RchiOBHm_Chr1g0383651

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002642
Physical Location & Seq
Forward (+)
67873224 .. 67875371
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60655

Sequence Viewer

Length: 1566 bp
ATGCTGACGCAGTCCATTTTGTGTCCCAAGTCACCTTCAGTGTCATCATCCTCAGGTAGCTTCTTCACTTCTCTCTCTCTGTTCTATCCAACTTGTCTCAATTACTCTGAGAAACACAAGCTGCATGCTTCTCTAACTCGCTCAAATCCGAGCGAGGGTGTCAAAGAATTCAACTTTCTAGCTTCAAACGACGAAACTCATCCAGTTACTGACTACCCAAATGTCCCAACTCTCGGAGAGGAAATTTCCTTTGAGTTATACAACCATTTGTTACAAGATTTTTGTAGAGTTGGGCATGTTGATAAGGCCATGGCTCTTCTTGCTCGAATGGAAGCTCTTGGCTTTCGACCCAATTCGATATCCTACACTCGTTTAATTGATGCTCTGGGAAGTATTGGGAGGACTTTGGAGGCTGATGTGTTGTTTCAGGAAATGATATTTGTGGGGTTTAGGCCAAGAATTAAGCTTTACAATGTGTTGCTTAGAGGGTTTTTGAAGAAAGGTTTGTTAGGACTCGCAATTAGAGTTTTGGGGGTAATGGGTGATTTGGGTACTGAGAGAAATCAAGAAACTTATGAGATTTTACTTGATTACTATGTCAGTGCTGGGAGGTTGGAGGATACTTGGTCAATGATTAATGAGATGAAGCAGAAAAGGTTTCGGCTGAGCTCGTTTGTGTACAGTAAGGTTATTGGTCTTTATAGGGACAATGGGATGTGGAAGAAAGCAATCGACATTGTGGAAGAGATAAGAGAAATGGGGATGGCATTAGACAAACAGATTTACAACAGCATTATTGATACATTTGGGAAATATGGTGAGTTGGATGAAGCATTGGAAGTCTTTGGGAAAATGAAACAAGAAGGTGTAAGGCCTGATATTTCGACCTTTAATTCGTTGATACGGTGGCACTGCAAGTCTGGTTTGTTAAAGAAGGCCCTTGAGTTGTTTACCGAGATGCAAGAACAAGGATTATATCCTGATCCGAAAATGTTTGTTACTATTATCAGTAGATTGGGGGAGCAAGGGAAGTGGGATATGATACAGAAGACCTTTGAGAATATGAGAAGCCAAGGGCATAAAAAAAGTGGGACTATTTATGCTGCTCTGGTTGATATTTATGGGCAATATGGAAAATTTCAGGACGCAGAAGAGTGTATATCTGCACTAAAGGCAGAAGGTCTTGTTCTCTCTGCCAGTATGTTTTGTGTCTTAGCAAATGCCTATGCCCAGCAGGGATTATGTGACCAGACAGTTAAAGTACTTCAGCTCATGGAAGCTGAGGGAATTGAACCAAATGTGATCATGCTGAATGTTTTGATTAATGCATTTGGTATCGCTGGTAGACATTTGGAGGCTTTGTCCATTTATCACCATATAAAAGAAAGTGGTTTAAGCCCCGATGTAGTTACTTATACTACCCTTATGAAGGCGTTTATTCGAGCAAGGAAGTATGATAAGGTCCCTGAGATATACATGGATATGGAACATGCTGGATGCACCCCAGATAGGAAGGCTAGACAGATGTTAGAAGTCGCTTCATTGGTCCTTCAACAGAGGGATTGA

Protein Analysis

521

Amino Acids

58.99

Weight (kDa)

6.31

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 87 - 129 4.9e-10 PPR repeat family
TPR_24 PF23276 167 - 284 1e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 188 - 231 8.1e-07 PPR repeat family
TPR_24 PF23276 236 - 301 4e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 242 - 375 9.5e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 246 - 302 2.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 254 - 286 5.3e-06 PPR repeat
PPR PF01535 261 - 290 5.4e-07 PPR repeat
PPR_2 PF13041 261 - 302 3e-11 PPR repeat family
PPR_1 PF12854 289 - 321 8.6e-10 PPR repeat
PPR_2 PF13041 292 - 336 8e-11 PPR repeat family
PPR PF01535 295 - 325 1.6e-08 PPR repeat
TPR_24 PF23276 336 - 459 1.9e-07 Fungal tetratrico peptide repeats
TPR_24 PF23276 374 - 492 9e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 390 - 445 8.8e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 432 - 480 1.3e-11 PPR repeat family
PPR_3 PF13812 455 - 503 5.9e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 467 - 504 2.1e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1345
AclWI GGATC 1 cut(s) 977
AcsI RAATTY 3 cut(s) 167, 243, 1136
AcuI CTGAAG 2 cut(s) 21, 1250
AfaI GTAC 3 cut(s) 553, 680, 1263
AfiI CCNNNNNNNGG 4 cut(s) 155, 233, 1429, 1509
AgsI TTSAA 5 cut(s) 172, 186, 496, 1292, 1553
AluBI AGCT 8 cut(s) 60, 121, 182, 335, 466, 669, 1270, 1280
AluI AGCT 8 cut(s) 60, 121, 182, 335, 466, 669, 1270, 1280
Alw21I GWGCWC 1 cut(s) 671
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 977
AlwNI CAGNNNCTG 1 cut(s) 209
AoxI GGCC 4 cut(s) 306, 452, 872, 936
ApeKI GCWGC 2 cut(s) 121, 1103
ApoI RAATTY 3 cut(s) 167, 243, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 877, 909
AseI ATTAAT 2 cut(s) 636, 1323
AspS9I GGNCC 3 cut(s) 937, 1462, 1546
AsuHPI GGTGA 4 cut(s) 24, 554, 830, 1364
AvaII GGWCC 2 cut(s) 1462, 1546
AxyI CCTNAGG 1 cut(s) 52
BanII GRGCYC 1 cut(s) 671
BbsI GAAGAC 1 cut(s) 1055
Bbv12I GWGCWC 1 cut(s) 671
BbvCI CCTCAGC 1 cut(s) 1281
BbvI GCAGC 2 cut(s) 108, 1090
BccI CCATC 1 cut(s) 757
BciVI GTATCC 1 cut(s) 613
BclI TGATCA 1 cut(s) 1302
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 2 cut(s) 179, 1518
BfuI GTATCC 1 cut(s) 613
BisI GCNGC 2 cut(s) 122, 1104
BlpI GCTNAGC 1 cut(s) 665
BlsI GCNGC 2 cut(s) 123, 1105
BmcAI AGTACT 1 cut(s) 1263
Bme18I GGWCC 2 cut(s) 1462, 1546
BmgT120I GGNCC 3 cut(s) 937, 1462, 1546
BmiI GGNNCC 1 cut(s) 1464
BmsI GCATC 3 cut(s) 370, 948, 1487
BpiI GAAGAC 1 cut(s) 1055
Bpu10I CCTNAGC 1 cut(s) 1281
Bpu1102I GCTNAGC 1 cut(s) 665
BpuEI CTTGAG 1 cut(s) 962
BsaJI CCNNGG 2 cut(s) 309, 1072
BsaXI ACNNNNNCTCC 2 cut(s) 1346, 1376
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 233, 1429, 1509
Bse1I ACTGG 2 cut(s) 203, 1197
Bse21I CCTNAGG 1 cut(s) 52
BseDI CCNNGG 2 cut(s) 309, 1072
BseGI GGATG 6 cut(s) 47, 199, 720, 768, 832, 1502
BseLI CCNNNNNNNGG 4 cut(s) 155, 233, 1429, 1509
BseMII CTCAG 6 cut(s) 66, 99, 546, 656, 1272, 1458
BseNI ACTGG 2 cut(s) 203, 1197
BseXI GCAGC 2 cut(s) 108, 1090
BseYI CCCAGC 2 cut(s) 605, 1230
BsgI GTGCAG 1 cut(s) 1149
BshFI GGCC 4 cut(s) 308, 454, 874, 938
BsiHKAI GWGCWC 1 cut(s) 671
BslFI GGGAC 5 cut(s) 9, 209, 719, 1105, 1448
BslI CCNNNNNNNGG 4 cut(s) 155, 233, 1429, 1509
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 5 cut(s) 9, 209, 719, 1105, 1448
BsnI GGCC 4 cut(s) 308, 454, 874, 938
Bsp1286I GDGCHC 1 cut(s) 671
Bsp1407I TGTACA 1 cut(s) 678
Bsp143I GATC 2 cut(s) 982, 1302
Bsp1720I GCTNAGC 1 cut(s) 665
Bsp19I CCATGG 1 cut(s) 309
BspANI GGCC 4 cut(s) 308, 454, 874, 938
BspCNI CTCAG 6 cut(s) 65, 100, 547, 657, 1273, 1459
BspLI GGNNCC 1 cut(s) 1464
BspPI GGATC 1 cut(s) 977
BspQI GCTCTTC 1 cut(s) 321
BsrGI TGTACA 1 cut(s) 678
BsrI ACTGG 2 cut(s) 203, 1197
BssECI CCNNGG 2 cut(s) 309, 1072
BssMI GATC 2 cut(s) 982, 1302
BssT1I CCWWGG 2 cut(s) 309, 1072
Bst4CI ACNGT 3 cut(s) 683, 906, 1255
Bst6I CTCTTC 3 cut(s) 321, 738, 1146
BstAUI TGTACA 1 cut(s) 678
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 8 cut(s) 52, 108, 482, 555, 665, 1213, 1281, 1467
BstDSI CCRYGG 1 cut(s) 309
BstENI CCTNNNNNAGG 1 cut(s) 1427
BstF5I GGATG 6 cut(s) 47, 199, 720, 768, 832, 1502
BstKTI GATC 2 cut(s) 985, 1305
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 982, 1302
BstMWI GCNNNNNNNGC 2 cut(s) 320, 1172
BstNSI RCATGY 3 cut(s) 128, 299, 1493
BstV1I GCAGC 2 cut(s) 108, 1090
BstV2I GAAGAC 1 cut(s) 1055
Bsu36I CCTNAGG 1 cut(s) 52
BsuI GTATCC 1 cut(s) 613
BsuRI GGCC 4 cut(s) 308, 454, 874, 938
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 6 cut(s) 47, 199, 720, 768, 832, 1502
BtsI GCAGTG 1 cut(s) 910
BtsIMutI CAGTG 3 cut(s) 45, 607, 910
Cac8I GCNNGC 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 209
Cfr13I GGNCC 3 cut(s) 937, 1462, 1546
CseI GACGC 2 cut(s) 16, 1154
Csp6I GTAC 3 cut(s) 552, 679, 1262
CspCI CAANNNNNGTGG 2 cut(s) 1013, 1048
CviAII CATG 7 cut(s) 125, 296, 310, 1273, 1306, 1477, 1490
CviQI GTAC 3 cut(s) 552, 679, 1262
DdeI CTNAG 8 cut(s) 52, 108, 482, 555, 665, 1213, 1281, 1467
DpnI GATC 2 cut(s) 984, 1304
DpnII GATC 2 cut(s) 982, 1302
Eam1104I CTCTTC 3 cut(s) 321, 738, 1146
EarI CTCTTC 3 cut(s) 321, 738, 1146
Ecl136II GAGCTC 1 cut(s) 669
Eco130I CCWWGG 2 cut(s) 309, 1072
Eco147I AGGCCT 1 cut(s) 874
Eco24I GRGCYC 1 cut(s) 671
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 1462, 1546
Eco53kI GAGCTC 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 21, 1250
Eco81I CCTNAGG 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 669
EcoNI CCTNNNNNAGG 1 cut(s) 1427
EcoO109I RGGNCCY 2 cut(s) 937, 1462
EcoRI GAATTC 1 cut(s) 167
EcoRV GATATC 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 309, 1072
EcoT22I ATGCAT 1 cut(s) 1330
EcoT38I GRGCYC 1 cut(s) 671
ErhI CCWWGG 2 cut(s) 309, 1072
FaeI CATG 7 cut(s) 128, 299, 313, 1276, 1309, 1480, 1493
FaqI GGGAC 5 cut(s) 9, 209, 719, 1105, 1448
FatI CATG 7 cut(s) 124, 295, 309, 1272, 1305, 1476, 1489
FbaI TGATCA 1 cut(s) 1302
FblI GTMKAC 1 cut(s) 1345
Fnu4HI GCNGC 2 cut(s) 122, 1104
FokI GGATG 6 cut(s) 34, 186, 727, 775, 839, 1509
FriOI GRGCYC 1 cut(s) 671
Fsp4HI GCNGC 2 cut(s) 122, 1104
FspBI CTAG 2 cut(s) 179, 1518
GluI GCNGC 2 cut(s) 122, 1104
GsaI CCCAGC 2 cut(s) 609, 1234
HaeIII GGCC 4 cut(s) 308, 454, 874, 938
HgaI GACGC 2 cut(s) 16, 1154
Hin1II CATG 7 cut(s) 128, 299, 313, 1276, 1309, 1480, 1493
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 1 cut(s) 513
HphI GGTGA 4 cut(s) 24, 554, 830, 1364
Hpy166II GTNNAC 3 cut(s) 679, 951, 1346
Hpy188I TCNGA 4 cut(s) 109, 150, 236, 987
Hpy188III TCNNGA 4 cut(s) 428, 566, 980, 1142
Hpy8I GTNNAC 3 cut(s) 679, 951, 1346
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 7 cut(s) 45, 857, 928, 1172, 1423, 1507, 1559
HpyCH4III ACNGT 3 cut(s) 683, 906, 1255
HpyCH4V TGCA 6 cut(s) 124, 915, 961, 1166, 1328, 1500
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 1172
HpyF3I CTNAG 8 cut(s) 52, 108, 482, 555, 665, 1213, 1281, 1467
Hsp92II CATG 7 cut(s) 128, 299, 313, 1276, 1309, 1480, 1493
Ksp22I TGATCA 1 cut(s) 1302
Kzo9I GATC 2 cut(s) 982, 1302
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 1 cut(s) 1021
Lsp1109I GCAGC 2 cut(s) 108, 1090
LweI GCATC 3 cut(s) 370, 948, 1487
MaeI CTAG 2 cut(s) 179, 1518
MaeIII GTNAC 6 cut(s) 30, 205, 270, 997, 1244, 1408
MalI GATC 2 cut(s) 984, 1304
MboI GATC 2 cut(s) 982, 1302
MboII GAAGA 7 cut(s) 55, 308, 508, 733, 755, 1060, 1163
MhlI GDGCHC 1 cut(s) 671
MlyI GAGTC 1 cut(s) 507
MmeI TCCRAC 3 cut(s) 113, 594, 804
Mph1103I ATGCAT 1 cut(s) 1330
MseI TTAA 8 cut(s) 374, 462, 636, 891, 929, 1257, 1323, 1394
MslI CAYNNNNRTG 1 cut(s) 1481
MwoI GCNNNNNNNGC 2 cut(s) 320, 1172
NcoI CCATGG 1 cut(s) 309
NdeII GATC 2 cut(s) 982, 1302
NlaIII CATG 7 cut(s) 128, 299, 313, 1276, 1309, 1480, 1493
NlaIV GGNNCC 1 cut(s) 1464
NmuCI GTSAC 2 cut(s) 30, 1244
NsiI ATGCAT 1 cut(s) 1330
NspI RCATGY 3 cut(s) 128, 299, 1493
PaeI GCATGC 1 cut(s) 128
PceI AGGCCT 1 cut(s) 874
PciSI GCTCTTC 1 cut(s) 321
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 2 cut(s) 123, 1105
PleI GAGTC 1 cut(s) 507
PpsI GAGTC 1 cut(s) 507
PpuMI RGGWCCY 1 cut(s) 1462
PshBI ATTAAT 2 cut(s) 636, 1323
Psp124BI GAGCTC 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 1462
PspFI CCCAGC 2 cut(s) 605, 1230
PspN4I GGNNCC 1 cut(s) 1464
PspPI GGNCC 3 cut(s) 937, 1462, 1546
PspPPI RGGWCCY 1 cut(s) 1462
PstNI CAGNNNCTG 1 cut(s) 209
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 3 cut(s) 553, 680, 1263
RsaNI GTAC 3 cut(s) 552, 679, 1262
RseI CAYNNNNRTG 1 cut(s) 1481
SacI GAGCTC 1 cut(s) 671
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 8 cut(s) 374, 462, 636, 891, 929, 1257, 1323, 1394
SatI GCNGC 2 cut(s) 122, 1104
Sau3AI GATC 2 cut(s) 982, 1302
Sau96I GGNCC 3 cut(s) 937, 1462, 1546
ScaI AGTACT 1 cut(s) 1263
SchI GAGTC 1 cut(s) 507
SduI GDGCHC 1 cut(s) 671
SfaNI GCATC 3 cut(s) 370, 948, 1487
SinI GGWCC 2 cut(s) 1462, 1546
SmiMI CAYNNNNRTG 1 cut(s) 1481
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
SphI GCATGC 1 cut(s) 128
SseBI AGGCCT 1 cut(s) 874
SspMI CTAG 2 cut(s) 179, 1518
SstI GAGCTC 1 cut(s) 671
StuI AGGCCT 1 cut(s) 874
StyI CCWWGG 2 cut(s) 309, 1072
TaaI ACNGT 3 cut(s) 683, 906, 1255
TaqI TCGA 6 cut(s) 325, 346, 356, 732, 884, 1441
TatI WGTACW 2 cut(s) 678, 1261
Tru1I TTAA 8 cut(s) 374, 462, 636, 891, 929, 1257, 1323, 1394
Tru9I TTAA 8 cut(s) 374, 462, 636, 891, 929, 1257, 1323, 1394
TscAI CASTG 3 cut(s) 45, 607, 917
TseFI GTSAC 2 cut(s) 30, 1244
TseI GCWGC 2 cut(s) 121, 1103
Tsp45I GTSAC 2 cut(s) 30, 1244
TspDTI ATGAA 5 cut(s) 659, 843, 869, 1442, 1530
TspRI CASTG 3 cut(s) 45, 607, 917
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 1462, 1546
VspI ATTAAT 2 cut(s) 636, 1323
XagI CCTNNNNNAGG 1 cut(s) 1427
XapI RAATTY 3 cut(s) 167, 243, 1136
XceI RCATGY 3 cut(s) 128, 299, 1493
XmiI GTMKAC 1 cut(s) 1345
XspI CTAG 2 cut(s) 179, 1518
ZrmI AGTACT 1 cut(s) 1263
Zsp2I ATGCAT 1 cut(s) 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.