pycom15g29830

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003160
Physical Location & Seq
Reverse (-)
26909707 .. 26910745
1039 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g29830.1

Sequence Viewer

Length: 573 bp
ATGCTGTTAGGGAAGCGCCCACGCCCTCCAATCAGGAGAACAACAAGCATGACTGGGATTACCGTTGATATGAGCGACGTGCAAGGCCAGGAACCGACTGATGAACTCCAAAACTCCCCCGCCATGAACGATGTGCATGCAGTTGCAGTTGCAGCGACGGTGGTGGACGCACAAGGACCTGCTCCTGTGATTAATAATAATAATTATTATGATGTTGAAAAGATGAACATGAACAACTTAGGGTACCATGAAGAGCACCGTTTCTTGGCCATGCTATCACCCAGAAACAACCACACAAGGAGGAACTCCGCTTCTCATGATGTTGTGGAGACCGCTCACTTCCTTCGCACGTGTGGACTCTGCAAACGCCAACTCGCTTCAGGCCGTGACATCTATATGTATAGGGGAGACGCAGCGTTTTGTAGTCTAGAATGTAGAGAGCAACAGATGAAGCAGGATGAGAGAAAAGAGAAGTGTAAAGCTGCGTCCTCAAAGATAGAGGACCGCCATGCGGCGTCACGATCCACCTCCAAGGCCTCCTCCGGCAAGGGTCAGACCGTGGCGGCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

191

Amino Acids

21.07

Weight (kDa)

8.84

Isoelectric Point (pI)

57.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 105 - 156 4.6e-26 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
Acc65I GGTACC 1 cut(s) 243
AccB1I GGYRCC 1 cut(s) 243
AccBSI CCGCTC 1 cut(s) 335
AciI CCGC 6 cut(s) 120, 309, 333, 505, 512, 563
AclWI GGATC 1 cut(s) 516
AcoI YGGCCR 1 cut(s) 267
AcuI CTGAAG 1 cut(s) 363
AcvI CACGTG 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 265, 511
AflIII ACRYGT 1 cut(s) 350
AgsI TTSAA 1 cut(s) 218
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 1 cut(s) 482
AluI AGCT 1 cut(s) 482
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 323, 402
AlwI GGATC 1 cut(s) 516
AoxI GGCC 4 cut(s) 85, 267, 382, 534
ApeKI GCWGC 4 cut(s) 152, 413, 482, 566
AseI ATTAAT 1 cut(s) 192
Asp718I GGTACC 1 cut(s) 243
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 2 cut(s) 176, 502
AsuHPI GGTGA 1 cut(s) 270
AvaII GGWCC 2 cut(s) 176, 502
BalI TGGCCA 1 cut(s) 269
BanI GGYRCC 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 4 cut(s) 164, 425, 469, 553
BceAI ACGGC 1 cut(s) 369
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 1 cut(s) 89
BcoDI GTCTC 2 cut(s) 323, 402
BfaI CTAG 1 cut(s) 428
BfoI RGCGCY 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 187
BisI GCNGC 6 cut(s) 153, 414, 483, 513, 564, 567
BlsI GCNGC 6 cut(s) 154, 415, 484, 514, 565, 568
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 2 cut(s) 176, 502
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 2 cut(s) 176, 502
BmiI GGNNCC 2 cut(s) 93, 245
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 1 cut(s) 63
BmuI ACTGGG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 351
BsaHI GRCGYC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 531, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 511
Bse1I ACTGG 1 cut(s) 58
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 2 cut(s) 531, 558
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 2 cut(s) 265, 511
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 529
BseXI GCAGC 4 cut(s) 164, 425, 469, 553
BshFI GGCC 4 cut(s) 87, 269, 384, 536
BshNI GGYRCC 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 1 cut(s) 543
BslI CCNNNNNNNGG 2 cut(s) 265, 511
BsmAI GTCTC 2 cut(s) 323, 402
BsmBI CGTCTC 1 cut(s) 402
BsnI GGCC 4 cut(s) 87, 269, 384, 536
Bso31I GGTCTC 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 1 cut(s) 521
BspACI CCGC 6 cut(s) 120, 309, 333, 505, 512, 563
BspANI GGCC 4 cut(s) 87, 269, 384, 536
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 2 cut(s) 93, 245
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 1 cut(s) 516
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 243
BspTNI GGTCTC 1 cut(s) 323
BsrBI CCGCTC 1 cut(s) 335
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 531, 558
BssMI GATC 1 cut(s) 521
BssNI GRCGYC 1 cut(s) 515
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 4 cut(s) 64, 160, 260, 559
Bst6I CTCTTC 1 cut(s) 246
BstACI GRCGYC 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 351
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 238
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 1 cut(s) 463
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 1 cut(s) 524
BstMAI GTCTC 2 cut(s) 323, 402
BstMBI GATC 1 cut(s) 521
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 87
BstV1I GCAGC 4 cut(s) 164, 425, 469, 553
BsuRI GGCC 4 cut(s) 87, 269, 384, 536
BtgI CCRYGG 1 cut(s) 558
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 463
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 1 cut(s) 138
CciI TCATGA 1 cut(s) 316
CfoI GCGC 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 176, 502
CseI GACGC 4 cut(s) 176, 419, 474, 504
Csp6I GTAC 1 cut(s) 244
CviAII CATG 8 cut(s) 49, 124, 137, 229, 248, 271, 317, 509
CviJI RGCY 6 cut(s) 87, 269, 384, 482, 536, 566
CviKI_1 RGCY 6 cut(s) 87, 269, 384, 482, 536, 566
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 1 cut(s) 238
DpnI GATC 1 cut(s) 523
DpnII GATC 1 cut(s) 521
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 531
Eco147I AGGCCT 1 cut(s) 536
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 2 cut(s) 176, 502
Eco57I CTGAAG 1 cut(s) 363
Eco72I CACGTG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 531
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 8 cut(s) 52, 127, 140, 232, 251, 274, 320, 512
FatI CATG 8 cut(s) 48, 123, 136, 228, 247, 270, 316, 508
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 6 cut(s) 153, 414, 483, 513, 564, 567
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 6 cut(s) 153, 414, 483, 513, 564, 567
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 17
GluI GCNGC 6 cut(s) 153, 414, 483, 513, 564, 567
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 4 cut(s) 87, 269, 384, 536
HapII CCGG 1 cut(s) 543
HgaI GACGC 4 cut(s) 176, 419, 474, 504
HhaI GCGC 1 cut(s) 18
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 8 cut(s) 52, 127, 140, 232, 251, 274, 320, 512
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HinfI GANTC 1 cut(s) 357
HpaII CCGG 1 cut(s) 543
HphI GGTGA 1 cut(s) 270
Hpy166II GTNNAC 2 cut(s) 166, 356
Hpy188I TCNGA 1 cut(s) 555
Hpy188III TCNNGA 4 cut(s) 34, 317, 428, 519
Hpy8I GTNNAC 2 cut(s) 166, 356
Hpy99I CGWCG 2 cut(s) 80, 160
HpyAV CCTTC 1 cut(s) 353
HpyCH4III ACNGT 4 cut(s) 64, 160, 260, 559
HpyCH4IV ACGT 2 cut(s) 78, 350
HpyCH4V TGCA 6 cut(s) 82, 136, 140, 146, 152, 363
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 1 cut(s) 238
HpySE526I ACGT 2 cut(s) 78, 350
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 8 cut(s) 52, 127, 140, 232, 251, 274, 320, 512
HspAI GCGC 1 cut(s) 16
KpnI GGTACC 1 cut(s) 247
Kzo9I GATC 1 cut(s) 521
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 4 cut(s) 164, 425, 469, 553
MaeI CTAG 1 cut(s) 428
MaeII ACGT 2 cut(s) 78, 350
MaeIII GTNAC 2 cut(s) 386, 516
MalI GATC 1 cut(s) 523
MbiI CCGCTC 1 cut(s) 335
MboI GATC 1 cut(s) 521
MboII GAAGA 1 cut(s) 263
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 1 cut(s) 202
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 1 cut(s) 351
MnlI CCTC 7 cut(s) 36, 294, 493, 499, 538, 547, 550
Mox20I TGGCCA 1 cut(s) 269
MscI TGGCCA 1 cut(s) 269
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 269
MspI CCGG 1 cut(s) 543
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 1 cut(s) 152
NdeII GATC 1 cut(s) 521
NlaIII CATG 8 cut(s) 52, 127, 140, 232, 251, 274, 320, 512
NlaIV GGNNCC 2 cut(s) 93, 245
NmuCI GTSAC 2 cut(s) 386, 516
NspI RCATGY 1 cut(s) 140
PaeI GCATGC 1 cut(s) 140
PagI TCATGA 1 cut(s) 316
PceI AGGCCT 1 cut(s) 536
PciSI GCTCTTC 1 cut(s) 246
PkrI GCNGC 6 cut(s) 154, 415, 484, 514, 565, 568
PleI GAGTC 1 cut(s) 351
PmaCI CACGTG 1 cut(s) 351
PmlI CACGTG 1 cut(s) 351
PpsI GAGTC 1 cut(s) 351
Ppu21I YACGTR 1 cut(s) 351
PpuMI RGGWCCY 1 cut(s) 176
PshBI ATTAAT 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 87
PspCI CACGTG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 93, 245
PspPI GGNCC 2 cut(s) 176, 502
PspPPI RGGWCCY 1 cut(s) 176
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 6 cut(s) 153, 414, 483, 513, 564, 567
Sau3AI GATC 1 cut(s) 521
Sau96I GGNCC 2 cut(s) 176, 502
SchI GAGTC 1 cut(s) 351
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 258
SetI ASST 5 cut(s) 81, 181, 353, 484, 530
SinI GGWCC 2 cut(s) 176, 502
SmiMI CAYNNNNRTG 1 cut(s) 395
SphI GCATGC 1 cut(s) 140
Sse9I AATT 1 cut(s) 202
SseBI AGGCCT 1 cut(s) 536
SsiI CCGC 6 cut(s) 120, 309, 333, 505, 512, 563
SspMI CTAG 1 cut(s) 428
StuI AGGCCT 1 cut(s) 536
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 4 cut(s) 64, 160, 260, 559
TaiI ACGT 2 cut(s) 81, 353
TasI AATT 1 cut(s) 202
TauI GCSGC 2 cut(s) 515, 566
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseFI GTSAC 2 cut(s) 386, 516
TseI GCWGC 4 cut(s) 152, 413, 482, 566
Tsp45I GTSAC 2 cut(s) 386, 516
TspDTI ATGAA 6 cut(s) 117, 140, 239, 245, 264, 464
VpaK11BI GGWCC 2 cut(s) 176, 502
VspI ATTAAT 1 cut(s) 192
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 1 cut(s) 140
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.