FvH4_7g33530

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002642
Physical Location & Seq
Forward (+)
23831634 .. 23834379
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33530.t1

Sequence Viewer

Length: 1545 bp
ATGCTGACGCAGTCCATTTTGTGTCCCACGTCACCTTCACTGTCATCATCCTCAGGTAGCTTCTTCACTTCTCTCTCTTTGCTCTGCCCAACTTGTCTCAAACACTCTCACAAACACAAGCTCCATGCTTCTCTGACTCACTCAAATCCCAACTTTCTAGCTTTAAACGACCAAACTCATTCAATTTCTGACCACCCAAATGGCCCGAGTATAGGAGAGGAGATTTCTTTTGAGTTATACAACAGTTCGTTACAGGACTTTTGTAGAGCTGGGCATGTTGACAAGGCCATGGCTCTTCTTGCTCAAATGGAAGCTATTGGATTAAGACCCAATTCGGTATCGTATTCTTATATGATTGATGCTTTAGGAAGTATAGGCAGGACTTTGGAGGCTGATGTATTGTTTCAGGAGATGATATTTCTGGGGTATAGGCCAAGAATAAAGCTTTACAATGTTTTGCTTAGAGGGTTTTTGAAGAAAGGTTTGTTAGGACTTGCAGTTAGAGTTTTGGGGGTGATGGGTGATTTGGGTGCTGAGAGAAATCAAGAAACATATGAGATTTTACTTGATTACTATGTCAGTGCTGGGAGGTTGGAGGATACTTGGTCAATGATTAATGAGATGAGGAGGAAGAGGTTTCGGCTGAGCTCGTTTGTGTATAGTAAGGTTATTGGTCTGTATAGGGACAATGGGATGTGGAAGAAGGCGGTGGACATTGTGGAAGAGATAAGGGAAATGGGGATGGCATTGGACAAACAGATTTACAACAGCATTATCGATACGTTTGGGAAGTATGGGGAGTTGGATGAAGCTTTGGAAGTGTTTGGGAGAATGAAAGAAGAAGGTGTGAGGCCTGATATTTCGACCTTTAATTCGTTGATAAGGTGGCACTGCAAGTCTGGGTTGTTAAGGAAGGCCCTCGAGTTGTTTACTGAGATGCAAGAACAAGGATTATATCCTGACCCGAAAATATTTGTTACTATTATCAGTAGATTGGGGGAGCAAGGGAAGTGGGATATGATACGGAGGACCTTTGAGAATATGAGAAGCCGTGGGCATAAAAAAAGTGGGACTATTTATGCTGCTCTCGTTGACATTTATGGGCAATATGGAAAATTTCAGGACGCAGAAGAGTGTATATCTGCACTACAGGCAGAAGGTCTTGTTATCTCTGCCAGTATGTTTTGTGTATTAGCAAATGCATACGCCCAGCAGGGATTATGTGATCAGACAGTTAAAGTACTTCAGCTCATGGAAGCTGAGGGAATTGAACCAAATGTTATAATGCTGAATGTTTTGATCAATGCATTTGGTATTGCTGGTAGACATTTGGAGGCTTTGTCCATTTATCACCATATAAAAGAAAGTGGTTTAAGCCCTGATGTAGTTACGTATACTACCCTTATGAAGGCATTTATTCGAGCAAGGAAGTATGATAGGGTCCCTGAGATATACATTGATATGGAACAGGCTGGATGCACCCCAGATAGGAAGGCCAGACAGATGTTAGAAGTCGCTTCATTGGCCCTTCAACAGAGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

515

Amino Acids

57.97

Weight (kDa)

6.69

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 80 - 122 2.1e-09 PPR repeat family
TPR_24 PF23276 160 - 275 2.8e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 181 - 224 2.1e-06 PPR repeat family
TPR_24 PF23276 229 - 338 1.1e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 239 - 295 9.9e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 247 - 279 1e-05 PPR repeat
PPR_2 PF13041 254 - 299 1.3e-12 PPR repeat family
PPR PF01535 254 - 283 2.7e-07 PPR repeat
PPR_1 PF12854 282 - 314 2e-09 PPR repeat
PPR_2 PF13041 285 - 321 2.8e-10 PPR repeat family
PPR PF01535 288 - 318 1.7e-08 PPR repeat
TPR_24 PF23276 328 - 452 6.9e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 383 - 438 1.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 425 - 473 1.3e-11 PPR repeat family
PPR_3 PF13812 448 - 496 9.6e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 460 - 497 5.8e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1283
AccI GTMKAC 2 cut(s) 1324, 1394
AciI CCGC 1 cut(s) 707
AcsI RAATTY 1 cut(s) 1115
AcuI CTGAAG 1 cut(s) 1229
AfaI GTAC 1 cut(s) 1242
AfiI CCNNNNNNNGG 3 cut(s) 212, 1408, 1488
AgsI TTSAA 4 cut(s) 183, 475, 1271, 1532
AjiI CACGTC 1 cut(s) 30
Alw21I GWGCWC 1 cut(s) 650
Alw26I GTCTC 1 cut(s) 101
Ama87I CYCGRG 2 cut(s) 205, 920
AoxI GGCC 7 cut(s) 202, 285, 431, 851, 915, 1494, 1524
ApeKI GCWGC 1 cut(s) 1082
ApoI RAATTY 1 cut(s) 1115
ArsI GACNNNNNNTTYG 2 cut(s) 856, 888
AseI ATTAAT 1 cut(s) 615
AspS9I GGNCC 5 cut(s) 203, 916, 1029, 1441, 1525
AsuHPI GGTGA 4 cut(s) 24, 526, 533, 1343
AvaI CYCGRG 2 cut(s) 205, 920
AvaII GGWCC 2 cut(s) 1029, 1441
AxyI CCTNAGG 1 cut(s) 52
BanII GRGCYC 1 cut(s) 650
Bbv12I GWGCWC 1 cut(s) 650
BbvCI CCTCAGC 1 cut(s) 1260
BbvI GCAGC 1 cut(s) 1069
BccI CCATC 2 cut(s) 511, 736
BceAI ACGGC 1 cut(s) 1035
BciVI GTATCC 1 cut(s) 592
BclI TGATCA 2 cut(s) 1225, 1299
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 1148
BfuI GTATCC 1 cut(s) 592
BisI GCNGC 1 cut(s) 1083
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 1 cut(s) 1084
BmcAI AGTACT 1 cut(s) 1242
Bme18I GGWCC 2 cut(s) 1029, 1441
BmeT110I CYCGRG 2 cut(s) 205, 920
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 5 cut(s) 203, 916, 1029, 1441, 1525
BmiI GGNNCC 2 cut(s) 1442, 1443
BmsI GCATC 3 cut(s) 349, 927, 1466
Bpu10I CCTNAGC 1 cut(s) 1260
Bpu1102I GCTNAGC 1 cut(s) 644
Bsa29I ATCGAT 1 cut(s) 777
BsaAI YACGTR 1 cut(s) 1392
BsaJI CCNNGG 2 cut(s) 288, 1051
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 1325, 1355
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 1408, 1488
Bse1I ACTGG 1 cut(s) 1176
Bse21I CCTNAGG 1 cut(s) 52
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 2 cut(s) 288, 1051
BseGI GGATG 5 cut(s) 47, 699, 747, 811, 1481
BseLI CCNNNNNNNGG 3 cut(s) 212, 1408, 1488
BseMII CTCAG 6 cut(s) 66, 525, 635, 924, 1251, 1437
BseNI ACTGG 1 cut(s) 1176
BseRI GAGGAG 2 cut(s) 233, 640
BseXI GCAGC 1 cut(s) 1069
BseYI CCCAGC 3 cut(s) 269, 584, 1209
BsgI GTGCAG 1 cut(s) 1128
BshFI GGCC 7 cut(s) 204, 287, 433, 853, 917, 1496, 1526
BshVI ATCGAT 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 650
BsiHKCI CYCGRG 2 cut(s) 205, 920
BslFI GGGAC 4 cut(s) 9, 698, 1084, 1427
BslI CCNNNNNNNGG 3 cut(s) 212, 1408, 1488
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 4 cut(s) 9, 698, 1084, 1427
BsnI GGCC 7 cut(s) 204, 287, 433, 853, 917, 1496, 1526
BsoBI CYCGRG 2 cut(s) 205, 920
Bsp1286I GDGCHC 1 cut(s) 650
Bsp143I GATC 2 cut(s) 1225, 1299
Bsp1720I GCTNAGC 1 cut(s) 644
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 1 cut(s) 707
BspANI GGCC 7 cut(s) 204, 287, 433, 853, 917, 1496, 1526
BspCNI CTCAG 6 cut(s) 65, 526, 636, 925, 1252, 1438
BspDI ATCGAT 1 cut(s) 777
BspLI GGNNCC 2 cut(s) 1442, 1443
BspQI GCTCTTC 1 cut(s) 300
BsrI ACTGG 1 cut(s) 1176
BssECI CCNNGG 2 cut(s) 288, 1051
BssMI GATC 2 cut(s) 1225, 1299
BssNAI GTATAC 1 cut(s) 1395
BssT1I CCWWGG 1 cut(s) 288
Bst1107I GTATAC 1 cut(s) 1395
Bst4CI ACNGT 3 cut(s) 42, 245, 1234
Bst6I CTCTTC 4 cut(s) 300, 626, 717, 1125
BstBAI YACGTR 1 cut(s) 1392
BstDEI CTNAG 7 cut(s) 52, 461, 534, 644, 933, 1260, 1446
BstDSI CCRYGG 2 cut(s) 288, 1051
BstENI CCTNNNNNAGG 1 cut(s) 1406
BstF5I GGATG 5 cut(s) 47, 699, 747, 811, 1481
BstKTI GATC 2 cut(s) 1228, 1302
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 1225, 1299
BstMWI GCNNNNNNNGC 3 cut(s) 299, 1151, 1523
BstNSI RCATGY 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 1148
BstSNI TACGTA 1 cut(s) 1392
BstV1I GCAGC 1 cut(s) 1069
BstXI CCANNNNNNTGG 1 cut(s) 200
BstZ17I GTATAC 1 cut(s) 1395
Bsu15I ATCGAT 1 cut(s) 777
Bsu36I CCTNAGG 1 cut(s) 52
BsuI GTATCC 1 cut(s) 592
BsuRI GGCC 7 cut(s) 204, 287, 433, 853, 917, 1496, 1526
BsuTUI ATCGAT 1 cut(s) 777
BtgI CCRYGG 2 cut(s) 288, 1051
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 5 cut(s) 47, 699, 747, 811, 1481
BtsI GCAGTG 1 cut(s) 889
BtsIMutI CAGTG 3 cut(s) 38, 586, 889
Cfr13I GGNCC 5 cut(s) 203, 916, 1029, 1441, 1525
ClaI ATCGAT 1 cut(s) 777
CseI GACGC 2 cut(s) 16, 1133
Csp6I GTAC 1 cut(s) 1241
CspCI CAANNNNNGTGG 2 cut(s) 992, 1027
CviAII CATG 4 cut(s) 125, 275, 289, 1252
CviQI GTAC 1 cut(s) 1241
DdeI CTNAG 7 cut(s) 52, 461, 534, 644, 933, 1260, 1446
DpnI GATC 2 cut(s) 1227, 1301
DpnII GATC 2 cut(s) 1225, 1299
DraI TTTAAA 1 cut(s) 165
Eam1104I CTCTTC 4 cut(s) 300, 626, 717, 1125
EarI CTCTTC 4 cut(s) 300, 626, 717, 1125
Ecl136II GAGCTC 1 cut(s) 648
Eco105I TACGTA 1 cut(s) 1392
Eco130I CCWWGG 1 cut(s) 288
Eco147I AGGCCT 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 650
Eco47I GGWCC 2 cut(s) 1029, 1441
Eco53kI GAGCTC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 1229
Eco81I CCTNAGG 1 cut(s) 52
Eco88I CYCGRG 2 cut(s) 205, 920
EcoICRI GAGCTC 1 cut(s) 648
EcoNI CCTNNNNNAGG 1 cut(s) 1406
EcoO109I RGGNCCY 3 cut(s) 916, 1029, 1441
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 2 cut(s) 1204, 1309
EcoT38I GRGCYC 1 cut(s) 650
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 4 cut(s) 128, 278, 292, 1255
FaqI GGGAC 4 cut(s) 9, 698, 1084, 1427
FatI CATG 4 cut(s) 124, 274, 288, 1251
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 2 cut(s) 1225, 1299
FblI GTMKAC 2 cut(s) 1324, 1394
Fnu4HI GCNGC 1 cut(s) 1083
FokI GGATG 5 cut(s) 34, 706, 754, 818, 1488
FriOI GRGCYC 1 cut(s) 650
Fsp4HI GCNGC 1 cut(s) 1083
FspBI CTAG 1 cut(s) 158
GluI GCNGC 1 cut(s) 1083
GsaI CCCAGC 3 cut(s) 273, 588, 1213
HaeIII GGCC 7 cut(s) 204, 287, 433, 853, 917, 1496, 1526
HgaI GACGC 2 cut(s) 16, 1133
Hin1II CATG 4 cut(s) 128, 278, 292, 1255
HincII GTYRAC 2 cut(s) 280, 1093
HindII GTYRAC 2 cut(s) 280, 1093
HindIII AAGCTT 2 cut(s) 443, 810
HinfI GANTC 1 cut(s) 136
HphI GGTGA 4 cut(s) 24, 526, 533, 1343
Hpy166II GTNNAC 6 cut(s) 280, 712, 930, 1093, 1325, 1395
Hpy188I TCNGA 3 cut(s) 135, 190, 1230
Hpy188III TCNNGA 4 cut(s) 407, 545, 959, 1121
Hpy8I GTNNAC 6 cut(s) 280, 712, 930, 1093, 1325, 1395
HpyAV CCTTC 8 cut(s) 45, 697, 836, 907, 1151, 1402, 1486, 1538
HpyCH4III ACNGT 3 cut(s) 42, 245, 1234
HpyCH4IV ACGT 3 cut(s) 29, 782, 1391
HpyCH4V TGCA 7 cut(s) 497, 894, 940, 1145, 1202, 1307, 1479
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 1151, 1523
HpyF3I CTNAG 7 cut(s) 52, 461, 534, 644, 933, 1260, 1446
HpySE526I ACGT 3 cut(s) 29, 782, 1391
Hsp92II CATG 4 cut(s) 128, 278, 292, 1255
KflI GGGWCCC 1 cut(s) 1441
Ksp22I TGATCA 2 cut(s) 1225, 1299
Kzo9I GATC 2 cut(s) 1225, 1299
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 2 cut(s) 126, 1000
Lsp1109I GCAGC 1 cut(s) 1069
LweI GCATC 3 cut(s) 349, 927, 1466
MaeI CTAG 1 cut(s) 158
MaeII ACGT 3 cut(s) 29, 782, 1391
MaeIII GTNAC 4 cut(s) 30, 249, 976, 1387
MalI GATC 2 cut(s) 1227, 1301
MboI GATC 2 cut(s) 1225, 1299
MboII GAAGA 8 cut(s) 55, 287, 487, 643, 712, 734, 851, 1142
MhlI GDGCHC 1 cut(s) 650
MluCI AATT 6 cut(s) 183, 331, 871, 1115, 1266, 1540
MlyI GAGTC 1 cut(s) 130
MmeI TCCRAC 2 cut(s) 573, 783
Mph1103I ATGCAT 2 cut(s) 1204, 1309
MseI TTAA 7 cut(s) 164, 323, 615, 870, 908, 1236, 1373
MslI CAYNNNNRTG 2 cut(s) 198, 1460
MwoI GCNNNNNNNGC 3 cut(s) 299, 1151, 1523
NcoI CCATGG 1 cut(s) 288
NdeI CATATG 1 cut(s) 553
NdeII GATC 2 cut(s) 1225, 1299
NlaIII CATG 4 cut(s) 128, 278, 292, 1255
NlaIV GGNNCC 2 cut(s) 1442, 1443
NmuCI GTSAC 1 cut(s) 30
NsiI ATGCAT 2 cut(s) 1204, 1309
NspI RCATGY 1 cut(s) 278
PaeR7I CTCGAG 1 cut(s) 920
PceI AGGCCT 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 300
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 1084
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
Ppu21I YACGTR 1 cut(s) 1392
PpuMI RGGWCCY 2 cut(s) 1029, 1441
PshBI ATTAAT 1 cut(s) 615
PsiI TTATAA 1 cut(s) 1283
Psp124BI GAGCTC 1 cut(s) 650
Psp5II RGGWCCY 2 cut(s) 1029, 1441
PspFI CCCAGC 3 cut(s) 269, 584, 1209
PspN4I GGNNCC 2 cut(s) 1442, 1443
PspPI GGNCC 5 cut(s) 203, 916, 1029, 1441, 1525
PspPPI RGGWCCY 2 cut(s) 1029, 1441
PspXI VCTCGAGB 1 cut(s) 920
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 1 cut(s) 1242
RsaNI GTAC 1 cut(s) 1241
RseI CAYNNNNRTG 2 cut(s) 198, 1460
SacI GAGCTC 1 cut(s) 650
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 7 cut(s) 164, 323, 615, 870, 908, 1236, 1373
SatI GCNGC 1 cut(s) 1083
Sau3AI GATC 2 cut(s) 1225, 1299
Sau96I GGNCC 5 cut(s) 203, 916, 1029, 1441, 1525
ScaI AGTACT 1 cut(s) 1242
SchI GAGTC 1 cut(s) 130
SduI GDGCHC 1 cut(s) 650
SfaNI GCATC 3 cut(s) 349, 927, 1466
SfcI CTRYAG 1 cut(s) 1148
Sfr274I CTCGAG 1 cut(s) 920
SinI GGWCC 2 cut(s) 1029, 1441
SlaI CTCGAG 1 cut(s) 920
SmiMI CAYNNNNRTG 2 cut(s) 198, 1460
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SnaBI TACGTA 1 cut(s) 1392
Sse9I AATT 6 cut(s) 183, 331, 871, 1115, 1266, 1540
SseBI AGGCCT 1 cut(s) 853
SsiI CCGC 1 cut(s) 707
SspI AATATT 1 cut(s) 972
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 650
StuI AGGCCT 1 cut(s) 853
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 3 cut(s) 42, 245, 1234
TaiI ACGT 3 cut(s) 32, 785, 1394
TaqI TCGA 4 cut(s) 777, 863, 921, 1420
TasI AATT 6 cut(s) 183, 331, 871, 1115, 1266, 1540
TatI WGTACW 1 cut(s) 1240
Tru1I TTAA 7 cut(s) 164, 323, 615, 870, 908, 1236, 1373
Tru9I TTAA 7 cut(s) 164, 323, 615, 870, 908, 1236, 1373
TscAI CASTG 3 cut(s) 45, 586, 896
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 1 cut(s) 1082
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 4 cut(s) 822, 848, 1421, 1509
TspGWI ACGGA 1 cut(s) 1039
TspRI CASTG 3 cut(s) 45, 586, 896
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 1029, 1441
VspI ATTAAT 1 cut(s) 615
XagI CCTNNNNNAGG 1 cut(s) 1406
XapI RAATTY 1 cut(s) 1115
XceI RCATGY 1 cut(s) 278
XhoI CTCGAG 1 cut(s) 920
XmiI GTMKAC 2 cut(s) 1324, 1394
XspI CTAG 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 1242
Zsp2I ATGCAT 2 cut(s) 1204, 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.