Rroxscaffold_4G00288650

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001926
Physical Location & Seq
Reverse (-)
9343201 .. 9348043
4843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00288650.1

Sequence Viewer

Length: 1320 bp
ATGGAGAATACTCTCAGGGAGATAATACGTGTGCTTGAACCCTTGCCCACTGATCGAGAAACACGATGCCAAATTCTTCGGGAGTTACAAGGAGCTGTTGAGTCTGTAGAAAGCTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTTGTCTCAGAACTTTTTACTCGCTGGGGAGACTTGGATGTTTCAATTGAGTTCTCTAAAGGCGCATATATTTCTTCTTATGGAAGAAAGCACACACAAGATGTACTAAAAGATTTAATGAAGGCTATGAGAAAGAGAGGTGGATGGAACAGGTATCAGTTCATACCTAATGCAAGAGTTCCCATTCTGATGGTCAAAAGTAATCTTGGAAACATCTCCTGTGATATTTCAGTCAATAACCTGAAAGCCCAAATGAAATCAAAACTCTTTTTATGGATCAATGATATAGATCCACGCTTTCGTGATATGGTTTTACTGGTGAAGGAATGGGCAAAAGCACACAATATCAATAATTCAAAATCTGGGACTTTCAATTCATACTCTCTGAGTTTGCTTGTGATCTTCCATTTTCAGACATGTACACCTGCAATTCTGCCTCCTCTTCAAGACATATATACAGAAACTCTCACGGATGATCTTAAAGGCTTGAGGGATGATGCAGAAAGACGTATTGAAAAAAGTTGTGTTGAGAACATGAGAAGATTTAAACTAAACAAATTGAGAGCTCGGAATAAAAGTTCTTTGTTTGAGCTTTTCATTTCATTCCTTGGGAAGTTCTCTGACCTTGGTTCGAAGGCTGCGGAGTTGGGAATTTGCACATATTCTGGAAAATGGGAAAGAATAGAAAGCAATATGGGATGGTTGCCCAAAACCTATTGTATATTGGTTGAAGATCCTTTTGATCGGCCAGAAAATGCTGCAAGAGGTGTCGGCCAAAAAGCATTGAAAAGGATCTCTGAAGTTTTTGAGATGAGTCGTGACAGGCTAATCGCAGTTCGTTACAATGCCTCCTTAGCGATTGAGAGTTCCCTTCTTGCCACACTAGTCCGACCACATACGCTACATCTTATTAGCAACGCTCCAGGTGGTTACAATATTAAGCATCACCAAACAACTCAACCACAATATTTCAGGGCTGAGAGCTCACATTCACGAACCCGAAGACATTATAACGGTCCCCCGAAACCAAATGGTCCTTTTGTACGAGAGTTGGTGCCCCCTAGGGAATATAACGGCCCCCCAAAACCAAATGGTAAGGTACGAGAGTTGGTGCCCCAAAGGGATTATAATGGCCCCCGAAACCAAGTGGCCCTTATGGACGAGAGTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

439

Amino Acids

50.3

Weight (kDa)

9.58

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 3 - 139 1.9e-21 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1158, 1275
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 2 cut(s) 1201, 1258
AciI CCGC 1 cut(s) 788
AclWI GGATC 5 cut(s) 151, 431, 431, 875, 947
AcoI YGGCCR 2 cut(s) 893, 919
AcsI RAATTY 2 cut(s) 72, 798
AcuI CTGAAG 1 cut(s) 966
AfaI GTAC 4 cut(s) 252, 568, 1191, 1248
AflII CTTAAG 1 cut(s) 115
AflIII ACRYGT 2 cut(s) 28, 563
AgsI TTSAA 8 cut(s) 38, 192, 504, 520, 593, 662, 878, 934
AhlI ACTAGT 1 cut(s) 1030
AjnI CCWGG 1 cut(s) 1069
AloI GAACNNNNNNTCC 2 cut(s) 709, 741
AluBI AGCT 5 cut(s) 95, 114, 713, 739, 1131
AluI AGCT 5 cut(s) 95, 114, 713, 739, 1131
Alw21I GWGCWC 2 cut(s) 715, 1133
Alw26I GTCTC 2 cut(s) 157, 171
AlwI GGATC 5 cut(s) 151, 431, 431, 875, 947
AoxI GGCC 5 cut(s) 893, 919, 1222, 1279, 1296
ApeKI GCWGC 2 cut(s) 785, 905
ApoI RAATTY 2 cut(s) 72, 798
AspA2I CCTAGG 1 cut(s) 1208
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 5 cut(s) 1163, 1181, 1223, 1280, 1297
AsuHPI GGTGA 2 cut(s) 478, 1085
AsuII TTCGAA 1 cut(s) 779
AvaII GGWCC 2 cut(s) 1163, 1181
AvrII CCTAGG 1 cut(s) 1208
BaeGI GKGCMC 2 cut(s) 1206, 1263
BaeI ACNNNNGTAYC 2 cut(s) 1238, 1271
BanI GGYRCC 2 cut(s) 1201, 1258
BanII GRGCYC 2 cut(s) 715, 1133
BbsI GAAGAC 1 cut(s) 1156
Bbv12I GWGCWC 2 cut(s) 715, 1133
BbvI GCAGC 2 cut(s) 772, 892
BccI CCATC 3 cut(s) 285, 331, 840
BceAI ACGGC 1 cut(s) 1237
BciT130I CCWGG 1 cut(s) 1071
BcoDI GTCTC 2 cut(s) 157, 171
BcuI ACTAGT 1 cut(s) 1030
BfaI CTAG 2 cut(s) 1031, 1209
BfmI CTRYAG 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 2 cut(s) 786, 906
BlnI CCTAGG 1 cut(s) 1208
BlsI GCNGC 2 cut(s) 787, 907
Bme1390I CCNGG 1 cut(s) 1071
Bme18I GGWCC 2 cut(s) 1163, 1181
BmgT120I GGNCC 5 cut(s) 1163, 1181, 1223, 1280, 1297
BmiI GGNNCC 6 cut(s) 135, 1165, 1203, 1225, 1260, 1282
BmrFI CCNGG 1 cut(s) 1071
BmsI GCATC 3 cut(s) 56, 634, 1099
BoxI GACNNNNGTC 1 cut(s) 1311
BpiI GAAGAC 1 cut(s) 1156
BpmI CTGGAG 1 cut(s) 1053
Bpu10I CCTNAGC 1 cut(s) 1000
Bpu14I TTCGAA 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 655
BsaAI YACGTR 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 3 cut(s) 754, 772, 1208
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bse1I ACTGG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 1 cut(s) 1071
BseDI CCNNGG 3 cut(s) 754, 772, 1208
BseGI GGATG 5 cut(s) 190, 296, 625, 646, 851
BseJI GATNNNNATC 1 cut(s) 435
BseMII CTCAG 4 cut(s) 28, 168, 524, 1116
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 2 cut(s) 1206, 1263
BseXI GCAGC 2 cut(s) 772, 892
BseYI CCCAGC 1 cut(s) 171
BshFI GGCC 5 cut(s) 895, 921, 1224, 1281, 1298
BshNI GGYRCC 2 cut(s) 1201, 1258
BsiHKAI GWGCWC 2 cut(s) 715, 1133
BslFI GGGAC 2 cut(s) 526, 1149
BsmAI GTCTC 2 cut(s) 157, 171
BsmFI GGGAC 2 cut(s) 526, 1149
BsnI GGCC 5 cut(s) 895, 921, 1224, 1281, 1298
Bsp119I TTCGAA 1 cut(s) 779
Bsp1286I GDGCHC 4 cut(s) 715, 1133, 1206, 1263
Bsp1407I TGTACA 1 cut(s) 566
Bsp143I GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
BspACI CCGC 1 cut(s) 788
BspANI GGCC 5 cut(s) 895, 921, 1224, 1281, 1298
BspCNI CTCAG 4 cut(s) 27, 167, 525, 1117
BspLI GGNNCC 6 cut(s) 135, 1165, 1203, 1225, 1260, 1282
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 5 cut(s) 151, 431, 431, 875, 947
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 2 cut(s) 1201, 1258
BspTI CTTAAG 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 3 cut(s) 754, 772, 1208
BssMI GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
BssT1I CCWWGG 3 cut(s) 754, 772, 1208
Bst2UI CCWGG 1 cut(s) 1071
Bst4CI ACNGT 2 cut(s) 130, 1163
Bst6I CTCTTC 1 cut(s) 594
BstAFI CTTAAG 1 cut(s) 115
BstAUI TGTACA 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 779
BstDEI CTNAG 5 cut(s) 14, 154, 533, 1000, 1125
BstF5I GGATG 5 cut(s) 190, 296, 625, 646, 851
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 9 cut(s) 55, 146, 426, 439, 549, 625, 883, 892, 942
BstMAI GTCTC 2 cut(s) 157, 171
BstMBI GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 1 cut(s) 1071
BstNSI RCATGY 1 cut(s) 567
BstPAI GACNNNNGTC 1 cut(s) 1311
BstSCI CCNGG 1 cut(s) 1069
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 2 cut(s) 1206, 1263
BstV1I GCAGC 2 cut(s) 772, 892
BstV2I GAAGAC 1 cut(s) 1156
BstX2I RGATCY 3 cut(s) 436, 880, 939
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 436, 880, 939
BsuRI GGCC 5 cut(s) 895, 921, 1224, 1281, 1298
BtsCI GGATG 5 cut(s) 190, 296, 625, 646, 851
BtsIMutI CAGTG 1 cut(s) 48
BveI ACCTGC 1 cut(s) 580
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 5 cut(s) 1163, 1181, 1223, 1280, 1297
Csp6I GTAC 4 cut(s) 251, 567, 1190, 1247
CviAII CATG 2 cut(s) 564, 682
CviQI GTAC 4 cut(s) 251, 567, 1190, 1247
DdeI CTNAG 5 cut(s) 14, 154, 533, 1000, 1125
DpnI GATC 9 cut(s) 54, 145, 425, 438, 548, 624, 882, 891, 941
DpnII GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 2 cut(s) 893, 919
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 2 cut(s) 713, 1131
Eco130I CCWWGG 3 cut(s) 754, 772, 1208
Eco24I GRGCYC 2 cut(s) 715, 1133
Eco47I GGWCC 2 cut(s) 1163, 1181
Eco53kI GAGCTC 2 cut(s) 713, 1131
Eco57I CTGAAG 1 cut(s) 966
EcoICRI GAGCTC 2 cut(s) 713, 1131
EcoRII CCWGG 1 cut(s) 1069
EcoT14I CCWWGG 3 cut(s) 754, 772, 1208
EcoT38I GRGCYC 2 cut(s) 715, 1133
ErhI CCWWGG 3 cut(s) 754, 772, 1208
FaeI CATG 2 cut(s) 567, 685
FalI AAGNNNNNCTT 2 cut(s) 1284, 1316
FaqI GGGAC 2 cut(s) 526, 1149
FatI CATG 2 cut(s) 563, 681
Fnu4HI GCNGC 2 cut(s) 786, 906
FokI GGATG 5 cut(s) 197, 303, 632, 653, 858
FriOI GRGCYC 2 cut(s) 715, 1133
Fsp4HI GCNGC 2 cut(s) 786, 906
FspBI CTAG 2 cut(s) 1031, 1209
GlaI GCGC 1 cut(s) 211
GluI GCNGC 2 cut(s) 786, 906
GsaI CCCAGC 1 cut(s) 175
GsuI CTGGAG 1 cut(s) 1053
HaeIII GGCC 5 cut(s) 895, 921, 1224, 1281, 1298
HhaI GCGC 1 cut(s) 212
Hin1II CATG 2 cut(s) 567, 685
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 3 cut(s) 101, 961, 1312
HphI GGTGA 2 cut(s) 478, 1085
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 8 cut(s) 157, 336, 534, 561, 717, 769, 946, 1037
Hpy188III TCNNGA 7 cut(s) 56, 80, 449, 593, 813, 965, 1140
Hpy8I GTNNAC 1 cut(s) 569
HpyAV CCTTC 4 cut(s) 262, 463, 775, 1028
HpyCH4III ACNGT 2 cut(s) 130, 1163
HpyCH4IV ACGT 2 cut(s) 28, 655
HpyCH4V TGCA 6 cut(s) 125, 320, 575, 647, 804, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 5 cut(s) 14, 154, 533, 1000, 1125
HpySE526I ACGT 2 cut(s) 28, 655
Hsp92II CATG 2 cut(s) 567, 685
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
LmnI GCTCC 3 cut(s) 92, 133, 1072
Lsp1109I GCAGC 2 cut(s) 772, 892
LweI GCATC 3 cut(s) 56, 634, 1099
MaeI CTAG 2 cut(s) 1031, 1209
MaeII ACGT 2 cut(s) 28, 655
MaeIII GTNAC 4 cut(s) 84, 965, 986, 1076
MalI GATC 9 cut(s) 54, 145, 425, 438, 548, 624, 882, 891, 941
MboI GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
MboII GAAGA 8 cut(s) 68, 213, 243, 541, 581, 699, 890, 1161
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 3 cut(s) 436, 880, 939
MhlI GDGCHC 4 cut(s) 715, 1133, 1206, 1263
MluCI AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
MlyI GAGTC 2 cut(s) 110, 970
MmeI TCCRAC 1 cut(s) 1060
MnlI CCTC 7 cut(s) 113, 278, 594, 597, 630, 905, 1006
MseI TTAA 5 cut(s) 116, 263, 627, 693, 1086
MslI CAYNNNNRTG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 115
MspR9I CCNGG 1 cut(s) 1071
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 1071
MwoI GCNNNNNNNGC 1 cut(s) 1001
NdeII GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
NlaIII CATG 2 cut(s) 567, 685
NlaIV GGNNCC 6 cut(s) 135, 1165, 1203, 1225, 1260, 1282
NmuCI GTSAC 1 cut(s) 965
NspI RCATGY 1 cut(s) 567
NspV TTCGAA 1 cut(s) 779
PaqCI CACCTGC 1 cut(s) 580
PciI ACATGT 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PkrI GCNGC 2 cut(s) 787, 907
PleI GAGTC 2 cut(s) 109, 969
PpsI GAGTC 2 cut(s) 109, 969
Ppu21I YACGTR 1 cut(s) 29
PscI ACATGT 1 cut(s) 563
PshAI GACNNNNGTC 1 cut(s) 1311
PsiI TTATAA 2 cut(s) 1158, 1275
Psp124BI GAGCTC 2 cut(s) 715, 1133
Psp6I CCWGG 1 cut(s) 1069
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 1 cut(s) 1069
PspN4I GGNNCC 6 cut(s) 135, 1165, 1203, 1225, 1260, 1282
PspPI GGNCC 5 cut(s) 1163, 1181, 1223, 1280, 1297
PsuI RGATCY 3 cut(s) 436, 880, 939
RsaI GTAC 4 cut(s) 252, 568, 1191, 1248
RsaNI GTAC 4 cut(s) 251, 567, 1190, 1247
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 715, 1133
SaqAI TTAA 5 cut(s) 116, 263, 627, 693, 1086
SatI GCNGC 2 cut(s) 786, 906
Sau3AI GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
Sau96I GGNCC 5 cut(s) 1163, 1181, 1223, 1280, 1297
SchI GAGTC 2 cut(s) 110, 970
ScrFI CCNGG 1 cut(s) 1071
SduI GDGCHC 4 cut(s) 715, 1133, 1206, 1263
SfaNI GCATC 3 cut(s) 56, 634, 1099
SfcI CTRYAG 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 2 cut(s) 1163, 1181
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 115, 634
SmoI CTYRAG 2 cut(s) 115, 634
SpeI ACTAGT 1 cut(s) 1030
Sse9I AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
SsiI CCGC 1 cut(s) 788
SspI AATATT 2 cut(s) 1084, 1115
SspMI CTAG 2 cut(s) 1031, 1209
SstI GAGCTC 2 cut(s) 715, 1133
StyD4I CCNGG 1 cut(s) 1069
StyI CCWWGG 3 cut(s) 754, 772, 1208
TaaI ACNGT 2 cut(s) 130, 1163
TaiI ACGT 2 cut(s) 31, 658
TaqI TCGA 2 cut(s) 55, 779
TasI AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
TatI WGTACW 2 cut(s) 250, 566
Tru1I TTAA 5 cut(s) 116, 263, 627, 693, 1086
Tru9I TTAA 5 cut(s) 116, 263, 627, 693, 1086
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 2 cut(s) 785, 905
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 6 cut(s) 281, 298, 416, 513, 733, 738
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 1 cut(s) 55
Vha464I CTTAAG 1 cut(s) 115
VpaK11BI GGWCC 2 cut(s) 1163, 1181
XapI RAATTY 2 cut(s) 72, 798
XceI RCATGY 1 cut(s) 567
XmaJI CCTAGG 1 cut(s) 1208
XspI CTAG 2 cut(s) 1031, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.