Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0004037

Physical Location & Seq

Genomic Coordinates Forward (+)

18989222 .. 18992735

Gene Structure 3514 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g24620.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGCAGATACCTCGTAGAAAAACACCCTTCTCTGCTTCCCCTGAAGCTTTCACAATGAAGAGCCGCAAGAACAACAACCTACCAATCTTCGTCGTCGTCTTCTCGGTTTTTCTGTTCGGGATTTTCATGTACAATGAAGACGTCAAGTCCATCGCGGAATTCCCATTCTCTTCTACCCCAAAAGCGCAAGAACTCCAAGAAATGCAAGAAACGAAACAGAGCTCCTCAGACTCTGTTTCGAAACAGAGCGACTCTGTTTCCGAAATCCAGAACAGGGTTTCTCTAAGCGCAACTGCAAGAACGCAATTGGAAGAAACCCAGTTGGAGAATCCAGTTTCTGAAGAGACCCAAGAACCTGTGGAGCTGAAAACGATGATGGAAGAGAAAGAAGAAGACTTGGACAAAAAGATTGAGGTGAATGAAGAAGATGATACGGAAGAGATTGAATTGCCTCCTGAAGATTGTGACTTGTTTAATGGGGATTGGGTTTTCGATCCTGTGACTCATCCTTTGTATAAAGAAGACGAATGTGAGTTCTTGACGGCTCAAGTAACTTGTATGAGGAATGGAAGAAAGGACTCTCTGTATCAACATTGGAGATGGCAGCCCAGAGATTGTAATCTTCCAAAGTTCAATGCAAGATTGTTGCTAGAGAAACTGAGAAACAAGAGGCTCATGTTTGTGGGGGATTCTTTGAATAGAAACCAGTGGGAATCCATGATATGTTTTGCTCAGTCTGTAATTCCCCCTGGGAGAAAGAGCTTGGTGAAGAATGGATCTCTCTCTGTTTTCACAATTAAGGACTATAATGCTACAGTGGAGTTCTACTGGGCACCATTTCTGGTGGAGTCAAATTCAGATGATCCATTGATGCACAGCATACAAAACCGTATCATTATGCCCAAGTCGATCAAGAAGCATGGTAAAAATTGGAAGGATGTGGATTACCTAGTTTTCAACACATACATTTGGTGGATGAACACGTTCAACATGAAAATTCTACGAGGATCGTTTGATCAAGGCGCCACCGAGTATTATGAGATTGACCGGCCGTTGGCTTACCGGAAAGTTTTGACGACATGGGCTAAATGGGTGGACAAAAATATTGACCCCAATCGGACCACTGTCTTCTTCACCAGCATGTCTCCTCTTCACATCAAGAGCTTGGATTGGGACAACCCGGATGGCATAAAATGTGCCAAAGAGACCACTCCAGTCCTGAACAGAACGACACCGTTGGAAGTGGGCACAGACAGAAGGCTCTTTGTGATTTCAAGCGACATAACAAAAGCAATGAAAGTGCCTGTTTACTTTCTCAACATTACTACTCTGTCTGAGTTTCGTAAAGATGCACATACCTCAGTCCATACCATCAGGCAAGGCAAGATGTTGACGCCGGAGCAGCAAGCTGACCCGGAGACCTACGCCGACTGCATCCACTGGTGCCTCCCCGGGCTGCCGGACACATGGAATGAGTTCCTTTACACGCGTATCATCTCGCGGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010383 GO:0010410 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0070589 GO:0070592 GO:0071554 GO:0071704 GO:0071840 GO:0080090 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990538 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

503

Amino Acids

58.51

Weight (kDa)

5.38

Isoelectric Point (pI)

49.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	154 - 207	1.2e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	208 - 496	4.2e-93	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004037)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40150 AT2G40160 AT2G40160 AT2G40160 AT3G55990
fragaria_vesca	FvH4_7g24620
malus_domestica	MD01G1154500.v1.1 MD07G1223300.v1.1
prunus_persica	Prupe.2G251900_v2.0.a1
pyrus_communis	pycom01g17170 pycom07g20190 pycom07g20200
rosa_chinensis	RchiOBHm_Chr1g0367551 RchiOBHm_Chr1g0370451
rosa_laevigata	RLG00000004529 RLG00000004557 RLG00000008944 RLG00000027040 RLG00000036183
rosa_multiflora	Rmu_sc0005656.1_g000014
rosa_roxburghii	Rroxscaffold_4G00286550
rosa_rugosa	Rorug01G0356900
rosa_samantha	Rh1BG328300 Rh1CG341400 Rh2CG472100 Rh2CG472200 Rh2DG621700 Rh7DG249300 Rh7DG249400 Rh7DG445600
rosa_wichuraiana	Rw1G010640 Rw1G032200 Rw6G015350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	144
AccB1I	GGYRCC	3 cut(s)	832, 1022, 1443
AccII	CGCG	3 cut(s)	155, 1489, 1501
AciI	CCGC	3 cut(s)	64, 155, 1501
AclWI	GGATC	4 cut(s)	488, 784, 857, 1015
AcoI	YGGCCR	1 cut(s)	1049
AcsI	RAATTY	3 cut(s)	158, 853, 996
AcuI	CTGAAG	3 cut(s)	63, 360, 477
AcyI	GRCGYC	3 cut(s)	141, 1023, 1394
AfaI	GTAC	1 cut(s)	131
AfiI	CCNNNNNNNGG	2 cut(s)	274, 1054
AflIII	ACRYGT	2 cut(s)	981, 1487
AgsI	TTSAA	6 cut(s)	446, 634, 697, 958, 988, 1275
AhdI	GACNNNNNGTC	1 cut(s)	145
AjnI	CCWGG	1 cut(s)	748
AluBI	AGCT	6 cut(s)	47, 222, 364, 762, 1164, 1409
AluI	AGCT	6 cut(s)	47, 222, 364, 762, 1164, 1409
Alw21I	GWGCWC	1 cut(s)	224
Alw26I	GTCTC	4 cut(s)	338, 1149, 1199, 1412
AlwI	GGATC	4 cut(s)	488, 784, 857, 1015
AlwNI	CAGNNNCTG	2 cut(s)	233, 338
Ama87I	CYCGRG	1 cut(s)	1451
AoxI	GGCC	1 cut(s)	1049
ApeKI	GCWGC	3 cut(s)	604, 1402, 1456
ApoI	RAATTY	3 cut(s)	158, 853, 996
ArsI	GACNNNNNNTTYG	2 cut(s)	493, 525
Asp700I	GAANNNNTTC	2 cut(s)	983, 1475
AspLEI	GCGC	3 cut(s)	187, 290, 1025
AspS9I	GGNCC	1 cut(s)	1119
AsuC2I	CCSGG	4 cut(s)	1181, 1415, 1452, 1453
AsuHPI	GGTGA	3 cut(s)	427, 778, 1126
AsuII	TTCGAA	1 cut(s)	239
AvaI	CYCGRG	1 cut(s)	1451
AvaII	GGWCC	1 cut(s)	1119
BaeGI	GKGCMC	2 cut(s)	835, 1250
BaeI	ACNNNNGTAYC	2 cut(s)	1474, 1507
BanI	GGYRCC	3 cut(s)	832, 1022, 1443
BanII	GRGCYC	1 cut(s)	224
BbsI	GAAGAC	5 cut(s)	91, 144, 399, 528, 1120
Bbv12I	GWGCWC	1 cut(s)	224
BbvI	GCAGC	3 cut(s)	616, 1414, 1443
BccI	CCATC	5 cut(s)	158, 370, 594, 1178, 1379
BceAI	ACGGC	2 cut(s)	558, 1036
BcgI	CGANNNNNNTGC	1 cut(s)	27
BciT130I	CCWGG	1 cut(s)	750
BclI	TGATCA	1 cut(s)	1015
BcnI	CCSGG	4 cut(s)	1181, 1415, 1452, 1453
BcoDI	GTCTC	4 cut(s)	338, 1149, 1199, 1412
BfaI	CTAG	2 cut(s)	650, 950
BfmI	CTRYAG	1 cut(s)	813
BfoI	RGCGCY	1 cut(s)	1026
BisI	GCNGC	4 cut(s)	64, 605, 1403, 1457
BlsI	GCNGC	4 cut(s)	65, 606, 1404, 1458
Bme1390I	CCNGG	5 cut(s)	750, 1181, 1415, 1452, 1453
Bme18I	GGWCC	1 cut(s)	1119
BmeRI	GACNNNNNGTC	1 cut(s)	145
BmeT110I	CYCGRG	1 cut(s)	1451
BmgT120I	GGNCC	1 cut(s)	1119
BmiI	GGNNCC	3 cut(s)	834, 1024, 1445
BmrFI	CCNGG	5 cut(s)	750, 1181, 1415, 1452, 1453
BmrI	ACTGGG	2 cut(s)	313, 838
BmsI	GCATC	3 cut(s)	861, 1339, 1443
BmuI	ACTGGG	2 cut(s)	313, 838
BoxI	GACNNNNGTC	1 cut(s)	1124
BpiI	GAAGAC	5 cut(s)	91, 144, 399, 528, 1120
BpmI	CTGGAG	1 cut(s)	1197
Bpu14I	TTCGAA	1 cut(s)	239
BpuEI	CTTGAG	1 cut(s)	531
BpuMI	CCSGG	4 cut(s)	1181, 1415, 1452, 1453
BsaBI	GATNNNNATC	1 cut(s)	618
BsaHI	GRCGYC	3 cut(s)	141, 1023, 1394
BsaI	GGTCTC	3 cut(s)	338, 1199, 1412
BsaJI	CCNNGG	4 cut(s)	748, 749, 1450, 1451
BsaWI	WCCGGW	1 cut(s)	1062
Bsc4I	CCNNNNNNNGG	2 cut(s)	274, 1054
Bse118I	RCCGGY	1 cut(s)	1047
Bse1I	ACTGG	6 cut(s)	319, 332, 706, 833, 1214, 1445
Bse3DI	GCAATG	1 cut(s)	1299
Bse8I	GATNNNNATC	1 cut(s)	618
BseBI	CCWGG	1 cut(s)	750
BseDI	CCNNGG	4 cut(s)	748, 749, 1450, 1451
BseGI	GGATG	5 cut(s)	505, 943, 981, 1189, 1434
BseJI	GATNNNNATC	1 cut(s)	618
BseLI	CCNNNNNNNGG	2 cut(s)	274, 1054
BseMI	GCAATG	1 cut(s)	1299
BseMII	CTCAG	5 cut(s)	240, 650, 746, 1326, 1374
BseNI	ACTGG	6 cut(s)	319, 332, 706, 833, 1214, 1445
BseRI	GAGGAG	2 cut(s)	214, 1137
BseSI	GKGCMC	2 cut(s)	835, 1250
BseX3I	CGGCCG	1 cut(s)	1049
BseXI	GCAGC	3 cut(s)	616, 1414, 1443
Bsh1236I	CGCG	3 cut(s)	155, 1489, 1501
Bsh1285I	CGRYCG	1 cut(s)	1052
BshFI	GGCC	1 cut(s)	1051
BshNI	GGYRCC	3 cut(s)	832, 1022, 1443
BsiEI	CGRYCG	1 cut(s)	1052
BsiHKAI	GWGCWC	1 cut(s)	224
BsiHKCI	CYCGRG	1 cut(s)	1451
BsiSI	CCGG	7 cut(s)	1048, 1063, 1181, 1397, 1415, 1452, 1460
BslFI	GGGAC	1 cut(s)	1187
BslI	CCNNNNNNNGG	2 cut(s)	274, 1054
BsmAI	GTCTC	4 cut(s)	338, 1149, 1199, 1412
BsmFI	GGGAC	1 cut(s)	1187
BsnI	GGCC	1 cut(s)	1051
Bso31I	GGTCTC	3 cut(s)	338, 1199, 1412
BsoBI	CYCGRG	1 cut(s)	1451
Bsp119I	TTCGAA	1 cut(s)	239
Bsp1286I	GDGCHC	3 cut(s)	224, 835, 1250
Bsp1407I	TGTACA	1 cut(s)	129
Bsp143I	GATC	6 cut(s)	493, 776, 862, 909, 1007, 1015
BspACI	CCGC	3 cut(s)	64, 155, 1501
BspANI	GGCC	1 cut(s)	1051
BspCNI	CTCAG	5 cut(s)	239, 651, 745, 1327, 1373
BspFNI	CGCG	3 cut(s)	155, 1489, 1501
BspLI	GGNNCC	3 cut(s)	834, 1024, 1445
BspPI	GGATC	4 cut(s)	488, 784, 857, 1015
BspQI	GCTCTTC	1 cut(s)	53
BspT104I	TTCGAA	1 cut(s)	239
BspT107I	GGYRCC	3 cut(s)	832, 1022, 1443
BspTNI	GGTCTC	3 cut(s)	338, 1199, 1412
BsrDI	GCAATG	1 cut(s)	1299
BsrFI	RCCGGY	1 cut(s)	1047
BsrGI	TGTACA	1 cut(s)	129
BsrI	ACTGG	6 cut(s)	319, 332, 706, 833, 1214, 1445
BssAI	RCCGGY	1 cut(s)	1047
BssECI	CCNNGG	4 cut(s)	748, 749, 1450, 1451
BssMI	GATC	6 cut(s)	493, 776, 862, 909, 1007, 1015
BssNI	GRCGYC	3 cut(s)	141, 1023, 1394
Bst2UI	CCWGG	1 cut(s)	750
Bst4CI	ACNGT	4 cut(s)	817, 890, 1126, 1236
Bst6I	CTCTTC	6 cut(s)	53, 175, 336, 375, 432, 1155
BstACI	GRCGYC	3 cut(s)	141, 1023, 1394
BstAUI	TGTACA	1 cut(s)	129
BstBI	TTCGAA	1 cut(s)	239
BstC8I	GCNNGC	1 cut(s)	1407
BstDEI	CTNAG	6 cut(s)	226, 284, 659, 732, 1335, 1360
BstF5I	GGATG	5 cut(s)	505, 943, 981, 1189, 1434
BstFNI	CGCG	3 cut(s)	155, 1489, 1501
BstH2I	RGCGCY	1 cut(s)	1026
BstHHI	GCGC	3 cut(s)	187, 290, 1025
BstKTI	GATC	6 cut(s)	496, 779, 865, 912, 1010, 1018
BstMAI	GTCTC	4 cut(s)	338, 1149, 1199, 1412
BstMBI	GATC	6 cut(s)	493, 776, 862, 909, 1007, 1015
BstMCI	CGRYCG	1 cut(s)	1052
BstMWI	GCNNNNNNNGC	1 cut(s)	1402
BstNI	CCWGG	1 cut(s)	750
BstNSI	RCATGY	1 cut(s)	1144
BstPAI	GACNNNNGTC	1 cut(s)	1124
BstSCI	CCNGG	5 cut(s)	748, 1179, 1413, 1450, 1451
BstSFI	CTRYAG	1 cut(s)	813
BstSLI	GKGCMC	2 cut(s)	835, 1250
BstUI	CGCG	3 cut(s)	155, 1489, 1501
BstV1I	GCAGC	3 cut(s)	616, 1414, 1443
BstV2I	GAAGAC	5 cut(s)	91, 144, 399, 528, 1120
BstX2I	RGATCY	1 cut(s)	776
BstYI	RGATCY	1 cut(s)	776
BstZI	CGGCCG	1 cut(s)	1049
BsuRI	GGCC	1 cut(s)	1051
BtgZI	GCGATG	1 cut(s)	136
BtsCI	GGATG	5 cut(s)	505, 943, 981, 1189, 1434
BtsIMutI	CAGTG	4 cut(s)	713, 822, 1122, 1438
Cac8I	GCNNGC	1 cut(s)	1407
CaiI	CAGNNNCTG	2 cut(s)	233, 338
CfoI	GCGC	3 cut(s)	187, 290, 1025
Cfr10I	RCCGGY	1 cut(s)	1047
Cfr13I	GGNCC	1 cut(s)	1119
Cfr9I	CCCGGG	1 cut(s)	1451
CseI	GACGC	1 cut(s)	1402
Csp6I	GTAC	1 cut(s)	130
CviAII	CATG	8 cut(s)	127, 676, 718, 920, 991, 1080, 1141, 1467
CviQI	GTAC	1 cut(s)	130
DdeI	CTNAG	6 cut(s)	226, 284, 659, 732, 1335, 1360
DinI	GGCGCC	1 cut(s)	1024
DpnI	GATC	6 cut(s)	495, 778, 864, 911, 1009, 1017
DpnII	GATC	6 cut(s)	493, 776, 862, 909, 1007, 1015
DriI	GACNNNNNGTC	1 cut(s)	145
EaeI	YGGCCR	1 cut(s)	1049
EagI	CGGCCG	1 cut(s)	1049
Eam1104I	CTCTTC	6 cut(s)	53, 175, 336, 375, 432, 1155
Eam1105I	GACNNNNNGTC	1 cut(s)	145
EarI	CTCTTC	6 cut(s)	53, 175, 336, 375, 432, 1155
Ecl136II	GAGCTC	1 cut(s)	222
EclXI	CGGCCG	1 cut(s)	1049
Eco24I	GRGCYC	1 cut(s)	224
Eco31I	GGTCTC	3 cut(s)	338, 1199, 1412
Eco47I	GGWCC	1 cut(s)	1119
Eco52I	CGGCCG	1 cut(s)	1049
Eco53kI	GAGCTC	1 cut(s)	222
Eco57I	CTGAAG	3 cut(s)	63, 360, 477
Eco88I	CYCGRG	1 cut(s)	1451
EcoICRI	GAGCTC	1 cut(s)	222
EcoRI	GAATTC	1 cut(s)	158
EcoRII	CCWGG	1 cut(s)	748
EcoT38I	GRGCYC	1 cut(s)	224
EgeI	GGCGCC	1 cut(s)	1024
EheI	GGCGCC	1 cut(s)	1024
FaeI	CATG	8 cut(s)	130, 679, 721, 923, 994, 1083, 1144, 1470
FaqI	GGGAC	1 cut(s)	1187
FatI	CATG	8 cut(s)	126, 675, 717, 919, 990, 1079, 1140, 1466
FbaI	TGATCA	1 cut(s)	1015
Fnu4HI	GCNGC	4 cut(s)	64, 605, 1403, 1457
FokI	GGATG	5 cut(s)	492, 950, 988, 1196, 1421
FriOI	GRGCYC	1 cut(s)	224
Fsp4HI	GCNGC	4 cut(s)	64, 605, 1403, 1457
FspBI	CTAG	2 cut(s)	650, 950
GlaI	GCGC	3 cut(s)	186, 289, 1024
GluI	GCNGC	4 cut(s)	64, 605, 1403, 1457
GsuI	CTGGAG	1 cut(s)	1197
HaeII	RGCGCY	1 cut(s)	1026
HaeIII	GGCC	1 cut(s)	1051
HapII	CCGG	7 cut(s)	1048, 1063, 1181, 1397, 1415, 1452, 1460
HgaI	GACGC	1 cut(s)	1402
HhaI	GCGC	3 cut(s)	187, 290, 1025
Hin1I	GRCGYC	3 cut(s)	141, 1023, 1394
Hin1II	CATG	8 cut(s)	130, 679, 721, 923, 994, 1083, 1144, 1470
Hin6I	GCGC	3 cut(s)	185, 288, 1023
HinP1I	GCGC	3 cut(s)	185, 288, 1023
HincII	GTYRAC	1 cut(s)	1392
HindII	GTYRAC	1 cut(s)	1392
HindIII	AAGCTT	1 cut(s)	45
HinfI	GANTC	8 cut(s)	230, 251, 328, 502, 578, 689, 713, 848
HpaII	CCGG	7 cut(s)	1048, 1063, 1181, 1397, 1415, 1452, 1460
HphI	GGTGA	3 cut(s)	427, 778, 1126
Hpy166II	GTNNAC	3 cut(s)	1096, 1309, 1392
Hpy188I	TCNGA	6 cut(s)	229, 262, 340, 859, 1119, 1336
Hpy188III	TCNNGA	8 cut(s)	118, 268, 455, 538, 913, 1159, 1219, 1506
Hpy8I	GTNNAC	3 cut(s)	1096, 1309, 1392
Hpy99I	CGWCG	2 cut(s)	95, 98
HpyAV	CCTTC	3 cut(s)	37, 928, 1251
HpyCH4III	ACNGT	4 cut(s)	817, 890, 1126, 1236
HpyCH4IV	ACGT	2 cut(s)	141, 983
HpyCH4V	TGCA	7 cut(s)	4, 205, 296, 638, 874, 1352, 1434
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1402
HpyF3I	CTNAG	6 cut(s)	226, 284, 659, 732, 1335, 1360
HpySE526I	ACGT	2 cut(s)	141, 983
Hsp92I	GRCGYC	3 cut(s)	141, 1023, 1394
Hsp92II	CATG	8 cut(s)	130, 679, 721, 923, 994, 1083, 1144, 1470
HspAI	GCGC	3 cut(s)	185, 288, 1023
KasI	GGCGCC	1 cut(s)	1022
Ksp22I	TGATCA	1 cut(s)	1015
Kzo9I	GATC	6 cut(s)	493, 776, 862, 909, 1007, 1015
LguI	GCTCTTC	1 cut(s)	53
LmnI	GCTCC	3 cut(s)	227, 361, 1399
Lsp1109I	GCAGC	3 cut(s)	616, 1414, 1443
LweI	GCATC	3 cut(s)	861, 1339, 1443
MaeI	CTAG	2 cut(s)	650, 950
MaeII	ACGT	2 cut(s)	141, 983
MaeIII	GTNAC	3 cut(s)	464, 499, 550
MalI	GATC	6 cut(s)	495, 778, 864, 911, 1009, 1017
MboI	GATC	6 cut(s)	493, 776, 862, 909, 1007, 1015
MfeI	CAATTG	1 cut(s)	305
MflI	RGATCY	1 cut(s)	776
MhlI	GDGCHC	3 cut(s)	224, 835, 1250
MluCI	AATT	8 cut(s)	158, 305, 446, 741, 795, 853, 928, 996
MluI	ACGCGT	1 cut(s)	1487
Mly113I	GGCGCC	1 cut(s)	1023
MlyI	GAGTC	5 cut(s)	224, 245, 496, 572, 857
MmeI	TCCRAC	2 cut(s)	303, 1218
MroXI	GAANNNNTTC	2 cut(s)	983, 1475
MseI	TTAA	2 cut(s)	474, 798
MslI	CAYNNNNRTG	2 cut(s)	680, 1139
MspI	CCGG	7 cut(s)	1048, 1063, 1181, 1397, 1415, 1452, 1460
MspR9I	CCNGG	5 cut(s)	750, 1181, 1415, 1452, 1453
MunI	CAATTG	1 cut(s)	305
MvaI	CCWGG	1 cut(s)	750
MvnI	CGCG	3 cut(s)	155, 1489, 1501
MwoI	GCNNNNNNNGC	1 cut(s)	1402
NarI	GGCGCC	1 cut(s)	1023
NciI	CCSGG	4 cut(s)	1181, 1415, 1452, 1453
NdeII	GATC	6 cut(s)	493, 776, 862, 909, 1007, 1015
NlaIII	CATG	8 cut(s)	130, 679, 721, 923, 994, 1083, 1144, 1470
NlaIV	GGNNCC	3 cut(s)	834, 1024, 1445
NmuCI	GTSAC	2 cut(s)	464, 499
NspI	RCATGY	1 cut(s)	1144
NspV	TTCGAA	1 cut(s)	239
PasI	CCCWGGG	1 cut(s)	749
PciSI	GCTCTTC	1 cut(s)	53
PdmI	GAANNNNTTC	2 cut(s)	983, 1475
PfeI	GAWTC	3 cut(s)	328, 689, 713
PkrI	GCNGC	4 cut(s)	65, 606, 1404, 1458
PleI	GAGTC	5 cut(s)	224, 245, 496, 572, 856
PluTI	GGCGCC	1 cut(s)	1026
PpsI	GAGTC	5 cut(s)	224, 245, 496, 572, 856
PshAI	GACNNNNGTC	1 cut(s)	1124
Psp124BI	GAGCTC	1 cut(s)	224
Psp6I	CCWGG	1 cut(s)	748
PspGI	CCWGG	1 cut(s)	748
PspN4I	GGNNCC	3 cut(s)	834, 1024, 1445
PspPI	GGNCC	1 cut(s)	1119
PstNI	CAGNNNCTG	2 cut(s)	233, 338
PsuI	RGATCY	1 cut(s)	776
RsaI	GTAC	1 cut(s)	131
RsaNI	GTAC	1 cut(s)	130
RseI	CAYNNNNRTG	2 cut(s)	680, 1139
SacI	GAGCTC	1 cut(s)	224
SapI	GCTCTTC	1 cut(s)	53
SaqAI	TTAA	2 cut(s)	474, 798
SatI	GCNGC	4 cut(s)	64, 605, 1403, 1457
Sau3AI	GATC	6 cut(s)	493, 776, 862, 909, 1007, 1015
Sau96I	GGNCC	1 cut(s)	1119
SchI	GAGTC	5 cut(s)	224, 245, 496, 572, 857
ScrFI	CCNGG	5 cut(s)	750, 1181, 1415, 1452, 1453
SduI	GDGCHC	3 cut(s)	224, 835, 1250
SfaNI	GCATC	3 cut(s)	861, 1339, 1443
SfcI	CTRYAG	1 cut(s)	813
SfoI	GGCGCC	1 cut(s)	1024
SfuI	TTCGAA	1 cut(s)	239
SinI	GGWCC	1 cut(s)	1119
SmaI	CCCGGG	1 cut(s)	1453
SmiMI	CAYNNNNRTG	2 cut(s)	680, 1139
SmlI	CTYRAG	1 cut(s)	546
SmoI	CTYRAG	1 cut(s)	546
Sse9I	AATT	8 cut(s)	158, 305, 446, 741, 795, 853, 928, 996
SsiI	CCGC	3 cut(s)	64, 155, 1501
SspDI	GGCGCC	1 cut(s)	1022
SspI	AATATT	1 cut(s)	1105
SspMI	CTAG	2 cut(s)	650, 950
SstI	GAGCTC	1 cut(s)	224
StyD4I	CCNGG	5 cut(s)	748, 1179, 1413, 1450, 1451
TaaI	ACNGT	4 cut(s)	817, 890, 1126, 1236
TaiI	ACGT	2 cut(s)	144, 986
TaqI	TCGA	3 cut(s)	239, 492, 908
TasI	AATT	8 cut(s)	158, 305, 446, 741, 795, 853, 928, 996
TatI	WGTACW	1 cut(s)	129
TauI	GCSGC	1 cut(s)	66
TfiI	GAWTC	3 cut(s)	328, 689, 713
Tru1I	TTAA	2 cut(s)	474, 798
Tru9I	TTAA	2 cut(s)	474, 798
TscAI	CASTG	4 cut(s)	713, 822, 1129, 1445
TseFI	GTSAC	2 cut(s)	464, 499
TseI	GCWGC	3 cut(s)	604, 1402, 1456
Tsp45I	GTSAC	2 cut(s)	464, 499
TspDTI	ATGAA	7 cut(s)	71, 115, 150, 435, 992, 1007, 1310
TspGWI	ACGGA	1 cut(s)	449
TspMI	CCCGGG	1 cut(s)	1451
TspRI	CASTG	4 cut(s)	713, 822, 1129, 1445
VpaK11BI	GGWCC	1 cut(s)	1119
XapI	RAATTY	3 cut(s)	158, 853, 996
XceI	RCATGY	1 cut(s)	1144
XmaI	CCCGGG	1 cut(s)	1451
XmnI	GAANNNNTTC	2 cut(s)	983, 1475
XspI	CTAG	2 cut(s)	650, 950
ZraI	GACGTC	1 cut(s)	142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.