RchiOBHm_Chr1g0313681
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003287
Physical Location & Seq
Forward (+)
778129 .. 779123
995 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54449

Sequence Viewer

Length: 789 bp
ATGTCTTCCCCTTATATTGAGTTGTTTGGATCTGCACCTGATCTTAAGTTTCTCAGAATTTTTGGAACTGCCTGTTATCCATATCTTCGACCTTATTCACAGGATAAGTTGGATCCTAGAACTGTTCAGTGTGTTTTTCTTGGCTATGACTTGGGATATAAGGGTGTGTTTTGCTATAACATTGAGAAGAATAAATTGTGGATGAGCAGACATGTTGTGCATGATGAAACCATTTTTCCATACCATTTCCATTCACCTAGTTCTTCTTCCAAACAGTGTTCTTCTTCTAGTACTTCCTTTCAATATTCTTCACCATCTATGTCCACACCTCAGTCCCATGTGTTGCCTGTCCTGCATTCTAATCAGTTACAAATTGTGCTTCCCTTTGCGTCTTCCTCTCAGCCCTCAACTGGTTCCCCACCTGTGGCTGTGGGGGGGGGGGATAATGAGATTGGTTCTAGTGGGTCTAATGAGTCTTCATCTTCATCTGGTTCCTCTTTTGGTTCTGCAAGGTCCTCTGATGATGTTGTTGGTGTAAACAATGGACATAATAATGTTGATTTGAGTCATGAAAGTGAAGAAGAGAATGGTGTGATGGTGCAGTCTAGTGAAGTTCACCCTGATATTCCCAATGGTCATCTTCATGACACTTTAGATGGCCAAAGTATAAATGCTGGTCTAAATATTACTGATGATCTTCCACATGAGCTGCAATTTATTGAAGATACTGCTAGAGATTATAACAGTCATTCCATGTTAACAAGAGCAAAGAATGGTATCTTTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

262

Amino Acids

28.54

Weight (kDa)

5.31

Isoelectric Point (pI)

72.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 24 - 83 1.1e-17 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 741
AclWI GGATC 3 cut(s) 37, 107, 120
AcoI YGGCCR 1 cut(s) 658
AcsI RAATTY 1 cut(s) 57
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 2 cut(s) 410, 424
AflII CTTAAG 1 cut(s) 44
AflIII ACRYGT 1 cut(s) 211
AgsI TTSAA 2 cut(s) 302, 722
AluBI AGCT 1 cut(s) 709
AluI AGCT 1 cut(s) 709
AlwI GGATC 3 cut(s) 37, 107, 120
AoxI GGCC 1 cut(s) 658
ApeKI GCWGC 1 cut(s) 709
ApoI RAATTY 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 513
AsuHPI GGTGA 3 cut(s) 246, 303, 608
AvaII GGWCC 1 cut(s) 513
BalI TGGCCA 1 cut(s) 660
BamHI GGATCC 1 cut(s) 112
BbsI GAAGAC 2 cut(s) 384, 468
BbvI GCAGC 1 cut(s) 696
BccI CCATC 3 cut(s) 322, 589, 650
BfaI CTAG 6 cut(s) 117, 258, 288, 459, 606, 732
BfrI CTTAAG 1 cut(s) 44
BisI GCNGC 1 cut(s) 710
BlsI GCNGC 1 cut(s) 711
BmcAI AGTACT 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 513
BmiI GGNNCC 3 cut(s) 114, 415, 493
BpiI GAAGAC 2 cut(s) 384, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 410, 424
Bse1I ACTGG 1 cut(s) 415
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 2 cut(s) 410, 424
BseMII CTCAG 3 cut(s) 67, 344, 413
BseNI ACTGG 1 cut(s) 415
BseXI GCAGC 1 cut(s) 696
BsgI GTGCAG 2 cut(s) 18, 620
BshFI GGCC 1 cut(s) 660
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 2 cut(s) 410, 424
BsmFI GGGAC 1 cut(s) 319
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 1 cut(s) 660
Bsp143I GATC 4 cut(s) 29, 40, 112, 694
BspANI GGCC 1 cut(s) 660
BspCNI CTCAG 3 cut(s) 66, 343, 412
BspHI TCATGA 2 cut(s) 568, 643
BspLI GGNNCC 3 cut(s) 114, 415, 493
BspPI GGATC 3 cut(s) 37, 107, 120
BspTI CTTAAG 1 cut(s) 44
BsrI ACTGG 1 cut(s) 415
BssMI GATC 4 cut(s) 29, 40, 112, 694
Bst4CI ACNGT 3 cut(s) 124, 276, 746
Bst6I CTCTTC 1 cut(s) 576
BstAFI CTTAAG 1 cut(s) 44
BstDEI CTNAG 3 cut(s) 53, 330, 399
BstF5I GGATG 1 cut(s) 207
BstKTI GATC 4 cut(s) 32, 43, 115, 697
BstMBI GATC 4 cut(s) 29, 40, 112, 694
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstNSI RCATGY 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 696
BstV2I GAAGAC 2 cut(s) 384, 468
BstX2I RGATCY 2 cut(s) 29, 112
BstYI RGATCY 2 cut(s) 29, 112
BsuRI GGCC 1 cut(s) 660
BtsCI GGATG 1 cut(s) 207
BtsIMutI CAGTG 2 cut(s) 134, 281
CciI TCATGA 2 cut(s) 568, 643
Cfr13I GGNCC 1 cut(s) 513
CseI GACGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 291
CviAII CATG 7 cut(s) 212, 221, 338, 569, 644, 704, 754
CviJI RGCY 5 cut(s) 144, 403, 428, 660, 709
CviKI_1 RGCY 5 cut(s) 144, 403, 428, 660, 709
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 3 cut(s) 53, 330, 399
DpnI GATC 4 cut(s) 31, 42, 114, 696
DpnII GATC 4 cut(s) 29, 40, 112, 694
EaeI YGGCCR 1 cut(s) 658
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 513
EcoO109I RGGNCCY 1 cut(s) 513
FaeI CATG 7 cut(s) 215, 224, 341, 572, 647, 707, 757
FaqI GGGAC 1 cut(s) 319
FatI CATG 7 cut(s) 211, 220, 337, 568, 643, 703, 753
Fnu4HI GCNGC 1 cut(s) 710
FokI GGATG 1 cut(s) 214
Fsp4HI GCNGC 1 cut(s) 710
FspBI CTAG 6 cut(s) 117, 258, 288, 459, 606, 732
GluI GCNGC 1 cut(s) 710
HaeIII GGCC 1 cut(s) 660
HgaI GACGC 1 cut(s) 378
Hin1II CATG 7 cut(s) 215, 224, 341, 572, 647, 707, 757
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HinfI GANTC 2 cut(s) 473, 565
HpaI GTTAAC 1 cut(s) 759
HphI GGTGA 3 cut(s) 246, 303, 608
Hpy166II GTNNAC 4 cut(s) 324, 538, 616, 759
Hpy188I TCNGA 2 cut(s) 56, 520
Hpy188III TCNNGA 2 cut(s) 569, 644
Hpy8I GTNNAC 4 cut(s) 324, 538, 616, 759
HpyCH4III ACNGT 3 cut(s) 124, 276, 746
HpyCH4V TGCA 6 cut(s) 35, 220, 355, 509, 601, 712
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 3 cut(s) 53, 330, 399
Hsp92II CATG 7 cut(s) 215, 224, 341, 572, 647, 707, 757
KspAI GTTAAC 1 cut(s) 759
Kzo9I GATC 4 cut(s) 29, 40, 112, 694
Lsp1109I GCAGC 1 cut(s) 696
MaeI CTAG 6 cut(s) 117, 258, 288, 459, 606, 732
MaeIII GTNAC 1 cut(s) 366
MalI GATC 4 cut(s) 31, 42, 114, 696
MboI GATC 4 cut(s) 29, 40, 112, 694
MflI RGATCY 2 cut(s) 29, 112
MlsI TGGCCA 1 cut(s) 660
MluCI AATT 4 cut(s) 57, 194, 372, 713
MluNI TGGCCA 1 cut(s) 660
MlyI GAGTC 2 cut(s) 482, 574
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 5 cut(s) 339, 406, 415, 505, 526
Mox20I TGGCCA 1 cut(s) 660
MscI TGGCCA 1 cut(s) 660
MseI TTAA 3 cut(s) 45, 758, 783
MslI CAYNNNNRTG 3 cut(s) 552, 573, 642
Msp20I TGGCCA 1 cut(s) 660
MspCI CTTAAG 1 cut(s) 44
Mva1269I GAATGC 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 352
NdeII GATC 4 cut(s) 29, 40, 112, 694
NlaIII CATG 7 cut(s) 215, 224, 341, 572, 647, 707, 757
NlaIV GGNNCC 3 cut(s) 114, 415, 493
NspI RCATGY 1 cut(s) 215
PagI TCATGA 2 cut(s) 568, 643
PciI ACATGT 1 cut(s) 211
PctI GAATGC 1 cut(s) 355
PkrI GCNGC 1 cut(s) 711
PleI GAGTC 2 cut(s) 481, 573
PpsI GAGTC 2 cut(s) 481, 573
PpuMI RGGWCCY 1 cut(s) 513
PscI ACATGT 1 cut(s) 211
PsiI TTATAA 1 cut(s) 741
Psp5II RGGWCCY 1 cut(s) 513
PspN4I GGNNCC 3 cut(s) 114, 415, 493
PspPI GGNCC 1 cut(s) 513
PspPPI RGGWCCY 1 cut(s) 513
PsuI RGATCY 2 cut(s) 29, 112
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
RseI CAYNNNNRTG 3 cut(s) 552, 573, 642
SaqAI TTAA 3 cut(s) 45, 758, 783
SatI GCNGC 1 cut(s) 710
Sau3AI GATC 4 cut(s) 29, 40, 112, 694
Sau96I GGNCC 1 cut(s) 513
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 2 cut(s) 482, 574
SetI ASST 7 cut(s) 40, 94, 259, 331, 424, 515, 711
SinI GGWCC 1 cut(s) 513
SmiMI CAYNNNNRTG 3 cut(s) 552, 573, 642
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 4 cut(s) 57, 194, 372, 713
SspI AATATT 2 cut(s) 305, 685
SspMI CTAG 6 cut(s) 117, 258, 288, 459, 606, 732
TaaI ACNGT 3 cut(s) 124, 276, 746
TaqI TCGA 1 cut(s) 88
TasI AATT 4 cut(s) 57, 194, 372, 713
TatI WGTACW 1 cut(s) 290
Tru1I TTAA 3 cut(s) 45, 758, 783
Tru9I TTAA 3 cut(s) 45, 758, 783
TscAI CASTG 2 cut(s) 134, 281
TseI GCWGC 1 cut(s) 709
TspDTI ATGAA 5 cut(s) 240, 468, 474, 585, 632
TspRI CASTG 2 cut(s) 134, 281
Vha464I CTTAAG 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 513
XapI RAATTY 1 cut(s) 57
XceI RCATGY 1 cut(s) 215
XspI CTAG 6 cut(s) 117, 258, 288, 459, 606, 732
ZrmI AGTACT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.