RLG00000027220

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001926
Physical Location & Seq
Reverse (-)
9681801 .. 9685397
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027220

Sequence Viewer

Length: 1554 bp
ATGGAGAATACTCTCAGGGAGATAATACGTGTGCTTGAACCCTTGCCCACGGATCGAGAAACACGATGCCAAATTCTTCGGGAGTTACAAGGAGCTGTTGAGTCTGTAGAAAGCTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTTGTCTCAGAACTTTTTACTCGCTGGGGAGACTTGGATGTTTCAATTGAGTTCTCTAAAGGCGCATATATTTCTTCTTATGGAAGAAAGCACACACAAGATGTACTAAAAGATTTAATGAAGGCTATGAGAAAGAGAGGTGGATGGAACAAGTATCAGTTCATACCTAATGCAAGGGTTCCCATTCTGATGGTCAAAAGTAATCTTGGAAACATCTCCTGTGATATTTCAGTCAATAACCTGAAAGCCCAAATGAAATCAAAACTCTTTTTATGGATCAATGATATAGATCCACGCTTTCGTGATATGGTTTTACTGGTGAAGGAATGGGCAAAAGCACACAATATCAATAATTCAAAATCTGGGACTTTCAATTCATACTCTCTGAGTTTGCTTGTGATCTTCCATTTTCAGACATGTACACCTGCAATTCTGCCTCCTCTTCAAGACATATATACAGAAACTCTCACGGATGATCTTAAAGGCTTGAGGGATGATGCAGAAAGTCGTATTGAAAAAAGTTGTGTTGAGAACCTGAGAAGATTTAAACTAAACAAATTGAGAGCTTGGAATAAAAGTTCTTTGTTTGAGCTTTTCATTTCATTCCTTGGGGAGTTCTCTGACCTTGGTTCGAAGGCTGCGGAGTTGGGAATTTGCACATATTCTGGAAAATGGGAAAGAATAGAAAGCAATATGGGATGGTTGCCCAAAACCTATTGTATATTGGTTGAAGATCCTTTTGATCGGCCAGAAAATACTGCAAGAGGTGTCAGCCAAAAAGCATTGAAAAGGATCTCTGAGGTTTTTGAGATGAGTCGTGACATGCTAATCGCAGCTCGTTACAATGCCTCCTTAGCCATTGAGAGTTCCCTTCTTGCCACACTAGTCCGACCACATACGCTACATCTTATTAGCAACCCAGGTTACAATATTAAGCACCACCAAACAACTCATCCACAATATCTCAGGGCTGAGAGCTCACATTCACGAACCCCAAGACATTATAACGGTCCCCGGAAACCAAATGGTCCTTCTGTACGAGAGTTGGTGCCCCCAAGGGAATATAACGGCCCCCCAAAACCAAATGGTAAGGTACGAGAGTTGGTGCCCCAAAGGGATTATAATGGCCCCCCAAAACCAAGTGGTCCTTATGGACGGGAGTTGGTAGACTCATCCCCACAAGTACATCAACAACTACAAAAGATGGTGCACTCACCGTCACAAGTACAGCCACAAATACAGAAGCTGGTGCACTCACCGTCACAAGTACAACAGCATATACGAAAGTCTGTGCCCTCAACCTCACGAGTACAATCACCACTTCAGAAAGTGAAGCCAGAGAAGGCACCAATGCTACAGGCGTATCAGGAATGGAGACCAAAGAAACCAGGAATGCAGACCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

518

Amino Acids

59.03

Weight (kDa)

9.62

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 3 - 139 2.1e-21 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1152, 1269
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 3 cut(s) 1195, 1252, 1492
AccI GTMKAC 1 cut(s) 1314
AciI CCGC 1 cut(s) 788
AclWI GGATC 6 cut(s) 60, 151, 431, 431, 875, 947
AcoI YGGCCR 1 cut(s) 893
AcsI RAATTY 2 cut(s) 72, 798
AcuI CTGAAG 1 cut(s) 1454
AfaI GTAC 8 cut(s) 252, 568, 1185, 1242, 1332, 1374, 1416, 1458
AflII CTTAAG 1 cut(s) 115
AflIII ACRYGT 2 cut(s) 28, 563
AgsI TTSAA 8 cut(s) 38, 192, 504, 520, 593, 662, 878, 934
AhlI ACTAGT 1 cut(s) 1030
AjnI CCWGG 2 cut(s) 1066, 1534
AjuI GAANNNNNNNTTGG 2 cut(s) 1162, 1194
AloI GAACNNNNNNTCC 2 cut(s) 709, 741
AluBI AGCT 7 cut(s) 95, 114, 713, 739, 983, 1125, 1393
AluI AGCT 7 cut(s) 95, 114, 713, 739, 983, 1125, 1393
Alw21I GWGCWC 3 cut(s) 1127, 1359, 1401
Alw26I GTCTC 3 cut(s) 157, 171, 1516
Alw44I GTGCAC 2 cut(s) 1355, 1397
AlwI GGATC 6 cut(s) 60, 151, 431, 431, 875, 947
AlwNI CAGNNNCTG 1 cut(s) 1393
AoxI GGCC 3 cut(s) 893, 1216, 1273
ApaLI GTGCAC 2 cut(s) 1355, 1397
ApeKI GCWGC 2 cut(s) 785, 980
ApoI RAATTY 2 cut(s) 72, 798
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 5 cut(s) 1157, 1175, 1217, 1274, 1292
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 4 cut(s) 478, 1353, 1395, 1455
AsuII TTCGAA 1 cut(s) 779
AvaII GGWCC 3 cut(s) 1157, 1175, 1292
BaeGI GKGCMC 5 cut(s) 1200, 1257, 1359, 1401, 1443
BaeI ACNNNNGTAYC 4 cut(s) 1232, 1265, 1493, 1526
BanI GGYRCC 3 cut(s) 1195, 1252, 1492
BanII GRGCYC 1 cut(s) 1127
BauI CACGAG 1 cut(s) 1452
Bbv12I GWGCWC 3 cut(s) 1127, 1359, 1401
BbvI GCAGC 2 cut(s) 772, 992
BccI CCATC 4 cut(s) 285, 331, 840, 1345
BceAI ACGGC 1 cut(s) 1231
BciT130I CCWGG 2 cut(s) 1068, 1536
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 3 cut(s) 157, 171, 1516
BcuI ACTAGT 1 cut(s) 1030
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 2 cut(s) 105, 1502
BfrI CTTAAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 2 cut(s) 786, 981
BlsI GCNGC 2 cut(s) 787, 982
Bme1390I CCNGG 3 cut(s) 1068, 1162, 1536
Bme18I GGWCC 3 cut(s) 1157, 1175, 1292
BmgT120I GGNCC 5 cut(s) 1157, 1175, 1217, 1274, 1292
BmiI GGNNCC 8 cut(s) 135, 327, 1159, 1197, 1219, 1254, 1276, 1494
BmrFI CCNGG 3 cut(s) 1068, 1162, 1536
BmsI GCATC 2 cut(s) 56, 634
Bpu10I CCTNAGC 1 cut(s) 1000
Bpu14I TTCGAA 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 655
BpuMI CCSGG 1 cut(s) 1162
BsaAI YACGTR 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 1516
BsaJI CCNNGG 6 cut(s) 48, 754, 772, 1066, 1160, 1202
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bse1I ACTGG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 2 cut(s) 1068, 1536
BseDI CCNNGG 6 cut(s) 48, 754, 772, 1066, 1160, 1202
BseGI GGATG 7 cut(s) 190, 296, 625, 646, 851, 1099, 1319
BseJI GATNNNNATC 1 cut(s) 435
BseMII CTCAG 7 cut(s) 28, 168, 524, 674, 936, 1110, 1126
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 5 cut(s) 1200, 1257, 1359, 1401, 1443
BseXI GCAGC 2 cut(s) 772, 992
BseYI CCCAGC 1 cut(s) 171
BshFI GGCC 3 cut(s) 895, 1218, 1275
BshNI GGYRCC 3 cut(s) 1195, 1252, 1492
BsiHKAI GWGCWC 3 cut(s) 1127, 1359, 1401
BsiSI CCGG 1 cut(s) 1162
BslFI GGGAC 2 cut(s) 526, 1143
BsmAI GTCTC 3 cut(s) 157, 171, 1516
BsmFI GGGAC 2 cut(s) 526, 1143
BsmI GAATGC 1 cut(s) 1545
BsnI GGCC 3 cut(s) 895, 1218, 1275
Bso31I GGTCTC 1 cut(s) 1516
Bsp119I TTCGAA 1 cut(s) 779
Bsp1286I GDGCHC 6 cut(s) 1127, 1200, 1257, 1359, 1401, 1443
Bsp1407I TGTACA 1 cut(s) 566
Bsp143I GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
BspACI CCGC 1 cut(s) 788
BspANI GGCC 3 cut(s) 895, 1218, 1275
BspCNI CTCAG 7 cut(s) 27, 167, 525, 675, 937, 1111, 1125
BspLI GGNNCC 8 cut(s) 135, 327, 1159, 1197, 1219, 1254, 1276, 1494
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 6 cut(s) 60, 151, 431, 431, 875, 947
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 3 cut(s) 1195, 1252, 1492
BspTI CTTAAG 1 cut(s) 115
BspTNI GGTCTC 1 cut(s) 1516
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 6 cut(s) 48, 754, 772, 1066, 1160, 1202
BssMI GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
BssSI CACGAG 1 cut(s) 1452
BssT1I CCWWGG 3 cut(s) 754, 772, 1202
Bst2BI CACGAG 1 cut(s) 1452
Bst2UI CCWGG 2 cut(s) 1068, 1536
Bst4CI ACNGT 4 cut(s) 130, 1157, 1365, 1407
Bst6I CTCTTC 1 cut(s) 594
BstAFI CTTAAG 1 cut(s) 115
BstAUI TGTACA 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 779
BstDEI CTNAG 8 cut(s) 14, 154, 533, 683, 945, 1000, 1112, 1119
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 7 cut(s) 190, 296, 625, 646, 851, 1099, 1319
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 9 cut(s) 55, 146, 426, 439, 549, 625, 883, 892, 942
BstMAI GTCTC 3 cut(s) 157, 171, 1516
BstMBI GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 2 cut(s) 1068, 1536
BstNSI RCATGY 2 cut(s) 567, 973
BstSCI CCNGG 3 cut(s) 1066, 1160, 1534
BstSFI CTRYAG 2 cut(s) 105, 1502
BstSLI GKGCMC 5 cut(s) 1200, 1257, 1359, 1401, 1443
BstV1I GCAGC 2 cut(s) 772, 992
BstX2I RGATCY 3 cut(s) 436, 880, 939
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 436, 880, 939
BsuRI GGCC 3 cut(s) 895, 1218, 1275
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 7 cut(s) 190, 296, 625, 646, 851, 1099, 1319
BveI ACCTGC 1 cut(s) 580
CaiI CAGNNNCTG 1 cut(s) 1393
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 5 cut(s) 1157, 1175, 1217, 1274, 1292
Csp6I GTAC 8 cut(s) 251, 567, 1184, 1241, 1331, 1373, 1415, 1457
CviAII CATG 2 cut(s) 564, 970
CviQI GTAC 8 cut(s) 251, 567, 1184, 1241, 1331, 1373, 1415, 1457
DdeI CTNAG 8 cut(s) 14, 154, 533, 683, 945, 1000, 1112, 1119
DpnI GATC 9 cut(s) 54, 145, 425, 438, 548, 624, 882, 891, 941
DpnII GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 893
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 1 cut(s) 1125
Eco130I CCWWGG 3 cut(s) 754, 772, 1202
Eco24I GRGCYC 1 cut(s) 1127
Eco31I GGTCTC 1 cut(s) 1516
Eco47I GGWCC 3 cut(s) 1157, 1175, 1292
Eco53kI GAGCTC 1 cut(s) 1125
Eco57I CTGAAG 1 cut(s) 1454
EcoICRI GAGCTC 1 cut(s) 1125
EcoRII CCWGG 2 cut(s) 1066, 1534
EcoT14I CCWWGG 3 cut(s) 754, 772, 1202
EcoT38I GRGCYC 1 cut(s) 1127
ErhI CCWWGG 3 cut(s) 754, 772, 1202
FaeI CATG 2 cut(s) 567, 973
FalI AAGNNNNNCTT 2 cut(s) 1279, 1311
FaqI GGGAC 2 cut(s) 526, 1143
FatI CATG 2 cut(s) 563, 969
FblI GTMKAC 1 cut(s) 1314
Fnu4HI GCNGC 2 cut(s) 786, 981
FokI GGATG 7 cut(s) 197, 303, 632, 653, 858, 1086, 1306
FriOI GRGCYC 1 cut(s) 1127
Fsp4HI GCNGC 2 cut(s) 786, 981
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 1 cut(s) 211
GluI GCNGC 2 cut(s) 786, 981
GsaI CCCAGC 1 cut(s) 175
HaeIII GGCC 3 cut(s) 895, 1218, 1275
HapII CCGG 1 cut(s) 1162
HhaI GCGC 1 cut(s) 212
Hin1II CATG 2 cut(s) 567, 973
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 3 cut(s) 101, 961, 1316
HpaII CCGG 1 cut(s) 1162
HphI GGTGA 4 cut(s) 478, 1353, 1395, 1455
Hpy166II GTNNAC 4 cut(s) 569, 1315, 1357, 1399
Hpy188I TCNGA 9 cut(s) 157, 336, 534, 561, 769, 946, 1037, 1473, 1553
Hpy188III TCNNGA 9 cut(s) 56, 80, 449, 593, 813, 965, 1134, 1452, 1514
Hpy8I GTNNAC 4 cut(s) 569, 1315, 1357, 1399
HpyAV CCTTC 6 cut(s) 262, 463, 775, 1028, 1188, 1483
HpyCH4III ACNGT 4 cut(s) 130, 1157, 1365, 1407
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 9 cut(s) 125, 320, 575, 647, 804, 908, 1357, 1399, 1543
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 8 cut(s) 14, 154, 533, 683, 945, 1000, 1112, 1119
HpySE526I ACGT 1 cut(s) 28
Hsp92II CATG 2 cut(s) 567, 973
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
LmnI GCTCC 2 cut(s) 92, 133
Lsp1109I GCAGC 2 cut(s) 772, 992
LweI GCATC 2 cut(s) 56, 634
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 6 cut(s) 84, 965, 986, 1070, 1365, 1407
MalI GATC 9 cut(s) 54, 145, 425, 438, 548, 624, 882, 891, 941
MboI GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
MboII GAAGA 7 cut(s) 68, 213, 243, 541, 581, 699, 890
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 3 cut(s) 436, 880, 939
MhlI GDGCHC 6 cut(s) 1127, 1200, 1257, 1359, 1401, 1443
MluCI AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
MlyI GAGTC 3 cut(s) 110, 970, 1310
MmeI TCCRAC 1 cut(s) 1060
MseI TTAA 5 cut(s) 116, 263, 627, 693, 1080
MslI CAYNNNNRTG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 115
MspI CCGG 1 cut(s) 1162
MspR9I CCNGG 3 cut(s) 1068, 1162, 1536
MunI CAATTG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 1545
MvaI CCWGG 2 cut(s) 1068, 1536
MwoI GCNNNNNNNGC 1 cut(s) 1001
NciI CCSGG 1 cut(s) 1162
NdeII GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
NlaIII CATG 2 cut(s) 567, 973
NlaIV GGNNCC 8 cut(s) 135, 327, 1159, 1197, 1219, 1254, 1276, 1494
NmuCI GTSAC 3 cut(s) 965, 1365, 1407
NspI RCATGY 2 cut(s) 567, 973
NspV TTCGAA 1 cut(s) 779
PaqCI CACCTGC 1 cut(s) 580
PciI ACATGT 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PctI GAATGC 1 cut(s) 1545
PkrI GCNGC 2 cut(s) 787, 982
PleI GAGTC 3 cut(s) 109, 969, 1310
PpsI GAGTC 3 cut(s) 109, 969, 1310
Ppu21I YACGTR 1 cut(s) 29
PscI ACATGT 1 cut(s) 563
PsiI TTATAA 2 cut(s) 1152, 1269
Psp124BI GAGCTC 1 cut(s) 1127
Psp6I CCWGG 2 cut(s) 1066, 1534
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 2 cut(s) 1066, 1534
PspN4I GGNNCC 8 cut(s) 135, 327, 1159, 1197, 1219, 1254, 1276, 1494
PspPI GGNCC 5 cut(s) 1157, 1175, 1217, 1274, 1292
PstNI CAGNNNCTG 1 cut(s) 1393
PsuI RGATCY 3 cut(s) 436, 880, 939
RsaI GTAC 8 cut(s) 252, 568, 1185, 1242, 1332, 1374, 1416, 1458
RsaNI GTAC 8 cut(s) 251, 567, 1184, 1241, 1331, 1373, 1415, 1457
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 1127
SaqAI TTAA 5 cut(s) 116, 263, 627, 693, 1080
SatI GCNGC 2 cut(s) 786, 981
Sau3AI GATC 9 cut(s) 52, 143, 423, 436, 546, 622, 880, 889, 939
Sau96I GGNCC 5 cut(s) 1157, 1175, 1217, 1274, 1292
SchI GAGTC 3 cut(s) 110, 970, 1310
ScrFI CCNGG 3 cut(s) 1068, 1162, 1536
SduI GDGCHC 6 cut(s) 1127, 1200, 1257, 1359, 1401, 1443
SfaNI GCATC 2 cut(s) 56, 634
SfcI CTRYAG 2 cut(s) 105, 1502
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 3 cut(s) 1157, 1175, 1292
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 115, 634
SmoI CTYRAG 2 cut(s) 115, 634
SpeI ACTAGT 1 cut(s) 1030
Sse9I AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
SsiI CCGC 1 cut(s) 788
SspI AATATT 1 cut(s) 1078
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 1 cut(s) 1127
StyD4I CCNGG 3 cut(s) 1066, 1160, 1534
StyI CCWWGG 3 cut(s) 754, 772, 1202
TaaI ACNGT 4 cut(s) 130, 1157, 1365, 1407
TaiI ACGT 1 cut(s) 31
TaqI TCGA 2 cut(s) 55, 779
TasI AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
TatI WGTACW 6 cut(s) 250, 566, 1330, 1372, 1414, 1456
Tru1I TTAA 5 cut(s) 116, 263, 627, 693, 1080
Tru9I TTAA 5 cut(s) 116, 263, 627, 693, 1080
TseFI GTSAC 3 cut(s) 965, 1365, 1407
TseI GCWGC 2 cut(s) 785, 980
Tsp45I GTSAC 3 cut(s) 965, 1365, 1407
TspDTI ATGAA 6 cut(s) 281, 298, 416, 513, 733, 738
TspGWI ACGGA 2 cut(s) 65, 632
Vha464I CTTAAG 1 cut(s) 115
VneI GTGCAC 2 cut(s) 1355, 1397
VpaK11BI GGWCC 3 cut(s) 1157, 1175, 1292
XapI RAATTY 2 cut(s) 72, 798
XceI RCATGY 2 cut(s) 567, 973
XmiI GTMKAC 1 cut(s) 1314
XspI CTAG 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.