Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0002642

Physical Location & Seq

Genomic Coordinates Forward (+)

54390270 .. 54392068

Gene Structure 1799 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG425300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGCTGACGCAGTCCATTTTGTGTCCCAAGTCACCTTCAGTGTCATCGTCCTCAGGTAGCTTCTTCACTTCTCTCTCTCTGTTCTGTCCAACTTGTCTCAAATACTCTGAGAAACACAAGCTGCATGCTTCTCTAACTCGCTCAAATCCCAGCGAGGGTGTCAAAGAATTCAACTTTCTAGCTTCAAACGACGAAACTCATCCAGTTACTGACTACCCAAATGTCCCAACTCTCGGAGAGGAAATTTCCTTTGAGTTATACAACCATTTGTTACAAGATTTTTGTAGAGTTGGGCATGTTGATAAGGCCATGGCGCTTCTTGCTCAAATGGAAGCTCTTGGCTTTCGACCCAATTCGGTATCCTACACTCGTTTAATTGATGCTCTGGGAAGTATTGGGAGGACTTTGGAGGCTGATGTGTTGTTTCAGGAAATGATATTTGTGGGGTTTAGGCCAAGAATTAAGCTTTACAATGTGTTGCTTAGAGGGTTTTTGAAGAAAGGTTTGTTAGGACTCGCAATTAGAGTTTTGGGGGTAATGGGTGATTTGGGTACTGAGAGAAATCAAGAAACTTATGAGATTTTACTTGATTACTATGTCAATGCTGGGAGGTTGGAGGATACTTGGTCAATGATTAATGAGATGAAGCGGAAGAGGTTTCGGCTGAGCTCGTTTGTGTACAGTAAGGTTATTGGTCTTTATAGGGACAATGGGATGTGGAAGAAAGCAATCGACATTGTGGAAGAGATAAGAGAAATGGGGATGGCATTAGACAAACAGATTTACAACAGCATTATTGATACATTTGGGAAATATGGTGAGTTGGATGAAGCATTGGAAGTCTTTGGGAAAATGAAACAAGAAGGTGTAAGGCCTGATATTTCGACCTTTAATTCGTTGATACGGTGGCACTGCAAGTCTGGTTTGTTAAAGAAGGCCCTTGAGTTGTTTACCGAGATGCAAGAACAAGGATTATATCCTGATCCGAAAATGTTTGTTACTATTATCAGTAGATTGGGGGAGCAAGGGAAGTGGGATATGATACAGAAGACCTTTGAGAATATGAGAAGCCGAGGGCATAAAAAAAGTGGGACTATTTATGCTGCTCTGGTTGACATTTATGGGCAATATGGAAAATTTCAGGACGCAGAAGAGTGTATATCTGCACTAAAGGCAGAAGGTCTTGTTCCCTCTGCCAGTATGTTTTGTGTCTTAGCAAATGCCTATGCCCAGCAGGGATTATGTGATCAGACAGTCAAAGTACTTCAGCTCATGGAAGCCGAGGGAATCGAACCAAATGTGATAATGCTGAATGTTTTGATTAATGCATTTGGTATTGCTGGTAGACATTTGGAGGCTTTGTCCATTTATCACCATATAAAAGAAAGTGGTTTAAGCCCCGATGTAGTTACTTATACTACCCTTATGAAGGCGTTTATTCGAGCAAGGAAGTATGATAAGGTCCCTGAGATATACATGGATATGGAACATGCTGGATGCACCCCAGATAGAAAGGCTAGACAGATGTTAGAGGTCGCTTCATTGGTCCTTCAACAGAGGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0009685 GO:0009987 GO:0010817 GO:0016101 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0042445 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0045543 GO:0051213 GO:0052635 GO:0055114 GO:0065007 GO:0065008 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

521

Amino Acids

58.97

Weight (kDa)

6.84

Isoelectric Point (pI)

37.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	87 - 129	8.9e-11	PPR repeat family
TPR_24	PF23276	167 - 282	2.3e-09	Fungal tetratrico peptide repeats
PPR_2	PF13041	188 - 231	3e-07	PPR repeat family
PPR_long	PF17177	212 - 328	9.7e-06	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	236 - 336	2.2e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	246 - 302	2.1e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	254 - 286	5.3e-06	PPR repeat
PPR	PF01535	261 - 290	5.4e-07	PPR repeat
PPR_2	PF13041	261 - 306	6.3e-13	PPR repeat family
PPR_1	PF12854	289 - 321	8.6e-10	PPR repeat
PPR_2	PF13041	292 - 328	2e-10	PPR repeat family
PPR	PF01535	295 - 325	1.6e-08	PPR repeat
TPR_24	PF23276	336 - 459	1.7e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	390 - 445	8.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	432 - 480	1.3e-11	PPR repeat family
PPR_3	PF13812	455 - 503	5.9e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	467 - 504	2.1e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0002642)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G42560 AT5G36300 AT5G36300
fragaria_vesca	FvH4_7g33490 FvH4_7g33490 FvH4_7g33530 FvH4_7g33530 FvH4_7g33530 FvH4_7g33530 FvH4_7g33530 FvH4_7g33530 FvH4_7g33530 FvH4_7g33530
malus_domestica	MD01G1232900.v1.1
prunus_persica	Prupe.2G323300_v2.0.a1
pyrus_communis	pycom01g24130 pycom07g27830
rosa_chinensis	RchiOBHm_Chr1g0383591 RchiOBHm_Chr1g0383651
rosa_laevigata	RLG00000026099
rosa_multiflora	Rmu_sc0012471.1_g000010 Rmu_sc0012471.1_g000013
rosa_roxburghii	Rroxscaffold_2G00116820 Rroxscaffold_2G00117690 Rroxscaffold_3G00229630 Rroxscaffold_4G00277240 Rroxscaffold_4G00302110 Rroxscaffold_5G00350700 Rroxscaffold_5G00350710 Rroxscaffold_7G00181950
rosa_rugosa	Rorug01G0442100 Rorug01G0442200
rosa_samantha	Rh1AG467600 Rh1AG467800 Rh1BG425300 Rh1CG437900 Rh1CG438100 Rh1DG458100
rosa_wichuraiana	Rw1G040460 Rw1G040480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1345
AciI	CCGC	1 cut(s)	649
AclWI	GGATC	1 cut(s)	977
AcsI	RAATTY	3 cut(s)	167, 243, 1136
AcuI	CTGAAG	2 cut(s)	21, 1250
AfaI	GTAC	3 cut(s)	553, 680, 1263
AfiI	CCNNNNNNNGG	3 cut(s)	155, 233, 1429
AgsI	TTSAA	4 cut(s)	172, 186, 496, 1553
AluBI	AGCT	7 cut(s)	60, 121, 182, 335, 466, 669, 1270
AluI	AGCT	7 cut(s)	60, 121, 182, 335, 466, 669, 1270
Alw21I	GWGCWC	1 cut(s)	671
Alw26I	GTCTC	1 cut(s)	101
AlwI	GGATC	1 cut(s)	977
AlwNI	CAGNNNCTG	1 cut(s)	209
AoxI	GGCC	4 cut(s)	306, 452, 872, 936
ApeKI	GCWGC	2 cut(s)	121, 1103
ApoI	RAATTY	3 cut(s)	167, 243, 1136
ArsI	GACNNNNNNTTYG	2 cut(s)	877, 909
AseI	ATTAAT	2 cut(s)	636, 1323
AspLEI	GCGC	1 cut(s)	316
AspS9I	GGNCC	3 cut(s)	937, 1462, 1546
AsuHPI	GGTGA	4 cut(s)	24, 554, 830, 1364
AvaII	GGWCC	2 cut(s)	1462, 1546
AxyI	CCTNAGG	1 cut(s)	52
BanII	GRGCYC	1 cut(s)	671
BbsI	GAAGAC	1 cut(s)	1055
Bbv12I	GWGCWC	1 cut(s)	671
BbvI	GCAGC	2 cut(s)	108, 1090
BccI	CCATC	1 cut(s)	757
BciVI	GTATCC	2 cut(s)	370, 613
BclI	TGATCA	1 cut(s)	1246
BcoDI	GTCTC	1 cut(s)	101
BfaI	CTAG	2 cut(s)	179, 1518
BfoI	RGCGCY	1 cut(s)	317
BfuI	GTATCC	2 cut(s)	370, 613
BisI	GCNGC	2 cut(s)	122, 1104
BlpI	GCTNAGC	1 cut(s)	665
BlsI	GCNGC	2 cut(s)	123, 1105
BmcAI	AGTACT	1 cut(s)	1263
Bme18I	GGWCC	2 cut(s)	1462, 1546
BmgT120I	GGNCC	3 cut(s)	937, 1462, 1546
BmiI	GGNNCC	1 cut(s)	1464
BmsI	GCATC	3 cut(s)	370, 948, 1487
BpiI	GAAGAC	1 cut(s)	1055
Bpu1102I	GCTNAGC	1 cut(s)	665
BpuEI	CTTGAG	1 cut(s)	962
BsaJI	CCNNGG	3 cut(s)	309, 1072, 1281
BsaXI	ACNNNNNCTCC	2 cut(s)	1346, 1376
Bsc4I	CCNNNNNNNGG	3 cut(s)	155, 233, 1429
Bse1I	ACTGG	2 cut(s)	203, 1197
Bse21I	CCTNAGG	1 cut(s)	52
BseDI	CCNNGG	3 cut(s)	309, 1072, 1281
BseGI	GGATG	5 cut(s)	199, 720, 768, 832, 1502
BseLI	CCNNNNNNNGG	3 cut(s)	155, 233, 1429
BseMII	CTCAG	5 cut(s)	66, 99, 546, 656, 1458
BseNI	ACTGG	2 cut(s)	203, 1197
BseXI	GCAGC	2 cut(s)	108, 1090
BseYI	CCCAGC	3 cut(s)	149, 605, 1230
BsgI	GTGCAG	1 cut(s)	1149
BshFI	GGCC	4 cut(s)	308, 454, 874, 938
BsiHKAI	GWGCWC	1 cut(s)	671
BslFI	GGGAC	5 cut(s)	9, 209, 719, 1105, 1448
BslI	CCNNNNNNNGG	3 cut(s)	155, 233, 1429
BsmAI	GTCTC	1 cut(s)	101
BsmFI	GGGAC	5 cut(s)	9, 209, 719, 1105, 1448
BsnI	GGCC	4 cut(s)	308, 454, 874, 938
Bsp1286I	GDGCHC	1 cut(s)	671
Bsp1407I	TGTACA	1 cut(s)	678
Bsp143I	GATC	2 cut(s)	982, 1246
Bsp1720I	GCTNAGC	1 cut(s)	665
Bsp19I	CCATGG	1 cut(s)	309
BspACI	CCGC	1 cut(s)	649
BspANI	GGCC	4 cut(s)	308, 454, 874, 938
BspCNI	CTCAG	5 cut(s)	65, 100, 547, 657, 1459
BspLI	GGNNCC	1 cut(s)	1464
BspPI	GGATC	1 cut(s)	977
BsrGI	TGTACA	1 cut(s)	678
BsrI	ACTGG	2 cut(s)	203, 1197
BssECI	CCNNGG	3 cut(s)	309, 1072, 1281
BssMI	GATC	2 cut(s)	982, 1246
BssT1I	CCWWGG	1 cut(s)	309
Bst4CI	ACNGT	3 cut(s)	683, 906, 1255
Bst6I	CTCTTC	3 cut(s)	647, 738, 1146
BstAUI	TGTACA	1 cut(s)	678
BstC8I	GCNNGC	1 cut(s)	126
BstDEI	CTNAG	7 cut(s)	52, 108, 482, 555, 665, 1213, 1467
BstDSI	CCRYGG	1 cut(s)	309
BstENI	CCTNNNNNAGG	1 cut(s)	1427
BstF5I	GGATG	5 cut(s)	199, 720, 768, 832, 1502
BstH2I	RGCGCY	1 cut(s)	317
BstHHI	GCGC	1 cut(s)	316
BstKTI	GATC	2 cut(s)	985, 1249
BstMAI	GTCTC	1 cut(s)	101
BstMBI	GATC	2 cut(s)	982, 1246
BstMWI	GCNNNNNNNGC	2 cut(s)	320, 1172
BstNSI	RCATGY	3 cut(s)	128, 299, 1493
BstV1I	GCAGC	2 cut(s)	108, 1090
BstV2I	GAAGAC	1 cut(s)	1055
Bsu36I	CCTNAGG	1 cut(s)	52
BsuI	GTATCC	2 cut(s)	370, 613
BsuRI	GGCC	4 cut(s)	308, 454, 874, 938
BtgI	CCRYGG	1 cut(s)	309
BtsCI	GGATG	5 cut(s)	199, 720, 768, 832, 1502
BtsI	GCAGTG	1 cut(s)	910
BtsIMutI	CAGTG	2 cut(s)	45, 910
Cac8I	GCNNGC	1 cut(s)	126
CaiI	CAGNNNCTG	1 cut(s)	209
CfoI	GCGC	1 cut(s)	316
Cfr13I	GGNCC	3 cut(s)	937, 1462, 1546
CseI	GACGC	2 cut(s)	16, 1154
Csp6I	GTAC	3 cut(s)	552, 679, 1262
CspCI	CAANNNNNGTGG	2 cut(s)	1013, 1048
CviAII	CATG	6 cut(s)	125, 296, 310, 1273, 1477, 1490
CviQI	GTAC	3 cut(s)	552, 679, 1262
DdeI	CTNAG	7 cut(s)	52, 108, 482, 555, 665, 1213, 1467
DpnI	GATC	2 cut(s)	984, 1248
DpnII	GATC	2 cut(s)	982, 1246
Eam1104I	CTCTTC	3 cut(s)	647, 738, 1146
EarI	CTCTTC	3 cut(s)	647, 738, 1146
Ecl136II	GAGCTC	1 cut(s)	669
Eco130I	CCWWGG	1 cut(s)	309
Eco147I	AGGCCT	1 cut(s)	874
Eco24I	GRGCYC	1 cut(s)	671
Eco47I	GGWCC	2 cut(s)	1462, 1546
Eco53kI	GAGCTC	1 cut(s)	669
Eco57I	CTGAAG	2 cut(s)	21, 1250
Eco81I	CCTNAGG	1 cut(s)	52
EcoICRI	GAGCTC	1 cut(s)	669
EcoNI	CCTNNNNNAGG	1 cut(s)	1427
EcoO109I	RGGNCCY	2 cut(s)	937, 1462
EcoRI	GAATTC	1 cut(s)	167
EcoT14I	CCWWGG	1 cut(s)	309
EcoT22I	ATGCAT	1 cut(s)	1330
EcoT38I	GRGCYC	1 cut(s)	671
ErhI	CCWWGG	1 cut(s)	309
FaeI	CATG	6 cut(s)	128, 299, 313, 1276, 1480, 1493
FaqI	GGGAC	5 cut(s)	9, 209, 719, 1105, 1448
FatI	CATG	6 cut(s)	124, 295, 309, 1272, 1476, 1489
FbaI	TGATCA	1 cut(s)	1246
FblI	GTMKAC	1 cut(s)	1345
Fnu4HI	GCNGC	2 cut(s)	122, 1104
FokI	GGATG	5 cut(s)	186, 727, 775, 839, 1509
FriOI	GRGCYC	1 cut(s)	671
Fsp4HI	GCNGC	2 cut(s)	122, 1104
FspBI	CTAG	2 cut(s)	179, 1518
GlaI	GCGC	1 cut(s)	315
GluI	GCNGC	2 cut(s)	122, 1104
GsaI	CCCAGC	3 cut(s)	153, 609, 1234
HaeII	RGCGCY	1 cut(s)	317
HaeIII	GGCC	4 cut(s)	308, 454, 874, 938
HgaI	GACGC	2 cut(s)	16, 1154
HhaI	GCGC	1 cut(s)	316
Hin1II	CATG	6 cut(s)	128, 299, 313, 1276, 1480, 1493
Hin6I	GCGC	1 cut(s)	314
HinP1I	GCGC	1 cut(s)	314
HincII	GTYRAC	1 cut(s)	1114
HindII	GTYRAC	1 cut(s)	1114
HindIII	AAGCTT	1 cut(s)	464
HinfI	GANTC	2 cut(s)	513, 1287
HphI	GGTGA	4 cut(s)	24, 554, 830, 1364
Hpy166II	GTNNAC	4 cut(s)	679, 951, 1114, 1346
Hpy188I	TCNGA	4 cut(s)	109, 236, 987, 1251
Hpy188III	TCNNGA	4 cut(s)	428, 566, 980, 1142
Hpy8I	GTNNAC	4 cut(s)	679, 951, 1114, 1346
Hpy99I	CGWCG	1 cut(s)	194
HpyAV	CCTTC	6 cut(s)	45, 857, 928, 1172, 1423, 1559
HpyCH4III	ACNGT	3 cut(s)	683, 906, 1255
HpyCH4V	TGCA	6 cut(s)	124, 915, 961, 1166, 1328, 1500
HpyF10VI	GCNNNNNNNGC	2 cut(s)	320, 1172
HpyF3I	CTNAG	7 cut(s)	52, 108, 482, 555, 665, 1213, 1467
Hsp92II	CATG	6 cut(s)	128, 299, 313, 1276, 1480, 1493
HspAI	GCGC	1 cut(s)	314
Ksp22I	TGATCA	1 cut(s)	1246
Kzo9I	GATC	2 cut(s)	982, 1246
LmnI	GCTCC	1 cut(s)	1021
Lsp1109I	GCAGC	2 cut(s)	108, 1090
LweI	GCATC	3 cut(s)	370, 948, 1487
MaeI	CTAG	2 cut(s)	179, 1518
MaeIII	GTNAC	5 cut(s)	30, 205, 270, 997, 1408
MalI	GATC	2 cut(s)	984, 1248
MboI	GATC	2 cut(s)	982, 1246
MboII	GAAGA	7 cut(s)	55, 508, 664, 733, 755, 1060, 1163
MhlI	GDGCHC	1 cut(s)	671
MluCI	AATT	9 cut(s)	167, 243, 352, 375, 459, 519, 892, 1136, 1561
MlyI	GAGTC	1 cut(s)	507
MmeI	TCCRAC	3 cut(s)	113, 594, 804
Mph1103I	ATGCAT	1 cut(s)	1330
MseI	TTAA	7 cut(s)	374, 462, 636, 891, 929, 1323, 1394
MslI	CAYNNNNRTG	1 cut(s)	1481
MwoI	GCNNNNNNNGC	2 cut(s)	320, 1172
NcoI	CCATGG	1 cut(s)	309
NdeII	GATC	2 cut(s)	982, 1246
NlaIII	CATG	6 cut(s)	128, 299, 313, 1276, 1480, 1493
NlaIV	GGNNCC	1 cut(s)	1464
NmeAIII	GCCGAG	2 cut(s)	1097, 1306
NmuCI	GTSAC	1 cut(s)	30
NsiI	ATGCAT	1 cut(s)	1330
NspI	RCATGY	3 cut(s)	128, 299, 1493
PaeI	GCATGC	1 cut(s)	128
PceI	AGGCCT	1 cut(s)	874
PfeI	GAWTC	1 cut(s)	1287
PflFI	GACNNNGTC	1 cut(s)	10
PkrI	GCNGC	2 cut(s)	123, 1105
PleI	GAGTC	1 cut(s)	507
PpsI	GAGTC	1 cut(s)	507
PpuMI	RGGWCCY	1 cut(s)	1462
PshBI	ATTAAT	2 cut(s)	636, 1323
Psp124BI	GAGCTC	1 cut(s)	671
Psp5II	RGGWCCY	1 cut(s)	1462
PspFI	CCCAGC	3 cut(s)	149, 605, 1230
PspN4I	GGNNCC	1 cut(s)	1464
PspPI	GGNCC	3 cut(s)	937, 1462, 1546
PspPPI	RGGWCCY	1 cut(s)	1462
PstNI	CAGNNNCTG	1 cut(s)	209
PsyI	GACNNNGTC	1 cut(s)	10
RsaI	GTAC	3 cut(s)	553, 680, 1263
RsaNI	GTAC	3 cut(s)	552, 679, 1262
RseI	CAYNNNNRTG	1 cut(s)	1481
SacI	GAGCTC	1 cut(s)	671
SaqAI	TTAA	7 cut(s)	374, 462, 636, 891, 929, 1323, 1394
SatI	GCNGC	2 cut(s)	122, 1104
Sau3AI	GATC	2 cut(s)	982, 1246
Sau96I	GGNCC	3 cut(s)	937, 1462, 1546
ScaI	AGTACT	1 cut(s)	1263
SchI	GAGTC	1 cut(s)	507
SduI	GDGCHC	1 cut(s)	671
SfaNI	GCATC	3 cut(s)	370, 948, 1487
SinI	GGWCC	2 cut(s)	1462, 1546
SmiMI	CAYNNNNRTG	1 cut(s)	1481
SmlI	CTYRAG	1 cut(s)	941
SmoI	CTYRAG	1 cut(s)	941
SphI	GCATGC	1 cut(s)	128
Sse9I	AATT	9 cut(s)	167, 243, 352, 375, 459, 519, 892, 1136, 1561
SseBI	AGGCCT	1 cut(s)	874
SsiI	CCGC	1 cut(s)	649
SspMI	CTAG	2 cut(s)	179, 1518
SstI	GAGCTC	1 cut(s)	671
StuI	AGGCCT	1 cut(s)	874
StyI	CCWWGG	1 cut(s)	309
TaaI	ACNGT	3 cut(s)	683, 906, 1255
TaqI	TCGA	5 cut(s)	346, 732, 884, 1290, 1441
TasI	AATT	9 cut(s)	167, 243, 352, 375, 459, 519, 892, 1136, 1561
TatI	WGTACW	2 cut(s)	678, 1261
TfiI	GAWTC	1 cut(s)	1287
Tru1I	TTAA	7 cut(s)	374, 462, 636, 891, 929, 1323, 1394
Tru9I	TTAA	7 cut(s)	374, 462, 636, 891, 929, 1323, 1394
TscAI	CASTG	2 cut(s)	45, 917
TseFI	GTSAC	1 cut(s)	30
TseI	GCWGC	2 cut(s)	121, 1103
Tsp45I	GTSAC	1 cut(s)	30
TspDTI	ATGAA	5 cut(s)	659, 843, 869, 1442, 1530
TspRI	CASTG	2 cut(s)	45, 917
Tth111I	GACNNNGTC	1 cut(s)	10
VpaK11BI	GGWCC	2 cut(s)	1462, 1546
VspI	ATTAAT	2 cut(s)	636, 1323
XagI	CCTNNNNNAGG	1 cut(s)	1427
XapI	RAATTY	3 cut(s)	167, 243, 1136
XceI	RCATGY	3 cut(s)	128, 299, 1493
XmiI	GTMKAC	1 cut(s)	1345
XspI	CTAG	2 cut(s)	179, 1518
ZrmI	AGTACT	1 cut(s)	1263
Zsp2I	ATGCAT	1 cut(s)	1330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.