Rh1BG310600

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001926
Physical Location & Seq
Forward (+)
44554534 .. 44558347
3814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG310600.1

Sequence Viewer

Length: 1245 bp
ATGTCATTGACTCCTATGATGGTCATTCTTTACACCACAATGGACATTGATCCTCCACTTTATTCTACATTGCACTTGAAAAGATGCAGAGAAGTTAAGTTTACGGCATCTATGGAACCGATGATTGAAATTGCAAAACTAAGGAACGGTGTTGATTTTAATATAGGTGGATGGAAAAGTTTTAAATTTATACCCACTGCAAGAGTTCCCATATTAAAGGTCGAAAGTTATATCCAAAACATCTCTTGCGATATATCAATCAATAATCTGAAGGGACAAATGAAATCCAAACTCTTGTTTTGTATTGGTGAGATTGATGGGCGCTTCCGAGATATGGTTTTACTGGTAAAGGAATGGGCAAAAGCACAAAATATGAATAATCCAAGACTTGGGTCTTTCAACTCATACGCCCTCACTTTGCTTGTGGTCTTCCACTTTCAGACATGTACACCTGCAATTTTGCCACCTCTTAAAGATATATATCCAGGGAATCTCACGGATGATCTTCAAGGCTTGAGGGCTGATGCAGAAAAACGTATTGAAGAAACATGTGTTGATAACGTAACAAGGTTTAAGTCAGACAAATTTGGAGCAGAAAACAGAAGTTCATTGTCACAGCTTTTCATTTCATTCCTTGAGAAGTTTTCGGACCTTGTTCTGAAAGCAAGAAAGTTGGGAATTTGCCCATATACCGGACAGTGGGAAGACATACCAAGCAATATGACATGGTTGCCCCAAACATATACCATAATGATTGAAGATCCCTTTGATCAGCCAGAAAACTCTTCAAGGGCTGTTAGCATGGGAGAATTGACAAGGATATCTGAAGCTTTTAAGATGAGCAGCCACATACTCGTGTCAGCTAATCAGAGTGACATTCTTGCCACACTAGTCCGACCCCTAACCTACCGCCTTGTGACAGGAACATCTCACTACCAGTTTGAGCATCGCCATGGAGCTTATCCACAACGTTTCATGACGGAGAGCCCACATTCAAGAACACCAAGGGGGAATTATGTTCCCAGGCAACCACGTCGTCCTCAGGTGCATAAGTGGGCACAGAAACCCTCACAAGTCCAGCCTCAATTTCAGAAGATGAAGCCAGAAAACCATCCTGTCAAGCCCACTTTTCAAGAGATGAAGCCAGAAAACCATCCCAACAGGTCCACCCCTGATAACCCAACTCTAGAGACATCTCAGGATTGTGCATGCGGAATGTGTATGCCTGGGGAGTACATTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

414

Amino Acids

47.43

Weight (kDa)

8.83

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 55 - 102 3.6e-08 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 460
Acc36I ACCTGC 1 cut(s) 460
AccB7I CCANNNNNTGG 1 cut(s) 389
AciI CCGC 2 cut(s) 910, 1212
AclI AACGTT 1 cut(s) 970
AclWI GGATC 2 cut(s) 44, 755
AcsI RAATTY 3 cut(s) 185, 584, 678
AcuI CTGAAG 2 cut(s) 290, 846
AfaI GTAC 2 cut(s) 448, 1235
AfiI CCNNNNNNNGG 4 cut(s) 334, 389, 692, 699
AflIII ACRYGT 2 cut(s) 443, 548
AgsI TTSAA 9 cut(s) 79, 128, 400, 509, 542, 758, 789, 996, 1133
AhlI ACTAGT 1 cut(s) 889
AjiI CACGTC 1 cut(s) 1034
AjnI CCWGG 3 cut(s) 484, 1022, 1225
AluBI AGCT 4 cut(s) 619, 830, 863, 959
AluI AGCT 4 cut(s) 619, 830, 863, 959
Alw26I GTCTC 1 cut(s) 1184
AlwI GGATC 2 cut(s) 44, 755
ApeKI GCWGC 1 cut(s) 843
ApoI RAATTY 3 cut(s) 185, 584, 678
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 2 cut(s) 649, 1164
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 2 cut(s) 649, 1164
AxyI CCTNAGG 1 cut(s) 1041
BaeGI GKGCMC 1 cut(s) 1060
BanII GRGCYC 1 cut(s) 989
BauI CACGAG 1 cut(s) 854
BbsI GAAGAC 2 cut(s) 421, 711
BbvI GCAGC 1 cut(s) 855
BccI CCATC 5 cut(s) 13, 165, 311, 1119, 1161
BceAI ACGGC 1 cut(s) 120
BcgI CGANNNNNNTGC 2 cut(s) 1016, 1050
BciT130I CCWGG 3 cut(s) 486, 1024, 1227
BclI TGATCA 1 cut(s) 769
BcoDI GTCTC 1 cut(s) 1184
BcuI ACTAGT 1 cut(s) 889
BfaI CTAG 2 cut(s) 890, 1187
BfoI RGCGCY 1 cut(s) 325
BfuAI ACCTGC 1 cut(s) 460
BisI GCNGC 1 cut(s) 844
BlsI GCNGC 1 cut(s) 845
Bme1390I CCNGG 3 cut(s) 486, 1024, 1227
Bme18I GGWCC 2 cut(s) 649, 1164
BmgBI CACGTC 1 cut(s) 1034
BmgT120I GGNCC 2 cut(s) 649, 1164
BmiI GGNNCC 1 cut(s) 117
BmrFI CCNGG 3 cut(s) 486, 1024, 1227
BmsI GCATC 4 cut(s) 74, 116, 514, 955
BoxI GACNNNNGTC 1 cut(s) 391
BpiI GAAGAC 2 cut(s) 421, 711
BpuEI CTTGAG 2 cut(s) 535, 656
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 5 cut(s) 485, 952, 1004, 1022, 1226
BsaWI WCCGGW 1 cut(s) 692
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 389, 692, 699
Bse1I ACTGG 2 cut(s) 348, 937
Bse21I CCTNAGG 1 cut(s) 1041
Bse3DI GCAATG 1 cut(s) 68
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 3 cut(s) 486, 1024, 1227
BseDI CCNNGG 5 cut(s) 485, 952, 1004, 1022, 1226
BseGI GGATG 4 cut(s) 176, 505, 1111, 1153
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 4 cut(s) 334, 389, 692, 699
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 2 cut(s) 1055, 1211
BseNI ACTGG 2 cut(s) 348, 937
BseSI GKGCMC 1 cut(s) 1060
BseXI GCAGC 1 cut(s) 855
BsiSI CCGG 1 cut(s) 693
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 4 cut(s) 334, 389, 692, 699
BsmAI GTCTC 1 cut(s) 1184
BsmFI GGGAC 1 cut(s) 288
Bsp1286I GDGCHC 2 cut(s) 989, 1060
Bsp1407I TGTACA 1 cut(s) 446
Bsp143I GATC 4 cut(s) 49, 502, 760, 769
Bsp19I CCATGG 1 cut(s) 952
BspACI CCGC 2 cut(s) 910, 1212
BspCNI CTCAG 2 cut(s) 1054, 1210
BspHI TCATGA 1 cut(s) 975
BspLI GGNNCC 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 460
BspPI GGATC 2 cut(s) 44, 755
BsrDI GCAATG 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 446
BsrI ACTGG 2 cut(s) 348, 937
BssECI CCNNGG 5 cut(s) 485, 952, 1004, 1022, 1226
BssMI GATC 4 cut(s) 49, 502, 760, 769
BssSI CACGAG 1 cut(s) 854
BssT1I CCWWGG 2 cut(s) 952, 1004
Bst2BI CACGAG 1 cut(s) 854
Bst2UI CCWGG 3 cut(s) 486, 1024, 1227
Bst4CI ACNGT 2 cut(s) 149, 699
Bst6I CTCTTC 1 cut(s) 790
BstAUI TGTACA 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 1210
BstDEI CTNAG 3 cut(s) 140, 1041, 1197
BstDSI CCRYGG 1 cut(s) 952
BstF5I GGATG 4 cut(s) 176, 505, 1111, 1153
BstH2I RGCGCY 1 cut(s) 325
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 4 cut(s) 52, 505, 763, 772
BstMAI GTCTC 1 cut(s) 1184
BstMBI GATC 4 cut(s) 49, 502, 760, 769
BstNI CCWGG 3 cut(s) 486, 1024, 1227
BstNSI RCATGY 3 cut(s) 447, 552, 1212
BstPAI GACNNNNGTC 1 cut(s) 391
BstSCI CCNGG 3 cut(s) 484, 1022, 1225
BstSLI GKGCMC 1 cut(s) 1060
BstV1I GCAGC 1 cut(s) 855
BstV2I GAAGAC 2 cut(s) 421, 711
BstX2I RGATCY 1 cut(s) 760
BstYI RGATCY 1 cut(s) 760
Bsu36I CCTNAGG 1 cut(s) 1041
BtgI CCRYGG 1 cut(s) 952
BtgZI GCGATG 1 cut(s) 932
BtrI CACGTC 1 cut(s) 1034
BtsCI GGATG 4 cut(s) 176, 505, 1111, 1153
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 704
BveI ACCTGC 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 1210
CciI TCATGA 1 cut(s) 975
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 2 cut(s) 649, 1164
Csp6I GTAC 2 cut(s) 447, 1234
CviAII CATG 7 cut(s) 444, 549, 726, 802, 953, 976, 1209
CviQI GTAC 2 cut(s) 447, 1234
DdeI CTNAG 3 cut(s) 140, 1041, 1197
DpnI GATC 4 cut(s) 51, 504, 762, 771
DpnII GATC 4 cut(s) 49, 502, 760, 769
DraI TTTAAA 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 790
EarI CTCTTC 1 cut(s) 790
Eco130I CCWWGG 2 cut(s) 952, 1004
Eco24I GRGCYC 1 cut(s) 989
Eco32I GATATC 1 cut(s) 822
Eco47I GGWCC 2 cut(s) 649, 1164
Eco57I CTGAAG 2 cut(s) 290, 846
Eco81I CCTNAGG 1 cut(s) 1041
EcoRII CCWGG 3 cut(s) 484, 1022, 1225
EcoRV GATATC 1 cut(s) 822
EcoT14I CCWWGG 2 cut(s) 952, 1004
EcoT38I GRGCYC 1 cut(s) 989
ErhI CCWWGG 2 cut(s) 952, 1004
FaeI CATG 7 cut(s) 447, 552, 729, 805, 956, 979, 1212
FaqI GGGAC 1 cut(s) 288
FatI CATG 7 cut(s) 443, 548, 725, 801, 952, 975, 1208
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 1 cut(s) 844
FokI GGATG 4 cut(s) 183, 512, 1098, 1140
FriOI GRGCYC 1 cut(s) 989
Fsp4HI GCNGC 1 cut(s) 844
FspBI CTAG 2 cut(s) 890, 1187
GlaI GCGC 1 cut(s) 323
GluI GCNGC 1 cut(s) 844
HaeII RGCGCY 1 cut(s) 325
HapII CCGG 1 cut(s) 693
HhaI GCGC 1 cut(s) 324
Hin1II CATG 7 cut(s) 447, 552, 729, 805, 956, 979, 1212
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 828
HinfI GANTC 2 cut(s) 10, 490
HpaII CCGG 1 cut(s) 693
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 3 cut(s) 102, 449, 1167
Hpy188III TCNNGA 5 cut(s) 976, 996, 1133, 1187, 1199
Hpy8I GTNNAC 3 cut(s) 102, 449, 1167
Hpy99I CGWCG 1 cut(s) 1038
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 2 cut(s) 149, 699
HpyCH4IV ACGT 4 cut(s) 535, 561, 970, 1033
HpyCH4V TGCA 8 cut(s) 73, 87, 134, 200, 455, 527, 1048, 1208
HpyF3I CTNAG 3 cut(s) 140, 1041, 1197
HpySE526I ACGT 4 cut(s) 535, 561, 970, 1033
Hsp92II CATG 7 cut(s) 447, 552, 729, 805, 956, 979, 1212
HspAI GCGC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 4 cut(s) 49, 502, 760, 769
LmnI GCTCC 2 cut(s) 590, 956
Lsp1109I GCAGC 1 cut(s) 855
LweI GCATC 4 cut(s) 74, 116, 514, 955
MaeI CTAG 2 cut(s) 890, 1187
MaeII ACGT 4 cut(s) 535, 561, 970, 1033
MaeIII GTNAC 4 cut(s) 562, 612, 872, 916
MalI GATC 4 cut(s) 51, 504, 762, 771
MboI GATC 4 cut(s) 49, 502, 760, 769
MboII GAAGA 7 cut(s) 421, 497, 554, 716, 770, 777, 1105
MflI RGATCY 1 cut(s) 760
MhlI GDGCHC 2 cut(s) 989, 1060
MluCI AATT 8 cut(s) 129, 185, 456, 584, 678, 809, 1012, 1085
MlyI GAGTC 1 cut(s) 4
MmeI TCCRAC 1 cut(s) 919
MnlI CCTC 7 cut(s) 63, 422, 477, 510, 1050, 1078, 1092
MseI TTAA 7 cut(s) 96, 159, 183, 215, 471, 573, 834
MslI CAYNNNNRTG 3 cut(s) 38, 854, 951
MspI CCGG 1 cut(s) 693
MspR9I CCNGG 3 cut(s) 486, 1024, 1227
MvaI CCWGG 3 cut(s) 486, 1024, 1227
NcoI CCATGG 1 cut(s) 952
NdeII GATC 4 cut(s) 49, 502, 760, 769
NlaIII CATG 7 cut(s) 447, 552, 729, 805, 956, 979, 1212
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 3 cut(s) 612, 872, 916
NspI RCATGY 3 cut(s) 447, 552, 1212
PaeI GCATGC 1 cut(s) 1212
PagI TCATGA 1 cut(s) 975
PaqCI CACCTGC 1 cut(s) 460
PciI ACATGT 2 cut(s) 443, 548
PfeI GAWTC 1 cut(s) 490
PflMI CCANNNNNTGG 1 cut(s) 389
PkrI GCNGC 1 cut(s) 845
PleI GAGTC 1 cut(s) 4
PpsI GAGTC 1 cut(s) 4
PscI ACATGT 2 cut(s) 443, 548
PshAI GACNNNNGTC 1 cut(s) 391
Psp1406I AACGTT 1 cut(s) 970
Psp6I CCWGG 3 cut(s) 484, 1022, 1225
PspGI CCWGG 3 cut(s) 484, 1022, 1225
PspN4I GGNNCC 1 cut(s) 117
PspPI GGNCC 2 cut(s) 649, 1164
PsuI RGATCY 1 cut(s) 760
RsaI GTAC 2 cut(s) 448, 1235
RsaNI GTAC 2 cut(s) 447, 1234
RseI CAYNNNNRTG 3 cut(s) 38, 854, 951
SaqAI TTAA 7 cut(s) 96, 159, 183, 215, 471, 573, 834
SatI GCNGC 1 cut(s) 844
Sau3AI GATC 4 cut(s) 49, 502, 760, 769
Sau96I GGNCC 2 cut(s) 649, 1164
SchI GAGTC 1 cut(s) 4
ScrFI CCNGG 3 cut(s) 486, 1024, 1227
SduI GDGCHC 2 cut(s) 989, 1060
SfaNI GCATC 4 cut(s) 74, 116, 514, 955
SinI GGWCC 2 cut(s) 649, 1164
SmiMI CAYNNNNRTG 3 cut(s) 38, 854, 951
SmlI CTYRAG 2 cut(s) 514, 635
SmoI CTYRAG 2 cut(s) 514, 635
SpeI ACTAGT 1 cut(s) 889
SphI GCATGC 1 cut(s) 1212
Sse9I AATT 8 cut(s) 129, 185, 456, 584, 678, 809, 1012, 1085
SsiI CCGC 2 cut(s) 910, 1212
SspMI CTAG 2 cut(s) 890, 1187
StyD4I CCNGG 3 cut(s) 484, 1022, 1225
StyI CCWWGG 2 cut(s) 952, 1004
TaaI ACNGT 2 cut(s) 149, 699
TaiI ACGT 4 cut(s) 538, 564, 973, 1036
TaqI TCGA 1 cut(s) 222
TasI AATT 8 cut(s) 129, 185, 456, 584, 678, 809, 1012, 1085
TatI WGTACW 2 cut(s) 446, 1233
TfiI GAWTC 1 cut(s) 490
Tru1I TTAA 7 cut(s) 96, 159, 183, 215, 471, 573, 834
Tru9I TTAA 7 cut(s) 96, 159, 183, 215, 471, 573, 834
TscAI CASTG 2 cut(s) 202, 704
TseFI GTSAC 3 cut(s) 612, 872, 916
TseI GCWGC 1 cut(s) 843
Tsp45I GTSAC 3 cut(s) 612, 872, 916
TspDTI ATGAA 8 cut(s) 296, 389, 597, 613, 618, 964, 1112, 1154
TspGWI ACGGA 2 cut(s) 512, 995
TspRI CASTG 2 cut(s) 202, 704
Van91I CCANNNNNTGG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 649, 1164
XapI RAATTY 3 cut(s) 185, 584, 678
XbaI TCTAGA 1 cut(s) 1186
XceI RCATGY 3 cut(s) 447, 552, 1212
XspI CTAG 2 cut(s) 890, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.