RLG00000027221

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001926
Physical Location & Seq
Reverse (-)
9687729 .. 9690991
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027221

Sequence Viewer

Length: 1272 bp
ATGAATACACATGTTATGTTGGATAATACTCTCAATCAGATTCTACGAGTTCTTAAACCCTCACTGGAAGATTGGGAAACACGACTGAGAATCCTTCATGATTTACAAGGAGCTGTTGAATCTGTGGAGGGTTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTCGTGTCTAATCTGTATGCTTGCTGGGGAGACTTGGATATTTCAATTGAGTTTTCCAAAGGCTCATATATTTCAGAGTATGGGAAAAAGCACAAAGAACAATTACTAGGAGTTGTGATGAGAGCTATGAGACAGAGAGGTGGATGGAAAAGTTTTAAATTTATACCCACTGCAAGAGTTCCCATATTAAAGGTCGAAAGTTATCTCCAAAACATCTCTTGCGATATATCAATCAATAATCTGAAGGGACAAATGAAATCCAAACTCTTGTTTTGTATTGGTGAGATTGATGGGCGCTTCCGAGATATGGTTTTACTGGTAAAGGAATGGGCAAAAGCACAAAATATGAATAATCCAAGACTCGGGTCTTTCAACTCGTACGCCCTCACTTTGCTTGTGGTCTTCCACTTTCAGACTTGTACACCTGCAATTTTGCCACCTCTTAAAGATATATATCCAGGGAATCTCACGGATGATCTTCAAGGCTTGAGGGCTGATGCAGAAAAACGTATTGAAGAAACATGTGTTGATAACGTAACAAGGTTTAAATCAGACAAATTTGGAGCAGAAAACAGAAGTTCATTGTCACAGCTTTTCATTTCATTTCTTGAGAAGTTTTCGGACCTTGTTCTGAAAACAAGAAAGTTGGGAATTTGCCCATATACCGGACAGTGGGAAGACATACCAAGCAATATGACATGGTTGCCCCAAACATATACCATAATGATTGAAGATCCCTTTGATCAGCCAGAAAACTCTTCAAGGGCTGTTAGCATGGGAGAATTGACAAGGATATCTGAAGCTTTTAAGATGAGCAGCTACATACTCATGTCAGCTAATCAGAGTGACATTCTTGCCACACTAGTCCGACCCCTAACCTACCGTCTTGTGACAGGAACATCTCACTACCAGTTTGAGCATCGCCATGCAGCTTATCCACAACGTTTCATGACTGAGAGCCCGCATTCAAGAACACCAAGGGGGAATTATGTTCCCAGGCAACCACGTTGTCCTCAGGTGCATAAGTGGGCACAGAAACCCTCACAAGTCCAGCCTCAATTTCAGAAGATGAGGCCAGAAAACCATCCTGTCAAGCCCACTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

424

Amino Acids

48.54

Weight (kDa)

9.09

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 7 - 148 5.6e-22 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 598
Acc36I ACCTGC 1 cut(s) 598
AciI CCGC 1 cut(s) 1127
AclI AACGTT 1 cut(s) 1108
AclWI GGATC 2 cut(s) 169, 893
AcsI RAATTY 3 cut(s) 323, 722, 816
AcuI CTGAAG 2 cut(s) 428, 984
AfaI GTAC 2 cut(s) 545, 586
AfiI CCNNNNNNNGG 4 cut(s) 472, 527, 830, 837
AflIII ACRYGT 2 cut(s) 10, 686
AgsI TTSAA 8 cut(s) 119, 210, 538, 647, 680, 896, 927, 1134
AhlI ACTAGT 1 cut(s) 1027
AjnI CCWGG 2 cut(s) 622, 1160
AluBI AGCT 7 cut(s) 113, 290, 757, 968, 984, 1001, 1097
AluI AGCT 7 cut(s) 113, 290, 757, 968, 984, 1001, 1097
Alw26I GTCTC 2 cut(s) 189, 289
AlwI GGATC 2 cut(s) 169, 893
Ama87I CYCGRG 1 cut(s) 527
AoxI GGCC 1 cut(s) 1238
ApeKI GCWGC 2 cut(s) 981, 1094
ApoI RAATTY 3 cut(s) 323, 722, 816
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 787
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 527
AvaII GGWCC 1 cut(s) 787
AxyI CCTNAGG 1 cut(s) 1179
BaeGI GKGCMC 1 cut(s) 1198
BanII GRGCYC 1 cut(s) 1127
BbsI GAAGAC 2 cut(s) 559, 849
BbvI GCAGC 2 cut(s) 993, 1106
BccI CCATC 3 cut(s) 303, 449, 1257
BciT130I CCWGG 2 cut(s) 624, 1162
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 2 cut(s) 189, 289
BcuI ACTAGT 1 cut(s) 1027
BfaI CTAG 2 cut(s) 272, 1028
BfoI RGCGCY 1 cut(s) 463
BfuAI ACCTGC 1 cut(s) 598
BisI GCNGC 2 cut(s) 982, 1095
BlsI GCNGC 2 cut(s) 983, 1096
Bme1390I CCNGG 2 cut(s) 624, 1162
Bme18I GGWCC 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 527
BmgT120I GGNCC 1 cut(s) 787
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 624, 1162
BmsI GCATC 2 cut(s) 652, 1093
BoxI GACNNNNGTC 1 cut(s) 529
BpiI GAAGAC 2 cut(s) 559, 849
BpuEI CTTGAG 2 cut(s) 673, 794
BsaBI GATNNNNATC 1 cut(s) 618
BsaJI CCNNGG 3 cut(s) 623, 1142, 1160
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 4 cut(s) 472, 527, 830, 837
Bse1I ACTGG 3 cut(s) 69, 486, 1075
Bse21I CCTNAGG 1 cut(s) 1179
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 2 cut(s) 624, 1162
BseDI CCNNGG 3 cut(s) 623, 1142, 1160
BseGI GGATG 3 cut(s) 314, 643, 1249
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 4 cut(s) 472, 527, 830, 837
BseMII CTCAG 3 cut(s) 77, 1110, 1193
BseNI ACTGG 3 cut(s) 69, 486, 1075
BseSI GKGCMC 1 cut(s) 1198
BseXI GCAGC 2 cut(s) 993, 1106
BseYI CCCAGC 1 cut(s) 189
BshFI GGCC 1 cut(s) 1240
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 1 cut(s) 831
BsiWI CGTACG 1 cut(s) 543
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 4 cut(s) 472, 527, 830, 837
BsmAI GTCTC 2 cut(s) 189, 289
BsmFI GGGAC 1 cut(s) 426
BsmI GAATGC 1 cut(s) 1129
BsnI GGCC 1 cut(s) 1240
BsoBI CYCGRG 1 cut(s) 527
Bsp1286I GDGCHC 2 cut(s) 1127, 1198
Bsp1407I TGTACA 1 cut(s) 584
Bsp143I GATC 4 cut(s) 161, 640, 898, 907
BspACI CCGC 1 cut(s) 1127
BspANI GGCC 1 cut(s) 1240
BspCNI CTCAG 3 cut(s) 78, 1111, 1192
BspHI TCATGA 2 cut(s) 97, 1113
BspLI GGNNCC 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 2 cut(s) 169, 893
BsrGI TGTACA 1 cut(s) 584
BsrI ACTGG 3 cut(s) 69, 486, 1075
BssECI CCNNGG 3 cut(s) 623, 1142, 1160
BssMI GATC 4 cut(s) 161, 640, 898, 907
BssT1I CCWWGG 1 cut(s) 1142
Bst2UI CCWGG 2 cut(s) 624, 1162
Bst4CI ACNGT 3 cut(s) 148, 837, 1049
Bst6I CTCTTC 1 cut(s) 928
BstAUI TGTACA 1 cut(s) 584
BstC8I GCNNGC 2 cut(s) 187, 1127
BstDEI CTNAG 3 cut(s) 86, 1119, 1179
BstF5I GGATG 3 cut(s) 314, 643, 1249
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 4 cut(s) 164, 643, 901, 910
BstMAI GTCTC 2 cut(s) 189, 289
BstMBI GATC 4 cut(s) 161, 640, 898, 907
BstNI CCWGG 2 cut(s) 624, 1162
BstNSI RCATGY 2 cut(s) 14, 690
BstPAI GACNNNNGTC 1 cut(s) 529
BstSCI CCNGG 2 cut(s) 622, 1160
BstSLI GKGCMC 1 cut(s) 1198
BstV1I GCAGC 2 cut(s) 993, 1106
BstV2I GAAGAC 2 cut(s) 559, 849
BstX2I RGATCY 1 cut(s) 898
BstYI RGATCY 1 cut(s) 898
Bsu36I CCTNAGG 1 cut(s) 1179
BsuRI GGCC 1 cut(s) 1240
BtgZI GCGATG 1 cut(s) 1070
BtsCI GGATG 3 cut(s) 314, 643, 1249
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 3 cut(s) 62, 333, 842
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 187, 1127
CciI TCATGA 2 cut(s) 97, 1113
CfoI GCGC 1 cut(s) 462
Cfr13I GGNCC 1 cut(s) 787
Csp6I GTAC 2 cut(s) 544, 585
CviAII CATG 8 cut(s) 11, 98, 687, 864, 940, 994, 1091, 1114
CviQI GTAC 2 cut(s) 544, 585
DdeI CTNAG 3 cut(s) 86, 1119, 1179
DpnI GATC 4 cut(s) 163, 642, 900, 909
DpnII GATC 4 cut(s) 161, 640, 898, 907
DraI TTTAAA 2 cut(s) 322, 712
Eam1104I CTCTTC 1 cut(s) 928
EarI CTCTTC 1 cut(s) 928
Eco130I CCWWGG 1 cut(s) 1142
Eco24I GRGCYC 1 cut(s) 1127
Eco32I GATATC 1 cut(s) 960
Eco47I GGWCC 1 cut(s) 787
Eco57I CTGAAG 2 cut(s) 428, 984
Eco81I CCTNAGG 1 cut(s) 1179
Eco88I CYCGRG 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 622, 1160
EcoRV GATATC 1 cut(s) 960
EcoT14I CCWWGG 1 cut(s) 1142
EcoT38I GRGCYC 1 cut(s) 1127
ErhI CCWWGG 1 cut(s) 1142
FaeI CATG 8 cut(s) 14, 101, 690, 867, 943, 997, 1094, 1117
FaqI GGGAC 1 cut(s) 426
FatI CATG 8 cut(s) 10, 97, 686, 863, 939, 993, 1090, 1113
FauI CCCGC 1 cut(s) 1134
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 2 cut(s) 982, 1095
FokI GGATG 3 cut(s) 321, 650, 1236
FriOI GRGCYC 1 cut(s) 1127
Fsp4HI GCNGC 2 cut(s) 982, 1095
FspBI CTAG 2 cut(s) 272, 1028
GlaI GCGC 1 cut(s) 461
GluI GCNGC 2 cut(s) 982, 1095
GsaI CCCAGC 1 cut(s) 193
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 1 cut(s) 1240
HapII CCGG 1 cut(s) 831
HhaI GCGC 1 cut(s) 462
Hin1II CATG 8 cut(s) 14, 101, 690, 867, 943, 997, 1094, 1117
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 966
HinfI GANTC 5 cut(s) 40, 90, 119, 525, 628
HpaII CCGG 1 cut(s) 831
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 1 cut(s) 587
Hpy188III TCNNGA 4 cut(s) 98, 773, 1114, 1134
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 2 cut(s) 104, 403
HpyCH4III ACNGT 3 cut(s) 148, 837, 1049
HpyCH4IV ACGT 4 cut(s) 673, 699, 1108, 1171
HpyCH4V TGCA 6 cut(s) 143, 338, 593, 665, 1094, 1186
HpyF3I CTNAG 3 cut(s) 86, 1119, 1179
HpySE526I ACGT 4 cut(s) 673, 699, 1108, 1171
Hsp92II CATG 8 cut(s) 14, 101, 690, 867, 943, 997, 1094, 1117
HspAI GCGC 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 4 cut(s) 161, 640, 898, 907
LmnI GCTCC 3 cut(s) 110, 151, 728
Lsp1109I GCAGC 2 cut(s) 993, 1106
LweI GCATC 2 cut(s) 652, 1093
MaeI CTAG 2 cut(s) 272, 1028
MaeII ACGT 4 cut(s) 673, 699, 1108, 1171
MaeIII GTNAC 4 cut(s) 700, 750, 1010, 1054
MalI GATC 4 cut(s) 163, 642, 900, 909
MboI GATC 4 cut(s) 161, 640, 898, 907
MboII GAAGA 8 cut(s) 80, 559, 635, 692, 854, 908, 915, 1243
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 898
MhlI GDGCHC 2 cut(s) 1127, 1198
MluCI AATT 9 cut(s) 210, 266, 323, 594, 722, 816, 947, 1150, 1223
MlyI GAGTC 1 cut(s) 519
MmeI TCCRAC 1 cut(s) 1057
MseI TTAA 8 cut(s) 54, 134, 321, 353, 609, 711, 972, 1270
MslI CAYNNNNRTG 2 cut(s) 992, 1089
MspI CCGG 1 cut(s) 831
MspR9I CCNGG 2 cut(s) 624, 1162
MunI CAATTG 1 cut(s) 210
Mva1269I GAATGC 1 cut(s) 1129
MvaI CCWGG 2 cut(s) 624, 1162
NdeII GATC 4 cut(s) 161, 640, 898, 907
NlaIII CATG 8 cut(s) 14, 101, 690, 867, 943, 997, 1094, 1117
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 3 cut(s) 750, 1010, 1054
NspI RCATGY 2 cut(s) 14, 690
PagI TCATGA 2 cut(s) 97, 1113
PaqCI CACCTGC 1 cut(s) 598
PciI ACATGT 2 cut(s) 10, 686
PctI GAATGC 1 cut(s) 1129
PfeI GAWTC 4 cut(s) 40, 90, 119, 628
Pfl23II CGTACG 1 cut(s) 543
PkrI GCNGC 2 cut(s) 983, 1096
PleI GAGTC 1 cut(s) 519
PpsI GAGTC 1 cut(s) 519
PscI ACATGT 2 cut(s) 10, 686
PshAI GACNNNNGTC 1 cut(s) 529
Psp1406I AACGTT 1 cut(s) 1108
Psp6I CCWGG 2 cut(s) 622, 1160
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 2 cut(s) 622, 1160
PspLI CGTACG 1 cut(s) 543
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 1 cut(s) 787
PsuI RGATCY 1 cut(s) 898
RsaI GTAC 2 cut(s) 545, 586
RsaNI GTAC 2 cut(s) 544, 585
RseI CAYNNNNRTG 2 cut(s) 992, 1089
SaqAI TTAA 8 cut(s) 54, 134, 321, 353, 609, 711, 972, 1270
SatI GCNGC 2 cut(s) 982, 1095
Sau3AI GATC 4 cut(s) 161, 640, 898, 907
Sau96I GGNCC 1 cut(s) 787
SchI GAGTC 1 cut(s) 519
ScrFI CCNGG 2 cut(s) 624, 1162
SduI GDGCHC 2 cut(s) 1127, 1198
SfaNI GCATC 2 cut(s) 652, 1093
SinI GGWCC 1 cut(s) 787
SmiMI CAYNNNNRTG 2 cut(s) 992, 1089
SmlI CTYRAG 2 cut(s) 652, 773
SmoI CTYRAG 2 cut(s) 652, 773
SpeI ACTAGT 1 cut(s) 1027
Sse9I AATT 9 cut(s) 210, 266, 323, 594, 722, 816, 947, 1150, 1223
SsiI CCGC 1 cut(s) 1127
SspMI CTAG 2 cut(s) 272, 1028
StyD4I CCNGG 2 cut(s) 622, 1160
StyI CCWWGG 1 cut(s) 1142
TaaI ACNGT 3 cut(s) 148, 837, 1049
TaiI ACGT 4 cut(s) 676, 702, 1111, 1174
TaqI TCGA 1 cut(s) 360
TasI AATT 9 cut(s) 210, 266, 323, 594, 722, 816, 947, 1150, 1223
TatI WGTACW 1 cut(s) 584
TfiI GAWTC 4 cut(s) 40, 90, 119, 628
Tru1I TTAA 8 cut(s) 54, 134, 321, 353, 609, 711, 972, 1270
Tru9I TTAA 8 cut(s) 54, 134, 321, 353, 609, 711, 972, 1270
TscAI CASTG 3 cut(s) 69, 340, 842
TseFI GTSAC 3 cut(s) 750, 1010, 1054
TseI GCWGC 2 cut(s) 981, 1094
Tsp45I GTSAC 3 cut(s) 750, 1010, 1054
TspDTI ATGAA 8 cut(s) 17, 86, 434, 527, 735, 751, 756, 1102
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 3 cut(s) 69, 340, 842
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 3 cut(s) 323, 722, 816
XceI RCATGY 2 cut(s) 14, 690
XspI CTAG 2 cut(s) 272, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.