Rorug01G0340600

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001926
Physical Location & Seq
Forward (+)
45880647 .. 45883893
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0340600.1

Sequence Viewer

Length: 852 bp
ATGGCGAAGGACGTTGAAGTTGCCGAGCGGGGTTCCTTCGCAGCCAAGGACTACCATGACCCGCCTCCAGCTCCGCTCTTCGACTCGGTTGAGCTGACCAAATGGTCGTTTTACAGAGCTGTTATAGCTGAGTTCATAGCAACCCTTCTGTTTCTCTACATCACTGTGCTGACTGTGATCGGGTACAAGTCCCAAAGCGAGACCGACGACTGCGGCGGAGTTGGGATTTTGGGTATTGCCTGGGCCTTTGGAGGCATGATCTTCATCCTCGTTTACTGCACTGCTGGAATCTCAGGGGGTCACATTAACCCGGCTGTGACTTTTGGGCTGTTCTTGGCCCGGAAGGTGTCGCTGGTCCGAGCTGTGATGTACATAGTGGCTCAGAGTTTGGGTGCCATAGCCGGAGTTGGGCTAGTCAAGGCTTTCCAGAGTTCTTACTATAAAAAGTTTGGCGGTGGAGCCAATACGCTTGCTGATGGGTACAGCACTGGAGTTGGATTGTCTGCTGAGATTATTGGAACCTTTGTGCTGGTTTACACTGTTTTCTCTGCCACTGACCCAAAGAGGAGTGCTAGAGATTCCCATGTTCCGGTTTTGGCACCACTTCCAATTGGGTTTGCTGTGTTCATGGTTCACTTGGCCACCATCCCAATCACCGGCACTGGCATCAACCCCGCTAGAAGTCTTGGAGCTGCAGTTATCTTCGACGACAAGAAGGCTTGGGATGACCAGTGGATCTTCTGGGTTGGACCCTTTATTGGTGCAGCAATTGCAGCTTTCTACCACCAGTTCATCTTGAGAGCAGGAGCTATTAAGGCGCTAGGGTCATTCAGGAGCAACCCCCATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

283

Amino Acids

30.19

Weight (kDa)

8.44

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 31 - 262 9.7e-83 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 103
AccB1I GGYRCC 2 cut(s) 392, 598
AccBSI CCGCTC 2 cut(s) 28, 76
AciI CCGC 7 cut(s) 28, 62, 74, 213, 216, 453, 675
AclWI GGATC 1 cut(s) 743
AcoI YGGCCR 1 cut(s) 639
AfaI GTAC 3 cut(s) 185, 371, 482
AfiI CCNNNNNNNGG 4 cut(s) 408, 589, 656, 758
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 1 cut(s) 239
AluBI AGCT 8 cut(s) 71, 94, 119, 128, 362, 692, 776, 809
AluI AGCT 8 cut(s) 71, 94, 119, 128, 362, 692, 776, 809
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 1 cut(s) 743
AoxI GGCC 3 cut(s) 243, 336, 639
ApeKI GCWGC 4 cut(s) 41, 692, 764, 773
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 4 cut(s) 243, 337, 355, 749
AsuC2I CCSGG 2 cut(s) 311, 340
AsuHPI GGTGA 1 cut(s) 646
AvaII GGWCC 2 cut(s) 355, 749
BalI TGGCCA 1 cut(s) 641
BanI GGYRCC 2 cut(s) 392, 598
BbvI GCAGC 4 cut(s) 53, 679, 776, 785
BccI CCATC 2 cut(s) 470, 653
BciT130I CCWGG 1 cut(s) 241
BcnI CCSGG 2 cut(s) 311, 340
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 4 cut(s) 413, 573, 678, 821
BfmI CTRYAG 1 cut(s) 693
BfoI RGCGCY 1 cut(s) 821
BisI GCNGC 5 cut(s) 42, 214, 693, 765, 774
BlsI GCNGC 5 cut(s) 43, 215, 694, 766, 775
Bme1390I CCNGG 3 cut(s) 241, 311, 340
Bme18I GGWCC 2 cut(s) 355, 749
BmgT120I GGNCC 4 cut(s) 243, 337, 355, 749
BmiI GGNNCC 6 cut(s) 34, 394, 460, 520, 600, 751
BmrFI CCNGG 3 cut(s) 241, 311, 340
BmsI GCATC 1 cut(s) 675
BpmI CTGGAG 2 cut(s) 51, 510
BpuEI CTTGAG 1 cut(s) 817
BpuMI CCSGG 2 cut(s) 311, 340
BsaBI GATNNNNATC 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 45, 240
BsaWI WCCGGW 1 cut(s) 589
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 4 cut(s) 408, 589, 656, 758
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 4 cut(s) 493, 667, 730, 787
Bse8I GATNNNNATC 1 cut(s) 263
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 45, 240
BseGI GGATG 3 cut(s) 264, 645, 730
BseJI GATNNNNATC 1 cut(s) 263
BseLI CCNNNNNNNGG 4 cut(s) 408, 589, 656, 758
BseMII CTCAG 4 cut(s) 120, 306, 395, 498
BseNI ACTGG 4 cut(s) 493, 667, 730, 787
BseRI GAGGAG 1 cut(s) 580
BseXI GCAGC 4 cut(s) 53, 679, 776, 785
BsgI GTGCAG 2 cut(s) 262, 783
BshFI GGCC 3 cut(s) 245, 338, 641
BshNI GGYRCC 2 cut(s) 392, 598
BsiSI CCGG 5 cut(s) 311, 340, 402, 590, 657
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 4 cut(s) 408, 589, 656, 758
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 3 cut(s) 245, 338, 641
Bso31I GGTCTC 1 cut(s) 194
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 3 cut(s) 177, 258, 735
BspACI CCGC 7 cut(s) 28, 62, 74, 213, 216, 453, 675
BspANI GGCC 3 cut(s) 245, 338, 641
BspCNI CTCAG 4 cut(s) 121, 305, 394, 499
BspLI GGNNCC 6 cut(s) 34, 394, 460, 520, 600, 751
BspMAI CTGCAG 1 cut(s) 697
BspPI GGATC 1 cut(s) 743
BspQI GCTCTTC 1 cut(s) 83
BspT107I GGYRCC 2 cut(s) 392, 598
BspTNI GGTCTC 1 cut(s) 194
BsrBI CCGCTC 2 cut(s) 28, 76
BsrFI RCCGGY 1 cut(s) 656
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 4 cut(s) 493, 667, 730, 787
BssAI RCCGGY 1 cut(s) 656
BssECI CCNNGG 2 cut(s) 45, 240
BssMI GATC 3 cut(s) 177, 258, 735
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 3 cut(s) 166, 175, 541
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 770
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 4 cut(s) 129, 292, 381, 507
BstF5I GGATG 3 cut(s) 264, 645, 730
BstH2I RGCGCY 1 cut(s) 821
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 3 cut(s) 180, 261, 738
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 3 cut(s) 177, 258, 735
BstMWI GCNNNNNNNGC 4 cut(s) 125, 770, 773, 815
BstNI CCWGG 1 cut(s) 241
BstSCI CCNGG 3 cut(s) 239, 309, 338
BstSFI CTRYAG 1 cut(s) 693
BstV1I GCAGC 4 cut(s) 53, 679, 776, 785
BstX2I RGATCY 1 cut(s) 735
BstYI RGATCY 1 cut(s) 735
BsuRI GGCC 3 cut(s) 245, 338, 641
BtsCI GGATG 3 cut(s) 264, 645, 730
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 7 cut(s) 162, 279, 486, 537, 552, 660, 737
Cac8I GCNNGC 1 cut(s) 471
CfoI GCGC 1 cut(s) 820
Cfr10I RCCGGY 1 cut(s) 656
Cfr13I GGNCC 4 cut(s) 243, 337, 355, 749
Csp6I GTAC 3 cut(s) 184, 370, 481
CviAII CATG 4 cut(s) 56, 256, 584, 628
CviQI GTAC 3 cut(s) 184, 370, 481
DdeI CTNAG 4 cut(s) 129, 292, 381, 507
DpnI GATC 3 cut(s) 179, 260, 737
DpnII GATC 3 cut(s) 177, 258, 735
DrdI GACNNNNNNGTC 1 cut(s) 103
DseDI GACNNNNNNGTC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 2 cut(s) 355, 749
EcoRII CCWGG 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 4 cut(s) 59, 259, 587, 631
FaqI GGGAC 1 cut(s) 175
FatI CATG 4 cut(s) 55, 255, 583, 627
FauI CCCGC 3 cut(s) 21, 69, 682
Fnu4HI GCNGC 5 cut(s) 42, 214, 693, 765, 774
FokI GGATG 3 cut(s) 251, 632, 737
Fsp4HI GCNGC 5 cut(s) 42, 214, 693, 765, 774
FspBI CTAG 4 cut(s) 413, 573, 678, 821
GlaI GCGC 1 cut(s) 819
GluI GCNGC 5 cut(s) 42, 214, 693, 765, 774
GsuI CTGGAG 2 cut(s) 51, 510
HaeII RGCGCY 1 cut(s) 821
HaeIII GGCC 3 cut(s) 245, 338, 641
HapII CCGG 5 cut(s) 311, 340, 402, 590, 657
HhaI GCGC 1 cut(s) 820
Hin1II CATG 4 cut(s) 59, 259, 587, 631
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HinfI GANTC 3 cut(s) 83, 288, 578
HpaII CCGG 5 cut(s) 311, 340, 402, 590, 657
HphI GGTGA 1 cut(s) 646
Hpy166II GTNNAC 3 cut(s) 274, 535, 634
Hpy188I TCNGA 2 cut(s) 359, 384
Hpy188III TCNNGA 3 cut(s) 427, 796, 832
Hpy8I GTNNAC 3 cut(s) 274, 535, 634
Hpy99I CGWCG 2 cut(s) 209, 710
HpyAV CCTTC 4 cut(s) 46, 155, 337, 709
HpyCH4III ACNGT 3 cut(s) 166, 175, 541
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 4 cut(s) 279, 695, 764, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 770, 773, 815
HpyF3I CTNAG 4 cut(s) 129, 292, 381, 507
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 4 cut(s) 59, 259, 587, 631
HspAI GCGC 1 cut(s) 818
Kzo9I GATC 3 cut(s) 177, 258, 735
LguI GCTCTTC 1 cut(s) 83
LmnI GCTCC 5 cut(s) 76, 458, 689, 806, 834
Lsp1109I GCAGC 4 cut(s) 53, 679, 776, 785
LweI GCATC 1 cut(s) 675
MaeI CTAG 4 cut(s) 413, 573, 678, 821
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 2 cut(s) 299, 316
MalI GATC 3 cut(s) 179, 260, 737
MbiI CCGCTC 2 cut(s) 28, 76
MboI GATC 3 cut(s) 177, 258, 735
MboII GAAGA 4 cut(s) 70, 253, 694, 730
MfeI CAATTG 2 cut(s) 609, 768
MflI RGATCY 1 cut(s) 735
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 3 cut(s) 609, 768, 847
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 2 cut(s) 475, 727
MnlI CCTC 4 cut(s) 75, 245, 278, 558
Mox20I TGGCCA 1 cut(s) 641
MscI TGGCCA 1 cut(s) 641
MseI TTAA 2 cut(s) 306, 813
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 641
MspI CCGG 5 cut(s) 311, 340, 402, 590, 657
MspR9I CCNGG 3 cut(s) 241, 311, 340
MunI CAATTG 2 cut(s) 609, 768
MvaI CCWGG 1 cut(s) 241
MwoI GCNNNNNNNGC 4 cut(s) 125, 770, 773, 815
NciI CCSGG 2 cut(s) 311, 340
NdeII GATC 3 cut(s) 177, 258, 735
NlaIII CATG 4 cut(s) 59, 259, 587, 631
NlaIV GGNNCC 6 cut(s) 34, 394, 460, 520, 600, 751
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 2 cut(s) 299, 316
PciSI GCTCTTC 1 cut(s) 83
PfeI GAWTC 2 cut(s) 288, 578
PkrI GCNGC 5 cut(s) 43, 215, 694, 766, 775
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 6 cut(s) 34, 394, 460, 520, 600, 751
PspPI GGNCC 4 cut(s) 243, 337, 355, 749
PstI CTGCAG 1 cut(s) 697
PsuI RGATCY 1 cut(s) 735
RsaI GTAC 3 cut(s) 185, 371, 482
RsaNI GTAC 3 cut(s) 184, 370, 481
RseI CAYNNNNRTG 1 cut(s) 164
SapI GCTCTTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 306, 813
SatI GCNGC 5 cut(s) 42, 214, 693, 765, 774
Sau3AI GATC 3 cut(s) 177, 258, 735
Sau96I GGNCC 4 cut(s) 243, 337, 355, 749
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 3 cut(s) 241, 311, 340
SfaNI GCATC 1 cut(s) 675
SfcI CTRYAG 1 cut(s) 693
SinI GGWCC 2 cut(s) 355, 749
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 796
SmoI CTYRAG 1 cut(s) 796
Sse9I AATT 3 cut(s) 609, 768, 847
SsiI CCGC 7 cut(s) 28, 62, 74, 213, 216, 453, 675
SspMI CTAG 4 cut(s) 413, 573, 678, 821
StyD4I CCNGG 3 cut(s) 239, 309, 338
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 3 cut(s) 166, 175, 541
TaiI ACGT 1 cut(s) 15
TaqI TCGA 2 cut(s) 81, 705
TaqII GACCGA 1 cut(s) 218
TasI AATT 3 cut(s) 609, 768, 847
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 2 cut(s) 288, 578
Tru1I TTAA 2 cut(s) 306, 813
Tru9I TTAA 2 cut(s) 306, 813
TscAI CASTG 7 cut(s) 169, 286, 493, 544, 559, 667, 737
TseFI GTSAC 2 cut(s) 299, 316
TseI GCWGC 4 cut(s) 41, 692, 764, 773
Tsp45I GTSAC 2 cut(s) 299, 316
TspDTI ATGAA 4 cut(s) 124, 253, 616, 781
TspRI CASTG 7 cut(s) 169, 286, 493, 544, 559, 667, 737
VpaK11BI GGWCC 2 cut(s) 355, 749
XspI CTAG 4 cut(s) 413, 573, 678, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.