pycom01g24130

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002642
Physical Location & Seq
Forward (+)
21166586 .. 21168705
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g24130.1

Sequence Viewer

Length: 1719 bp
ATGTCACACAATTTCATTAAAGTCAAATCCAACCTAAAGAATTCCGTTGTTGGAATATGTCTAGGACTCGATCCAAGACACCTGGTTGGGTCAGCGTCCTATCTAACCGGTCCTATAAAAGCAAACAAAAACACTGAAATCTTTGGCGCCTATGAGAGCATGGCGATGCTGGTGCAGTCCATTTGGTGTCGCAACTCACCTTCACTTTCATCAGCAGGCAGCTTCGCAATCACTCCACTCTCTCTCTTCTGTCTGAGATGCCTCAAACACTCCGAGACGTACAATTTCCACGCTTCTTTCACTCACTCGAATCGTACCAAGAACGCGAAAGATTTCAAATTTACAGCATCCAATGACGAAAACACTTCAATTCCAAGCTACCTAATTGCTCAAACGACCGGAGAGGAACTTTCCGGCGAATCATACAACAATTTGATAAGTGGGTTCTGCAGAGCAGGGCATATCGACCAGGCAATGACTGTTCTTGAAGAAATGGAAGCTCTCGGCGTCCGACCCAATTCGATGTCTTACGCTCATTTGATTGAGGGCCTCGGGAGCAATGGCAGGACTTTGGAGGCCGATGTGTTGTTTCAGGAGATGATATGTTCTGGGTTGAGGCCAAGAATCAGGGTTTACAATGTTATGCTCAGAGGGTGTTTGAAGAAAGGCCTCTTAGAACTTGCAACTAGAGTTTTGGCTGTAATGGGCGATTTGGTTGCGGAGAAAAATGAAGAGACGTATGAGATTCTTCTTGATTACTATGTCAGTTCCGGGAGGTTGGAAGATACTTGGTCAACGATTAATGAGATGAAGCGCAAGCGGTTTCGGTTGAGCTCGTTTGTGTATAGCAAAGTTATTGGACTTTACAGGGACAATGGGATGTGGAAGAAGGCAATGGACATTGTGGGAGAGATAAGGGAGATGGGGATGGCATTGGATAAACAGATTTTCAACAGCATTATTGATACGTTTGGGAAATATGGTGAATTGGACGAAGCTTTGGAAGTATTTGATAAGATGAAACAAGAAGGTGTGAAGCCTGATATAATGACGTTTAATTCATTGATACGGTGGCATTGTAAGGCAGGGAATATAAGCAAGGCACTTGAGTTGTTTACAGAGATGCAGGAACAGGGGTTGTATCCTGATCCAAAGATCTGTGTCACCGTTATCAGCCGATTGGGGGAGCAGGGGAAGTGGGATGTGATCCAGAAGACTTTTGAGAATATGAAACGCCGAGGGCATAAGAAAAGTGGGATAATTTATGCTGTTTTGGTTGATATTTATGGGCAGTATGGAAAATTTCAGGATGCTGAAGAGTGTATATTTGCCCTAAAAGCTGAAGGTCTTATTCCTTCAGCTAGCATGTTTTGTGTCTTAGCAAATGCCTACGCTCAGCAGGGATTATGTCATCAGACAGTTAAGGTACTCCAGCTTATGGAAGCTGAGGGAATCGAACCAAACGTCATAATGCTGAATGTTCTGATTAATGCATTCGGTGTTGCTGGTAGATATTTAGAGGCATTATCCATTTATCACCATATAAAAGAAAGTGGTTTTAGCCCTGATGTGGTTACTTATACTACACTTATGAAGGCGTTTATTCGGGCAAGGAAGTTTGATAAGGTCCTGGAGATATACAAGGAAATGGAACGTGCCGGATGCACTCCGGATAGAAAGGCTAGACAGATGTTACAAGTTGCTTTAATGGTCCTTTAA

Protein Analysis

573

Amino Acids

64.36

Weight (kDa)

8.09

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 117 - 272 1.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 134 - 185 2.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 141 - 184 2.4e-11 PPR repeat family
PPR_1 PF12854 141 - 166 3.6e-09 PPR repeat
PPR PF01535 141 - 170 1.5e-07 PPR repeat
PPR_2 PF13041 172 - 221 7.2e-08 PPR repeat family
TPR_24 PF23276 217 - 339 3.3e-10 Fungal tetratrico peptide repeats
PPR_2 PF13041 243 - 286 1.7e-06 PPR repeat family
PPR_long PF17177 269 - 382 4.4e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 301 - 357 5.3e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 309 - 341 2e-06 PPR repeat
PPR PF01535 316 - 345 8.1e-07 PPR repeat
PPR_2 PF13041 316 - 357 1.1e-11 PPR repeat family
PPR_1 PF12854 344 - 376 9.5e-11 PPR repeat
PPR_2 PF13041 347 - 383 1.1e-10 PPR repeat family
PPR PF01535 351 - 380 3.3e-08 PPR repeat
TPR_24 PF23276 386 - 514 7.7e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 446 - 500 7.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 487 - 535 1e-11 PPR repeat family
PPR_3 PF13812 510 - 558 2.7e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 522 - 559 2.1e-10 PPR repeat family
PPR PF01535 525 - 555 1.5e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 1438
AccII CGCG 1 cut(s) 326
AccIII TCCGGA 1 cut(s) 1669
AciI CCGC 2 cut(s) 719, 820
AclWI GGATC 3 cut(s) 65, 1142, 1201
AcsI RAATTY 3 cut(s) 40, 338, 1301
AcuI CTGAAG 3 cut(s) 1335, 1341, 1362
AcyI GRCGYC 2 cut(s) 147, 507
AfaI GTAC 3 cut(s) 281, 316, 1428
AfiI CCNNNNNNNGG 3 cut(s) 1183, 1438, 1570
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 5 cut(s) 337, 369, 488, 661, 952
AjnI CCWGG 3 cut(s) 81, 468, 1629
AluBI AGCT 9 cut(s) 222, 378, 500, 834, 998, 1340, 1361, 1435, 1445
AluI AGCT 9 cut(s) 222, 378, 500, 834, 998, 1340, 1361, 1435, 1445
Alw21I GWGCWC 1 cut(s) 836
Alw26I GTCTC 2 cut(s) 269, 728
AlwI GGATC 3 cut(s) 65, 1142, 1201
Ama87I CYCGRG 1 cut(s) 551
Aor13HI TCCGGA 1 cut(s) 1669
AoxI GGCC 4 cut(s) 547, 576, 617, 667
ApeKI GCWGC 1 cut(s) 219
ApoI RAATTY 3 cut(s) 40, 338, 1301
ArsI GACNNNNNNTTYG 2 cut(s) 1449, 1481
AseI ATTAAT 2 cut(s) 801, 1488
AsiGI ACCGGT 1 cut(s) 107
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 2 cut(s) 149, 816
AspS9I GGNCC 4 cut(s) 110, 547, 1627, 1711
AsuC2I CCSGG 1 cut(s) 772
AsuHPI GGTGA 4 cut(s) 189, 995, 1156, 1529
AsuNHI GCTAGC 1 cut(s) 1361
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 3 cut(s) 110, 1627, 1711
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 836
BbsI GAAGAC 1 cut(s) 1220
Bbv12I GWGCWC 1 cut(s) 836
BbvCI CCTCAGC 1 cut(s) 1446
BbvI GCAGC 1 cut(s) 231
BccI CCATC 2 cut(s) 916, 922
BcgI CGANNNNNNTGC 4 cut(s) 154, 188, 698, 732
BciT130I CCWGG 3 cut(s) 83, 470, 1631
BciVI GTATCC 1 cut(s) 1152
BcnI CCSGG 1 cut(s) 772
BcoDI GTCTC 2 cut(s) 269, 728
BfaI CTAG 4 cut(s) 62, 687, 1362, 1683
BfmI CTRYAG 1 cut(s) 448
BfoI RGCGCY 1 cut(s) 150
BfuI GTATCC 1 cut(s) 1152
BglII AGATCT 1 cut(s) 1155
BisI GCNGC 1 cut(s) 220
BlpI GCTNAGC 1 cut(s) 1395
BlsI GCNGC 1 cut(s) 221
Bme1390I CCNGG 4 cut(s) 83, 470, 772, 1631
Bme18I GGWCC 3 cut(s) 110, 1627, 1711
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 4 cut(s) 110, 547, 1627, 1711
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 4 cut(s) 83, 470, 772, 1631
BmsI GCATC 6 cut(s) 156, 248, 356, 1113, 1300, 1652
BmtI GCTAGC 1 cut(s) 1365
BpiI GAAGAC 1 cut(s) 1220
BpmI CTGGAG 2 cut(s) 1415, 1652
Bpu10I CCTNAGC 1 cut(s) 1446
Bpu1102I GCTNAGC 1 cut(s) 1395
BpuEI CTTGAG 1 cut(s) 1127
BpuMI CCSGG 1 cut(s) 772
BsaHI GRCGYC 2 cut(s) 147, 507
BsaJI CCNNGG 2 cut(s) 550, 1237
BsaWI WCCGGW 3 cut(s) 107, 398, 1669
Bsc4I CCNNNNNNNGG 3 cut(s) 1183, 1438, 1570
Bse118I RCCGGY 1 cut(s) 107
Bse3DI GCAATG 3 cut(s) 480, 565, 900
BseAI TCCGGA 1 cut(s) 1669
BseBI CCWGG 3 cut(s) 83, 470, 1631
BseDI CCNNGG 2 cut(s) 550, 1237
BseGI GGATG 6 cut(s) 347, 885, 933, 1207, 1315, 1667
BseLI CCNNNNNNNGG 3 cut(s) 1183, 1438, 1570
BseMI GCAATG 3 cut(s) 480, 565, 900
BseMII CTCAG 4 cut(s) 245, 661, 1409, 1437
BseXI GCAGC 1 cut(s) 231
BsgI GTGCAG 1 cut(s) 194
Bsh1236I CGCG 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 399
BshFI GGCC 4 cut(s) 549, 578, 619, 669
BshNI GGYRCC 1 cut(s) 146
BshTI ACCGGT 1 cut(s) 107
BsiEI CGRYCG 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 836
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 6 cut(s) 108, 399, 414, 771, 1659, 1670
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 3 cut(s) 1183, 1438, 1570
BsmAI GTCTC 2 cut(s) 269, 728
BsmBI CGTCTC 2 cut(s) 269, 728
BsmFI GGGAC 1 cut(s) 884
BsmI GAATGC 1 cut(s) 1493
BsnI GGCC 4 cut(s) 549, 578, 619, 669
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 836
Bsp13I TCCGGA 1 cut(s) 1669
Bsp143I GATC 4 cut(s) 70, 1147, 1155, 1206
Bsp1720I GCTNAGC 1 cut(s) 1395
BspACI CCGC 2 cut(s) 719, 820
BspANI GGCC 4 cut(s) 549, 578, 619, 669
BspCNI CTCAG 4 cut(s) 246, 660, 1408, 1438
BspEI TCCGGA 1 cut(s) 1669
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 1 cut(s) 148
BspMAI CTGCAG 1 cut(s) 452
BspOI GCTAGC 1 cut(s) 1365
BspPI GGATC 3 cut(s) 65, 1142, 1201
BspT107I GGYRCC 1 cut(s) 146
BsrDI GCAATG 3 cut(s) 480, 565, 900
BsrFI RCCGGY 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 550, 1237
BssMI GATC 4 cut(s) 70, 1147, 1155, 1206
BssNI GRCGYC 2 cut(s) 147, 507
Bst2UI CCWGG 3 cut(s) 83, 470, 1631
Bst4CI ACNGT 4 cut(s) 481, 1071, 1168, 1420
Bst6I CTCTTC 3 cut(s) 251, 726, 1311
BstACI GRCGYC 2 cut(s) 147, 507
BstC8I GCNNGC 3 cut(s) 217, 818, 1363
BstDEI CTNAG 6 cut(s) 254, 647, 673, 1378, 1395, 1446
BstF5I GGATG 6 cut(s) 347, 885, 933, 1207, 1315, 1667
BstFNI CGCG 1 cut(s) 326
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 816
BstKTI GATC 4 cut(s) 73, 1150, 1158, 1209
BstMAI GTCTC 2 cut(s) 269, 728
BstMBI GATC 4 cut(s) 70, 1147, 1155, 1206
BstMCI CGRYCG 1 cut(s) 399
BstMWI GCNNNNNNNGC 2 cut(s) 555, 1337
BstNI CCWGG 3 cut(s) 83, 470, 1631
BstNSI RCATGY 1 cut(s) 1369
BstSCI CCNGG 4 cut(s) 81, 468, 770, 1629
BstSFI CTRYAG 1 cut(s) 448
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 1 cut(s) 231
BstV2I GAAGAC 1 cut(s) 1220
BstX2I RGATCY 1 cut(s) 1155
BstYI RGATCY 1 cut(s) 1155
BsuI GTATCC 1 cut(s) 1152
BsuRI GGCC 4 cut(s) 549, 578, 619, 669
BtgZI GCGATG 1 cut(s) 179
BtsCI GGATG 6 cut(s) 347, 885, 933, 1207, 1315, 1667
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 3 cut(s) 217, 818, 1363
CfoI GCGC 2 cut(s) 149, 816
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 4 cut(s) 110, 547, 1627, 1711
CseI GACGC 2 cut(s) 84, 496
CsiI ACCWGGT 1 cut(s) 81
Csp6I GTAC 3 cut(s) 280, 315, 1427
CspAI ACCGGT 1 cut(s) 107
CviAII CATG 2 cut(s) 160, 1366
CviQI GTAC 3 cut(s) 280, 315, 1427
DdeI CTNAG 6 cut(s) 254, 647, 673, 1378, 1395, 1446
DinI GGCGCC 1 cut(s) 148
DpnI GATC 4 cut(s) 72, 1149, 1157, 1208
DpnII GATC 4 cut(s) 70, 1147, 1155, 1206
Eam1104I CTCTTC 3 cut(s) 251, 726, 1311
EarI CTCTTC 3 cut(s) 251, 726, 1311
Ecl136II GAGCTC 1 cut(s) 834
Eco147I AGGCCT 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 836
Eco47I GGWCC 3 cut(s) 110, 1627, 1711
Eco53kI GAGCTC 1 cut(s) 834
Eco57I CTGAAG 3 cut(s) 1335, 1341, 1362
Eco88I CYCGRG 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 834
EcoO109I RGGNCCY 2 cut(s) 547, 1627
EcoRI GAATTC 1 cut(s) 40
EcoRII CCWGG 3 cut(s) 81, 468, 1629
EcoT22I ATGCAT 1 cut(s) 1495
EcoT38I GRGCYC 1 cut(s) 836
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
Esp3I CGTCTC 2 cut(s) 269, 728
FaeI CATG 2 cut(s) 163, 1369
FaqI GGGAC 1 cut(s) 884
FatI CATG 2 cut(s) 159, 1365
Fnu4HI GCNGC 1 cut(s) 220
FokI GGATG 6 cut(s) 334, 892, 940, 1214, 1322, 1674
FriOI GRGCYC 1 cut(s) 836
Fsp4HI GCNGC 1 cut(s) 220
FspBI CTAG 4 cut(s) 62, 687, 1362, 1683
GlaI GCGC 2 cut(s) 148, 815
GluI GCNGC 1 cut(s) 220
GsuI CTGGAG 2 cut(s) 1415, 1652
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 4 cut(s) 549, 578, 619, 669
HapII CCGG 6 cut(s) 108, 399, 414, 771, 1659, 1670
HgaI GACGC 2 cut(s) 84, 496
HhaI GCGC 2 cut(s) 149, 816
Hin1I GRCGYC 2 cut(s) 147, 507
Hin1II CATG 2 cut(s) 163, 1369
Hin6I GCGC 2 cut(s) 147, 814
HinP1I GCGC 2 cut(s) 147, 814
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 1 cut(s) 996
HinfI GANTC 6 cut(s) 66, 310, 419, 624, 745, 1452
HpaII CCGG 6 cut(s) 108, 399, 414, 771, 1659, 1670
HphI GGTGA 4 cut(s) 189, 995, 1156, 1529
Hpy166II GTNNAC 3 cut(s) 634, 795, 1116
Hpy188I TCNGA 6 cut(s) 255, 274, 512, 650, 1416, 1485
Hpy188III TCNNGA 8 cut(s) 485, 553, 593, 752, 1145, 1210, 1307, 1670
Hpy8I GTNNAC 3 cut(s) 634, 795, 1116
HpyAV CCTTC 6 cut(s) 210, 883, 1022, 1337, 1365, 1588
HpyCH4III ACNGT 4 cut(s) 481, 1071, 1168, 1420
HpyCH4IV ACGT 6 cut(s) 278, 737, 968, 1052, 1464, 1654
HpyCH4V TGCA 6 cut(s) 175, 450, 683, 1126, 1493, 1665
HpyF10VI GCNNNNNNNGC 2 cut(s) 555, 1337
HpyF3I CTNAG 6 cut(s) 254, 647, 673, 1378, 1395, 1446
HpySE526I ACGT 6 cut(s) 278, 737, 968, 1052, 1464, 1654
Hsp92I GRCGYC 2 cut(s) 147, 507
Hsp92II CATG 2 cut(s) 163, 1369
HspAI GCGC 2 cut(s) 147, 814
KasI GGCGCC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 1669
Kzo9I GATC 4 cut(s) 70, 1147, 1155, 1206
LmnI GCTCC 2 cut(s) 555, 1186
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 6 cut(s) 156, 248, 356, 1113, 1300, 1652
MabI ACCWGGT 1 cut(s) 81
MaeI CTAG 4 cut(s) 62, 687, 1362, 1683
MaeII ACGT 6 cut(s) 278, 737, 968, 1052, 1464, 1654
MaeIII GTNAC 4 cut(s) 3, 1162, 1573, 1692
MalI GATC 4 cut(s) 72, 1149, 1157, 1208
MboI GATC 4 cut(s) 70, 1147, 1155, 1206
MboII GAAGA 9 cut(s) 238, 500, 673, 740, 743, 794, 898, 1225, 1328
MflI RGATCY 1 cut(s) 1155
MhlI GDGCHC 1 cut(s) 836
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 1 cut(s) 60
MmeI TCCRAC 4 cut(s) 31, 54, 535, 759
Mph1103I ATGCAT 1 cut(s) 1495
MroI TCCGGA 1 cut(s) 1669
MroXI GAANNNNTTC 1 cut(s) 332
MseI TTAA 7 cut(s) 18, 801, 1056, 1422, 1488, 1706, 1717
MslI CAYNNNNRTG 1 cut(s) 164
MspI CCGG 6 cut(s) 108, 399, 414, 771, 1659, 1670
MspR9I CCNGG 4 cut(s) 83, 470, 772, 1631
Mva1269I GAATGC 1 cut(s) 1493
MvaI CCWGG 3 cut(s) 83, 470, 1631
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 555, 1337
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 1 cut(s) 772
NdeII GATC 4 cut(s) 70, 1147, 1155, 1206
NheI GCTAGC 1 cut(s) 1361
NlaIII CATG 2 cut(s) 163, 1369
NlaIV GGNNCC 1 cut(s) 148
NmeAIII GCCGAG 2 cut(s) 483, 1262
NmuCI GTSAC 2 cut(s) 3, 1162
NsiI ATGCAT 1 cut(s) 1495
NspI RCATGY 1 cut(s) 1369
PceI AGGCCT 1 cut(s) 669
PcsI WCGNNNNNNNCGW 1 cut(s) 1461
PctI GAATGC 1 cut(s) 1493
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 5 cut(s) 310, 419, 624, 745, 1452
PflMI CCANNNNNTGG 1 cut(s) 1438
PfoI TCCNGGA 2 cut(s) 770, 1629
PinAI ACCGGT 1 cut(s) 107
PkrI GCNGC 1 cut(s) 221
PleI GAGTC 1 cut(s) 60
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 60
PpuMI RGGWCCY 1 cut(s) 1627
PshBI ATTAAT 2 cut(s) 801, 1488
Psp124BI GAGCTC 1 cut(s) 836
Psp5II RGGWCCY 1 cut(s) 1627
Psp6I CCWGG 3 cut(s) 81, 468, 1629
PspGI CCWGG 3 cut(s) 81, 468, 1629
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 4 cut(s) 110, 547, 1627, 1711
PspPPI RGGWCCY 1 cut(s) 1627
PstI CTGCAG 1 cut(s) 452
PsuI RGATCY 1 cut(s) 1155
RsaI GTAC 3 cut(s) 281, 316, 1428
RsaNI GTAC 3 cut(s) 280, 315, 1427
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 836
SaqAI TTAA 7 cut(s) 18, 801, 1056, 1422, 1488, 1706, 1717
SatI GCNGC 1 cut(s) 220
Sau3AI GATC 4 cut(s) 70, 1147, 1155, 1206
Sau96I GGNCC 4 cut(s) 110, 547, 1627, 1711
SchI GAGTC 1 cut(s) 60
ScrFI CCNGG 4 cut(s) 83, 470, 772, 1631
SduI GDGCHC 1 cut(s) 836
SexAI ACCWGGT 1 cut(s) 81
SfaNI GCATC 6 cut(s) 156, 248, 356, 1113, 1300, 1652
SfcI CTRYAG 1 cut(s) 448
SfoI GGCGCC 1 cut(s) 148
SinI GGWCC 3 cut(s) 110, 1627, 1711
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 1106
SmoI CTYRAG 1 cut(s) 1106
SseBI AGGCCT 1 cut(s) 669
SsiI CCGC 2 cut(s) 719, 820
SspDI GGCGCC 1 cut(s) 146
SspMI CTAG 4 cut(s) 62, 687, 1362, 1683
SstI GAGCTC 1 cut(s) 836
StuI AGGCCT 1 cut(s) 669
StyD4I CCNGG 4 cut(s) 81, 468, 770, 1629
TaaI ACNGT 4 cut(s) 481, 1071, 1168, 1420
TaiI ACGT 6 cut(s) 281, 740, 971, 1055, 1467, 1657
TaqI TCGA 5 cut(s) 69, 308, 465, 521, 1455
TfiI GAWTC 5 cut(s) 310, 419, 624, 745, 1452
Tru1I TTAA 7 cut(s) 18, 801, 1056, 1422, 1488, 1706, 1717
Tru9I TTAA 7 cut(s) 18, 801, 1056, 1422, 1488, 1706, 1717
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 2 cut(s) 3, 1162
TseI GCWGC 1 cut(s) 219
Tsp45I GTSAC 2 cut(s) 3, 1162
TspDTI ATGAA 8 cut(s) 4, 198, 744, 824, 1034, 1050, 1244, 1607
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 1 cut(s) 139
Van91I CCANNNNNTGG 1 cut(s) 1438
VpaK11BI GGWCC 3 cut(s) 110, 1627, 1711
VspI ATTAAT 2 cut(s) 801, 1488
XapI RAATTY 3 cut(s) 40, 338, 1301
XceI RCATGY 1 cut(s) 1369
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 4 cut(s) 62, 687, 1362, 1683
Zsp2I ATGCAT 1 cut(s) 1495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.