Rw1G030940

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001926
Physical Location & Seq
Forward (+)
58947802 .. 58951133
3332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G030940.1

Sequence Viewer

Length: 1383 bp
ATGAATACACTTGTTATGTTGGATAATACTCTCAATCAGATTCTACGAGTTCTTAAACCCTCACTGGAAGATTGGGAAACACGACTGAGAATCCTTCATGAGTTACAAGGAGCTGTTGAATCTGTGGAGGGTTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTCGTGTCTAATCTGTATGCTTGCTGGGGAGACTTGGATATTTCAATTGAGTTTTCCAAAGGCTCATATATTTCAGAGTATGGGAAAAAGCATAAAGAACAATTACTAGGAGTTGTGATGAGAGCTACGAGACAGAGAGGTGGATGGAAAAGTTTTAAATTTATACCCACTGCAAGAGTTCCCATATTAAAGGTCGAAAGTTATCTCCAAAACATCTCTTGCGATATATCAATCAATAATCTGAAGGGACAAATGAAATCCAAACTCTTGTTTTGTATTGGTGAGATTGATGGGCGCTTCCGAGATATGGTTTTACTGGTAAAGGAATGGGCAAAAGCACAAAATATGAATAATCCAAGACTCGGGTCTTTCAACTCGTACGCCCTCACTTTGCTTGTGGTCTTCCACTTTCAGACATGTACACCTGCAATTTTGCCACCTCTTAAAGATATATATCCAGGGAATCTCACGGATGATCTTCAAGGCTTGAGGGCTGATGCAGAAAAACGTATTGAAGAAACATGTGTTGATAACGTAACAAGGTTTAAATCAGACAAATGTGGAGCAGAAAACAGAAGTTCATTGTCACAGCTTTTCATTTCATTCCTTGAGAAGTTTTCGGACCTTTTTCTGAAAACAAGAAAGTTGGGAATTTGCCCATATACCGGACAGTGGGAAGACATACCAAGCAATATGACATGGTTGCCCCAAACATATATGATAATGATTGAAGATCCCTTTGATCAGCCAGAAAACTCTTCAAGGGCTGTTAGCATGGGAGAATTGACAAGGATATCTGAAGCTTTTAAGATGAGCAGCCACATACTCATGTCAGCTAATCAGAGTGACATTCTAGCCACACTAGTCCGACCCCTAACCTACCGTCTTGTGACAGGAACATCTCACTACCAGTTTGAGCATCGTCATGGAGCTTATCCACACCGTTTCATGACTGAGAGCCCACATTCAAGAACACCAAGGGGGAATTATGTTCCCAGGCAACCACGTCGTCCTCAGGTGCATAAGTGGGCACAGAAACCATCACAAGTCCAGCCTCAATTTCAGAAGATGACGCCGGAAAACCATCCTGTCAAGCCCACTTTTCAAGAGATGAAGCCAGAAAACCATCCCAACAGGTCCACCCCTGATAACCCAACTCTAGAGACATCTCAGGATTGTGCATGCGGAATGTGTATGCCTAGGGAGTACATTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

460

Amino Acids

52.73

Weight (kDa)

8.68

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 7 - 148 1.3e-21 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 598
Acc36I ACCTGC 1 cut(s) 598
AciI CCGC 1 cut(s) 1350
AclWI GGATC 2 cut(s) 169, 893
AcsI RAATTY 2 cut(s) 323, 816
AcuI CTGAAG 2 cut(s) 428, 984
AcyI GRCGYC 1 cut(s) 1238
AfaI GTAC 3 cut(s) 545, 586, 1373
AfiI CCNNNNNNNGG 4 cut(s) 472, 527, 830, 837
AflIII ACRYGT 2 cut(s) 581, 686
AgsI TTSAA 9 cut(s) 119, 210, 538, 647, 680, 896, 927, 1134, 1271
AhlI ACTAGT 1 cut(s) 1027
AjiI CACGTC 1 cut(s) 1172
AjnI CCWGG 2 cut(s) 622, 1160
AluBI AGCT 6 cut(s) 113, 290, 757, 968, 1001, 1097
AluI AGCT 6 cut(s) 113, 290, 757, 968, 1001, 1097
Alw26I GTCTC 3 cut(s) 189, 289, 1322
AlwI GGATC 2 cut(s) 169, 893
Ama87I CYCGRG 1 cut(s) 527
ApeKI GCWGC 1 cut(s) 981
ApoI RAATTY 2 cut(s) 323, 816
AspA2I CCTAGG 1 cut(s) 1364
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 2 cut(s) 787, 1302
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 527
AvaII GGWCC 2 cut(s) 787, 1302
AvrII CCTAGG 1 cut(s) 1364
AxyI CCTNAGG 1 cut(s) 1179
BaeGI GKGCMC 1 cut(s) 1198
BanII GRGCYC 1 cut(s) 1127
BbsI GAAGAC 2 cut(s) 559, 849
BbvI GCAGC 1 cut(s) 993
BccI CCATC 5 cut(s) 303, 449, 1213, 1257, 1299
BcgI CGANNNNNNTGC 2 cut(s) 1154, 1188
BciT130I CCWGG 2 cut(s) 624, 1162
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 3 cut(s) 189, 289, 1322
BcuI ACTAGT 1 cut(s) 1027
BfaI CTAG 5 cut(s) 272, 1019, 1028, 1325, 1365
BfoI RGCGCY 1 cut(s) 463
BfuAI ACCTGC 1 cut(s) 598
BisI GCNGC 1 cut(s) 982
BlnI CCTAGG 1 cut(s) 1364
BlsI GCNGC 1 cut(s) 983
Bme1390I CCNGG 2 cut(s) 624, 1162
Bme18I GGWCC 2 cut(s) 787, 1302
BmeT110I CYCGRG 1 cut(s) 527
BmgBI CACGTC 1 cut(s) 1172
BmgT120I GGNCC 2 cut(s) 787, 1302
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 624, 1162
BmsI GCATC 2 cut(s) 652, 1093
BoxI GACNNNNGTC 1 cut(s) 529
BpiI GAAGAC 2 cut(s) 559, 849
BpuEI CTTGAG 2 cut(s) 673, 794
BsaBI GATNNNNATC 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 1238
BsaJI CCNNGG 4 cut(s) 623, 1142, 1160, 1364
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 4 cut(s) 472, 527, 830, 837
Bse1I ACTGG 3 cut(s) 69, 486, 1075
Bse21I CCTNAGG 1 cut(s) 1179
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 2 cut(s) 624, 1162
BseDI CCNNGG 4 cut(s) 623, 1142, 1160, 1364
BseGI GGATG 4 cut(s) 314, 643, 1249, 1291
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 4 cut(s) 472, 527, 830, 837
BseMII CTCAG 4 cut(s) 77, 1110, 1193, 1349
BseNI ACTGG 3 cut(s) 69, 486, 1075
BseSI GKGCMC 1 cut(s) 1198
BseXI GCAGC 1 cut(s) 993
BseYI CCCAGC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 2 cut(s) 831, 1241
BsiWI CGTACG 1 cut(s) 543
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 4 cut(s) 472, 527, 830, 837
BsmAI GTCTC 3 cut(s) 189, 289, 1322
BsmFI GGGAC 1 cut(s) 426
BsoBI CYCGRG 1 cut(s) 527
Bsp1286I GDGCHC 2 cut(s) 1127, 1198
Bsp1407I TGTACA 1 cut(s) 584
Bsp143I GATC 4 cut(s) 161, 640, 898, 907
BspACI CCGC 1 cut(s) 1350
BspCNI CTCAG 4 cut(s) 78, 1111, 1192, 1348
BspHI TCATGA 2 cut(s) 97, 1113
BspLI GGNNCC 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 2 cut(s) 169, 893
BsrGI TGTACA 1 cut(s) 584
BsrI ACTGG 3 cut(s) 69, 486, 1075
BssECI CCNNGG 4 cut(s) 623, 1142, 1160, 1364
BssMI GATC 4 cut(s) 161, 640, 898, 907
BssNI GRCGYC 1 cut(s) 1238
BssT1I CCWWGG 2 cut(s) 1142, 1364
Bst2UI CCWGG 2 cut(s) 624, 1162
Bst4CI ACNGT 4 cut(s) 148, 837, 1049, 1109
Bst6I CTCTTC 1 cut(s) 928
BstACI GRCGYC 1 cut(s) 1238
BstAUI TGTACA 1 cut(s) 584
BstC8I GCNNGC 2 cut(s) 187, 1348
BstDEI CTNAG 4 cut(s) 86, 1119, 1179, 1335
BstF5I GGATG 4 cut(s) 314, 643, 1249, 1291
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 4 cut(s) 164, 643, 901, 910
BstMAI GTCTC 3 cut(s) 189, 289, 1322
BstMBI GATC 4 cut(s) 161, 640, 898, 907
BstNI CCWGG 2 cut(s) 624, 1162
BstNSI RCATGY 3 cut(s) 585, 690, 1350
BstPAI GACNNNNGTC 1 cut(s) 529
BstSCI CCNGG 2 cut(s) 622, 1160
BstSLI GKGCMC 1 cut(s) 1198
BstV1I GCAGC 1 cut(s) 993
BstV2I GAAGAC 2 cut(s) 559, 849
BstX2I RGATCY 1 cut(s) 898
BstYI RGATCY 1 cut(s) 898
Bsu36I CCTNAGG 1 cut(s) 1179
BtrI CACGTC 1 cut(s) 1172
BtsCI GGATG 4 cut(s) 314, 643, 1249, 1291
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 3 cut(s) 62, 333, 842
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 187, 1348
CciI TCATGA 2 cut(s) 97, 1113
CfoI GCGC 1 cut(s) 462
Cfr13I GGNCC 2 cut(s) 787, 1302
CseI GACGC 1 cut(s) 1246
Csp6I GTAC 3 cut(s) 544, 585, 1372
CviAII CATG 9 cut(s) 98, 582, 687, 864, 940, 994, 1091, 1114, 1347
CviQI GTAC 3 cut(s) 544, 585, 1372
DdeI CTNAG 4 cut(s) 86, 1119, 1179, 1335
DpnI GATC 4 cut(s) 163, 642, 900, 909
DpnII GATC 4 cut(s) 161, 640, 898, 907
DraI TTTAAA 2 cut(s) 322, 712
Eam1104I CTCTTC 1 cut(s) 928
EarI CTCTTC 1 cut(s) 928
Eco130I CCWWGG 2 cut(s) 1142, 1364
Eco24I GRGCYC 1 cut(s) 1127
Eco32I GATATC 1 cut(s) 960
Eco47I GGWCC 2 cut(s) 787, 1302
Eco57I CTGAAG 2 cut(s) 428, 984
Eco81I CCTNAGG 1 cut(s) 1179
Eco88I CYCGRG 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 622, 1160
EcoRV GATATC 1 cut(s) 960
EcoT14I CCWWGG 2 cut(s) 1142, 1364
EcoT38I GRGCYC 1 cut(s) 1127
ErhI CCWWGG 2 cut(s) 1142, 1364
FaeI CATG 9 cut(s) 101, 585, 690, 867, 943, 997, 1094, 1117, 1350
FaqI GGGAC 1 cut(s) 426
FatI CATG 9 cut(s) 97, 581, 686, 863, 939, 993, 1090, 1113, 1346
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 1 cut(s) 982
FokI GGATG 4 cut(s) 321, 650, 1236, 1278
FriOI GRGCYC 1 cut(s) 1127
Fsp4HI GCNGC 1 cut(s) 982
FspBI CTAG 5 cut(s) 272, 1019, 1028, 1325, 1365
GlaI GCGC 1 cut(s) 461
GluI GCNGC 1 cut(s) 982
GsaI CCCAGC 1 cut(s) 193
HaeII RGCGCY 1 cut(s) 463
HapII CCGG 2 cut(s) 831, 1241
HgaI GACGC 1 cut(s) 1246
HhaI GCGC 1 cut(s) 462
Hin1I GRCGYC 1 cut(s) 1238
Hin1II CATG 9 cut(s) 101, 585, 690, 867, 943, 997, 1094, 1117, 1350
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 966
HinfI GANTC 5 cut(s) 40, 90, 119, 525, 628
HpaII CCGG 2 cut(s) 831, 1241
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 2 cut(s) 587, 1305
Hpy188III TCNNGA 6 cut(s) 98, 1114, 1134, 1271, 1325, 1337
Hpy8I GTNNAC 2 cut(s) 587, 1305
Hpy99I CGWCG 1 cut(s) 1176
HpyAV CCTTC 2 cut(s) 104, 403
HpyCH4III ACNGT 4 cut(s) 148, 837, 1049, 1109
HpyCH4IV ACGT 3 cut(s) 673, 699, 1171
HpyCH4V TGCA 6 cut(s) 143, 338, 593, 665, 1186, 1346
HpyF3I CTNAG 4 cut(s) 86, 1119, 1179, 1335
HpySE526I ACGT 3 cut(s) 673, 699, 1171
Hsp92I GRCGYC 1 cut(s) 1238
Hsp92II CATG 9 cut(s) 101, 585, 690, 867, 943, 997, 1094, 1117, 1350
HspAI GCGC 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 4 cut(s) 161, 640, 898, 907
LmnI GCTCC 4 cut(s) 110, 151, 728, 1094
Lsp1109I GCAGC 1 cut(s) 993
LweI GCATC 2 cut(s) 652, 1093
MaeI CTAG 5 cut(s) 272, 1019, 1028, 1325, 1365
MaeII ACGT 3 cut(s) 673, 699, 1171
MaeIII GTNAC 5 cut(s) 102, 700, 750, 1010, 1054
MalI GATC 4 cut(s) 163, 642, 900, 909
MboI GATC 4 cut(s) 161, 640, 898, 907
MboII GAAGA 8 cut(s) 80, 559, 635, 692, 854, 908, 915, 1243
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 898
MhlI GDGCHC 2 cut(s) 1127, 1198
MluCI AATT 8 cut(s) 210, 266, 323, 594, 816, 947, 1150, 1223
MlyI GAGTC 1 cut(s) 519
MmeI TCCRAC 1 cut(s) 1057
MnlI CCTC 9 cut(s) 70, 121, 131, 296, 560, 615, 648, 1188, 1230
MseI TTAA 7 cut(s) 54, 134, 321, 353, 609, 711, 972
MslI CAYNNNNRTG 2 cut(s) 992, 1089
MspI CCGG 2 cut(s) 831, 1241
MspR9I CCNGG 2 cut(s) 624, 1162
MunI CAATTG 1 cut(s) 210
MvaI CCWGG 2 cut(s) 624, 1162
NdeII GATC 4 cut(s) 161, 640, 898, 907
NlaIII CATG 9 cut(s) 101, 585, 690, 867, 943, 997, 1094, 1117, 1350
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 3 cut(s) 750, 1010, 1054
NspI RCATGY 3 cut(s) 585, 690, 1350
PaeI GCATGC 1 cut(s) 1350
PagI TCATGA 2 cut(s) 97, 1113
PaqCI CACCTGC 1 cut(s) 598
PciI ACATGT 2 cut(s) 581, 686
PfeI GAWTC 4 cut(s) 40, 90, 119, 628
Pfl23II CGTACG 1 cut(s) 543
PkrI GCNGC 1 cut(s) 983
PleI GAGTC 1 cut(s) 519
PpsI GAGTC 1 cut(s) 519
PscI ACATGT 2 cut(s) 581, 686
PshAI GACNNNNGTC 1 cut(s) 529
Psp6I CCWGG 2 cut(s) 622, 1160
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 2 cut(s) 622, 1160
PspLI CGTACG 1 cut(s) 543
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 787, 1302
PsuI RGATCY 1 cut(s) 898
RsaI GTAC 3 cut(s) 545, 586, 1373
RsaNI GTAC 3 cut(s) 544, 585, 1372
RseI CAYNNNNRTG 2 cut(s) 992, 1089
SaqAI TTAA 7 cut(s) 54, 134, 321, 353, 609, 711, 972
SatI GCNGC 1 cut(s) 982
Sau3AI GATC 4 cut(s) 161, 640, 898, 907
Sau96I GGNCC 2 cut(s) 787, 1302
SchI GAGTC 1 cut(s) 519
ScrFI CCNGG 2 cut(s) 624, 1162
SduI GDGCHC 2 cut(s) 1127, 1198
SfaNI GCATC 2 cut(s) 652, 1093
SinI GGWCC 2 cut(s) 787, 1302
SmiMI CAYNNNNRTG 2 cut(s) 992, 1089
SmlI CTYRAG 2 cut(s) 652, 773
SmoI CTYRAG 2 cut(s) 652, 773
SpeI ACTAGT 1 cut(s) 1027
SphI GCATGC 1 cut(s) 1350
Sse9I AATT 8 cut(s) 210, 266, 323, 594, 816, 947, 1150, 1223
SsiI CCGC 1 cut(s) 1350
SspMI CTAG 5 cut(s) 272, 1019, 1028, 1325, 1365
StyD4I CCNGG 2 cut(s) 622, 1160
StyI CCWWGG 2 cut(s) 1142, 1364
TaaI ACNGT 4 cut(s) 148, 837, 1049, 1109
TaiI ACGT 3 cut(s) 676, 702, 1174
TaqI TCGA 1 cut(s) 360
TasI AATT 8 cut(s) 210, 266, 323, 594, 816, 947, 1150, 1223
TatI WGTACW 2 cut(s) 584, 1371
TfiI GAWTC 4 cut(s) 40, 90, 119, 628
Tru1I TTAA 7 cut(s) 54, 134, 321, 353, 609, 711, 972
Tru9I TTAA 7 cut(s) 54, 134, 321, 353, 609, 711, 972
TscAI CASTG 3 cut(s) 69, 340, 842
TseFI GTSAC 3 cut(s) 750, 1010, 1054
TseI GCWGC 1 cut(s) 981
Tsp45I GTSAC 3 cut(s) 750, 1010, 1054
TspDTI ATGAA 9 cut(s) 17, 86, 434, 527, 735, 751, 756, 1102, 1292
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 3 cut(s) 69, 340, 842
VpaK11BI GGWCC 2 cut(s) 787, 1302
XapI RAATTY 2 cut(s) 323, 816
XbaI TCTAGA 1 cut(s) 1324
XceI RCATGY 3 cut(s) 585, 690, 1350
XmaJI CCTAGG 1 cut(s) 1364
XspI CTAG 5 cut(s) 272, 1019, 1028, 1325, 1365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.