Rorug01G0340300

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001926
Physical Location & Seq
Forward (+)
45831937 .. 45832526
590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0340300.1

Sequence Viewer

Length: 513 bp
ATGGAGAAGGGAAGACTTCAGGTTTCTCGATCAACTCTTACCCCACTGCTCCAAAGATCAGAAGATGTAGACCTGACTCCGATCACCAATAAGCTGTGGAAGAGGTTCTTCGAGAGAGACTTCGGTGGCAAAGCCACTGATGAGGTGATCCAGAAGATGAAGATCAAGAAAATGAGTTTTAAGTGGTCGGAGTTGTACGAGGCCAAGTCAAAGAGGATAGAAGAGGATGAGAAAGAAGTAGGTGAAAGGTTAAAGAAGCTGTATGAGAAAGAAGCTGCTCGGAAACAAAGCCGGCAAGTTAAGGTGTTGGACAAGGTTCCACCTTCTTCGTCAAGCAACAAAACAACTGGGTCCAAGAAAGAGAGCAAACTGATGAGAAAATTAAGGAAACAGCATCTGAATTATCTGGAAGTGAGAAACAAAGAAGCTATGAAGATGAAGAGAACTGCTGCCACCTGTTCTGGTCTCATCAGAAAGCCAAGAACGAGTATTAAACCTATGAACGTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

170

Amino Acids

19.94

Weight (kDa)

10.21

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elongin_A PF06881 15 - 99 1.2e-12 RNA polymerase II transcription factor SIII (Elongin) subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 69
AclWI GGATC 1 cut(s) 142
AfaI GTAC 1 cut(s) 197
AluBI AGCT 4 cut(s) 94, 259, 275, 428
AluI AGCT 4 cut(s) 94, 259, 275, 428
Alw26I GTCTC 2 cut(s) 111, 470
AlwI GGATC 1 cut(s) 142
AlwNI CAGNNNCTG 1 cut(s) 397
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 2 cut(s) 275, 449
Asp700I GAANNNNTTC 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 351
AsuHPI GGTGA 3 cut(s) 76, 157, 254
AvaII GGWCC 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 262, 436
BcoDI GTCTC 2 cut(s) 111, 470
BisI GCNGC 2 cut(s) 276, 450
BlsI GCNGC 2 cut(s) 277, 451
Bme18I GGWCC 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 351
BmiI GGNNCC 2 cut(s) 318, 352
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 1 cut(s) 403
BmuI ACTGGG 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 19
BsaBI GATNNNNATC 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 1 cut(s) 352
Bse8I GATNNNNATC 1 cut(s) 161
BseGI GGATG 1 cut(s) 232
BseJI GATNNNNATC 1 cut(s) 161
BseNI ACTGG 1 cut(s) 352
BseXI GCAGC 2 cut(s) 262, 436
BshFI GGCC 1 cut(s) 203
BsiSI CCGG 1 cut(s) 292
BsmAI GTCTC 2 cut(s) 111, 470
BsnI GGCC 1 cut(s) 203
Bso31I GGTCTC 1 cut(s) 470
Bsp143I GATC 5 cut(s) 29, 56, 81, 147, 162
BspANI GGCC 1 cut(s) 203
BspLI GGNNCC 2 cut(s) 318, 352
BspPI GGATC 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 291
BsrI ACTGG 1 cut(s) 352
BssAI RCCGGY 1 cut(s) 291
BssMI GATC 5 cut(s) 29, 56, 81, 147, 162
Bst6I CTCTTC 3 cut(s) 95, 216, 434
BstC8I GCNNGC 1 cut(s) 293
BstF5I GGATG 1 cut(s) 232
BstKTI GATC 5 cut(s) 32, 59, 84, 150, 165
BstMAI GTCTC 2 cut(s) 111, 470
BstMBI GATC 5 cut(s) 29, 56, 81, 147, 162
BstV1I GCAGC 2 cut(s) 262, 436
BstV2I GAAGAC 1 cut(s) 19
BsuRI GGCC 1 cut(s) 203
BtsCI GGATG 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 2 cut(s) 44, 135
Cac8I GCNNGC 1 cut(s) 293
CaiI CAGNNNCTG 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 351
Csp6I GTAC 1 cut(s) 196
CviJI RGCY 8 cut(s) 94, 134, 203, 259, 275, 291, 428, 478
CviKI_1 RGCY 8 cut(s) 94, 134, 203, 259, 275, 291, 428, 478
CviQI GTAC 1 cut(s) 196
DpnI GATC 5 cut(s) 31, 58, 83, 149, 164
DpnII GATC 5 cut(s) 29, 56, 81, 147, 162
Eam1104I CTCTTC 3 cut(s) 95, 216, 434
EarI CTCTTC 3 cut(s) 95, 216, 434
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 351
FaiI YATR 3 cut(s) 264, 431, 500
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 2 cut(s) 276, 450
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 2 cut(s) 276, 450
GluI GCNGC 2 cut(s) 276, 450
HaeIII GGCC 1 cut(s) 203
HapII CCGG 1 cut(s) 292
HinfI GANTC 1 cut(s) 76
HpaII CCGG 1 cut(s) 292
HphI GGTGA 3 cut(s) 76, 157, 254
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 6 cut(s) 61, 81, 190, 282, 399, 473
Hpy188III TCNNGA 5 cut(s) 27, 112, 151, 166, 407
Hpy8I GTNNAC 1 cut(s) 70
HpyAV CCTTC 1 cut(s) 333
HpyCH4IV ACGT 1 cut(s) 504
HpySE526I ACGT 1 cut(s) 504
KroI GCCGGC 1 cut(s) 291
KroNI GCCGGC 1 cut(s) 293
Kzo9I GATC 5 cut(s) 29, 56, 81, 147, 162
LmnI GCTCC 1 cut(s) 54
LpnPI CCDG 8 cut(s) 5, 86, 164, 305, 333, 392, 447, 469
Lsp1109I GCAGC 2 cut(s) 262, 436
LweI GCATC 1 cut(s) 403
MaeII ACGT 1 cut(s) 504
MalI GATC 5 cut(s) 31, 58, 83, 149, 164
MboI GATC 5 cut(s) 29, 56, 81, 147, 162
MluCI AATT 2 cut(s) 380, 400
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 2 cut(s) 168, 288
MnlI CCTC 5 cut(s) 96, 136, 193, 207, 217
MroNI GCCGGC 1 cut(s) 291
MroXI GAANNNNTTC 1 cut(s) 104
MseI TTAA 5 cut(s) 180, 251, 300, 383, 492
MspI CCGG 1 cut(s) 292
NaeI GCCGGC 1 cut(s) 293
NdeII GATC 5 cut(s) 29, 56, 81, 147, 162
NgoMIV GCCGGC 1 cut(s) 291
NlaIV GGNNCC 2 cut(s) 318, 352
PdiI GCCGGC 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 104
PkrI GCNGC 2 cut(s) 277, 451
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 318, 352
PspPI GGNCC 1 cut(s) 351
PstNI CAGNNNCTG 1 cut(s) 397
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SaqAI TTAA 5 cut(s) 180, 251, 300, 383, 492
SatI GCNGC 2 cut(s) 276, 450
Sau3AI GATC 5 cut(s) 29, 56, 81, 147, 162
Sau96I GGNCC 1 cut(s) 351
SchI GAGTC 1 cut(s) 70
SfaNI GCATC 1 cut(s) 403
SinI GGWCC 1 cut(s) 351
Sse9I AATT 2 cut(s) 380, 400
TaiI ACGT 1 cut(s) 507
TaqI TCGA 2 cut(s) 28, 111
TasI AATT 2 cut(s) 380, 400
Tru1I TTAA 5 cut(s) 180, 251, 300, 383, 492
Tru9I TTAA 5 cut(s) 180, 251, 300, 383, 492
TscAI CASTG 2 cut(s) 51, 142
TseI GCWGC 2 cut(s) 275, 449
TspDTI ATGAA 3 cut(s) 173, 446, 452
TspRI CASTG 2 cut(s) 51, 142
VpaK11BI GGWCC 1 cut(s) 351
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.