Rh1BG310700

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001926
Physical Location & Seq
Forward (+)
44560720 .. 44565439
4720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG310700.1

Sequence Viewer

Length: 1590 bp
ATGGAGAATACTCTCAGGGAGATAATACGTGTGCTTGAACCCTTGCCCACGGATCGAGAAACACGATGGCAAATTCTTCGGGAGTTACAAGGAGCTGTTGAGTCTGTAGAGAGCTTAAGAGGTGCAACTGTGGAGCCATTTGGATCATTTGTCTCAGAACTCTTTACTCGCTGGGGAGACTTGGATGTTTCAATTGAGTTCTCAAAAGGTGCATATATTTCTTCTTATGGAAGAAAGCACACACAAGATGTACTAAAAGATTTAATGAAGGCCATGAGAAAGAGAGGTGGATGGTACAGGTATCAGTTCATACCTAATGCAAGAGTTCCCATTCTGATGGTCAAAAGTAATCTTGGAAACATCTCCTGTGATATTTCAGTCAATAACCTGAAAGGCCAAATGAAATCAAAACTCTTTTTATGGATGAATGATATAGATCCACGCTTTCATGATATGGTTTTACTGGTGAAGGAATGGGCAAAAGCACACAATATCAATAATTCAAAATCTGGGACTTTCAATTCATACTCTCTGAGTTTGCTCGTGATCTTCCATTTTCAGACATGTACACCTGCAATTCTGCCTCCTCTTCAAGACATATATACAGAAACTCTCACGGATGATCTTAAAGGCTTGAGGGATGATGTAGAAAGACGTATTGAAAAAAGTTGTGTTGAGAACATGAGAAGATTTAAACTAAACAAATTGAGAGCTCGGAATAAAAGTTCTTTGTTTGAGCTTTTCATTTCATTCCTTGGGAAGTTCTCTGACCTTGGTTCGAAGGCTGCGGAGTTGGGAATTTGCACATATTCTGGAAAATGGGAAAGAATAGAAAGCAATATGGGATGGTTGCCCAAAACCTATTGTGTATTGGTTGAAGATCCTTTTGATCGGCCAGAAAATGCTGCAAGAGGTGTCAGCCAAAAAGCATTGAAAAGGATCTCTGAAGTTTTTGAGATGAGTTGTGACAGGCTAATCGCAGTTCGTTACAATGCCTCCTTAGCCATTGAGAGTTCCCTTCTTGCCACACTAGTCCGACCACATACGCTACATCTTATTAGCAACCCAGGTTACAATATTAAGCACCACCAAACAACTCATCCACAATATTTCAGGGCTGAGAGCTCACATTCACGAACCCCAAGACATTATAACGGTCCCCCGAAACCAAATGGTCCTTTTGTACGAGAGTTGGTGCCCCCAAGGGAATATAACGGCCCCCCAAAACCAAATGGTAAGGTACAAGAGTTGGTGCCCCAAAGGGATTATAATGGCCCCCCGAAACCAAGTGTTCCTTATGGACGAGAGTTGGTAGACTCATCCCCACAAGGACATGGACATCAACAACTACGAAAGATGGTGCACTCATCCTCACAAGTACAGCCACAAATACAGAAGCTGGTGCACTCACCGTCACAAGTACAACCGCATATACGAAAGTCTGTGCCCTCAACCTCACGAGTACAATCACCACTTCCCTCAACCTCACGAGTACAATCACCACTTCAGAAAGTGAAGCCAGAGAAGGCACCAATGCTACAGGCGTATCAGAAATGGACACCAAAGAAACCAGGAATGCAGACCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

529

Amino Acids

60.4

Weight (kDa)

9.74

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 3 - 139 2.3e-21 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1152, 1269
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 3 cut(s) 1195, 1252, 1528
AccI GTMKAC 1 cut(s) 1314
AciI CCGC 2 cut(s) 788, 1427
AclWI GGATC 5 cut(s) 60, 151, 431, 875, 947
AcoI YGGCCR 1 cut(s) 893
AcsI RAATTY 2 cut(s) 72, 798
AcuI CTGAAG 2 cut(s) 966, 1490
AfaI GTAC 9 cut(s) 252, 296, 568, 1185, 1242, 1380, 1422, 1464, 1494
AflII CTTAAG 1 cut(s) 115
AflIII ACRYGT 2 cut(s) 28, 563
AgsI TTSAA 8 cut(s) 38, 192, 504, 520, 593, 662, 878, 934
AhlI ACTAGT 1 cut(s) 1030
AjnI CCWGG 2 cut(s) 1066, 1570
AloI GAACNNNNNNTCC 2 cut(s) 709, 741
AluBI AGCT 6 cut(s) 95, 114, 713, 739, 1125, 1399
AluI AGCT 6 cut(s) 95, 114, 713, 739, 1125, 1399
Alw21I GWGCWC 4 cut(s) 715, 1127, 1365, 1407
Alw26I GTCTC 2 cut(s) 157, 171
Alw44I GTGCAC 2 cut(s) 1361, 1403
AlwI GGATC 5 cut(s) 60, 151, 431, 875, 947
AlwNI CAGNNNCTG 1 cut(s) 1399
AoxI GGCC 5 cut(s) 270, 394, 893, 1216, 1273
ApaLI GTGCAC 2 cut(s) 1361, 1403
ApeKI GCWGC 2 cut(s) 785, 905
ApoI RAATTY 2 cut(s) 72, 798
AspS9I GGNCC 4 cut(s) 1157, 1175, 1217, 1274
AsuHPI GGTGA 4 cut(s) 478, 1401, 1461, 1491
AsuII TTCGAA 1 cut(s) 779
AvaII GGWCC 2 cut(s) 1157, 1175
BaeGI GKGCMC 5 cut(s) 1200, 1257, 1365, 1407, 1449
BaeI ACNNNNGTAYC 4 cut(s) 1232, 1265, 1529, 1562
BanI GGYRCC 3 cut(s) 1195, 1252, 1528
BanII GRGCYC 2 cut(s) 715, 1127
BauI CACGAG 3 cut(s) 542, 1458, 1488
Bbv12I GWGCWC 4 cut(s) 715, 1127, 1365, 1407
BbvI GCAGC 2 cut(s) 772, 892
BccI CCATC 5 cut(s) 60, 285, 331, 840, 1351
BceAI ACGGC 1 cut(s) 1231
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 2 cut(s) 1068, 1572
BcoDI GTCTC 2 cut(s) 157, 171
BcuI ACTAGT 1 cut(s) 1030
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 2 cut(s) 105, 1538
BfrI CTTAAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 2 cut(s) 786, 906
BlsI GCNGC 2 cut(s) 787, 907
Bme1390I CCNGG 2 cut(s) 1068, 1572
Bme18I GGWCC 2 cut(s) 1157, 1175
BmgT120I GGNCC 4 cut(s) 1157, 1175, 1217, 1274
BmiI GGNNCC 7 cut(s) 135, 1159, 1197, 1219, 1254, 1276, 1530
BmrFI CCNGG 2 cut(s) 1068, 1572
Bpu10I CCTNAGC 1 cut(s) 1000
Bpu14I TTCGAA 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 655
BsaAI YACGTR 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 5 cut(s) 48, 754, 772, 1066, 1202
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bse1I ACTGG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 2 cut(s) 1068, 1572
BseDI CCNNGG 5 cut(s) 48, 754, 772, 1066, 1202
BseGI GGATG 9 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1319, 1367
BseJI GATNNNNATC 1 cut(s) 435
BseMII CTCAG 4 cut(s) 28, 168, 524, 1110
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 5 cut(s) 1200, 1257, 1365, 1407, 1449
BseXI GCAGC 2 cut(s) 772, 892
BseYI CCCAGC 1 cut(s) 171
BshFI GGCC 5 cut(s) 272, 396, 895, 1218, 1275
BshNI GGYRCC 3 cut(s) 1195, 1252, 1528
BsiHKAI GWGCWC 4 cut(s) 715, 1127, 1365, 1407
BslFI GGGAC 2 cut(s) 526, 1143
BsmAI GTCTC 2 cut(s) 157, 171
BsmFI GGGAC 2 cut(s) 526, 1143
BsmI GAATGC 1 cut(s) 1581
BsnI GGCC 5 cut(s) 272, 396, 895, 1218, 1275
Bsp119I TTCGAA 1 cut(s) 779
Bsp1286I GDGCHC 7 cut(s) 715, 1127, 1200, 1257, 1365, 1407, 1449
Bsp1407I TGTACA 1 cut(s) 566
Bsp143I GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BspACI CCGC 2 cut(s) 788, 1427
BspANI GGCC 5 cut(s) 272, 396, 895, 1218, 1275
BspCNI CTCAG 4 cut(s) 27, 167, 525, 1111
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 7 cut(s) 135, 1159, 1197, 1219, 1254, 1276, 1530
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 5 cut(s) 60, 151, 431, 875, 947
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 3 cut(s) 1195, 1252, 1528
BspTI CTTAAG 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 5 cut(s) 48, 754, 772, 1066, 1202
BssMI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BssSI CACGAG 3 cut(s) 542, 1458, 1488
BssT1I CCWWGG 3 cut(s) 754, 772, 1202
Bst2BI CACGAG 3 cut(s) 542, 1458, 1488
Bst2UI CCWGG 2 cut(s) 1068, 1572
Bst4CI ACNGT 3 cut(s) 130, 1157, 1413
Bst6I CTCTTC 1 cut(s) 594
BstAFI CTTAAG 1 cut(s) 115
BstAUI TGTACA 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 779
BstDEI CTNAG 5 cut(s) 14, 154, 533, 1000, 1119
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 9 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1319, 1367
BstKTI GATC 8 cut(s) 55, 146, 439, 549, 625, 883, 892, 942
BstMAI GTCTC 2 cut(s) 157, 171
BstMBI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 2 cut(s) 1068, 1572
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 2 cut(s) 1066, 1570
BstSFI CTRYAG 2 cut(s) 105, 1538
BstSLI GKGCMC 5 cut(s) 1200, 1257, 1365, 1407, 1449
BstV1I GCAGC 2 cut(s) 772, 892
BstX2I RGATCY 3 cut(s) 436, 880, 939
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 3 cut(s) 436, 880, 939
BsuRI GGCC 5 cut(s) 272, 396, 895, 1218, 1275
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 9 cut(s) 190, 296, 429, 625, 646, 851, 1099, 1319, 1367
BveI ACCTGC 1 cut(s) 580
CaiI CAGNNNCTG 1 cut(s) 1399
CciI TCATGA 1 cut(s) 448
Cfr13I GGNCC 4 cut(s) 1157, 1175, 1217, 1274
Csp6I GTAC 9 cut(s) 251, 295, 567, 1184, 1241, 1379, 1421, 1463, 1493
CviAII CATG 5 cut(s) 274, 449, 564, 682, 1334
CviQI GTAC 9 cut(s) 251, 295, 567, 1184, 1241, 1379, 1421, 1463, 1493
DdeI CTNAG 5 cut(s) 14, 154, 533, 1000, 1119
DpnI GATC 8 cut(s) 54, 145, 438, 548, 624, 882, 891, 941
DpnII GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 893
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 2 cut(s) 713, 1125
Eco130I CCWWGG 3 cut(s) 754, 772, 1202
Eco24I GRGCYC 2 cut(s) 715, 1127
Eco47I GGWCC 2 cut(s) 1157, 1175
Eco53kI GAGCTC 2 cut(s) 713, 1125
Eco57I CTGAAG 2 cut(s) 966, 1490
EcoICRI GAGCTC 2 cut(s) 713, 1125
EcoRII CCWGG 2 cut(s) 1066, 1570
EcoT14I CCWWGG 3 cut(s) 754, 772, 1202
EcoT38I GRGCYC 2 cut(s) 715, 1127
ErhI CCWWGG 3 cut(s) 754, 772, 1202
FaeI CATG 5 cut(s) 277, 452, 567, 685, 1337
FalI AAGNNNNNCTT 2 cut(s) 1279, 1311
FaqI GGGAC 2 cut(s) 526, 1143
FatI CATG 5 cut(s) 273, 448, 563, 681, 1333
FblI GTMKAC 1 cut(s) 1314
Fnu4HI GCNGC 2 cut(s) 786, 906
FokI GGATG 9 cut(s) 197, 303, 436, 632, 653, 858, 1086, 1306, 1354
FriOI GRGCYC 2 cut(s) 715, 1127
Fsp4HI GCNGC 2 cut(s) 786, 906
FspBI CTAG 1 cut(s) 1031
GluI GCNGC 2 cut(s) 786, 906
GsaI CCCAGC 1 cut(s) 175
HaeIII GGCC 5 cut(s) 272, 396, 895, 1218, 1275
Hin1II CATG 5 cut(s) 277, 452, 567, 685, 1337
HinfI GANTC 2 cut(s) 101, 1316
HphI GGTGA 4 cut(s) 478, 1401, 1461, 1491
Hpy166II GTNNAC 4 cut(s) 569, 1315, 1363, 1405
Hpy188III TCNNGA 9 cut(s) 56, 80, 449, 544, 593, 813, 1134, 1458, 1488
Hpy8I GTNNAC 4 cut(s) 569, 1315, 1363, 1405
HpyAV CCTTC 5 cut(s) 262, 463, 775, 1028, 1519
HpyCH4III ACNGT 3 cut(s) 130, 1157, 1413
HpyCH4IV ACGT 2 cut(s) 28, 655
HpyCH4V TGCA 9 cut(s) 125, 212, 320, 575, 804, 908, 1363, 1405, 1579
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 5 cut(s) 14, 154, 533, 1000, 1119
HpySE526I ACGT 2 cut(s) 28, 655
Hsp92II CATG 5 cut(s) 277, 452, 567, 685, 1337
Kzo9I GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
LmnI GCTCC 2 cut(s) 92, 133
Lsp1109I GCAGC 2 cut(s) 772, 892
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 2 cut(s) 28, 655
MaeIII GTNAC 5 cut(s) 84, 965, 986, 1070, 1413
MalI GATC 8 cut(s) 54, 145, 438, 548, 624, 882, 891, 941
MboI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
MboII GAAGA 7 cut(s) 68, 213, 243, 541, 581, 699, 890
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 3 cut(s) 436, 880, 939
MhlI GDGCHC 7 cut(s) 715, 1127, 1200, 1257, 1365, 1407, 1449
MluCI AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
MlyI GAGTC 2 cut(s) 110, 1310
MmeI TCCRAC 1 cut(s) 1060
MseI TTAA 5 cut(s) 116, 263, 627, 693, 1080
MslI CAYNNNNRTG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 1068, 1572
MunI CAATTG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 1581
MvaI CCWGG 2 cut(s) 1068, 1572
MwoI GCNNNNNNNGC 1 cut(s) 1001
NdeII GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
NlaIII CATG 5 cut(s) 277, 452, 567, 685, 1337
NlaIV GGNNCC 7 cut(s) 135, 1159, 1197, 1219, 1254, 1276, 1530
NmuCI GTSAC 2 cut(s) 965, 1413
NspI RCATGY 1 cut(s) 567
NspV TTCGAA 1 cut(s) 779
PagI TCATGA 1 cut(s) 448
PaqCI CACCTGC 1 cut(s) 580
PciI ACATGT 1 cut(s) 563
PcsI WCGNNNNNNNCGW 1 cut(s) 61
PctI GAATGC 1 cut(s) 1581
PkrI GCNGC 2 cut(s) 787, 907
PleI GAGTC 2 cut(s) 109, 1310
PpsI GAGTC 2 cut(s) 109, 1310
Ppu21I YACGTR 1 cut(s) 29
PscI ACATGT 1 cut(s) 563
PsiI TTATAA 2 cut(s) 1152, 1269
Psp124BI GAGCTC 2 cut(s) 715, 1127
Psp6I CCWGG 2 cut(s) 1066, 1570
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 2 cut(s) 1066, 1570
PspN4I GGNNCC 7 cut(s) 135, 1159, 1197, 1219, 1254, 1276, 1530
PspPI GGNCC 4 cut(s) 1157, 1175, 1217, 1274
PstNI CAGNNNCTG 1 cut(s) 1399
PsuI RGATCY 3 cut(s) 436, 880, 939
RsaI GTAC 9 cut(s) 252, 296, 568, 1185, 1242, 1380, 1422, 1464, 1494
RsaNI GTAC 9 cut(s) 251, 295, 567, 1184, 1241, 1379, 1421, 1463, 1493
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 715, 1127
SaqAI TTAA 5 cut(s) 116, 263, 627, 693, 1080
SatI GCNGC 2 cut(s) 786, 906
Sau3AI GATC 8 cut(s) 52, 143, 436, 546, 622, 880, 889, 939
Sau96I GGNCC 4 cut(s) 1157, 1175, 1217, 1274
SchI GAGTC 2 cut(s) 110, 1310
ScrFI CCNGG 2 cut(s) 1068, 1572
SduI GDGCHC 7 cut(s) 715, 1127, 1200, 1257, 1365, 1407, 1449
SfcI CTRYAG 2 cut(s) 105, 1538
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 2 cut(s) 1157, 1175
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 115, 634
SmoI CTYRAG 2 cut(s) 115, 634
SpeI ACTAGT 1 cut(s) 1030
Sse9I AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
SsiI CCGC 2 cut(s) 788, 1427
SspI AATATT 2 cut(s) 1078, 1109
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 2 cut(s) 715, 1127
StyD4I CCNGG 2 cut(s) 1066, 1570
StyI CCWWGG 3 cut(s) 754, 772, 1202
TaaI ACNGT 3 cut(s) 130, 1157, 1413
TaiI ACGT 2 cut(s) 31, 658
TaqI TCGA 2 cut(s) 55, 779
TasI AATT 7 cut(s) 72, 192, 499, 520, 576, 704, 798
TatI WGTACW 6 cut(s) 250, 566, 1378, 1420, 1462, 1492
Tru1I TTAA 5 cut(s) 116, 263, 627, 693, 1080
Tru9I TTAA 5 cut(s) 116, 263, 627, 693, 1080
TseFI GTSAC 2 cut(s) 965, 1413
TseI GCWGC 2 cut(s) 785, 905
Tsp45I GTSAC 2 cut(s) 965, 1413
TspDTI ATGAA 8 cut(s) 281, 298, 416, 437, 440, 513, 733, 738
TspGWI ACGGA 2 cut(s) 65, 632
Vha464I CTTAAG 1 cut(s) 115
VneI GTGCAC 2 cut(s) 1361, 1403
VpaK11BI GGWCC 2 cut(s) 1157, 1175
XapI RAATTY 2 cut(s) 72, 798
XceI RCATGY 1 cut(s) 567
XmiI GTMKAC 1 cut(s) 1314
XspI CTAG 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.