MD01G1139300.v1.1

Poly(A) RNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001926
Physical Location & Seq
Forward (+)
24900981 .. 24905501
4521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1139300.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGGAATGTTTAAGAGGTGCAACTGTGGAGCCATTTGGGTCATTTGTGTCTAATCTTTTCACAAGATGGGGGGACCTGGATGTTTCAATTGAGTTTACCAATGGTTCATTTGTTTCATCTAATGGAAAAAAGCAGAAACAGCGTTTACTGGGAGATGTTATGAATGCTTTGAGACAGAAAGGTGGATGGAGGAAGTACCAATTGATACCCAATGCAAGAGTTCCCATTTTAAAGGTTGAAAGTCATATCCAAAATGTCTCCTGTGATATTTCAATCGATAATCTGAAATGTCAAGTGAAGTCCAAACTCTTGCTCTGGATCAGTGAGATGGATACACGCTTCCGCGACATGGTTTTACTGGTAAAGGAATGGGCAAAAGCACACAAAATAAATAATCCAAGAGCCGGGACTTTCAATTCATACTCTCTCACGTTGCTTGTGCTATTCCATTTTCAGACATGTTCACCCGCAATTTTGCCACCTCTTAATGATATATATCCTGGAAATATCATTGATGATCTTAAAGGCTTGAGGGTTGATACAGAGAGACACATCGCAGAAACATGTGGTGCAAATATAGAAAGGATAAAAAGAGCATACAGCATGAGACCAAAAAACAGAAGTTCTTTGTCTGAGCTTTTAGTCTCATTCCTTGGGAAGTTTTCTGACATTAGTACGAGGGCTTCAGAGTTTGGAATTTGCACTTATACTGGACAATGGCACGCCATAGAAAGCAATACGAGATGGTTGCCGAAAACTTCTTATTCCCTATTTATTGAAGATCCTTTTGAGCAGCCAGAAAACATTGCCAGGGCTGTTAGGACCGGTGAACTGACAAGGATATCAGGAGCATTTGCGATGAGCCACCACAGACTTATCTCAACCAATCACAGTTCATTTTCTAACATGCCTGATCAGAGGTCTCTTCTTGCCACCCTAGTCCGACCACAAACTTTACATCTTATTGTAGGACCACCTGTTAGGAATCCGGTATACAATCCTGAGCACCGCCAACCAACTCAGCCACAATGGTTCAGGGCTGAGAGCTCACGTTGGCGAACACCAACCAGCAATAATGGCGCTCGAAAACCAACTCATCCTCAGGTGAACCAACTGGTGCCCTCACCTTCACCAGTGCAACCTCTTCTGGTGCCCATGCTAGTGCACTCACGCTCACAAGTGCAACCTCCTCGGGCGCACAAGCTAGTGTACTCACCCTCACAAGTGCAACCTCAATTGCAGACGACGGAGCCAAAATGGTCTGACAACAGGTCAACCACGGGGCCAAAACAGTATCGCAACGGGTCAACCACCAAAAAACCTCCTCCGCAAACACACCAAGATCGACCACAGCAAATGTGGAGACCAAGATCTGATACAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2

Protein Analysis

462

Amino Acids

52.46

Weight (kDa)

10.01

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 6 - 106 8e-19 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1117, 1150
AccI GTMKAC 1 cut(s) 993
AccII CGCG 1 cut(s) 345
AciI CCGC 4 cut(s) 343, 468, 1009, 1328
AclWI GGATC 2 cut(s) 326, 776
AcsI RAATTY 1 cut(s) 696
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 3 cut(s) 197, 676, 1211
AfiI CCNNNNNNNGG 2 cut(s) 349, 404
AflIII ACRYGT 2 cut(s) 458, 563
AgeI ACCGGT 1 cut(s) 824
AgsI TTSAA 5 cut(s) 87, 239, 273, 415, 779
AhdI GACNNNNNGTC 1 cut(s) 1270
AjnI CCWGG 3 cut(s) 75, 499, 809
AluBI AGCT 4 cut(s) 637, 1047, 1204, 1383
AluI AGCT 4 cut(s) 637, 1047, 1204, 1383
Alw21I GWGCWC 3 cut(s) 1008, 1049, 1167
Alw26I GTCTC 7 cut(s) 166, 262, 541, 601, 649, 927, 1357
Alw44I GTGCAC 1 cut(s) 1163
AlwI GGATC 2 cut(s) 326, 776
Ama87I CYCGRG 1 cut(s) 1191
AoxI GGCC 1 cut(s) 1283
ApaLI GTGCAC 1 cut(s) 1163
ApeKI GCWGC 1 cut(s) 793
ApoI RAATTY 1 cut(s) 696
AsiGI ACCGGT 1 cut(s) 824
AspLEI GCGC 2 cut(s) 1082, 1198
AspS9I GGNCC 4 cut(s) 73, 822, 971, 1283
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 6 cut(s) 456, 839, 1116, 1117, 1122, 1206
AvaI CYCGRG 1 cut(s) 1191
AvaII GGWCC 3 cut(s) 73, 822, 971
AxyI CCTNAGG 1 cut(s) 1101
BaeGI GKGCMC 3 cut(s) 1122, 1155, 1167
BanI GGYRCC 2 cut(s) 1117, 1150
BanII GRGCYC 1 cut(s) 1049
BarI GAAGNNNNNNTAC 2 cut(s) 667, 699
Bbv12I GWGCWC 3 cut(s) 1008, 1049, 1167
BbvI GCAGC 1 cut(s) 805
BccI CCATC 4 cut(s) 60, 180, 322, 738
BcgI CGANNNNNNTGC 4 cut(s) 730, 764, 1172, 1206
BciT130I CCWGG 3 cut(s) 77, 501, 811
BciVI GTATCC 1 cut(s) 325
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 1 cut(s) 406
BcoDI GTCTC 7 cut(s) 166, 262, 541, 601, 649, 927, 1357
BfaI CTAG 3 cut(s) 938, 1160, 1205
BfoI RGCGCY 1 cut(s) 1083
BfuI GTATCC 1 cut(s) 325
BglII AGATCT 1 cut(s) 1370
BisI GCNGC 1 cut(s) 794
BlsI GCNGC 1 cut(s) 795
Bme1390I CCNGG 4 cut(s) 77, 406, 501, 811
Bme18I GGWCC 3 cut(s) 73, 822, 971
BmeRI GACNNNNNGTC 1 cut(s) 1270
BmeT110I CYCGRG 1 cut(s) 1191
BmgT120I GGNCC 4 cut(s) 73, 822, 971, 1283
BmiI GGNNCC 6 cut(s) 30, 74, 1119, 1152, 1251, 1284
BmrFI CCNGG 4 cut(s) 77, 406, 501, 811
BmrI ACTGGG 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 158
Bpu10I CCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 406
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 3 cut(s) 601, 927, 1357
BsaJI CCNNGG 4 cut(s) 652, 810, 1190, 1278
BsaWI WCCGGW 2 cut(s) 824, 988
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 404
Bse118I RCCGGY 1 cut(s) 824
Bse1I ACTGG 5 cut(s) 153, 363, 715, 1119, 1133
Bse21I CCTNAGG 1 cut(s) 1101
Bse3DI GCAATG 1 cut(s) 804
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 3 cut(s) 77, 501, 811
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 4 cut(s) 652, 810, 1190, 1278
BseGI GGATG 3 cut(s) 85, 191, 1096
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 2 cut(s) 349, 404
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 5 cut(s) 624, 993, 1032, 1034, 1115
BseNI ACTGG 5 cut(s) 153, 363, 715, 1119, 1133
BseRI GAGGAG 2 cut(s) 1179, 1314
BseSI GKGCMC 3 cut(s) 1122, 1155, 1167
BseXI GCAGC 1 cut(s) 805
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 1 cut(s) 1285
BshNI GGYRCC 2 cut(s) 1117, 1150
BshTI ACCGGT 1 cut(s) 824
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 3 cut(s) 1008, 1049, 1167
BsiHKCI CYCGRG 1 cut(s) 1191
BsiSI CCGG 3 cut(s) 405, 825, 989
BslFI GGGAC 2 cut(s) 86, 421
BslI CCNNNNNNNGG 2 cut(s) 349, 404
BsmAI GTCTC 7 cut(s) 166, 262, 541, 601, 649, 927, 1357
BsmFI GGGAC 2 cut(s) 86, 421
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 1 cut(s) 1285
Bso31I GGTCTC 3 cut(s) 601, 927, 1357
BsoBI CYCGRG 1 cut(s) 1191
Bsp1286I GDGCHC 5 cut(s) 1008, 1049, 1122, 1155, 1167
Bsp143I GATC 6 cut(s) 318, 517, 781, 913, 1342, 1370
BspACI CCGC 4 cut(s) 343, 468, 1009, 1328
BspANI GGCC 1 cut(s) 1285
BspCNI CTCAG 5 cut(s) 625, 994, 1033, 1033, 1114
BspDI ATCGAT 1 cut(s) 276
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 6 cut(s) 30, 74, 1119, 1152, 1251, 1284
BspPI GGATC 2 cut(s) 326, 776
BspT107I GGYRCC 2 cut(s) 1117, 1150
BspTNI GGTCTC 3 cut(s) 601, 927, 1357
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 1 cut(s) 824
BsrI ACTGG 5 cut(s) 153, 363, 715, 1119, 1133
BssAI RCCGGY 1 cut(s) 824
BssECI CCNNGG 4 cut(s) 652, 810, 1190, 1278
BssMI GATC 6 cut(s) 318, 517, 781, 913, 1342, 1370
BssNAI GTATAC 1 cut(s) 994
BssT1I CCWWGG 1 cut(s) 652
Bst1107I GTATAC 1 cut(s) 994
Bst2UI CCWGG 3 cut(s) 77, 501, 811
Bst4CI ACNGT 3 cut(s) 25, 893, 1293
Bst6I CTCTTC 2 cut(s) 930, 1149
BstC8I GCNNGC 1 cut(s) 723
BstDEI CTNAG 5 cut(s) 633, 1002, 1020, 1041, 1101
BstDSI CCRYGG 1 cut(s) 1278
BstF5I GGATG 3 cut(s) 85, 191, 1096
BstFNI CGCG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 1083
BstHHI GCGC 2 cut(s) 1082, 1198
BstKTI GATC 6 cut(s) 321, 520, 784, 916, 1345, 1373
BstMAI GTCTC 7 cut(s) 166, 262, 541, 601, 649, 927, 1357
BstMBI GATC 6 cut(s) 318, 517, 781, 913, 1342, 1370
BstMWI GCNNNNNNNGC 2 cut(s) 139, 1077
BstNI CCWGG 3 cut(s) 77, 501, 811
BstNSI RCATGY 3 cut(s) 462, 567, 910
BstSCI CCNGG 4 cut(s) 75, 404, 499, 809
BstSLI GKGCMC 3 cut(s) 1122, 1155, 1167
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 1 cut(s) 805
BstX2I RGATCY 2 cut(s) 781, 1370
BstYI RGATCY 2 cut(s) 781, 1370
BstZ17I GTATAC 1 cut(s) 994
Bsu15I ATCGAT 1 cut(s) 276
Bsu36I CCTNAGG 1 cut(s) 1101
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 1 cut(s) 1285
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 1278
BtgZI GCGATG 2 cut(s) 538, 872
BtsCI GGATG 3 cut(s) 85, 191, 1096
BtsIMutI CAGTG 2 cut(s) 328, 1140
Cac8I GCNNGC 1 cut(s) 723
CfoI GCGC 2 cut(s) 1082, 1198
Cfr10I RCCGGY 1 cut(s) 824
Cfr13I GGNCC 4 cut(s) 73, 822, 971, 1283
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 3 cut(s) 196, 675, 1210
CspAI ACCGGT 1 cut(s) 824
CviAII CATG 6 cut(s) 349, 459, 564, 604, 907, 1156
CviQI GTAC 3 cut(s) 196, 675, 1210
DdeI CTNAG 5 cut(s) 633, 1002, 1020, 1041, 1101
DpnI GATC 6 cut(s) 320, 519, 783, 915, 1344, 1372
DpnII GATC 6 cut(s) 318, 517, 781, 913, 1342, 1370
DraI TTTAAA 1 cut(s) 231
DriI GACNNNNNGTC 1 cut(s) 1270
Eam1104I CTCTTC 2 cut(s) 930, 1149
Eam1105I GACNNNNNGTC 1 cut(s) 1270
EarI CTCTTC 2 cut(s) 930, 1149
Ecl136II GAGCTC 1 cut(s) 1047
Eco130I CCWWGG 1 cut(s) 652
Eco24I GRGCYC 1 cut(s) 1049
Eco31I GGTCTC 3 cut(s) 601, 927, 1357
Eco32I GATATC 1 cut(s) 843
Eco47I GGWCC 3 cut(s) 73, 822, 971
Eco53kI GAGCTC 1 cut(s) 1047
Eco57I CTGAAG 1 cut(s) 669
Eco81I CCTNAGG 1 cut(s) 1101
Eco88I CYCGRG 1 cut(s) 1191
EcoICRI GAGCTC 1 cut(s) 1047
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 75, 499, 809
EcoRV GATATC 1 cut(s) 843
EcoT14I CCWWGG 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 1049
ErhI CCWWGG 1 cut(s) 652
FaeI CATG 6 cut(s) 352, 462, 567, 607, 910, 1159
FaqI GGGAC 2 cut(s) 86, 421
FatI CATG 6 cut(s) 348, 458, 563, 603, 906, 1155
FauI CCCGC 1 cut(s) 475
FbaI TGATCA 1 cut(s) 913
FblI GTMKAC 1 cut(s) 993
Fnu4HI GCNGC 1 cut(s) 794
FokI GGATG 3 cut(s) 92, 198, 1083
FriOI GRGCYC 1 cut(s) 1049
Fsp4HI GCNGC 1 cut(s) 794
FspBI CTAG 3 cut(s) 938, 1160, 1205
GlaI GCGC 2 cut(s) 1081, 1197
GluI GCNGC 1 cut(s) 794
HaeII RGCGCY 1 cut(s) 1083
HaeIII GGCC 1 cut(s) 1285
HapII CCGG 3 cut(s) 405, 825, 989
HhaI GCGC 2 cut(s) 1082, 1198
Hin1II CATG 6 cut(s) 352, 462, 567, 607, 910, 1159
Hin6I GCGC 2 cut(s) 1080, 1196
HinP1I GCGC 2 cut(s) 1080, 1196
HincII GTYRAC 2 cut(s) 1275, 1308
HindII GTYRAC 2 cut(s) 1275, 1308
HinfI GANTC 1 cut(s) 985
HpaII CCGG 3 cut(s) 405, 825, 989
HphI GGTGA 6 cut(s) 456, 839, 1116, 1117, 1122, 1206
Hpy188I TCNGA 9 cut(s) 285, 456, 634, 667, 688, 918, 944, 1264, 1375
Hpy188III TCNNGA 3 cut(s) 316, 846, 1001
Hpy99I CGWCG 1 cut(s) 1249
HpyAV CCTTC 1 cut(s) 1137
HpyCH4III ACNGT 3 cut(s) 25, 893, 1293
HpyCH4IV ACGT 2 cut(s) 431, 1051
HpyCH4V TGCA 9 cut(s) 20, 215, 572, 702, 1138, 1165, 1183, 1228, 1240
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 1077
HpyF3I CTNAG 5 cut(s) 633, 1002, 1020, 1041, 1101
HpySE526I ACGT 2 cut(s) 431, 1051
Hsp92II CATG 6 cut(s) 352, 462, 567, 607, 910, 1159
HspAI GCGC 2 cut(s) 1080, 1196
Ksp22I TGATCA 1 cut(s) 913
Kzo9I GATC 6 cut(s) 318, 517, 781, 913, 1342, 1370
LmnI GCTCC 3 cut(s) 28, 848, 1249
Lsp1109I GCAGC 1 cut(s) 805
MaeI CTAG 3 cut(s) 938, 1160, 1205
MaeII ACGT 2 cut(s) 431, 1051
MalI GATC 6 cut(s) 320, 519, 783, 915, 1344, 1372
MboI GATC 6 cut(s) 318, 517, 781, 913, 1342, 1370
MboII GAAGA 3 cut(s) 791, 917, 1136
MfeI CAATTG 3 cut(s) 87, 200, 1235
MflI RGATCY 2 cut(s) 781, 1370
MhlI GDGCHC 5 cut(s) 1008, 1049, 1122, 1155, 1167
MluCI AATT 6 cut(s) 87, 200, 415, 471, 696, 1235
MmeI TCCRAC 1 cut(s) 967
MseI TTAA 4 cut(s) 11, 230, 486, 522
MslI CAYNNNNRTG 1 cut(s) 1160
MspI CCGG 3 cut(s) 405, 825, 989
MspR9I CCNGG 4 cut(s) 77, 406, 501, 811
MunI CAATTG 3 cut(s) 87, 200, 1235
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 3 cut(s) 77, 501, 811
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 2 cut(s) 139, 1077
NciI CCSGG 1 cut(s) 406
NdeII GATC 6 cut(s) 318, 517, 781, 913, 1342, 1370
NlaIII CATG 6 cut(s) 352, 462, 567, 607, 910, 1159
NlaIV GGNNCC 6 cut(s) 30, 74, 1119, 1152, 1251, 1284
NspI RCATGY 3 cut(s) 462, 567, 910
PciI ACATGT 2 cut(s) 458, 563
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 985
PfoI TCCNGGA 1 cut(s) 499
PinAI ACCGGT 1 cut(s) 824
PkrI GCNGC 1 cut(s) 795
PpuMI RGGWCCY 1 cut(s) 73
PscI ACATGT 2 cut(s) 458, 563
Psp124BI GAGCTC 1 cut(s) 1049
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 3 cut(s) 75, 499, 809
PspGI CCWGG 3 cut(s) 75, 499, 809
PspN4I GGNNCC 6 cut(s) 30, 74, 1119, 1152, 1251, 1284
PspPI GGNCC 4 cut(s) 73, 822, 971, 1283
PspPPI RGGWCCY 1 cut(s) 73
PsuI RGATCY 2 cut(s) 781, 1370
RsaI GTAC 3 cut(s) 197, 676, 1211
RsaNI GTAC 3 cut(s) 196, 675, 1210
RseI CAYNNNNRTG 1 cut(s) 1160
SacI GAGCTC 1 cut(s) 1049
SaqAI TTAA 4 cut(s) 11, 230, 486, 522
SatI GCNGC 1 cut(s) 794
Sau3AI GATC 6 cut(s) 318, 517, 781, 913, 1342, 1370
Sau96I GGNCC 4 cut(s) 73, 822, 971, 1283
ScrFI CCNGG 4 cut(s) 77, 406, 501, 811
SduI GDGCHC 5 cut(s) 1008, 1049, 1122, 1155, 1167
SinI GGWCC 3 cut(s) 73, 822, 971
SmiMI CAYNNNNRTG 1 cut(s) 1160
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
Sse9I AATT 6 cut(s) 87, 200, 415, 471, 696, 1235
SsiI CCGC 4 cut(s) 343, 468, 1009, 1328
SspMI CTAG 3 cut(s) 938, 1160, 1205
SstI GAGCTC 1 cut(s) 1049
StyD4I CCNGG 4 cut(s) 75, 404, 499, 809
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 3 cut(s) 25, 893, 1293
TaiI ACGT 2 cut(s) 434, 1054
TaqI TCGA 3 cut(s) 276, 1084, 1345
TasI AATT 6 cut(s) 87, 200, 415, 471, 696, 1235
TatI WGTACW 1 cut(s) 1209
TfiI GAWTC 1 cut(s) 985
Tru1I TTAA 4 cut(s) 11, 230, 486, 522
Tru9I TTAA 4 cut(s) 11, 230, 486, 522
TscAI CASTG 2 cut(s) 328, 1140
TseI GCWGC 1 cut(s) 793
TspDTI ATGAA 5 cut(s) 96, 105, 176, 408, 885
TspGWI ACGGA 1 cut(s) 1262
TspRI CASTG 2 cut(s) 328, 1140
VneI GTGCAC 1 cut(s) 1163
VpaK11BI GGWCC 3 cut(s) 73, 822, 971
XapI RAATTY 1 cut(s) 696
XceI RCATGY 3 cut(s) 462, 567, 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 1356
XmiI GTMKAC 1 cut(s) 993
XspI CTAG 3 cut(s) 938, 1160, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.