cut(s) 157, 171, 1185
BstMBI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 2 cut(s) 1068, 1665
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 2 cut(s) 1066, 1663
BstSFI CTRYAG 2 cut(s) 105, 1631
BstSLI GKGCMC 7 cut(s) 1203, 1260, 1368, 1410, 1494, 1536, 1578
BstV1I GCAGC 1 cut(s) 772
BstX2I RGATCY 3 cut(s) 436, 880, 939
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 436, 880, 939
BsuRI GGCC 3 cut(s) 895, 1221, 1278
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 9 cut(s) 190, 253, 296, 429, 625, 646, 851, 1099, 1370
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 1 cut(s) 1620
CaiI CAGNNNCTG 4 cut(s) 1402, 1444, 1486, 1528
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 4 cut(s) 1160, 1178, 1220, 1277
CviAII CATG 3 cut(s) 564, 682, 1337
DdeI CTNAG 7 cut(s) 14, 154, 533, 714, 735, 1000, 1119
DpnI GATC 8 cut(s) 54, 145, 438, 548, 624, 882, 891, 941
DpnII GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 893
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 2 cut(s) 713, 1125
Eco130I CCWWGG 3 cut(s) 754, 772, 1205
Eco24I GRGCYC 2 cut(s) 715, 1127
Eco47I GGWCC 2 cut(s) 1160, 1178
Eco53kI GAGCTC 2 cut(s) 713, 1125
Eco57I CTGAAG 1 cut(s) 966
EcoICRI GAGCTC 2 cut(s) 713, 1125
EcoRII CCWGG 2 cut(s) 1066, 1663
EcoT14I CCWWGG 3 cut(s) 754, 772, 1205
EcoT38I GRGCYC 2 cut(s) 715, 1127
ErhI CCWWGG 3 cut(s) 754, 772, 1205
FaeI CATG 3 cut(s) 567, 685, 1340
FalI AAGNNNNNCTT 2 cut(s) 1282, 1314
FaqI GGGAC 3 cut(s) 526, 1146, 1309
FatI CATG 3 cut(s) 563, 681, 1336
Fnu4HI GCNGC 1 cut(s) 786
FokI GGATG 9 cut(s) 197, 260, 303, 436, 632, 653, 858, 1086, 1357
FriOI GRGCYC 2 cut(s) 715, 1127
Fsp4HI GCNGC 1 cut(s) 786
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 786
GsaI CCCAGC 1 cut(s) 175
HaeIII GGCC 3 cut(s) 895, 1221, 1278
HhaI GCGC 1 cut(s) 212
Hin1II CATG 3 cut(s) 567, 685, 1340
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 2 cut(s) 101, 1319
HphI GGTGA 6 cut(s) 478, 1404, 1446, 1488, 1530, 1590
Hpy166II GTNNAC 6 cut(s) 569, 1366, 1408, 1450, 1492, 1534
Hpy188III TCNNGA 7 cut(s) 56, 80, 449, 593, 813, 1134, 1587
Hpy8I GTNNAC 6 cut(s) 569, 1366, 1408, 1450, 1492, 1534
HpyAV CCTTC 4 cut(s) 262, 463, 775, 1028
HpyCH4III ACNGT 6 cut(s) 130, 1160, 1416, 1458, 1500, 1542
HpyCH4IV ACGT 3 cut(s) 28, 655, 1051
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 7 cut(s) 14, 154, 533, 714, 735, 1000, 1119
HpySE526I ACGT 3 cut(s) 28, 655, 1051
Hsp92II CATG 3 cut(s) 567, 685, 1340
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
LmnI GCTCC 2 cut(s) 92, 133
Lsp1109I GCAGC 1 cut(s) 772
LweI GCATC 2 cut(s) 56, 634
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 3 cut(s) 28, 655, 1051
MaeIII GTNAC 8 cut(s) 84, 965, 986, 1070, 1416, 1458, 1500, 1542
MalI GATC 8 cut(s) 54, 145, 438, 548, 624, 882, 891, 941
MboI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
MboII GAAGA 7 cut(s) 68, 213, 243, 541, 581, 699, 890
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 3 cut(s) 436, 880, 939
MhlI GDGCHC 9 cut(s) 715, 1127, 1203, 1260, 1368, 1410, 1494, 1536, 1578
MluCI AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 901
MlyI GAGTC 2 cut(s) 110, 1313
MmeI TCCRAC 1 cut(s) 1060
MseI TTAA 5 cut(s) 116, 263, 627, 693, 1080
MslI CAYNNNNRTG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 1068, 1665
MunI CAATTG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 1674
MvaI CCWGG 2 cut(s) 1068, 1665
MwoI GCNNNNNNNGC 1 cut(s) 1001
NdeII GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
NlaIII CATG 3 cut(s) 567, 685, 1340
NlaIV GGNNCC 6 cut(s) 135, 1162, 1200, 1222, 1257, 1623
NmuCI GTSAC 5 cut(s) 965, 1416, 1458, 1500, 1542
NspI RCATGY 1 cut(s) 567
NspV TTCGAA 1 cut(s) 779
PaqCI CACCTGC 1 cut(s) 580
PciI ACATGT 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PctI GAATGC 1 cut(s) 1674
PkrI GCNGC 1 cut(s) 787
PleI GAGTC 2 cut(s) 109, 1313
PpsI GAGTC 2 cut(s) 109, 1313
Ppu21I YACGTR 1 cut(s) 29
PscI ACATGT 1 cut(s) 563
PsiI TTATAA 2 cut(s) 1155, 1272
Psp124BI GAGCTC 2 cut(s) 715, 1127
Psp6I CCWGG 2 cut(s) 1066, 1663
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 2 cut(s) 1066, 1663
PspN4I GGNNCC 6 cut(s) 135, 1162, 1200, 1222, 1257, 1623
PspPI GGNCC 4 cut(s) 1160, 1178, 1220, 1277
PstNI CAGNNNCTG 4 cut(s) 1402, 1444, 1486, 1528
PsuI RGATCY 3 cut(s) 436, 880, 939
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 715, 1127
SaqAI TTAA 5 cut(s) 116, 263, 627, 693, 1080
SatI GCNGC 1 cut(s) 786
Sau3AI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
Sau96I GGNCC 4 cut(s) 1160, 1178, 1220, 1277
SchI GAGTC 2 cut(s) 110, 1313
ScrFI CCNGG 2 cut(s) 1068, 1665
SduI GDGCHC 9 cut(s) 715, 1127, 1203, 1260, 1368, 1410, 1494, 1536, 1578
SfaNI GCATC 2 cut(s) 56, 634
SfcI CTRYAG 2 cut(s) 105, 1631
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 2 cut(s) 1160, 1178
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 115, 634
SmoI CTYRAG 2 cut(s) 115, 634
SpeI ACTAGT 1 cut(s) 1030
Sse9I AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 901
SsiI CCGC 2 cut(s) 788, 1556
SspI AATATT 2 cut(s) 1078, 1109
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 2 cut(s) 715, 1127
StyD4I CCNGG 2 cut(s) 1066, 1663
StyI CCWWGG 3 cut(s) 754, 772, 1205
TaaI ACNGT 6 cut(s) 130, 1160, 1416, 1458, 1500, 1542
TaiI ACGT 3 cut(s) 31, 658, 1054
TaqI TCGA 2 cut(s) 55, 779
TasI AATT 8 cut(s) 72, 192, 499, 520, 576, 704, 798, 901
TatI WGTACW 8 cut(s) 250, 566, 1381, 1423, 1465, 1507, 1549, 1591
Tru1I TTAA 5 cut(s) 116, 263, 627, 693, 1080
Tru9I TTAA 5 cut(s) 116, 263, 627, 693, 1080
TseFI GTSAC 5 cut(s) 965, 1416, 1458, 1500, 1542
TseI GCWGC 1 cut(s) 785
Tsp45I GTSAC 5 cut(s) 965, 1416, 1458, 1500, 1542
TspDTI ATGAA 7 cut(s) 281, 298, 416, 440, 513, 733, 738
TspGWI ACGGA 3 cut(s) 65, 632, 1280
Vha464I CTTAAG 1 cut(s) 115
VneI GTGCAC 4 cut(s) 1364, 1406, 1490, 1532
VpaK11BI GGWCC 2 cut(s) 1160, 1178
XapI RAATTY 3 cut(s) 72, 798, 901
XceI RCATGY 1 cut(s) 567
XspI CTAG 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.