Rh1DG342400

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001926
Physical Location & Seq
Forward (+)
56718884 .. 56722458
3575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG342400.1

Sequence Viewer

Length: 1398 bp
ATGATGGCAGTGAAAAATGAATACACATGTTATGTTGATAATACTCTCAATCAGATTCTACGAGTTCTTAAACCCTCACTGGAAGATTGGGAAACACGACTGAGAATCCTTCATGAGATACAAGGAGCTGTTGAATCTGTGGAGGGTTTAAGAGGTGCGACTGTGGAGCCATTTGGATCATTCGTGTCTAATCTTTATGCTTGCTGGGGAGACTTGGATATTTCAATTGAGTTTTCCAAAGGCTCATACATTTCAGAGTATGGGAAAAAGCACAAAGAACAATTACTAGGAGTTGTGATGAGAGCTATGAGACAGAGAGGTGGATGGAAAAGTTTTAAATTTATACCCACTGCAAGAGTTCCCATATTAAAGGTCGAAAGTTATATCCAAAACATCTCTTGCGATATATCAATCAATAATCTGAAGGGACAAATGAAATCCAAACTCTTGTTTTGTATTGGTGAGATTGATGGGCGCTTCCGAGATATGGTTTTACTGGTAAAGGAATGGGCAAAAGCACAAAATATGAATAATCCAAGACTTGGGTCTTTCAACTCATACGCCCTCACTTTGCTTGTGGTCTTCCACTTTCAGACATGTACACCTGCAATTTTGCCACCTCTTAAAGATATATATCCAGGGAATCTCACGGATGATCTTCAAGGCTTGAGGGCTGATGCAGAAAAACGTATTGAAGAAACATGTGTTGATAACGTAACAAGGTTTAAGTCAGACAAATTTGGAGCAGAAAACAGAAGTTCATTGTCACAGCTTTTCATTTCATTCCTTGAGAAGTTTTCGGACCTTGTTCTGAAAGCAAGAAAGTTGGGAATTTGCCCATATACCGGACAGTGGGAAGACATACCAAGCAATATGACATGGTTGCCCCAAACATATACCATAATGATTGAAGATCCCTTTGATCAGCCAGAAAACTCTTCAAGGGCTGTTAGCATGGGAGAATTGACAAGGATATCTGAAGCTTTTAAGATGAGCAGCCACATACTCGTGTCAGCTAATCAGAGTGACATTCTTGCCACACTAGTCCGACCCCTAACCTACCGCCTTGTGACAGGAACATCTCACTACCAGTTTGAGCATCGCCATGGAGCTTATCCACAACGTTTCATGACGGAGAGCCCACATTCAAGAACACCAAGGGGGAATTATGTTCCCAGGCAACCACGTCGTCCTCAGGTGCATAAGTGGGCACAGAAACCCTCACAAGTCCAGCCTCAATTTCAGAAGATGAAGCCAGAAAACCATCCTGTCAAGCCCACTTTTCAAGAGATGAAGCCAGAAAACCATCCCAACAGGTCCACCCCTGATAACCCAACTCTAGAGACATCTCAGGATTGTGCATGCGGAATGTGTATGCCTGGGGAGTACATTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

465

Amino Acids

53.22

Weight (kDa)

8.66

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 14 - 153 1.3e-21 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 613
Acc36I ACCTGC 1 cut(s) 613
AccB7I CCANNNNNTGG 1 cut(s) 542
AciI CCGC 2 cut(s) 1063, 1365
AclI AACGTT 1 cut(s) 1123
AclWI GGATC 2 cut(s) 184, 908
AcsI RAATTY 3 cut(s) 338, 737, 831
AcuI CTGAAG 2 cut(s) 443, 999
AfaI GTAC 2 cut(s) 601, 1388
AfiI CCNNNNNNNGG 4 cut(s) 487, 542, 845, 852
AflIII ACRYGT 3 cut(s) 26, 596, 701
AgsI TTSAA 9 cut(s) 134, 225, 553, 662, 695, 911, 942, 1149, 1286
AhlI ACTAGT 1 cut(s) 1042
AjiI CACGTC 1 cut(s) 1187
AjnI CCWGG 3 cut(s) 637, 1175, 1378
AluBI AGCT 6 cut(s) 128, 305, 772, 983, 1016, 1112
AluI AGCT 6 cut(s) 128, 305, 772, 983, 1016, 1112
Alw26I GTCTC 3 cut(s) 204, 304, 1337
AlwI GGATC 2 cut(s) 184, 908
ApeKI GCWGC 1 cut(s) 996
ApoI RAATTY 3 cut(s) 338, 737, 831
AspLEI GCGC 1 cut(s) 477
AspS9I GGNCC 2 cut(s) 802, 1317
AsuHPI GGTGA 1 cut(s) 473
AvaII GGWCC 2 cut(s) 802, 1317
AxyI CCTNAGG 1 cut(s) 1194
BaeGI GKGCMC 1 cut(s) 1213
BanII GRGCYC 1 cut(s) 1142
BauI CACGAG 1 cut(s) 1007
BbsI GAAGAC 2 cut(s) 574, 864
BbvI GCAGC 1 cut(s) 1008
BccI CCATC 4 cut(s) 318, 464, 1272, 1314
BcgI CGANNNNNNTGC 2 cut(s) 1169, 1203
BciT130I CCWGG 3 cut(s) 639, 1177, 1380
BclI TGATCA 1 cut(s) 922
BcoDI GTCTC 3 cut(s) 204, 304, 1337
BcuI ACTAGT 1 cut(s) 1042
BfaI CTAG 3 cut(s) 287, 1043, 1340
BfoI RGCGCY 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 613
BisI GCNGC 1 cut(s) 997
BlsI GCNGC 1 cut(s) 998
Bme1390I CCNGG 3 cut(s) 639, 1177, 1380
Bme18I GGWCC 2 cut(s) 802, 1317
BmgBI CACGTC 1 cut(s) 1187
BmgT120I GGNCC 2 cut(s) 802, 1317
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 3 cut(s) 639, 1177, 1380
BmsI GCATC 2 cut(s) 667, 1108
BoxI GACNNNNGTC 1 cut(s) 544
BpiI GAAGAC 2 cut(s) 574, 864
BpuEI CTTGAG 2 cut(s) 688, 809
BsaBI GATNNNNATC 1 cut(s) 633
BsaJI CCNNGG 5 cut(s) 638, 1105, 1157, 1175, 1379
BsaWI WCCGGW 1 cut(s) 845
Bsc4I CCNNNNNNNGG 4 cut(s) 487, 542, 845, 852
Bse1I ACTGG 3 cut(s) 84, 501, 1090
Bse21I CCTNAGG 1 cut(s) 1194
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 3 cut(s) 639, 1177, 1380
BseDI CCNNGG 5 cut(s) 638, 1105, 1157, 1175, 1379
BseGI GGATG 4 cut(s) 329, 658, 1264, 1306
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 4 cut(s) 487, 542, 845, 852
BseMII CTCAG 3 cut(s) 92, 1208, 1364
BseNI ACTGG 3 cut(s) 84, 501, 1090
BseSI GKGCMC 1 cut(s) 1213
BseXI GCAGC 1 cut(s) 1008
BseYI CCCAGC 1 cut(s) 204
BsiSI CCGG 1 cut(s) 846
BslFI GGGAC 1 cut(s) 441
BslI CCNNNNNNNGG 4 cut(s) 487, 542, 845, 852
BsmAI GTCTC 3 cut(s) 204, 304, 1337
BsmFI GGGAC 1 cut(s) 441
Bsp1286I GDGCHC 2 cut(s) 1142, 1213
Bsp1407I TGTACA 1 cut(s) 599
Bsp143I GATC 4 cut(s) 176, 655, 913, 922
Bsp19I CCATGG 1 cut(s) 1105
BspACI CCGC 2 cut(s) 1063, 1365
BspCNI CTCAG 3 cut(s) 93, 1207, 1363
BspHI TCATGA 2 cut(s) 112, 1128
BspLI GGNNCC 1 cut(s) 168
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 2 cut(s) 184, 908
BsrGI TGTACA 1 cut(s) 599
BsrI ACTGG 3 cut(s) 84, 501, 1090
BssECI CCNNGG 5 cut(s) 638, 1105, 1157, 1175, 1379
BssMI GATC 4 cut(s) 176, 655, 913, 922
BssSI CACGAG 1 cut(s) 1007
BssT1I CCWWGG 2 cut(s) 1105, 1157
Bst2BI CACGAG 1 cut(s) 1007
Bst2UI CCWGG 3 cut(s) 639, 1177, 1380
Bst4CI ACNGT 2 cut(s) 163, 852
Bst6I CTCTTC 1 cut(s) 943
BstAUI TGTACA 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 202, 1363
BstDEI CTNAG 3 cut(s) 101, 1194, 1350
BstDSI CCRYGG 1 cut(s) 1105
BstF5I GGATG 4 cut(s) 329, 658, 1264, 1306
BstH2I RGCGCY 1 cut(s) 478
BstHHI GCGC 1 cut(s) 477
BstKTI GATC 4 cut(s) 179, 658, 916, 925
BstMAI GTCTC 3 cut(s) 204, 304, 1337
BstMBI GATC 4 cut(s) 176, 655, 913, 922
BstNI CCWGG 3 cut(s) 639, 1177, 1380
BstNSI RCATGY 4 cut(s) 30, 600, 705, 1365
BstPAI GACNNNNGTC 1 cut(s) 544
BstSCI CCNGG 3 cut(s) 637, 1175, 1378
BstSLI GKGCMC 1 cut(s) 1213
BstV1I GCAGC 1 cut(s) 1008
BstV2I GAAGAC 2 cut(s) 574, 864
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
Bsu36I CCTNAGG 1 cut(s) 1194
BtgI CCRYGG 1 cut(s) 1105
BtgZI GCGATG 1 cut(s) 1085
BtrI CACGTC 1 cut(s) 1187
BtsCI GGATG 4 cut(s) 329, 658, 1264, 1306
BtsI GCAGTG 2 cut(s) 15, 348
BtsIMutI CAGTG 4 cut(s) 15, 77, 348, 857
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 2 cut(s) 202, 1363
CciI TCATGA 2 cut(s) 112, 1128
CfoI GCGC 1 cut(s) 477
Cfr13I GGNCC 2 cut(s) 802, 1317
Csp6I GTAC 2 cut(s) 600, 1387
CviAII CATG 9 cut(s) 27, 113, 597, 702, 879, 955, 1106, 1129, 1362
CviQI GTAC 2 cut(s) 600, 1387
DdeI CTNAG 3 cut(s) 101, 1194, 1350
DpnI GATC 4 cut(s) 178, 657, 915, 924
DpnII GATC 4 cut(s) 176, 655, 913, 922
DraI TTTAAA 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 943
EarI CTCTTC 1 cut(s) 943
Eco130I CCWWGG 2 cut(s) 1105, 1157
Eco24I GRGCYC 1 cut(s) 1142
Eco32I GATATC 1 cut(s) 975
Eco47I GGWCC 2 cut(s) 802, 1317
Eco57I CTGAAG 2 cut(s) 443, 999
Eco81I CCTNAGG 1 cut(s) 1194
EcoRII CCWGG 3 cut(s) 637, 1175, 1378
EcoRV GATATC 1 cut(s) 975
EcoT14I CCWWGG 2 cut(s) 1105, 1157
EcoT38I GRGCYC 1 cut(s) 1142
ErhI CCWWGG 2 cut(s) 1105, 1157
FaeI CATG 9 cut(s) 30, 116, 600, 705, 882, 958, 1109, 1132, 1365
FaqI GGGAC 1 cut(s) 441
FatI CATG 9 cut(s) 26, 112, 596, 701, 878, 954, 1105, 1128, 1361
FbaI TGATCA 1 cut(s) 922
Fnu4HI GCNGC 1 cut(s) 997
FokI GGATG 4 cut(s) 336, 665, 1251, 1293
FriOI GRGCYC 1 cut(s) 1142
Fsp4HI GCNGC 1 cut(s) 997
FspBI CTAG 3 cut(s) 287, 1043, 1340
GlaI GCGC 1 cut(s) 476
GluI GCNGC 1 cut(s) 997
GsaI CCCAGC 1 cut(s) 208
HaeII RGCGCY 1 cut(s) 478
HapII CCGG 1 cut(s) 846
HhaI GCGC 1 cut(s) 477
Hin1II CATG 9 cut(s) 30, 116, 600, 705, 882, 958, 1109, 1132, 1365
Hin6I GCGC 1 cut(s) 475
HinP1I GCGC 1 cut(s) 475
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 4 cut(s) 55, 105, 134, 643
HpaII CCGG 1 cut(s) 846
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 2 cut(s) 602, 1320
Hpy188III TCNNGA 6 cut(s) 113, 1129, 1149, 1286, 1340, 1352
Hpy8I GTNNAC 2 cut(s) 602, 1320
Hpy99I CGWCG 1 cut(s) 1191
HpyAV CCTTC 2 cut(s) 119, 418
HpyCH4III ACNGT 2 cut(s) 163, 852
HpyCH4IV ACGT 4 cut(s) 688, 714, 1123, 1186
HpyCH4V TGCA 5 cut(s) 353, 608, 680, 1201, 1361
HpyF3I CTNAG 3 cut(s) 101, 1194, 1350
HpySE526I ACGT 4 cut(s) 688, 714, 1123, 1186
Hsp92II CATG 9 cut(s) 30, 116, 600, 705, 882, 958, 1109, 1132, 1365
HspAI GCGC 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 4 cut(s) 176, 655, 913, 922
LmnI GCTCC 4 cut(s) 125, 166, 743, 1109
Lsp1109I GCAGC 1 cut(s) 1008
LweI GCATC 2 cut(s) 667, 1108
MaeI CTAG 3 cut(s) 287, 1043, 1340
MaeII ACGT 4 cut(s) 688, 714, 1123, 1186
MaeIII GTNAC 4 cut(s) 715, 765, 1025, 1069
MalI GATC 4 cut(s) 178, 657, 915, 924
MboI GATC 4 cut(s) 176, 655, 913, 922
MboII GAAGA 8 cut(s) 95, 574, 650, 707, 869, 923, 930, 1258
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 2 cut(s) 1142, 1213
MluCI AATT 9 cut(s) 225, 281, 338, 609, 737, 831, 962, 1165, 1238
MmeI TCCRAC 1 cut(s) 1072
MseI TTAA 7 cut(s) 69, 149, 336, 368, 624, 726, 987
MslI CAYNNNNRTG 2 cut(s) 1007, 1104
MspI CCGG 1 cut(s) 846
MspR9I CCNGG 3 cut(s) 639, 1177, 1380
MunI CAATTG 1 cut(s) 225
MvaI CCWGG 3 cut(s) 639, 1177, 1380
NcoI CCATGG 1 cut(s) 1105
NdeII GATC 4 cut(s) 176, 655, 913, 922
NlaIII CATG 9 cut(s) 30, 116, 600, 705, 882, 958, 1109, 1132, 1365
NlaIV GGNNCC 1 cut(s) 168
NmuCI GTSAC 3 cut(s) 765, 1025, 1069
NspI RCATGY 4 cut(s) 30, 600, 705, 1365
PaeI GCATGC 1 cut(s) 1365
PagI TCATGA 2 cut(s) 112, 1128
PaqCI CACCTGC 1 cut(s) 613
PciI ACATGT 3 cut(s) 26, 596, 701
PfeI GAWTC 4 cut(s) 55, 105, 134, 643
PflMI CCANNNNNTGG 1 cut(s) 542
PkrI GCNGC 1 cut(s) 998
PscI ACATGT 3 cut(s) 26, 596, 701
PshAI GACNNNNGTC 1 cut(s) 544
Psp1406I AACGTT 1 cut(s) 1123
Psp6I CCWGG 3 cut(s) 637, 1175, 1378
PspFI CCCAGC 1 cut(s) 204
PspGI CCWGG 3 cut(s) 637, 1175, 1378
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 2 cut(s) 802, 1317
PsuI RGATCY 1 cut(s) 913
RsaI GTAC 2 cut(s) 601, 1388
RsaNI GTAC 2 cut(s) 600, 1387
RseI CAYNNNNRTG 2 cut(s) 1007, 1104
SaqAI TTAA 7 cut(s) 69, 149, 336, 368, 624, 726, 987
SatI GCNGC 1 cut(s) 997
Sau3AI GATC 4 cut(s) 176, 655, 913, 922
Sau96I GGNCC 2 cut(s) 802, 1317
ScrFI CCNGG 3 cut(s) 639, 1177, 1380
SduI GDGCHC 2 cut(s) 1142, 1213
SfaNI GCATC 2 cut(s) 667, 1108
SinI GGWCC 2 cut(s) 802, 1317
SmiMI CAYNNNNRTG 2 cut(s) 1007, 1104
SmlI CTYRAG 2 cut(s) 667, 788
SmoI CTYRAG 2 cut(s) 667, 788
SpeI ACTAGT 1 cut(s) 1042
SphI GCATGC 1 cut(s) 1365
Sse9I AATT 9 cut(s) 225, 281, 338, 609, 737, 831, 962, 1165, 1238
SsiI CCGC 2 cut(s) 1063, 1365
SspMI CTAG 3 cut(s) 287, 1043, 1340
StyD4I CCNGG 3 cut(s) 637, 1175, 1378
StyI CCWWGG 2 cut(s) 1105, 1157
TaaI ACNGT 2 cut(s) 163, 852
TaiI ACGT 4 cut(s) 691, 717, 1126, 1189
TaqI TCGA 1 cut(s) 375
TasI AATT 9 cut(s) 225, 281, 338, 609, 737, 831, 962, 1165, 1238
TatI WGTACW 2 cut(s) 599, 1386
TfiI GAWTC 4 cut(s) 55, 105, 134, 643
Tru1I TTAA 7 cut(s) 69, 149, 336, 368, 624, 726, 987
Tru9I TTAA 7 cut(s) 69, 149, 336, 368, 624, 726, 987
TscAI CASTG 4 cut(s) 15, 84, 355, 857
TseFI GTSAC 3 cut(s) 765, 1025, 1069
TseI GCWGC 1 cut(s) 996
Tsp45I GTSAC 3 cut(s) 765, 1025, 1069
TspGWI ACGGA 2 cut(s) 665, 1148
TspRI CASTG 4 cut(s) 15, 84, 355, 857
Van91I CCANNNNNTGG 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 802, 1317
XapI RAATTY 3 cut(s) 338, 737, 831
XbaI TCTAGA 1 cut(s) 1339
XceI RCATGY 4 cut(s) 30, 600, 705, 1365
XspI CTAG 3 cut(s) 287, 1043, 1340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.