MD01G1232900.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002642
Physical Location & Seq
Forward (+)
32120265 .. 32122957
2693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1232900.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGGCAATGCTGGTGCAGTCCATTTGGTGTCGTAACTCACCTTCACTTTCATCCGCAGGCAGCTTCGCAATCACTCCACTCTCTCTCTTCTGTCCGAGATGCCTCAAACACTCCGAGACGTACAATTTCCACGCTTCTTTCACTCACTCGAATCGTACCAAGAACGCGAAAGATTTCAAATTTACAGCACCCAGTGACGAAAACACTTCAATTCCGAACTACCTAATTGCTCAAACCACCGGAGAGGAACTTTCCGGCGAATCATACAACAATTTGATAAGTGGGTTCTGCAGAGCAGGGCATATCGACCAGGCAATGGCTGTTCTTGCAGAAATGGAAGCTCTCGGCGTCCGACCCAATTCGATGTCTTACGCTCATTTGATTGAGGGCCTCGGGAGCAATGGCAGGACTTTGGAGGCCGATGTGTTGCTTCAGGAAATGATATGTTCTGGGTTGAGGCCAAGAATCAGGGTTTACAATGTTATGCTCAGAGGGTGTTTGAAGAAAGGCCTCTTAGAACTTGCAACTAGAGTTTTGGCAGTAATGGGCGATTTGGTTGCGGAGAAAAATGAAGAGACGTATGAGATTCTTCTTGATTACTATGTCAGTGCCGGGAGGTTGGAAGATACTTGGTCAACGATTAATGAGATGAAGCGGAAGCGGTTTCGGTTGAGCTCGTTTGTGTATAGCAAGGTTATAGGACTTTACAGGGACAATGGGATGTGGAAGAAGGCAATGGACATTGTGGGAGAGATAAGGGAGATGGGGATGGCATTGGATAAACAGATTTTCAACAGCATTATTGATACGTTTGGGAAATATGGTGAATTGGACGAAGCTTTGGAAGTATTTGATAAGATGAAACAAGAAGGTGTGAAGCCTGATATAACGACATTTAATTCATTGATAAGGTGGCATTGTAAGGCAGGGAATATAAGCAAGGCACTTGAGTTGTTTACAGAGATGCAGGAACAGGGGTTGTATCCTGATCCAAAGATCTTTGTCACCGTTATCAGCCGATTGGGGGAGCAGGGGAAGTGGGATGTGATCCAGAAGACTTTTGAGAATATGAAACGCCGAGGGCATAAGAAAAGTGGGATAATTTATGCTGTTTTGGTTGATATTTATGGGCAGTATGGAAAATTTCAGGATGCTGAAGAGTGTATATCTGCCCTAAAAGCTGAAGGTCTTATTCCTTCAGCTAGCATGTTTTGTGTCTTAGCAAATGCCTACGCTCAGCAGGGATTATGTCATCAGACAGTTAAGGTACTCCAGCTTATGGAAGCTGAGGGAATCGAACCAAACGTCATAATGCTGAATGTGCTGATCAATGCATTCGGTGTTGCTGGTAGATATTTAGAGGCGTTATCCATTTATCACTATATAAAAGAAAGTGGTTTTAGCCCTGATGTGGTTACTTATACTACACTTATGAAGGCGTTTAGTCGGGCAAGGAAGTTTGATAAGGTCCTGGAGATATACAAGGAAATGGAACGCGCCGGATGCACTCCGGATAGAAAGGCTAGACAGGTGTTACAAGTTGCTTTAATGGTCCTTCAACAGAGGTATTGTGAGTAA

Protein Analysis

526

Amino Acids

59.24

Weight (kDa)

7.5

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 78 - 219 8.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 81 - 132 4.7e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 88 - 131 2.5e-11 PPR repeat family
PPR_1 PF12854 88 - 113 2e-08 PPR repeat
PPR PF01535 88 - 117 7.1e-07 PPR repeat
PPR_2 PF13041 119 - 168 3.9e-07 PPR repeat family
PPR_2 PF13041 190 - 233 1.6e-06 PPR repeat family
TPR_24 PF23276 238 - 325 4.3e-08 Fungal tetratrico peptide repeats
PPR_long PF17177 244 - 330 4.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 248 - 304 7.9e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 256 - 288 1.8e-06 PPR repeat
PPR_2 PF13041 263 - 304 5.6e-12 PPR repeat family
PPR PF01535 263 - 292 7.3e-07 PPR repeat
PPR_3 PF13812 283 - 337 8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 291 - 323 1.8e-10 PPR repeat
PPR_2 PF13041 294 - 330 1.3e-10 PPR repeat family
PPR PF01535 298 - 327 2.1e-08 PPR repeat
TPR_24 PF23276 335 - 460 3.8e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 392 - 447 5.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 434 - 482 5.2e-12 PPR repeat family
PPR_3 PF13812 458 - 505 1e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 469 - 506 1.7e-10 PPR repeat family
PPR PF01535 472 - 502 3.5e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 316, 1279
AccII CGCG 2 cut(s) 167, 1497
AccIII TCCGGA 1 cut(s) 1510
AciI CCGC 4 cut(s) 54, 560, 655, 661
AclWI GGATC 2 cut(s) 983, 1042
AcsI RAATTY 2 cut(s) 179, 1142
AcuI CTGAAG 4 cut(s) 416, 1176, 1182, 1203
AcyI GRCGYC 1 cut(s) 348
AdeI CACNNNGTG 1 cut(s) 194
AfaI GTAC 3 cut(s) 122, 157, 1269
AfiI CCNNNNNNNGG 4 cut(s) 316, 1024, 1279, 1411
AgsI TTSAA 5 cut(s) 178, 210, 502, 793, 1559
AjnI CCWGG 2 cut(s) 309, 1470
AluBI AGCT 8 cut(s) 63, 341, 675, 839, 1181, 1202, 1276, 1286
AluI AGCT 8 cut(s) 63, 341, 675, 839, 1181, 1202, 1276, 1286
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 2 cut(s) 110, 569
AlwI GGATC 2 cut(s) 983, 1042
Ama87I CYCGRG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 1510
AoxI GGCC 4 cut(s) 388, 417, 458, 508
ApeKI GCWGC 1 cut(s) 60
ApoI RAATTY 2 cut(s) 179, 1142
ArsI GACNNNNNNTTYG 2 cut(s) 1290, 1322
AseI ATTAAT 1 cut(s) 642
Asp700I GAANNNNTTC 1 cut(s) 173
AspLEI GCGC 1 cut(s) 1499
AspS9I GGNCC 3 cut(s) 388, 1468, 1552
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 3 cut(s) 30, 836, 997
AsuNHI GCTAGC 1 cut(s) 1202
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 2 cut(s) 1468, 1552
BanII GRGCYC 1 cut(s) 677
BarI GAAGNNNNNNTAC 2 cut(s) 25, 57
BbsI GAAGAC 1 cut(s) 1061
Bbv12I GWGCWC 1 cut(s) 677
BbvCI CCTCAGC 1 cut(s) 1287
BbvI GCAGC 1 cut(s) 72
BccI CCATC 2 cut(s) 757, 763
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 2 cut(s) 311, 1472
BciVI GTATCC 1 cut(s) 993
BclI TGATCA 1 cut(s) 1326
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 2 cut(s) 110, 569
BfaI CTAG 3 cut(s) 528, 1203, 1524
BfmI CTRYAG 1 cut(s) 289
BfuI GTATCC 1 cut(s) 993
BglII AGATCT 1 cut(s) 996
BisI GCNGC 1 cut(s) 61
BlpI GCTNAGC 1 cut(s) 1236
BlsI GCNGC 1 cut(s) 62
Bme1390I CCNGG 3 cut(s) 311, 613, 1472
Bme18I GGWCC 2 cut(s) 1468, 1552
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 388, 1468, 1552
BmrFI CCNGG 3 cut(s) 311, 613, 1472
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 4 cut(s) 89, 954, 1141, 1493
BmtI GCTAGC 1 cut(s) 1206
BmuI ACTGGG 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 1061
BpmI CTGGAG 2 cut(s) 1256, 1493
Bpu10I CCTNAGC 1 cut(s) 1287
Bpu1102I GCTNAGC 1 cut(s) 1236
BpuEI CTTGAG 1 cut(s) 968
BpuMI CCSGG 1 cut(s) 613
BsaHI GRCGYC 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 391, 1078
BsaWI WCCGGW 2 cut(s) 239, 1510
Bsc4I CCNNNNNNNGG 4 cut(s) 316, 1024, 1279, 1411
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 4 cut(s) 12, 321, 406, 741
BseAI TCCGGA 1 cut(s) 1510
BseBI CCWGG 2 cut(s) 311, 1472
BseDI CCNNGG 2 cut(s) 391, 1078
BseGI GGATG 6 cut(s) 50, 726, 774, 1048, 1156, 1508
BseLI CCNNNNNNNGG 4 cut(s) 316, 1024, 1279, 1411
BseMI GCAATG 4 cut(s) 12, 321, 406, 741
BseMII CTCAG 3 cut(s) 502, 1250, 1278
BseNI ACTGG 1 cut(s) 192
BseXI GCAGC 1 cut(s) 72
BsgI GTGCAG 1 cut(s) 35
Bsh1236I CGCG 2 cut(s) 167, 1497
BshFI GGCC 4 cut(s) 390, 419, 460, 510
BsiHKAI GWGCWC 1 cut(s) 677
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 5 cut(s) 240, 255, 612, 1500, 1511
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 4 cut(s) 316, 1024, 1279, 1411
BsmAI GTCTC 2 cut(s) 110, 569
BsmBI CGTCTC 2 cut(s) 110, 569
BsmFI GGGAC 1 cut(s) 725
BsmI GAATGC 1 cut(s) 1334
BsnI GGCC 4 cut(s) 390, 419, 460, 510
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 1 cut(s) 677
Bsp13I TCCGGA 1 cut(s) 1510
Bsp143I GATC 4 cut(s) 988, 996, 1047, 1326
Bsp1720I GCTNAGC 1 cut(s) 1236
BspACI CCGC 4 cut(s) 54, 560, 655, 661
BspANI GGCC 4 cut(s) 390, 419, 460, 510
BspCNI CTCAG 3 cut(s) 501, 1249, 1279
BspEI TCCGGA 1 cut(s) 1510
BspFNI CGCG 2 cut(s) 167, 1497
BspMAI CTGCAG 1 cut(s) 293
BspOI GCTAGC 1 cut(s) 1206
BspPI GGATC 2 cut(s) 983, 1042
BsrDI GCAATG 4 cut(s) 12, 321, 406, 741
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 2 cut(s) 391, 1078
BssMI GATC 4 cut(s) 988, 996, 1047, 1326
BssNI GRCGYC 1 cut(s) 348
Bst2UI CCWGG 2 cut(s) 311, 1472
Bst4CI ACNGT 2 cut(s) 1009, 1261
Bst6I CTCTTC 3 cut(s) 92, 567, 1152
BstACI GRCGYC 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 58, 1204
BstDEI CTNAG 5 cut(s) 488, 514, 1219, 1236, 1287
BstF5I GGATG 6 cut(s) 50, 726, 774, 1048, 1156, 1508
BstFNI CGCG 2 cut(s) 167, 1497
BstHHI GCGC 1 cut(s) 1499
BstKTI GATC 4 cut(s) 991, 999, 1050, 1329
BstMAI GTCTC 2 cut(s) 110, 569
BstMBI GATC 4 cut(s) 988, 996, 1047, 1326
BstMWI GCNNNNNNNGC 5 cut(s) 326, 396, 1178, 1321, 1503
BstNI CCWGG 2 cut(s) 311, 1472
BstNSI RCATGY 1 cut(s) 1210
BstSCI CCNGG 3 cut(s) 309, 611, 1470
BstSFI CTRYAG 1 cut(s) 289
BstUI CGCG 2 cut(s) 167, 1497
BstV1I GCAGC 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 1061
BstX2I RGATCY 1 cut(s) 996
BstYI RGATCY 1 cut(s) 996
BsuI GTATCC 1 cut(s) 993
BsuRI GGCC 4 cut(s) 390, 419, 460, 510
BtsCI GGATG 6 cut(s) 50, 726, 774, 1048, 1156, 1508
BtsIMutI CAGTG 2 cut(s) 199, 613
Cac8I GCNNGC 2 cut(s) 58, 1204
CfoI GCGC 1 cut(s) 1499
Cfr13I GGNCC 3 cut(s) 388, 1468, 1552
CseI GACGC 1 cut(s) 337
Csp6I GTAC 3 cut(s) 121, 156, 1268
CviAII CATG 1 cut(s) 1207
CviQI GTAC 3 cut(s) 121, 156, 1268
DdeI CTNAG 5 cut(s) 488, 514, 1219, 1236, 1287
DpnI GATC 4 cut(s) 990, 998, 1049, 1328
DpnII GATC 4 cut(s) 988, 996, 1047, 1326
DraIII CACNNNGTG 1 cut(s) 194
Eam1104I CTCTTC 3 cut(s) 92, 567, 1152
EarI CTCTTC 3 cut(s) 92, 567, 1152
Ecl136II GAGCTC 1 cut(s) 675
Eco147I AGGCCT 1 cut(s) 510
Eco24I GRGCYC 1 cut(s) 677
Eco47I GGWCC 2 cut(s) 1468, 1552
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 4 cut(s) 416, 1176, 1182, 1203
Eco88I CYCGRG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 675
EcoO109I RGGNCCY 2 cut(s) 388, 1468
EcoRII CCWGG 2 cut(s) 309, 1470
EcoT22I ATGCAT 1 cut(s) 1336
EcoT38I GRGCYC 1 cut(s) 677
Esp3I CGTCTC 2 cut(s) 110, 569
FaeI CATG 1 cut(s) 1210
FaqI GGGAC 1 cut(s) 725
FatI CATG 1 cut(s) 1206
FbaI TGATCA 1 cut(s) 1326
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 6 cut(s) 37, 733, 781, 1055, 1163, 1515
FriOI GRGCYC 1 cut(s) 677
Fsp4HI GCNGC 1 cut(s) 61
FspBI CTAG 3 cut(s) 528, 1203, 1524
GlaI GCGC 1 cut(s) 1498
GluI GCNGC 1 cut(s) 61
GsuI CTGGAG 2 cut(s) 1256, 1493
HaeIII GGCC 4 cut(s) 390, 419, 460, 510
HapII CCGG 5 cut(s) 240, 255, 612, 1500, 1511
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 1499
Hin1I GRCGYC 1 cut(s) 348
Hin1II CATG 1 cut(s) 1210
Hin6I GCGC 1 cut(s) 1497
HinP1I GCGC 1 cut(s) 1497
HincII GTYRAC 1 cut(s) 636
HindII GTYRAC 1 cut(s) 636
HindIII AAGCTT 1 cut(s) 837
HinfI GANTC 5 cut(s) 151, 260, 465, 586, 1293
HpaII CCGG 5 cut(s) 240, 255, 612, 1500, 1511
HphI GGTGA 3 cut(s) 30, 836, 997
Hpy166II GTNNAC 3 cut(s) 475, 636, 957
Hpy188I TCNGA 6 cut(s) 96, 115, 216, 353, 491, 1257
Hpy188III TCNNGA 7 cut(s) 394, 434, 593, 986, 1051, 1148, 1511
Hpy8I GTNNAC 3 cut(s) 475, 636, 957
HpyAV CCTTC 7 cut(s) 51, 724, 863, 1178, 1206, 1429, 1565
HpyCH4III ACNGT 2 cut(s) 1009, 1261
HpyCH4IV ACGT 4 cut(s) 119, 578, 809, 1305
HpyCH4V TGCA 7 cut(s) 16, 291, 329, 524, 967, 1334, 1506
HpyF10VI GCNNNNNNNGC 5 cut(s) 326, 396, 1178, 1321, 1503
HpyF3I CTNAG 5 cut(s) 488, 514, 1219, 1236, 1287
HpySE526I ACGT 4 cut(s) 119, 578, 809, 1305
Hsp92I GRCGYC 1 cut(s) 348
Hsp92II CATG 1 cut(s) 1210
HspAI GCGC 1 cut(s) 1497
Kpn2I TCCGGA 1 cut(s) 1510
Ksp22I TGATCA 1 cut(s) 1326
Kzo9I GATC 4 cut(s) 988, 996, 1047, 1326
LmnI GCTCC 2 cut(s) 396, 1027
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 4 cut(s) 89, 954, 1141, 1493
MaeI CTAG 3 cut(s) 528, 1203, 1524
MaeII ACGT 4 cut(s) 119, 578, 809, 1305
MaeIII GTNAC 5 cut(s) 32, 194, 1003, 1414, 1533
MalI GATC 4 cut(s) 990, 998, 1049, 1328
MboI GATC 4 cut(s) 988, 996, 1047, 1326
MboII GAAGA 8 cut(s) 79, 514, 581, 584, 635, 739, 1066, 1169
MflI RGATCY 1 cut(s) 996
MhlI GDGCHC 1 cut(s) 677
MmeI TCCRAC 2 cut(s) 376, 600
Mph1103I ATGCAT 1 cut(s) 1336
MroI TCCGGA 1 cut(s) 1510
MroXI GAANNNNTTC 1 cut(s) 173
MseI TTAA 4 cut(s) 642, 897, 1263, 1547
MspI CCGG 5 cut(s) 240, 255, 612, 1500, 1511
MspR9I CCNGG 3 cut(s) 311, 613, 1472
Mva1269I GAATGC 1 cut(s) 1334
MvaI CCWGG 2 cut(s) 311, 1472
MvnI CGCG 2 cut(s) 167, 1497
MwoI GCNNNNNNNGC 5 cut(s) 326, 396, 1178, 1321, 1503
NciI CCSGG 1 cut(s) 613
NdeII GATC 4 cut(s) 988, 996, 1047, 1326
NheI GCTAGC 1 cut(s) 1202
NlaIII CATG 1 cut(s) 1210
NmeAIII GCCGAG 2 cut(s) 324, 1103
NmuCI GTSAC 2 cut(s) 194, 1003
NsiI ATGCAT 1 cut(s) 1336
NspI RCATGY 1 cut(s) 1210
PceI AGGCCT 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 1302
PctI GAATGC 1 cut(s) 1334
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 5 cut(s) 151, 260, 465, 586, 1293
PflMI CCANNNNNTGG 2 cut(s) 316, 1279
PfoI TCCNGGA 1 cut(s) 1470
PkrI GCNGC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 1468
PshBI ATTAAT 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 1468
Psp6I CCWGG 2 cut(s) 309, 1470
PspGI CCWGG 2 cut(s) 309, 1470
PspPI GGNCC 3 cut(s) 388, 1468, 1552
PspPPI RGGWCCY 1 cut(s) 1468
PstI CTGCAG 1 cut(s) 293
PsuI RGATCY 1 cut(s) 996
RsaI GTAC 3 cut(s) 122, 157, 1269
RsaNI GTAC 3 cut(s) 121, 156, 1268
SacI GAGCTC 1 cut(s) 677
SaqAI TTAA 4 cut(s) 642, 897, 1263, 1547
SatI GCNGC 1 cut(s) 61
Sau3AI GATC 4 cut(s) 988, 996, 1047, 1326
Sau96I GGNCC 3 cut(s) 388, 1468, 1552
ScrFI CCNGG 3 cut(s) 311, 613, 1472
SduI GDGCHC 1 cut(s) 677
SfaNI GCATC 4 cut(s) 89, 954, 1141, 1493
SfcI CTRYAG 1 cut(s) 289
SinI GGWCC 2 cut(s) 1468, 1552
SmlI CTYRAG 1 cut(s) 947
SmoI CTYRAG 1 cut(s) 947
SseBI AGGCCT 1 cut(s) 510
SsiI CCGC 4 cut(s) 54, 560, 655, 661
SspMI CTAG 3 cut(s) 528, 1203, 1524
SstI GAGCTC 1 cut(s) 677
StuI AGGCCT 1 cut(s) 510
StyD4I CCNGG 3 cut(s) 309, 611, 1470
TaaI ACNGT 2 cut(s) 1009, 1261
TaiI ACGT 4 cut(s) 122, 581, 812, 1308
TaqI TCGA 4 cut(s) 149, 306, 362, 1296
TfiI GAWTC 5 cut(s) 151, 260, 465, 586, 1293
Tru1I TTAA 4 cut(s) 642, 897, 1263, 1547
Tru9I TTAA 4 cut(s) 642, 897, 1263, 1547
TscAI CASTG 2 cut(s) 199, 613
TseFI GTSAC 2 cut(s) 194, 1003
TseI GCWGC 1 cut(s) 60
Tsp45I GTSAC 2 cut(s) 194, 1003
TspDTI ATGAA 7 cut(s) 39, 585, 665, 875, 891, 1085, 1448
TspRI CASTG 2 cut(s) 199, 613
Van91I CCANNNNNTGG 2 cut(s) 316, 1279
VpaK11BI GGWCC 2 cut(s) 1468, 1552
VspI ATTAAT 1 cut(s) 642
XapI RAATTY 2 cut(s) 179, 1142
XceI RCATGY 1 cut(s) 1210
XmnI GAANNNNTTC 1 cut(s) 173
XspI CTAG 3 cut(s) 528, 1203, 1524
Zsp2I ATGCAT 1 cut(s) 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.