Rroxscaffold_4G00288660

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001926
Physical Location & Seq
Reverse (-)
9349323 .. 9354421
5099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00288660.1

Sequence Viewer

Length: 1383 bp
ATGAATACACATGTTATGTTGGATAATACTCTCAATCAGATTCTACGAGTTCTTAAACCCTCACTGGAAGATTGGGAAACACGACTGAGAATCCTTCATGATTTACAAGGAGCTGTTGAATCTGTGGAGGGTTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTCGTGTCTAATCTGTATGCTTGCTGGGGAGACTTGGATATTTCAATTGAGTTTTCCAAAGGCTCATATATTTCAGAGTATGGGAAAAAGCACAAAGAACAATTACTAGGAGTTGTGATGAGAGCTCTGAGACAGAGAGGTGGATGGAAAAGTTTTAAATTTATACCCACTGCAAGAGTTCCCATATTAAAGGTCGAAAGTTATCTCCAAAACATCTCTTGCGATATATCAATCAATAATCTGAAGGGACAAATGAAATCCAAACTCTTGTTTTGTATTGGTGAGATTGATGGGCGCTTCCGAGTTATGGTTTTACTGGTAAAGGAATGGGCAAAAGCACAAAATATGAATAATCCAAGACTCGGGTCTTTCAACTCATACGCCCTCACTTTGCTTGTGGTCTTCCACTTTCAGACATGTACACCAGCAATTTTGCCACCTCTTAAAGATATATATCCAGGGAATCTCACGGATGATCTTCAAGGCTTGAGGGCTGATGCAGAAAAACGTATTGAAGAAACATGTGTTGATAACGTAACAAGGTTTAAATCAGACAAATTTGGAGCAGAAAACAGAAGTTCATTGTCACAGCTTTTCATTTCATTCCTTGAGAAGTTTTCGGACCTTGTTTTGAAAACAAGAAAGTTGGGAATTTGCCCATATACTGGACAGTGGGAAGACATACCAAGCAATATGACATGGTTGCCCCAAACATATACCATAATGATTGAAGATCCCTTTGATCAGCCAGAAAACTCTTCAAGGGCTGTTAGCATGGGAGAATTGACAAGGATATCTGAAGCTTTTAAGATGAGCAGCCACATACTCATGTCAGCTAATCAGAGTGACATTCTTGCCACACTAGTCCGACCCCTAACCTACCGTCTTGTGACAGGAACATCTCACTACCAGTTTGAGCATCGCCATGCAGCTTATCCACAACGGTTCATGAATGAGAGCCCACATTCAAGAACACCAAGGGGGAATTATGTTCCCAGGCAACCTCGTCGTCCTCAGGTGCATAAGTGGGCACAGAAACCCTCACAAGTCCAGCCTCAATTTCAGAAGATGAAGCCAGAAAACCATCCTGTCAAGCCCACTTTTCAAGAGATGAAGCCAGAAAACCATCCCGACAGGTCCACCCCTGATAACCCAACTCTAGAGACATCTCAGGATTGTGCATGCGGAATGTGTATGCCTGGGGAGTACATTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

460

Amino Acids

52.65

Weight (kDa)

8.71

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 7 - 148 4e-22 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 830
AciI CCGC 1 cut(s) 1350
AclWI GGATC 2 cut(s) 169, 893
AcsI RAATTY 3 cut(s) 323, 722, 816
AcuI CTGAAG 2 cut(s) 428, 984
AfaI GTAC 2 cut(s) 586, 1373
AfiI CCNNNNNNNGG 3 cut(s) 472, 527, 830
AflIII ACRYGT 3 cut(s) 10, 581, 686
AhlI ACTAGT 1 cut(s) 1027
AjnI CCWGG 3 cut(s) 622, 1160, 1363
AluBI AGCT 6 cut(s) 113, 290, 757, 968, 1001, 1097
AluI AGCT 6 cut(s) 113, 290, 757, 968, 1001, 1097
Alw21I GWGCWC 1 cut(s) 292
Alw26I GTCTC 3 cut(s) 189, 289, 1322
AlwI GGATC 2 cut(s) 169, 893
Ama87I CYCGRG 1 cut(s) 527
ApeKI GCWGC 2 cut(s) 981, 1094
ApoI RAATTY 3 cut(s) 323, 722, 816
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 2 cut(s) 787, 1302
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 527
AvaII GGWCC 2 cut(s) 787, 1302
AxyI CCTNAGG 1 cut(s) 1179
BaeGI GKGCMC 1 cut(s) 1198
BanII GRGCYC 2 cut(s) 292, 1127
BbsI GAAGAC 2 cut(s) 559, 849
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 2 cut(s) 993, 1106
BccI CCATC 4 cut(s) 303, 449, 1257, 1299
BcgI CGANNNNNNTGC 2 cut(s) 1154, 1188
BciT130I CCWGG 3 cut(s) 624, 1162, 1365
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 3 cut(s) 189, 289, 1322
BcuI ACTAGT 1 cut(s) 1027
BfaI CTAG 3 cut(s) 272, 1028, 1325
BfoI RGCGCY 1 cut(s) 463
BisI GCNGC 2 cut(s) 982, 1095
BlsI GCNGC 2 cut(s) 983, 1096
Bme1390I CCNGG 3 cut(s) 624, 1162, 1365
Bme18I GGWCC 2 cut(s) 787, 1302
BmeT110I CYCGRG 1 cut(s) 527
BmgT120I GGNCC 2 cut(s) 787, 1302
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 3 cut(s) 624, 1162, 1365
BmsI GCATC 2 cut(s) 652, 1093
BoxI GACNNNNGTC 1 cut(s) 529
BpiI GAAGAC 2 cut(s) 559, 849
BpuEI CTTGAG 2 cut(s) 673, 794
BsaBI GATNNNNATC 1 cut(s) 618
BsaJI CCNNGG 4 cut(s) 623, 1142, 1160, 1364
Bsc4I CCNNNNNNNGG 3 cut(s) 472, 527, 830
Bse1I ACTGG 4 cut(s) 69, 486, 835, 1075
Bse21I CCTNAGG 1 cut(s) 1179
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 3 cut(s) 624, 1162, 1365
BseDI CCNNGG 4 cut(s) 623, 1142, 1160, 1364
BseGI GGATG 4 cut(s) 314, 643, 1249, 1291
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 3 cut(s) 472, 527, 830
BseMII CTCAG 4 cut(s) 77, 284, 1193, 1349
BseNI ACTGG 4 cut(s) 69, 486, 835, 1075
BseSI GKGCMC 1 cut(s) 1198
BseXI GCAGC 2 cut(s) 993, 1106
BseYI CCCAGC 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 292
BsiHKCI CYCGRG 1 cut(s) 527
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 3 cut(s) 472, 527, 830
BsmAI GTCTC 3 cut(s) 189, 289, 1322
BsmFI GGGAC 1 cut(s) 426
BsoBI CYCGRG 1 cut(s) 527
Bsp1286I GDGCHC 3 cut(s) 292, 1127, 1198
Bsp1407I TGTACA 1 cut(s) 584
Bsp143I GATC 4 cut(s) 161, 640, 898, 907
BspACI CCGC 1 cut(s) 1350
BspCNI CTCAG 4 cut(s) 78, 285, 1192, 1348
BspHI TCATGA 2 cut(s) 97, 1113
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 2 cut(s) 169, 893
BsrGI TGTACA 1 cut(s) 584
BsrI ACTGG 4 cut(s) 69, 486, 835, 1075
BssECI CCNNGG 4 cut(s) 623, 1142, 1160, 1364
BssMI GATC 4 cut(s) 161, 640, 898, 907
BssT1I CCWWGG 1 cut(s) 1142
Bst2UI CCWGG 3 cut(s) 624, 1162, 1365
Bst4CI ACNGT 4 cut(s) 148, 837, 1049, 1110
Bst6I CTCTTC 1 cut(s) 928
BstAUI TGTACA 1 cut(s) 584
BstC8I GCNNGC 2 cut(s) 187, 1348
BstDEI CTNAG 4 cut(s) 86, 293, 1179, 1335
BstF5I GGATG 4 cut(s) 314, 643, 1249, 1291
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 4 cut(s) 164, 643, 901, 910
BstMAI GTCTC 3 cut(s) 189, 289, 1322
BstMBI GATC 4 cut(s) 161, 640, 898, 907
BstNI CCWGG 3 cut(s) 624, 1162, 1365
BstNSI RCATGY 4 cut(s) 14, 585, 690, 1350
BstPAI GACNNNNGTC 1 cut(s) 529
BstSCI CCNGG 3 cut(s) 622, 1160, 1363
BstSLI GKGCMC 1 cut(s) 1198
BstV1I GCAGC 2 cut(s) 993, 1106
BstV2I GAAGAC 2 cut(s) 559, 849
BstX2I RGATCY 1 cut(s) 898
BstYI RGATCY 1 cut(s) 898
Bsu36I CCTNAGG 1 cut(s) 1179
BtgZI GCGATG 1 cut(s) 1070
BtsCI GGATG 4 cut(s) 314, 643, 1249, 1291
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 3 cut(s) 62, 333, 842
Cac8I GCNNGC 2 cut(s) 187, 1348
CciI TCATGA 2 cut(s) 97, 1113
CfoI GCGC 1 cut(s) 462
Cfr13I GGNCC 2 cut(s) 787, 1302
Csp6I GTAC 2 cut(s) 585, 1372
CviQI GTAC 2 cut(s) 585, 1372
DdeI CTNAG 4 cut(s) 86, 293, 1179, 1335
DpnI GATC 4 cut(s) 163, 642, 900, 909
DpnII GATC 4 cut(s) 161, 640, 898, 907
DraI TTTAAA 2 cut(s) 322, 712
Eam1104I CTCTTC 1 cut(s) 928
EarI CTCTTC 1 cut(s) 928
Ecl136II GAGCTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 1142
Eco24I GRGCYC 2 cut(s) 292, 1127
Eco32I GATATC 1 cut(s) 960
Eco47I GGWCC 2 cut(s) 787, 1302
Eco53kI GAGCTC 1 cut(s) 290
Eco57I CTGAAG 2 cut(s) 428, 984
Eco81I CCTNAGG 1 cut(s) 1179
Eco88I CYCGRG 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 290
EcoRII CCWGG 3 cut(s) 622, 1160, 1363
EcoRV GATATC 1 cut(s) 960
EcoT14I CCWWGG 1 cut(s) 1142
EcoT38I GRGCYC 2 cut(s) 292, 1127
ErhI CCWWGG 1 cut(s) 1142
FaqI GGGAC 1 cut(s) 426
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 2 cut(s) 982, 1095
FokI GGATG 4 cut(s) 321, 650, 1236, 1278
FriOI GRGCYC 2 cut(s) 292, 1127
Fsp4HI GCNGC 2 cut(s) 982, 1095
FspBI CTAG 3 cut(s) 272, 1028, 1325
GlaI GCGC 1 cut(s) 461
GluI GCNGC 2 cut(s) 982, 1095
GsaI CCCAGC 1 cut(s) 193
HaeII RGCGCY 1 cut(s) 463
HhaI GCGC 1 cut(s) 462
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 966
HinfI GANTC 5 cut(s) 40, 90, 119, 525, 628
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 2 cut(s) 587, 1305
Hpy188III TCNNGA 7 cut(s) 98, 1114, 1134, 1271, 1295, 1325, 1337
Hpy8I GTNNAC 2 cut(s) 587, 1305
Hpy99I CGWCG 1 cut(s) 1176
HpyAV CCTTC 2 cut(s) 104, 403
HpyCH4III ACNGT 4 cut(s) 148, 837, 1049, 1110
HpyCH4IV ACGT 2 cut(s) 673, 699
HpyCH4V TGCA 6 cut(s) 143, 338, 665, 1094, 1186, 1346
HpyF3I CTNAG 4 cut(s) 86, 293, 1179, 1335
HpySE526I ACGT 2 cut(s) 673, 699
HspAI GCGC 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 4 cut(s) 161, 640, 898, 907
LmnI GCTCC 3 cut(s) 110, 151, 728
Lsp1109I GCAGC 2 cut(s) 993, 1106
LweI GCATC 2 cut(s) 652, 1093
MaeI CTAG 3 cut(s) 272, 1028, 1325
MaeII ACGT 2 cut(s) 673, 699
MaeIII GTNAC 4 cut(s) 700, 750, 1010, 1054
MalI GATC 4 cut(s) 163, 642, 900, 909
MboI GATC 4 cut(s) 161, 640, 898, 907
MboII GAAGA 8 cut(s) 80, 559, 635, 692, 854, 908, 915, 1243
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 898
MhlI GDGCHC 3 cut(s) 292, 1127, 1198
MluCI AATT 9 cut(s) 210, 266, 323, 594, 722, 816, 947, 1150, 1223
MlyI GAGTC 1 cut(s) 519
MmeI TCCRAC 1 cut(s) 1057
MseI TTAA 7 cut(s) 54, 134, 321, 353, 609, 711, 972
MslI CAYNNNNRTG 2 cut(s) 992, 1089
MspR9I CCNGG 3 cut(s) 624, 1162, 1365
MunI CAATTG 1 cut(s) 210
MvaI CCWGG 3 cut(s) 624, 1162, 1365
NdeII GATC 4 cut(s) 161, 640, 898, 907
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 3 cut(s) 750, 1010, 1054
NspI RCATGY 4 cut(s) 14, 585, 690, 1350
PaeI GCATGC 1 cut(s) 1350
PagI TCATGA 2 cut(s) 97, 1113
PciI ACATGT 3 cut(s) 10, 581, 686
PfeI GAWTC 4 cut(s) 40, 90, 119, 628
PflMI CCANNNNNTGG 1 cut(s) 830
PkrI GCNGC 2 cut(s) 983, 1096
PleI GAGTC 1 cut(s) 519
PpsI GAGTC 1 cut(s) 519
PscI ACATGT 3 cut(s) 10, 581, 686
PshAI GACNNNNGTC 1 cut(s) 529
Psp124BI GAGCTC 1 cut(s) 292
Psp6I CCWGG 3 cut(s) 622, 1160, 1363
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 3 cut(s) 622, 1160, 1363
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 787, 1302
PsuI RGATCY 1 cut(s) 898
RsaI GTAC 2 cut(s) 586, 1373
RsaNI GTAC 2 cut(s) 585, 1372
RseI CAYNNNNRTG 2 cut(s) 992, 1089
SacI GAGCTC 1 cut(s) 292
SaqAI TTAA 7 cut(s) 54, 134, 321, 353, 609, 711, 972
SatI GCNGC 2 cut(s) 982, 1095
Sau3AI GATC 4 cut(s) 161, 640, 898, 907
Sau96I GGNCC 2 cut(s) 787, 1302
SchI GAGTC 1 cut(s) 519
ScrFI CCNGG 3 cut(s) 624, 1162, 1365
SduI GDGCHC 3 cut(s) 292, 1127, 1198
SfaNI GCATC 2 cut(s) 652, 1093
SinI GGWCC 2 cut(s) 787, 1302
SmiMI CAYNNNNRTG 2 cut(s) 992, 1089
SmlI CTYRAG 2 cut(s) 652, 773
SmoI CTYRAG 2 cut(s) 652, 773
SpeI ACTAGT 1 cut(s) 1027
SphI GCATGC 1 cut(s) 1350
Sse9I AATT 9 cut(s) 210, 266, 323, 594, 722, 816, 947, 1150, 1223
SsiI CCGC 1 cut(s) 1350
SspMI CTAG 3 cut(s) 272, 1028, 1325
SstI GAGCTC 1 cut(s) 292
StyD4I CCNGG 3 cut(s) 622, 1160, 1363
StyI CCWWGG 1 cut(s) 1142
TaaI ACNGT 4 cut(s) 148, 837, 1049, 1110
TaiI ACGT 2 cut(s) 676, 702
TaqI TCGA 1 cut(s) 360
TasI AATT 9 cut(s) 210, 266, 323, 594, 722, 816, 947, 1150, 1223
TatI WGTACW 2 cut(s) 584, 1371
TfiI GAWTC 4 cut(s) 40, 90, 119, 628
Tru1I TTAA 7 cut(s) 54, 134, 321, 353, 609, 711, 972
Tru9I TTAA 7 cut(s) 54, 134, 321, 353, 609, 711, 972
TscAI CASTG 3 cut(s) 69, 340, 842
TseFI GTSAC 3 cut(s) 750, 1010, 1054
TseI GCWGC 2 cut(s) 981, 1094
Tsp45I GTSAC 3 cut(s) 750, 1010, 1054
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 3 cut(s) 69, 340, 842
Van91I CCANNNNNTGG 1 cut(s) 830
VpaK11BI GGWCC 2 cut(s) 787, 1302
XapI RAATTY 3 cut(s) 323, 722, 816
XbaI TCTAGA 1 cut(s) 1324
XceI RCATGY 4 cut(s) 14, 585, 690, 1350
XspI CTAG 3 cut(s) 272, 1028, 1325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.