FvH4_7g24581
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003287
Physical Location & Seq
Reverse (-)
18962939 .. 18964073
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24581.t1

Sequence Viewer

Length: 846 bp
ATGATAGCAGCAAAGTTACCAAAACCTTTTTGGTTTCATGCAGTTGCTCACTCTGCATTTCTTATAAATAGGATGCCTTGTCAGGTTCTTAATATGGAGTCTCCTTATTCAAAGTTGTTTGGTTCAGCTCCTGATGTTTCTCAGTTTCGGATTTTTGGTTCAGCGTGTTATCCTTTTCTTCGTCCATATTCAGCAGATAAACTTGATCCTAGAAGTATCAGATGTGTTTTTTTGGGATATGCACTTGGTTTTAAAGGAGTATTCTGTTACAGTATAGAGAAGAATAAGCTCTGGATGTGGCGTCATGTGGTGTTTGATGAATCAGTGTTTCCATATCATCTTGTTGGTCAACTTTTGCATTCTCAGTCCTCTTCAGGTACTTCCAGACCTAGCTCATCTGTTTTTCTTAAATCAGTTTCTCAGTCCTCTCCTATTTCCTCAGTTGATTCTGATCAGGGGGGTAATACTGGCGGTATTGGGAATAATTCTCCTGTTGATATTAATCATACTGATGGTGTTGTTTTGAATTCTGATAATGGAGTTGCTGAAAATTCTGGGAATACTAGTGTTGAAGTAAATCCAAGTAATGTTGAAAATATTGATAATACTGTTGCTGCAGTAGAGAATGGTACAGGGGTTGGAAATATTGTTAATATTGTTGATGCAGTAGAGAATGGTACAAGTTACAATCCATATGCTTTTCCTGAACATGGCTTTGTTGAAAATCCTATTGTTTCTAGTAATGGTTTAAATGCAGCAGAATATTCTCTTTCTCATGAGTTAACAAACTACAACAAGCTCAGAGTAGTCACAGAATCAAGAGCCGAAGCTCTGATACCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

282

Amino Acids

30.48

Weight (kDa)

5.58

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 54 - 113 2.4e-16 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 65
AciI CCGC 1 cut(s) 471
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 2 cut(s) 526, 550
AcuI CTGAAG 1 cut(s) 357
AcyI GRCGYC 1 cut(s) 301
AfaI GTAC 3 cut(s) 379, 631, 679
AfiI CCNNNNNNNGG 1 cut(s) 710
AgsI TTSAA 5 cut(s) 111, 526, 572, 593, 722
AhlI ACTAGT 1 cut(s) 563
AloI GAACNNNNNNTCC 2 cut(s) 142, 174
AluBI AGCT 5 cut(s) 128, 289, 393, 799, 830
AluI AGCT 5 cut(s) 128, 289, 393, 799, 830
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 1 cut(s) 200
AlwNI CAGNNNCTG 1 cut(s) 131
ApeKI GCWGC 3 cut(s) 8, 614, 755
ApoI RAATTY 2 cut(s) 526, 550
AseI ATTAAT 1 cut(s) 501
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BarI GAAGNNNNNNTAC 1 cut(s) 819
BbvI GCAGC 3 cut(s) 20, 601, 767
BccI CCATC 1 cut(s) 506
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 563
BfaI CTAG 4 cut(s) 210, 390, 564, 738
BfmI CTRYAG 1 cut(s) 615
BisI GCNGC 3 cut(s) 9, 615, 756
BlsI GCNGC 3 cut(s) 10, 616, 757
BmsI GCATC 2 cut(s) 63, 652
BsaBI GATNNNNATC 2 cut(s) 450, 501
BsaHI GRCGYC 1 cut(s) 301
Bsc4I CCNNNNNNNGG 1 cut(s) 710
Bse1I ACTGG 1 cut(s) 472
Bse8I GATNNNNATC 2 cut(s) 450, 501
BseGI GGATG 2 cut(s) 78, 300
BseJI GATNNNNATC 2 cut(s) 450, 501
BseLI CCNNNNNNNGG 1 cut(s) 710
BseMII CTCAG 5 cut(s) 155, 377, 434, 453, 814
BseNI ACTGG 1 cut(s) 472
BseXI GCAGC 3 cut(s) 20, 601, 767
BslI CCNNNNNNNGG 1 cut(s) 710
BsmAI GTCTC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 358
Bsp143I GATC 2 cut(s) 205, 451
BspACI CCGC 1 cut(s) 471
BspCNI CTCAG 5 cut(s) 154, 376, 433, 452, 813
BspHI TCATGA 1 cut(s) 775
BspMAI CTGCAG 1 cut(s) 619
BspPI GGATC 1 cut(s) 200
BsrI ACTGG 1 cut(s) 472
BssMI GATC 2 cut(s) 205, 451
BssNI GRCGYC 1 cut(s) 301
Bst4CI ACNGT 2 cut(s) 272, 610
Bst6I CTCTTC 1 cut(s) 376
BstACI GRCGYC 1 cut(s) 301
BstDEI CTNAG 5 cut(s) 141, 363, 420, 439, 800
BstF5I GGATG 2 cut(s) 78, 300
BstKTI GATC 2 cut(s) 208, 454
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 2 cut(s) 205, 451
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstSFI CTRYAG 1 cut(s) 615
BstV1I GCAGC 3 cut(s) 20, 601, 767
BtsCI GGATG 2 cut(s) 78, 300
BtsIMutI CAGTG 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 131
CciI TCATGA 1 cut(s) 775
CseI GACGC 1 cut(s) 290
Csp6I GTAC 3 cut(s) 378, 630, 678
CviAII CATG 5 cut(s) 38, 305, 710, 776, 840
CviJI RGCY 7 cut(s) 128, 289, 393, 714, 799, 824, 830
CviKI_1 RGCY 7 cut(s) 128, 289, 393, 714, 799, 824, 830
CviQI GTAC 3 cut(s) 378, 630, 678
DdeI CTNAG 5 cut(s) 141, 363, 420, 439, 800
DpnI GATC 2 cut(s) 207, 453
DpnII GATC 2 cut(s) 205, 451
DraI TTTAAA 2 cut(s) 253, 750
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 357
EcoRI GAATTC 1 cut(s) 526
FaeI CATG 5 cut(s) 41, 308, 713, 779, 843
FatI CATG 5 cut(s) 37, 304, 709, 775, 839
FauNDI CATATG 1 cut(s) 694
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 3 cut(s) 9, 615, 756
FokI GGATG 2 cut(s) 85, 307
Fsp4HI GCNGC 3 cut(s) 9, 615, 756
FspBI CTAG 4 cut(s) 210, 390, 564, 738
GluI GCNGC 3 cut(s) 9, 615, 756
HgaI GACGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 301
Hin1II CATG 5 cut(s) 41, 308, 713, 779, 843
HincII GTYRAC 2 cut(s) 350, 783
HindII GTYRAC 2 cut(s) 350, 783
HinfI GANTC 4 cut(s) 98, 320, 446, 815
HpaI GTTAAC 1 cut(s) 783
Hpy166II GTNNAC 2 cut(s) 350, 783
Hpy188I TCNGA 6 cut(s) 150, 221, 451, 532, 803, 834
Hpy188III TCNNGA 6 cut(s) 131, 292, 384, 704, 776, 819
Hpy8I GTNNAC 2 cut(s) 350, 783
HpyCH4III ACNGT 2 cut(s) 272, 610
HpyCH4V TGCA 7 cut(s) 41, 56, 242, 358, 617, 665, 755
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 5 cut(s) 141, 363, 420, 439, 800
Hsp92I GRCGYC 1 cut(s) 301
Hsp92II CATG 5 cut(s) 41, 308, 713, 779, 843
Ksp22I TGATCA 1 cut(s) 451
KspAI GTTAAC 1 cut(s) 783
Kzo9I GATC 2 cut(s) 205, 451
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 3 cut(s) 20, 601, 767
LweI GCATC 2 cut(s) 63, 652
MaeI CTAG 4 cut(s) 210, 390, 564, 738
MaeIII GTNAC 4 cut(s) 15, 266, 683, 808
MalI GATC 2 cut(s) 207, 453
MboI GATC 2 cut(s) 205, 451
MboII GAAGA 3 cut(s) 170, 292, 363
MluCI AATT 3 cut(s) 484, 526, 550
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 619
MnlI CCTC 3 cut(s) 379, 436, 448
MseI TTAA 7 cut(s) 90, 252, 408, 501, 651, 749, 782
MslI CAYNNNNRTG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 358
MwoI GCNNNNNNNGC 1 cut(s) 53
NdeI CATATG 1 cut(s) 694
NdeII GATC 2 cut(s) 205, 451
NlaIII CATG 5 cut(s) 41, 308, 713, 779, 843
NmuCI GTSAC 1 cut(s) 808
PagI TCATGA 1 cut(s) 775
PctI GAATGC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 320, 446, 815
PkrI GCNGC 3 cut(s) 10, 616, 757
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 501
PsiI TTATAA 1 cut(s) 65
PstI CTGCAG 1 cut(s) 619
PstNI CAGNNNCTG 1 cut(s) 131
RsaI GTAC 3 cut(s) 379, 631, 679
RsaNI GTAC 3 cut(s) 378, 630, 678
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 7 cut(s) 90, 252, 408, 501, 651, 749, 782
SatI GCNGC 3 cut(s) 9, 615, 756
Sau3AI GATC 2 cut(s) 205, 451
SchI GAGTC 1 cut(s) 107
SetI ASST 9 cut(s) 28, 87, 130, 291, 379, 391, 395, 801, 832
SfaNI GCATC 2 cut(s) 63, 652
SfcI CTRYAG 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 510
SpeI ACTAGT 1 cut(s) 563
Sse9I AATT 3 cut(s) 484, 526, 550
SsiI CCGC 1 cut(s) 471
SspI AATATT 4 cut(s) 598, 646, 655, 764
SspMI CTAG 4 cut(s) 210, 390, 564, 738
TaaI ACNGT 2 cut(s) 272, 610
TasI AATT 3 cut(s) 484, 526, 550
TfiI GAWTC 3 cut(s) 320, 446, 815
Tru1I TTAA 7 cut(s) 90, 252, 408, 501, 651, 749, 782
Tru9I TTAA 7 cut(s) 90, 252, 408, 501, 651, 749, 782
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 3 cut(s) 8, 614, 755
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 2 cut(s) 26, 333
TspRI CASTG 1 cut(s) 330
VspI ATTAAT 1 cut(s) 501
XapI RAATTY 2 cut(s) 526, 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 27
XspI CTAG 4 cut(s) 210, 390, 564, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.