Rroxscaffold_4G00277240

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002642
Physical Location & Seq
Reverse (-)
679040 .. 680920
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277240.1

Sequence Viewer

Length: 1485 bp
ATGCTGACGCAGTCCATTTTGTGTCCCAAGTCACCTTCAGTGTCATCATCCTCAGGTAGCTTCTTCACTTCTCTCTCTCTGCTCTATCCAACTTGTCTCAATTACTCTGAGAAACACAAGCTGCATGCTTCTCTAACTCGCTCAAATCCCAGCGAGGGTGTCAAAGAATTCAACTTTCTAGCTTTAAACGACGAAACTCATGCAATTACTGACCACCCAAATGGCCCAACTCTCGGAGAGGAAATTTCTTTTGAGTTATACAACCATTTGTTACAAGATTTTTGTAGAGTTGGGCATGTTGATAAGGCCATGGCTCTTCTTGCTCGAATGGAAGCTCTTGGCTTTCGACCCAATTCGATATCCTACACTCGTTTAATTGATGCTCTGGGAAGTATTGGGAGGACTTTGGAGGCTGATGTGTTGTTTCAGGAAATGATATTTCTGGGGTTTAGGCCAAGAATTAAGCTTTACAATGTGTTGCTTAGAGGGTTTTTGAAGAAAGGTTTGTTAGGACTCGCAATTAGAGTTTTGGGGGTAATGGGTGATTTGGGTACTGAGAGAAATCAAGAAACTTATGAGATTTTACTTGATTACTATGTCAATGCTGGGAGGTTGGAGGATACTTGGTCAATGATTAATGAGATGAAGCGGAAGAGGTTTCGGCTGAGCTCGTTTGTGTACAGTAAGGTTATTGGTCTTTATAGGGACAATGGGATGTGGAAGAAAGCAATGGACATTGTGGAGGAGATAAAAGAAATGGGGATGGTATTAGACAAACAGATTTACAACAGCATTATTGACACATTTGGGAAATATGATGAGTTGGATGAAGCATTGGAAGTCTTTGGGAAAATGAAACAAGAAGGTGTAAGGCCTGATATTTCGACTTTTAATTCGTTGATACGGTGGCACTGTAAGTCTGGGTTGTTAAAGAAGGCCCTGGAGTTGTTTACCGAGATGCAAGAACAAGGATTATATCCTGATCCGAAAATGTTTGTTACTATTATCAGTAGATTGGGGGAGCAAGGGAAGTGGGATATGATACAGAAGACCTTTGAGAATATGAGAAGCCGAGGGCATAAAAAAAGTGGGACTATTTATGCTGCTCTGGTTGACATATATGGGCAATATGGAAAATTTCAGGACGCAGAAGAGTGTATATCTGCACTGAAGGCAGGAGGTCTTGTTCTCTCTGCCAGTATGTTTTGTGTCTTAGCAAATGCCTATGCCCAGCAGGGATTATGTGACCAGACAGTTAAAGTACTTCAGCTCATGGAAGCTGAGGGAATCGAAACCAAATGTGATAATGCTGAATCCCCCGATGTAGTTACTTATACTACCCTTATGAAGGCGTTTATTCGAGCAAGGAAGTATGATAAGGTCCCTGAGATATACATGGATATGGAACATGCTGGATGCACCCCAGATAGGAAGGCTAGACAGATGTTAGAAGTCGCTTCATTGGTCCTTCAACAGAGGGACTGA

Protein Analysis

494

Amino Acids

56.05

Weight (kDa)

6.36

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 87 - 129 4.6e-10 PPR repeat family
PPR_2 PF13041 154 - 197 9.8e-06 PPR repeat family
TPR_24 PF23276 167 - 284 3.3e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 190 - 231 4.9e-07 PPR repeat family
TPR_24 PF23276 236 - 348 6.1e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 246 - 302 1.8e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 261 - 306 3.4e-12 PPR repeat family
PPR PF01535 261 - 290 6.1e-06 PPR repeat
PPR_1 PF12854 289 - 321 8.1e-10 PPR repeat
PPR_2 PF13041 292 - 328 1.8e-10 PPR repeat family
PPR PF01535 295 - 325 1.5e-08 PPR repeat
PPR_2 PF13041 440 - 477 1.9e-08 PPR repeat family
PPR_3 PF13812 440 - 476 1.6e-06 Pentatricopeptide repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 649
AclWI GGATC 1 cut(s) 977
AcsI RAATTY 3 cut(s) 167, 243, 1136
AcuI CTGAAG 3 cut(s) 21, 1190, 1250
AfaI GTAC 3 cut(s) 553, 680, 1263
AfiI CCNNNNNNNGG 4 cut(s) 155, 233, 1348, 1428
AgsI TTSAA 3 cut(s) 172, 496, 1472
AjnI CCWGG 1 cut(s) 939
AloI GAACNNNNNNTCC 2 cut(s) 1170, 1202
AluBI AGCT 8 cut(s) 60, 121, 182, 335, 466, 669, 1270, 1280
AluI AGCT 8 cut(s) 60, 121, 182, 335, 466, 669, 1270, 1280
Alw21I GWGCWC 1 cut(s) 671
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 977
AoxI GGCC 5 cut(s) 223, 306, 452, 872, 936
ApeKI GCWGC 2 cut(s) 121, 1103
ApoI RAATTY 3 cut(s) 167, 243, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 877, 909
AseI ATTAAT 1 cut(s) 636
AspS9I GGNCC 4 cut(s) 224, 937, 1381, 1465
AsuHPI GGTGA 2 cut(s) 24, 554
AvaII GGWCC 2 cut(s) 1381, 1465
AxyI CCTNAGG 1 cut(s) 52
BanII GRGCYC 1 cut(s) 671
BbsI GAAGAC 1 cut(s) 1055
Bbv12I GWGCWC 1 cut(s) 671
BbvCI CCTCAGC 1 cut(s) 1281
BbvI GCAGC 2 cut(s) 108, 1090
BccI CCATC 1 cut(s) 757
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 1 cut(s) 941
BciVI GTATCC 1 cut(s) 613
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 2 cut(s) 179, 1437
BfuI GTATCC 1 cut(s) 613
BisI GCNGC 2 cut(s) 122, 1104
BlpI GCTNAGC 1 cut(s) 665
BlsI GCNGC 2 cut(s) 123, 1105
BmcAI AGTACT 1 cut(s) 1263
Bme1390I CCNGG 1 cut(s) 941
Bme18I GGWCC 2 cut(s) 1381, 1465
BmgT120I GGNCC 4 cut(s) 224, 937, 1381, 1465
BmiI GGNNCC 1 cut(s) 1383
BmrFI CCNGG 1 cut(s) 941
BmsI GCATC 3 cut(s) 370, 948, 1406
BpiI GAAGAC 1 cut(s) 1055
BpmI CTGGAG 1 cut(s) 962
Bpu10I CCTNAGC 1 cut(s) 1281
Bpu1102I GCTNAGC 1 cut(s) 665
BsaJI CCNNGG 3 cut(s) 309, 939, 1072
BsaXI ACNNNNNCTCC 2 cut(s) 1170, 1200
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 233, 1348, 1428
Bse1I ACTGG 1 cut(s) 1197
Bse21I CCTNAGG 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 1 cut(s) 941
BseDI CCNNGG 3 cut(s) 309, 939, 1072
BseGI GGATG 5 cut(s) 47, 720, 768, 832, 1421
BseLI CCNNNNNNNGG 4 cut(s) 155, 233, 1348, 1428
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 6 cut(s) 66, 99, 546, 656, 1272, 1377
BseNI ACTGG 1 cut(s) 1197
BseRI GAGGAG 1 cut(s) 758
BseXI GCAGC 2 cut(s) 108, 1090
BseYI CCCAGC 3 cut(s) 149, 605, 1230
BsgI GTGCAG 1 cut(s) 1149
BshFI GGCC 5 cut(s) 225, 308, 454, 874, 938
BsiHKAI GWGCWC 1 cut(s) 671
BslFI GGGAC 4 cut(s) 9, 719, 1105, 1367
BslI CCNNNNNNNGG 4 cut(s) 155, 233, 1348, 1428
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 4 cut(s) 9, 719, 1105, 1367
BsnI GGCC 5 cut(s) 225, 308, 454, 874, 938
Bsp1286I GDGCHC 1 cut(s) 671
Bsp1407I TGTACA 1 cut(s) 678
Bsp143I GATC 1 cut(s) 982
Bsp1720I GCTNAGC 1 cut(s) 665
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 1 cut(s) 649
BspANI GGCC 5 cut(s) 225, 308, 454, 874, 938
BspCNI CTCAG 6 cut(s) 65, 100, 547, 657, 1273, 1378
BspLI GGNNCC 1 cut(s) 1383
BspPI GGATC 1 cut(s) 977
BspQI GCTCTTC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 735
BsrGI TGTACA 1 cut(s) 678
BsrI ACTGG 1 cut(s) 1197
BssECI CCNNGG 3 cut(s) 309, 939, 1072
BssMI GATC 1 cut(s) 982
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 4 cut(s) 683, 906, 914, 1255
Bst6I CTCTTC 3 cut(s) 321, 647, 1146
BstAUI TGTACA 1 cut(s) 678
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 8 cut(s) 52, 108, 482, 555, 665, 1213, 1281, 1386
BstDSI CCRYGG 1 cut(s) 309
BstENI CCTNNNNNAGG 1 cut(s) 1346
BstF5I GGATG 5 cut(s) 47, 720, 768, 832, 1421
BstKTI GATC 1 cut(s) 985
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 1 cut(s) 982
BstMWI GCNNNNNNNGC 2 cut(s) 320, 1172
BstNI CCWGG 1 cut(s) 941
BstNSI RCATGY 3 cut(s) 128, 299, 1412
BstSCI CCNGG 1 cut(s) 939
BstV1I GCAGC 2 cut(s) 108, 1090
BstV2I GAAGAC 1 cut(s) 1055
BstXI CCANNNNNNTGG 1 cut(s) 221
Bsu36I CCTNAGG 1 cut(s) 52
BsuI GTATCC 1 cut(s) 613
BsuRI GGCC 5 cut(s) 225, 308, 454, 874, 938
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 5 cut(s) 47, 720, 768, 832, 1421
BtsIMutI CAGTG 3 cut(s) 45, 910, 1166
Cac8I GCNNGC 1 cut(s) 126
Cfr13I GGNCC 4 cut(s) 224, 937, 1381, 1465
CseI GACGC 2 cut(s) 16, 1154
Csp6I GTAC 3 cut(s) 552, 679, 1262
CspCI CAANNNNNGTGG 2 cut(s) 1013, 1048
CviAII CATG 7 cut(s) 125, 200, 296, 310, 1273, 1396, 1409
CviQI GTAC 3 cut(s) 552, 679, 1262
DdeI CTNAG 8 cut(s) 52, 108, 482, 555, 665, 1213, 1281, 1386
DpnI GATC 1 cut(s) 984
DpnII GATC 1 cut(s) 982
DraI TTTAAA 1 cut(s) 186
Eam1104I CTCTTC 3 cut(s) 321, 647, 1146
EarI CTCTTC 3 cut(s) 321, 647, 1146
Ecl136II GAGCTC 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 309
Eco147I AGGCCT 1 cut(s) 874
Eco24I GRGCYC 1 cut(s) 671
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 1381, 1465
Eco53kI GAGCTC 1 cut(s) 669
Eco57I CTGAAG 3 cut(s) 21, 1190, 1250
Eco81I CCTNAGG 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 669
EcoNI CCTNNNNNAGG 1 cut(s) 1346
EcoO109I RGGNCCY 2 cut(s) 937, 1381
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 939
EcoRV GATATC 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 671
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 7 cut(s) 128, 203, 299, 313, 1276, 1399, 1412
FaqI GGGAC 4 cut(s) 9, 719, 1105, 1367
FatI CATG 7 cut(s) 124, 199, 295, 309, 1272, 1395, 1408
Fnu4HI GCNGC 2 cut(s) 122, 1104
FokI GGATG 5 cut(s) 34, 727, 775, 839, 1428
FriOI GRGCYC 1 cut(s) 671
Fsp4HI GCNGC 2 cut(s) 122, 1104
FspBI CTAG 2 cut(s) 179, 1437
GluI GCNGC 2 cut(s) 122, 1104
GsaI CCCAGC 3 cut(s) 153, 609, 1234
GsuI CTGGAG 1 cut(s) 962
HaeIII GGCC 5 cut(s) 225, 308, 454, 874, 938
HgaI GACGC 2 cut(s) 16, 1154
Hin1II CATG 7 cut(s) 128, 203, 299, 313, 1276, 1399, 1412
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 3 cut(s) 513, 1287, 1313
HphI GGTGA 2 cut(s) 24, 554
Hpy166II GTNNAC 3 cut(s) 679, 951, 1114
Hpy188I TCNGA 3 cut(s) 109, 236, 987
Hpy188III TCNNGA 4 cut(s) 428, 566, 980, 1142
Hpy8I GTNNAC 3 cut(s) 679, 951, 1114
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 7 cut(s) 45, 857, 928, 1165, 1342, 1426, 1478
HpyCH4III ACNGT 4 cut(s) 683, 906, 914, 1255
HpyCH4V TGCA 5 cut(s) 124, 203, 961, 1166, 1419
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 1172
HpyF3I CTNAG 8 cut(s) 52, 108, 482, 555, 665, 1213, 1281, 1386
Hsp92II CATG 7 cut(s) 128, 203, 299, 313, 1276, 1399, 1412
Kzo9I GATC 1 cut(s) 982
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 1 cut(s) 1021
Lsp1109I GCAGC 2 cut(s) 108, 1090
LweI GCATC 3 cut(s) 370, 948, 1406
MaeI CTAG 2 cut(s) 179, 1437
MaeIII GTNAC 5 cut(s) 30, 270, 997, 1244, 1327
MalI GATC 1 cut(s) 984
MboI GATC 1 cut(s) 982
MboII GAAGA 7 cut(s) 55, 308, 508, 664, 733, 1060, 1163
MhlI GDGCHC 1 cut(s) 671
MlyI GAGTC 1 cut(s) 507
MmeI TCCRAC 3 cut(s) 113, 594, 804
MseI TTAA 7 cut(s) 185, 374, 462, 636, 891, 929, 1257
MslI CAYNNNNRTG 2 cut(s) 219, 1400
MspR9I CCNGG 1 cut(s) 941
MvaI CCWGG 1 cut(s) 941
MwoI GCNNNNNNNGC 2 cut(s) 320, 1172
NcoI CCATGG 1 cut(s) 309
NdeII GATC 1 cut(s) 982
NlaIII CATG 7 cut(s) 128, 203, 299, 313, 1276, 1399, 1412
NlaIV GGNNCC 1 cut(s) 1383
NmeAIII GCCGAG 1 cut(s) 1097
NmuCI GTSAC 2 cut(s) 30, 1244
NspI RCATGY 3 cut(s) 128, 299, 1412
PaeI GCATGC 1 cut(s) 128
PceI AGGCCT 1 cut(s) 874
PciSI GCTCTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 1287, 1313
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 2 cut(s) 123, 1105
PleI GAGTC 1 cut(s) 507
PpsI GAGTC 1 cut(s) 507
PpuMI RGGWCCY 1 cut(s) 1381
PshBI ATTAAT 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 1381
Psp6I CCWGG 1 cut(s) 939
PspFI CCCAGC 3 cut(s) 149, 605, 1230
PspGI CCWGG 1 cut(s) 939
PspN4I GGNNCC 1 cut(s) 1383
PspPI GGNCC 4 cut(s) 224, 937, 1381, 1465
PspPPI RGGWCCY 1 cut(s) 1381
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 3 cut(s) 553, 680, 1263
RsaNI GTAC 3 cut(s) 552, 679, 1262
RseI CAYNNNNRTG 2 cut(s) 219, 1400
SacI GAGCTC 1 cut(s) 671
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 7 cut(s) 185, 374, 462, 636, 891, 929, 1257
SatI GCNGC 2 cut(s) 122, 1104
Sau3AI GATC 1 cut(s) 982
Sau96I GGNCC 4 cut(s) 224, 937, 1381, 1465
ScaI AGTACT 1 cut(s) 1263
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 1 cut(s) 941
SduI GDGCHC 1 cut(s) 671
SfaNI GCATC 3 cut(s) 370, 948, 1406
SinI GGWCC 2 cut(s) 1381, 1465
SmiMI CAYNNNNRTG 2 cut(s) 219, 1400
SphI GCATGC 1 cut(s) 128
SseBI AGGCCT 1 cut(s) 874
SsiI CCGC 1 cut(s) 649
SspMI CTAG 2 cut(s) 179, 1437
SstI GAGCTC 1 cut(s) 671
StuI AGGCCT 1 cut(s) 874
StyD4I CCNGG 1 cut(s) 939
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 4 cut(s) 683, 906, 914, 1255
TaqI TCGA 6 cut(s) 325, 346, 356, 884, 1290, 1360
TatI WGTACW 2 cut(s) 678, 1261
TfiI GAWTC 2 cut(s) 1287, 1313
Tru1I TTAA 7 cut(s) 185, 374, 462, 636, 891, 929, 1257
Tru9I TTAA 7 cut(s) 185, 374, 462, 636, 891, 929, 1257
TscAI CASTG 3 cut(s) 45, 917, 1173
TseFI GTSAC 2 cut(s) 30, 1244
TseI GCWGC 2 cut(s) 121, 1103
Tsp45I GTSAC 2 cut(s) 30, 1244
TspDTI ATGAA 5 cut(s) 659, 843, 869, 1361, 1449
TspRI CASTG 3 cut(s) 45, 917, 1173
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 1381, 1465
VspI ATTAAT 1 cut(s) 636
XagI CCTNNNNNAGG 1 cut(s) 1346
XapI RAATTY 3 cut(s) 167, 243, 1136
XceI RCATGY 3 cut(s) 128, 299, 1412
XspI CTAG 2 cut(s) 179, 1437
ZrmI AGTACT 1 cut(s) 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.