MD00G1073400.v1.1

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003160
Physical Location & Seq
Forward (+)
14835486 .. 14837126
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1073400.v1.1.491

Sequence Viewer

Length: 573 bp
ATGCTGTTAGGGAAGCGCCCACGCCCTCCAATCAGGAGAACAACAAGCATGACTGGGATTACCGTTGATATGAGCGACGTGGAAGGCCAGGAACCGACTGATGAACTCCAAAACCCCGCCGCCATGAACGATGTGCATGCAGTTGCAGTTGCAGCGACGGTGGTGGACGCACAAGGACCTGCTCCTGTGATTAATAATAATAATTATTATGATATTGAAAAGATGAACATGAACAACTTAGGGTACCATGAAGAGCACCGTTTCTTGGCCATGTTATCACCCAGAAACAACCACACAAGGAGGAATTCCCCATCTCATGATGTTGTGGAGACCGCTCACTTCCTTCGCACATGTGGCCTCTGCAAGCGCCAGCTCGCTTCAGGCCGTGACATCTATATGTATAGGGGAGACACAGCGTTTTGTAGTCTAGAATGTAGAGAGCAACAGATGAAGCAGGATGAGAGAAAAGAGAAGTGTAAAGCTGCGTCCTCAAAGATAGAGGACCGCCATGCGGCGTCACGATCCACCTCCAAGGCCTCCTCCGGCAAGGGTCAGACTGTGGCGGCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

191

Amino Acids

21.12

Weight (kDa)

8.61

Isoelectric Point (pI)

59.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 107 - 156 2.5e-26 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
Acc65I GGTACC 1 cut(s) 243
AccB1I GGYRCC 1 cut(s) 243
AccBSI CCGCTC 1 cut(s) 335
AciI CCGC 6 cut(s) 117, 120, 333, 505, 512, 563
AclWI GGATC 1 cut(s) 516
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 1 cut(s) 304
AcuI CTGAAG 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 265, 511
AflIII ACRYGT 1 cut(s) 350
AgsI TTSAA 1 cut(s) 218
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 2 cut(s) 373, 482
AluI AGCT 2 cut(s) 373, 482
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 323, 402
AlwI GGATC 1 cut(s) 516
AoxI GGCC 5 cut(s) 85, 267, 355, 382, 534
ApeKI GCWGC 3 cut(s) 152, 482, 566
ApoI RAATTY 1 cut(s) 304
AseI ATTAAT 1 cut(s) 192
Asp718I GGTACC 1 cut(s) 243
AspLEI GCGC 2 cut(s) 18, 369
AspS9I GGNCC 2 cut(s) 176, 502
AsuHPI GGTGA 1 cut(s) 270
AvaII GGWCC 2 cut(s) 176, 502
BalI TGGCCA 1 cut(s) 269
BanI GGYRCC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 3 cut(s) 164, 469, 553
BccI CCATC 1 cut(s) 319
BceAI ACGGC 1 cut(s) 369
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 1 cut(s) 89
BcoDI GTCTC 2 cut(s) 323, 402
BfaI CTAG 1 cut(s) 428
BfoI RGCGCY 2 cut(s) 19, 370
BfuAI ACCTGC 1 cut(s) 187
BisI GCNGC 6 cut(s) 120, 153, 483, 513, 564, 567
BlsI GCNGC 6 cut(s) 121, 154, 484, 514, 565, 568
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 2 cut(s) 176, 502
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 2 cut(s) 176, 502
BmiI GGNNCC 2 cut(s) 93, 245
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 1 cut(s) 63
BmuI ACTGGG 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 1 cut(s) 531
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 511
Bse1I ACTGG 1 cut(s) 58
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 531
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 2 cut(s) 265, 511
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 529
BseXI GCAGC 3 cut(s) 164, 469, 553
BshFI GGCC 5 cut(s) 87, 269, 357, 384, 536
BshNI GGYRCC 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 1 cut(s) 543
BslI CCNNNNNNNGG 2 cut(s) 265, 511
BsmAI GTCTC 2 cut(s) 323, 402
BsnI GGCC 5 cut(s) 87, 269, 357, 384, 536
Bso31I GGTCTC 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 1 cut(s) 521
BspACI CCGC 6 cut(s) 117, 120, 333, 505, 512, 563
BspANI GGCC 5 cut(s) 87, 269, 357, 384, 536
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 2 cut(s) 93, 245
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 1 cut(s) 516
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 243
BspTNI GGTCTC 1 cut(s) 323
BsrBI CCGCTC 1 cut(s) 335
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 531
BssMI GATC 1 cut(s) 521
BssNI GRCGYC 1 cut(s) 515
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 4 cut(s) 64, 160, 260, 559
Bst6I CTCTTC 1 cut(s) 246
BstACI GRCGYC 1 cut(s) 515
BstC8I GCNNGC 4 cut(s) 138, 365, 371, 375
BstDEI CTNAG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 463
BstH2I RGCGCY 2 cut(s) 19, 370
BstHHI GCGC 2 cut(s) 18, 369
BstKTI GATC 1 cut(s) 524
BstMAI GTCTC 2 cut(s) 323, 402
BstMBI GATC 1 cut(s) 521
BstMWI GCNNNNNNNGC 2 cut(s) 152, 354
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 2 cut(s) 140, 354
BstSCI CCNGG 1 cut(s) 87
BstV1I GCAGC 3 cut(s) 164, 469, 553
BsuRI GGCC 5 cut(s) 87, 269, 357, 384, 536
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 463
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 4 cut(s) 138, 365, 371, 375
CciI TCATGA 1 cut(s) 316
CfoI GCGC 2 cut(s) 18, 369
Cfr13I GGNCC 2 cut(s) 176, 502
CseI GACGC 3 cut(s) 176, 474, 504
Csp6I GTAC 1 cut(s) 244
CviAII CATG 9 cut(s) 49, 124, 137, 229, 248, 271, 317, 351, 509
CviJI RGCY 8 cut(s) 87, 269, 357, 373, 384, 482, 536, 566
CviKI_1 RGCY 8 cut(s) 87, 269, 357, 373, 384, 482, 536, 566
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 1 cut(s) 238
DpnI GATC 1 cut(s) 523
DpnII GATC 1 cut(s) 521
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 531
Eco147I AGGCCT 1 cut(s) 536
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 2 cut(s) 176, 502
Eco57I CTGAAG 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 9 cut(s) 52, 127, 140, 232, 251, 274, 320, 354, 512
FatI CATG 9 cut(s) 48, 123, 136, 228, 247, 270, 316, 350, 508
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 6 cut(s) 120, 153, 483, 513, 564, 567
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 6 cut(s) 120, 153, 483, 513, 564, 567
FspBI CTAG 1 cut(s) 428
GlaI GCGC 2 cut(s) 17, 368
GluI GCNGC 6 cut(s) 120, 153, 483, 513, 564, 567
HaeII RGCGCY 2 cut(s) 19, 370
HaeIII GGCC 5 cut(s) 87, 269, 357, 384, 536
HapII CCGG 1 cut(s) 543
HgaI GACGC 3 cut(s) 176, 474, 504
HhaI GCGC 2 cut(s) 18, 369
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 9 cut(s) 52, 127, 140, 232, 251, 274, 320, 354, 512
Hin6I GCGC 2 cut(s) 16, 367
HinP1I GCGC 2 cut(s) 16, 367
HpaII CCGG 1 cut(s) 543
HphI GGTGA 1 cut(s) 270
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 1 cut(s) 555
Hpy188III TCNNGA 4 cut(s) 34, 317, 428, 519
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 2 cut(s) 80, 160
HpyAV CCTTC 2 cut(s) 77, 353
HpyCH4III ACNGT 4 cut(s) 64, 160, 260, 559
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 5 cut(s) 136, 140, 146, 152, 363
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 354
HpyF3I CTNAG 1 cut(s) 238
HpySE526I ACGT 1 cut(s) 78
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 9 cut(s) 52, 127, 140, 232, 251, 274, 320, 354, 512
HspAI GCGC 2 cut(s) 16, 367
KpnI GGTACC 1 cut(s) 247
Kzo9I GATC 1 cut(s) 521
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 3 cut(s) 164, 469, 553
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 386, 516
MalI GATC 1 cut(s) 523
MbiI CCGCTC 1 cut(s) 335
MboI GATC 1 cut(s) 521
MboII GAAGA 1 cut(s) 263
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 2 cut(s) 202, 304
MluNI TGGCCA 1 cut(s) 269
MnlI CCTC 8 cut(s) 36, 294, 368, 493, 499, 538, 547, 550
Mox20I TGGCCA 1 cut(s) 269
MscI TGGCCA 1 cut(s) 269
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 269
MspI CCGG 1 cut(s) 543
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 152, 354
NdeII GATC 1 cut(s) 521
NlaIII CATG 9 cut(s) 52, 127, 140, 232, 251, 274, 320, 354, 512
NlaIV GGNNCC 2 cut(s) 93, 245
NmuCI GTSAC 2 cut(s) 386, 516
NspI RCATGY 2 cut(s) 140, 354
PaeI GCATGC 1 cut(s) 140
PagI TCATGA 1 cut(s) 316
PceI AGGCCT 1 cut(s) 536
PciI ACATGT 1 cut(s) 350
PciSI GCTCTTC 1 cut(s) 246
PkrI GCNGC 6 cut(s) 121, 154, 484, 514, 565, 568
PpuMI RGGWCCY 1 cut(s) 176
PscI ACATGT 1 cut(s) 350
PshBI ATTAAT 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 93, 245
PspPI GGNCC 2 cut(s) 176, 502
PspPPI RGGWCCY 1 cut(s) 176
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 6 cut(s) 120, 153, 483, 513, 564, 567
Sau3AI GATC 1 cut(s) 521
Sau96I GGNCC 2 cut(s) 176, 502
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 258
SetI ASST 5 cut(s) 81, 181, 375, 484, 530
SinI GGWCC 2 cut(s) 176, 502
SmiMI CAYNNNNRTG 1 cut(s) 395
SphI GCATGC 1 cut(s) 140
Sse9I AATT 2 cut(s) 202, 304
SseBI AGGCCT 1 cut(s) 536
SsiI CCGC 6 cut(s) 117, 120, 333, 505, 512, 563
SspMI CTAG 1 cut(s) 428
StuI AGGCCT 1 cut(s) 536
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 4 cut(s) 64, 160, 260, 559
TaiI ACGT 1 cut(s) 81
TasI AATT 2 cut(s) 202, 304
TauI GCSGC 3 cut(s) 122, 515, 566
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseFI GTSAC 2 cut(s) 386, 516
TseI GCWGC 3 cut(s) 152, 482, 566
Tsp45I GTSAC 2 cut(s) 386, 516
TspDTI ATGAA 6 cut(s) 117, 140, 239, 245, 264, 464
VpaK11BI GGWCC 2 cut(s) 176, 502
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 1 cut(s) 304
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 2 cut(s) 140, 354
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.