Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004037

Physical Location & Seq

Genomic Coordinates Forward (+)

30019894 .. 30023845

Gene Structure 3952 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1223300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGCAGCTACCTCGCAGAAAAACTCTCTCTCTCTCCGAATCTGCGGCAATGAAGGTACGCAAGAACAATAACCTCTCCATCTTCGTCGTCGTCTTTTCGGTCTTCCTCTTCGGGGTGTTCATGTATAATGAGGACGTGAAATCCATCGCCGAGTTTCCATTCTCGAGCCACAAAGCTCAAGAACTCCAGGAAGAACTCCAGTTGTCGAGACAGAGCGACCCGGTTCAAGAAACGAAACAGACCGACTCTGTTCAAGAAACCCACAACAGAGTGTCTCTGAGCTCGAGAACCCAACTGGAAGAAACCCATTTGGAGAATCACAATTCAGAGGAAACCCAGTTGGAAAATCACAACTCAGAGGAAACCCAGTTGGAGGATCACAGTTCAGAAGAAACCCAGTTGGAGAACAACAATTCGGATGAAACCCTAGAGCCGGTTGATCTGAAATCCATCGTCGAACAACACAAAGATCAGAAGATTGAATTGTTGGGTTCTTCTCAGGAAGAAGAGGAAGAGGAGGAGATTGAACTGCCGCCGGAAGATTGCGATTTGTTCAACGGAGATTGGGTTTTCGATCCCGCGACTCACCCCTTATACAAAGAAGAAGAATGCGAGTTTCTTACGGAGCAAGTGACGTGCATGAGAAATGGAAGGAAGGACTCTCTGTACCAGCATTGGAGATGGCAGCCCAGAGACTGTAATCTCCCGAAGTTTAATGCGAGATTGTTGCTAGAGAAATTGAGGAACAAGAGGCTGATGTTTGTTGGAGATTCACTGAACAGAAACCAGTGGGAATCCATGATTTGTTTTGCTCAGTCTGCCGTTCCTCCCGGCAGGAAGAGCCTGGTGAAGAATGGCTCCCTCTCTGTTTTCAGAGTTGAGGACTATAATGCCACGGTGGAATTTTATTGGGCACCATTTCTGGTGGAGTCAAATTCAGATGATCCAAACATGCACAGCATATTAAATCGAATAATAATGCCCAAATCAATTAAAAAGCATGGTGAAAATTGGAAGGGCGTCGACTATCTAATTTTCAACACATACATTTGGTGGATGAACACTTTCAACATGAAAGTTCTACGAGGTTCGTTCGATCAAGGGGCAACGGAATACGATGAGATCGACCGGCCGTGGGCGTACCGGAGAGTCATGAAAACTTGGGCTAAGTGGGTGGACAAGAACATTGATCCTAATCGGACCACCGTTTTCTTCAGCAGCATGTCTCCTCTTCACATCAAGAGTTTGGATTGGGACAACCCAAATGGCATAAAATGCGCAAAAGAAACCACACCAGTTTTGAACATGACAACCCCATTGGAAGTGGGCACGGACCGCAGGCTATTTGTGATTTCAAGCAACATAACAAAATCAATGAAAGTGCCCGTCTACTTTCTCAACATCACAACCCTCTCCGAGTTCCGCAAAGATGCACATACCTCAGTCTACACCATCAGGCAAGGCAAGTTGTTGACGCCGGAGCAGCAAGCCGACCCGGCCACTTACGCAGACTGCATCCATTGGTGCCTCCCCGGGTTGCCCGACACATGGAACGAGTTTCTTTACTCACGTATCATCTCCCGCTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010383 GO:0010410 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0070589 GO:0070592 GO:0071554 GO:0071704 GO:0071840 GO:0080090 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990538 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

530

Amino Acids

61.78

Weight (kDa)

5.19

Isoelectric Point (pI)

57.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	181 - 234	2.3e-23	PMR5 N terminal Domain
PC-Esterase	PF13839	235 - 523	1.1e-94	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004037)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40150 AT2G40160 AT2G40160 AT2G40160 AT3G55990
fragaria_vesca	FvH4_7g24620
malus_domestica	MD01G1154500.v1.1 MD07G1223300.v1.1
prunus_persica	Prupe.2G251900_v2.0.a1
pyrus_communis	pycom01g17170 pycom07g20190 pycom07g20200
rosa_chinensis	RchiOBHm_Chr1g0367551 RchiOBHm_Chr1g0370451
rosa_laevigata	RLG00000004529 RLG00000004557 RLG00000008944 RLG00000027040 RLG00000036183
rosa_multiflora	Rmu_sc0005656.1_g000014
rosa_roxburghii	Rroxscaffold_4G00286550
rosa_rugosa	Rorug01G0356900
rosa_samantha	Rh1BG328300 Rh1CG341400 Rh2CG472100 Rh2CG472200 Rh2DG621700 Rh7DG249300 Rh7DG249400 Rh7DG445600
rosa_wichuraiana	Rw1G010640 Rw1G032200 Rw6G015350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1279
AccB1I	GGYRCC	2 cut(s)	913, 1524
AccBSI	CCGCTC	1 cut(s)	1584
AccI	GTMKAC	3 cut(s)	1023, 1389, 1446
AccII	CGCG	1 cut(s)	581
AciI	CCGC	6 cut(s)	44, 533, 579, 1336, 1423, 1582
AclWI	GGATC	4 cut(s)	384, 569, 938, 1184
AcoI	YGGCCR	2 cut(s)	1130, 1497
AcsI	RAATTY	2 cut(s)	902, 934
AcuI	CTGAAG	1 cut(s)	1198
AcyI	GRCGYC	2 cut(s)	1020, 1475
AfaI	GTAC	3 cut(s)	57, 668, 1142
AfiI	CCNNNNNNNGG	5 cut(s)	112, 373, 433, 1135, 1548
AgsI	TTSAA	9 cut(s)	227, 254, 482, 527, 556, 1039, 1069, 1303, 1356
AjiI	CACGTC	2 cut(s)	136, 636
AjnI	CCWGG	2 cut(s)	186, 843
AluBI	AGCT	3 cut(s)	7, 176, 282
AluI	AGCT	3 cut(s)	7, 176, 282
Alw21I	GWGCWC	1 cut(s)	284
Alw26I	GTCTC	4 cut(s)	202, 279, 687, 1230
AlwI	GGATC	4 cut(s)	384, 569, 938, 1184
AlwNI	CAGNNNCTG	1 cut(s)	696
Ama87I	CYCGRG	3 cut(s)	163, 283, 1532
AoxI	GGCC	2 cut(s)	1130, 1497
ApeKI	GCWGC	4 cut(s)	4, 685, 1218, 1483
ApoI	RAATTY	2 cut(s)	902, 934
Asp700I	GAANNNNTTC	1 cut(s)	1064
AspLEI	GCGC	1 cut(s)	1280
AspS9I	GGNCC	2 cut(s)	1200, 1333
AsuC2I	CCSGG	5 cut(s)	221, 831, 1496, 1533, 1534
AsuHPI	GGTGA	3 cut(s)	578, 859, 1016
AvaI	CYCGRG	3 cut(s)	163, 283, 1532
AvaII	GGWCC	2 cut(s)	1200, 1333
BaeGI	GKGCMC	3 cut(s)	916, 1331, 1386
BaeI	ACNNNNGTAYC	2 cut(s)	1555, 1588
BanI	GGYRCC	2 cut(s)	913, 1524
BanII	GRGCYC	1 cut(s)	284
BbsI	GAAGAC	1 cut(s)	94
Bbv12I	GWGCWC	1 cut(s)	284
BbvI	GCAGC	4 cut(s)	16, 697, 1230, 1495
BccI	CCATC	5 cut(s)	86, 152, 458, 675, 1460
BceAI	ACGGC	2 cut(s)	806, 1117
BcgI	CGANNNNNNTGC	3 cut(s)	27, 709, 743
BciT130I	CCWGG	2 cut(s)	188, 845
BcnI	CCSGG	5 cut(s)	221, 831, 1496, 1533, 1534
BcoDI	GTCTC	4 cut(s)	202, 279, 687, 1230
BfaI	CTAG	2 cut(s)	428, 731
BglI	GCCNNNNNGGC	1 cut(s)	1496
BisI	GCNGC	6 cut(s)	5, 45, 533, 686, 1219, 1484
BlsI	GCNGC	6 cut(s)	6, 46, 534, 687, 1220, 1485
Bme1390I	CCNGG	7 cut(s)	188, 221, 831, 845, 1496, 1533, 1534
Bme18I	GGWCC	2 cut(s)	1200, 1333
BmeT110I	CYCGRG	3 cut(s)	163, 283, 1532
BmgBI	CACGTC	2 cut(s)	136, 636
BmgT120I	GGNCC	2 cut(s)	1200, 1333
BmiI	GGNNCC	3 cut(s)	859, 915, 1526
BmrFI	CCNGG	7 cut(s)	188, 221, 831, 845, 1496, 1533, 1534
BmrI	ACTGGG	3 cut(s)	331, 361, 391
BmsI	GCATC	2 cut(s)	1420, 1524
BmuI	ACTGGG	3 cut(s)	331, 361, 391
BpiI	GAAGAC	1 cut(s)	94
BpmI	CTGGAG	2 cut(s)	170, 182
BpuEI	CTTGAG	1 cut(s)	162
BpuMI	CCSGG	5 cut(s)	221, 831, 1496, 1533, 1534
BsaAI	YACGTR	1 cut(s)	1571
BsaBI	GATNNNNATC	1 cut(s)	1194
BsaHI	GRCGYC	2 cut(s)	1020, 1475
BsaJI	CCNNGG	4 cut(s)	894, 1133, 1531, 1532
BsaWI	WCCGGW	1 cut(s)	1143
BsaXI	ACNNNNNCTCC	2 cut(s)	552, 582
Bsc4I	CCNNNNNNNGG	5 cut(s)	112, 373, 433, 1135, 1548
Bse118I	RCCGGY	2 cut(s)	433, 1128
Bse1I	ACTGG	7 cut(s)	199, 300, 337, 367, 397, 787, 1295
Bse3DI	GCAATG	1 cut(s)	54
Bse8I	GATNNNNATC	1 cut(s)	1194
BseBI	CCWGG	2 cut(s)	188, 845
BseDI	CCNNGG	4 cut(s)	894, 1133, 1531, 1532
BseGI	GGATG	3 cut(s)	424, 1062, 1515
BseJI	GATNNNNATC	1 cut(s)	1194
BseLI	CCNNNNNNNGG	5 cut(s)	112, 373, 433, 1135, 1548
BseMI	GCAATG	1 cut(s)	54
BseMII	CTCAG	5 cut(s)	269, 369, 512, 827, 1455
BseNI	ACTGG	7 cut(s)	199, 300, 337, 367, 397, 787, 1295
BseRI	GAGGAG	3 cut(s)	530, 533, 1218
BseSI	GKGCMC	3 cut(s)	916, 1331, 1386
BseX3I	CGGCCG	1 cut(s)	1130
BseXI	GCAGC	4 cut(s)	16, 697, 1230, 1495
Bsh1236I	CGCG	1 cut(s)	581
Bsh1285I	CGRYCG	2 cut(s)	1129, 1133
BshFI	GGCC	2 cut(s)	1132, 1499
BshNI	GGYRCC	2 cut(s)	913, 1524
BsiEI	CGRYCG	2 cut(s)	1129, 1133
BsiHKAI	GWGCWC	1 cut(s)	284
BsiHKCI	CYCGRG	3 cut(s)	163, 283, 1532
BsiSI	CCGG	9 cut(s)	221, 434, 536, 831, 1129, 1144, 1478, 1496, 1533
BslFI	GGGAC	1 cut(s)	1268
BslI	CCNNNNNNNGG	5 cut(s)	112, 373, 433, 1135, 1548
BsmAI	GTCTC	4 cut(s)	202, 279, 687, 1230
BsmFI	GGGAC	1 cut(s)	1268
BsmI	GAATGC	1 cut(s)	614
BsnI	GGCC	2 cut(s)	1132, 1499
BsoBI	CYCGRG	3 cut(s)	163, 283, 1532
Bsp1286I	GDGCHC	4 cut(s)	284, 916, 1331, 1386
Bsp143I	GATC	8 cut(s)	376, 439, 469, 574, 943, 1096, 1122, 1189
BspACI	CCGC	6 cut(s)	44, 533, 579, 1336, 1423, 1582
BspANI	GGCC	2 cut(s)	1132, 1499
BspCNI	CTCAG	5 cut(s)	270, 368, 511, 826, 1454
BspFNI	CGCG	1 cut(s)	581
BspHI	TCATGA	1 cut(s)	1152
BspLI	GGNNCC	3 cut(s)	859, 915, 1526
BspPI	GGATC	4 cut(s)	384, 569, 938, 1184
BspQI	GCTCTTC	1 cut(s)	833
BspT107I	GGYRCC	2 cut(s)	913, 1524
BsrBI	CCGCTC	1 cut(s)	1584
BsrDI	GCAATG	1 cut(s)	54
BsrFI	RCCGGY	2 cut(s)	433, 1128
BsrI	ACTGG	7 cut(s)	199, 300, 337, 367, 397, 787, 1295
BssAI	RCCGGY	2 cut(s)	433, 1128
BssECI	CCNNGG	4 cut(s)	894, 1133, 1531, 1532
BssMI	GATC	8 cut(s)	376, 439, 469, 574, 943, 1096, 1122, 1189
BssNI	GRCGYC	2 cut(s)	1020, 1475
Bst2UI	CCWGG	2 cut(s)	188, 845
Bst4CI	ACNGT	4 cut(s)	383, 698, 898, 1207
Bst6I	CTCTTC	5 cut(s)	113, 501, 507, 833, 1236
BstACI	GRCGYC	2 cut(s)	1020, 1475
BstAPI	GCANNNNNTGC	1 cut(s)	1275
BstBAI	YACGTR	1 cut(s)	1571
BstC8I	GCNNGC	2 cut(s)	1340, 1488
BstDEI	CTNAG	6 cut(s)	278, 355, 498, 813, 1167, 1441
BstDSI	CCRYGG	2 cut(s)	894, 1133
BstF5I	GGATG	3 cut(s)	424, 1062, 1515
BstFNI	CGCG	1 cut(s)	581
BstHHI	GCGC	1 cut(s)	1280
BstKTI	GATC	8 cut(s)	379, 442, 472, 577, 946, 1099, 1125, 1192
BstMAI	GTCTC	4 cut(s)	202, 279, 687, 1230
BstMBI	GATC	8 cut(s)	376, 439, 469, 574, 943, 1096, 1122, 1189
BstMCI	CGRYCG	2 cut(s)	1129, 1133
BstMWI	GCNNNNNNNGC	7 cut(s)	818, 840, 1275, 1335, 1483, 1496, 1505
BstNI	CCWGG	2 cut(s)	188, 845
BstNSI	RCATGY	2 cut(s)	955, 1225
BstSCI	CCNGG	7 cut(s)	186, 219, 829, 843, 1494, 1531, 1532
BstSLI	GKGCMC	3 cut(s)	916, 1331, 1386
BstUI	CGCG	1 cut(s)	581
BstV1I	GCAGC	4 cut(s)	16, 697, 1230, 1495
BstV2I	GAAGAC	1 cut(s)	94
BstZI	CGGCCG	1 cut(s)	1130
BsuRI	GGCC	2 cut(s)	1132, 1499
BtgI	CCRYGG	2 cut(s)	894, 1133
BtgZI	GCGATG	1 cut(s)	130
BtrI	CACGTC	2 cut(s)	136, 636
BtsCI	GGATG	3 cut(s)	424, 1062, 1515
BtsIMutI	CAGTG	2 cut(s)	773, 794
Cac8I	GCNNGC	2 cut(s)	1340, 1488
CaiI	CAGNNNCTG	1 cut(s)	696
CciI	TCATGA	1 cut(s)	1152
CfoI	GCGC	1 cut(s)	1280
Cfr10I	RCCGGY	2 cut(s)	433, 1128
Cfr13I	GGNCC	2 cut(s)	1200, 1333
Cfr9I	CCCGGG	1 cut(s)	1532
CpoI	CGGWCCG	1 cut(s)	1333
CseI	GACGC	2 cut(s)	1009, 1483
Csp6I	GTAC	3 cut(s)	56, 667, 1141
CspI	CGGWCCG	1 cut(s)	1333
CviQI	GTAC	3 cut(s)	56, 667, 1141
DdeI	CTNAG	6 cut(s)	278, 355, 498, 813, 1167, 1441
DpnI	GATC	8 cut(s)	378, 441, 471, 576, 945, 1098, 1124, 1191
DpnII	GATC	8 cut(s)	376, 439, 469, 574, 943, 1096, 1122, 1189
EaeI	YGGCCR	2 cut(s)	1130, 1497
EagI	CGGCCG	1 cut(s)	1130
Eam1104I	CTCTTC	5 cut(s)	113, 501, 507, 833, 1236
EarI	CTCTTC	5 cut(s)	113, 501, 507, 833, 1236
Ecl136II	GAGCTC	1 cut(s)	282
EclXI	CGGCCG	1 cut(s)	1130
Eco24I	GRGCYC	1 cut(s)	284
Eco47I	GGWCC	2 cut(s)	1200, 1333
Eco52I	CGGCCG	1 cut(s)	1130
Eco53kI	GAGCTC	1 cut(s)	282
Eco57I	CTGAAG	1 cut(s)	1198
Eco88I	CYCGRG	3 cut(s)	163, 283, 1532
EcoICRI	GAGCTC	1 cut(s)	282
EcoRII	CCWGG	2 cut(s)	186, 843
EcoT38I	GRGCYC	1 cut(s)	284
FaqI	GGGAC	1 cut(s)	1268
FauI	CCCGC	1 cut(s)	586
FblI	GTMKAC	3 cut(s)	1023, 1389, 1446
Fnu4HI	GCNGC	6 cut(s)	5, 45, 533, 686, 1219, 1484
FokI	GGATG	3 cut(s)	431, 1069, 1502
FriOI	GRGCYC	1 cut(s)	284
Fsp4HI	GCNGC	6 cut(s)	5, 45, 533, 686, 1219, 1484
FspBI	CTAG	2 cut(s)	428, 731
FspI	TGCGCA	1 cut(s)	1279
GlaI	GCGC	1 cut(s)	1279
GluI	GCNGC	6 cut(s)	5, 45, 533, 686, 1219, 1484
GsuI	CTGGAG	2 cut(s)	170, 182
HaeIII	GGCC	2 cut(s)	1132, 1499
HapII	CCGG	9 cut(s)	221, 434, 536, 831, 1129, 1144, 1478, 1496, 1533
HgaI	GACGC	2 cut(s)	1009, 1483
HhaI	GCGC	1 cut(s)	1280
Hin1I	GRCGYC	2 cut(s)	1020, 1475
Hin6I	GCGC	1 cut(s)	1278
HinP1I	GCGC	1 cut(s)	1278
HincII	GTYRAC	2 cut(s)	1024, 1473
HindII	GTYRAC	2 cut(s)	1024, 1473
HinfI	GANTC	9 cut(s)	38, 245, 316, 583, 659, 770, 794, 929, 1149
HpaII	CCGG	9 cut(s)	221, 434, 536, 831, 1129, 1144, 1478, 1496, 1533
HphI	GGTGA	3 cut(s)	578, 859, 1016
Hpy166II	GTNNAC	5 cut(s)	1024, 1177, 1390, 1447, 1473
Hpy8I	GTNNAC	5 cut(s)	1024, 1177, 1390, 1447, 1473
Hpy99I	CGWCG	4 cut(s)	89, 92, 458, 1025
HpyAV	CCTTC	4 cut(s)	46, 645, 649, 1009
HpyCH4III	ACNGT	4 cut(s)	383, 698, 898, 1207
HpyCH4IV	ACGT	3 cut(s)	135, 635, 1570
HpyCH4V	TGCA	5 cut(s)	4, 639, 955, 1433, 1515
HpyF10VI	GCNNNNNNNGC	7 cut(s)	818, 840, 1275, 1335, 1483, 1496, 1505
HpyF3I	CTNAG	6 cut(s)	278, 355, 498, 813, 1167, 1441
HpySE526I	ACGT	3 cut(s)	135, 635, 1570
Hsp92I	GRCGYC	2 cut(s)	1020, 1475
HspAI	GCGC	1 cut(s)	1278
Kzo9I	GATC	8 cut(s)	376, 439, 469, 574, 943, 1096, 1122, 1189
LguI	GCTCTTC	1 cut(s)	833
LmnI	GCTCC	3 cut(s)	625, 863, 1480
Lsp1109I	GCAGC	4 cut(s)	16, 697, 1230, 1495
LweI	GCATC	2 cut(s)	1420, 1524
MaeI	CTAG	2 cut(s)	428, 731
MaeII	ACGT	3 cut(s)	135, 635, 1570
MaeIII	GTNAC	1 cut(s)	631
MalI	GATC	8 cut(s)	378, 441, 471, 576, 945, 1098, 1124, 1191
MbiI	CCGCTC	1 cut(s)	1584
MboI	GATC	8 cut(s)	376, 439, 469, 574, 943, 1096, 1122, 1189
MhlI	GDGCHC	4 cut(s)	284, 916, 1331, 1386
MluCI	AATT	9 cut(s)	322, 412, 482, 737, 902, 934, 990, 1009, 1032
MlyI	GAGTC	5 cut(s)	239, 577, 653, 938, 1158
MmeI	TCCRAC	4 cut(s)	321, 351, 381, 745
MroXI	GAANNNNTTC	1 cut(s)	1064
MseI	TTAA	3 cut(s)	714, 965, 993
MspI	CCGG	9 cut(s)	221, 434, 536, 831, 1129, 1144, 1478, 1496, 1533
MspR9I	CCNGG	7 cut(s)	188, 221, 831, 845, 1496, 1533, 1534
Mva1269I	GAATGC	1 cut(s)	614
MvaI	CCWGG	2 cut(s)	188, 845
MvnI	CGCG	1 cut(s)	581
MwoI	GCNNNNNNNGC	7 cut(s)	818, 840, 1275, 1335, 1483, 1496, 1505
NciI	CCSGG	5 cut(s)	221, 831, 1496, 1533, 1534
NdeII	GATC	8 cut(s)	376, 439, 469, 574, 943, 1096, 1122, 1189
NlaIV	GGNNCC	3 cut(s)	859, 915, 1526
NmeAIII	GCCGAG	1 cut(s)	175
NmuCI	GTSAC	1 cut(s)	631
NsbI	TGCGCA	1 cut(s)	1279
NspI	RCATGY	2 cut(s)	955, 1225
PaeR7I	CTCGAG	2 cut(s)	163, 283
PagI	TCATGA	1 cut(s)	1152
PciSI	GCTCTTC	1 cut(s)	833
PcsI	WCGNNNNNNNCGW	1 cut(s)	1122
PctI	GAATGC	1 cut(s)	614
PdmI	GAANNNNTTC	1 cut(s)	1064
PfeI	GAWTC	4 cut(s)	38, 316, 770, 794
PfoI	TCCNGGA	1 cut(s)	186
PkrI	GCNGC	6 cut(s)	6, 46, 534, 687, 1220, 1485
PleI	GAGTC	5 cut(s)	239, 577, 653, 937, 1157
PpsI	GAGTC	5 cut(s)	239, 577, 653, 937, 1157
Ppu21I	YACGTR	1 cut(s)	1571
Psp124BI	GAGCTC	1 cut(s)	284
Psp6I	CCWGG	2 cut(s)	186, 843
PspGI	CCWGG	2 cut(s)	186, 843
PspN4I	GGNNCC	3 cut(s)	859, 915, 1526
PspPI	GGNCC	2 cut(s)	1200, 1333
PstNI	CAGNNNCTG	1 cut(s)	696
RsaI	GTAC	3 cut(s)	57, 668, 1142
RsaNI	GTAC	3 cut(s)	56, 667, 1141
Rsr2I	CGGWCCG	1 cut(s)	1333
RsrII	CGGWCCG	1 cut(s)	1333
SacI	GAGCTC	1 cut(s)	284
SalI	GTCGAC	1 cut(s)	1022
SapI	GCTCTTC	1 cut(s)	833
SaqAI	TTAA	3 cut(s)	714, 965, 993
SatI	GCNGC	6 cut(s)	5, 45, 533, 686, 1219, 1484
Sau3AI	GATC	8 cut(s)	376, 439, 469, 574, 943, 1096, 1122, 1189
Sau96I	GGNCC	2 cut(s)	1200, 1333
SchI	GAGTC	5 cut(s)	239, 577, 653, 938, 1158
ScrFI	CCNGG	7 cut(s)	188, 221, 831, 845, 1496, 1533, 1534
SduI	GDGCHC	4 cut(s)	284, 916, 1331, 1386
SfaNI	GCATC	2 cut(s)	1420, 1524
Sfr274I	CTCGAG	2 cut(s)	163, 283
SinI	GGWCC	2 cut(s)	1200, 1333
SlaI	CTCGAG	2 cut(s)	163, 283
SmaI	CCCGGG	1 cut(s)	1534
SmlI	CTYRAG	3 cut(s)	163, 177, 283
SmoI	CTYRAG	3 cut(s)	163, 177, 283
Sse9I	AATT	9 cut(s)	322, 412, 482, 737, 902, 934, 990, 1009, 1032
SsiI	CCGC	6 cut(s)	44, 533, 579, 1336, 1423, 1582
SspMI	CTAG	2 cut(s)	428, 731
SstI	GAGCTC	1 cut(s)	284
StyD4I	CCNGG	7 cut(s)	186, 219, 829, 843, 1494, 1531, 1532
TaaI	ACNGT	4 cut(s)	383, 698, 898, 1207
TaiI	ACGT	3 cut(s)	138, 638, 1573
TaqI	TCGA	9 cut(s)	164, 206, 284, 456, 573, 970, 1023, 1095, 1125
TaqII	GACCGA	2 cut(s)	88, 257
TasI	AATT	9 cut(s)	322, 412, 482, 737, 902, 934, 990, 1009, 1032
TauI	GCSGC	2 cut(s)	47, 535
TfiI	GAWTC	4 cut(s)	38, 316, 770, 794
Tru1I	TTAA	3 cut(s)	714, 965, 993
Tru9I	TTAA	3 cut(s)	714, 965, 993
TscAI	CASTG	2 cut(s)	780, 794
TseFI	GTSAC	1 cut(s)	631
TseI	GCWGC	4 cut(s)	4, 685, 1218, 1483
Tsp45I	GTSAC	1 cut(s)	631
TspDTI	ATGAA	7 cut(s)	65, 109, 435, 1073, 1088, 1169, 1391
TspGWI	ACGGA	4 cut(s)	573, 638, 1124, 1346
TspMI	CCCGGG	1 cut(s)	1532
TspRI	CASTG	2 cut(s)	780, 794
VpaK11BI	GGWCC	2 cut(s)	1200, 1333
XapI	RAATTY	2 cut(s)	902, 934
XceI	RCATGY	2 cut(s)	955, 1225
XhoI	CTCGAG	2 cut(s)	163, 283
XmaI	CCCGGG	1 cut(s)	1532
XmiI	GTMKAC	3 cut(s)	1023, 1389, 1446
XmnI	GAANNNNTTC	1 cut(s)	1064
XspI	CTAG	2 cut(s)	428, 731

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.