MD01G1025300.v1.1

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003160
Physical Location & Seq
Reverse (-)
9601689 .. 9603086
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1025300.v1.1.491

Sequence Viewer

Length: 585 bp
ATGTTGTTAGGGAAGCGTCCACGCCCTCCGATGAAGAGAACAGCAAGCATGACTGGGATCACCATTGATCTGACGGACGTGGAAGGCCAGGAACCGACTTATGATCTCCAAAACCCCTCCGCCATCAACGATGAGCATGCAGTTGCAGTTGCAGCAACGGTGGTGGGCCCACAAGAACCTTGTCCTGTGATTAACAATAATCATAATTCTAATTATAATATAGAAAAGATGAACATGAACAACATCAGCTTAGGGAACTATGAAGAGCACCGTTTCTTGACCATGTTGTCACCCAGAAACAACCACAGAAGAAGGAACTCGGCTTCTCAAGATGTTGTGGAGACCGCTCACTTCCTTCGCACGTGTGGCCTCTGCAAACGCCAGCTCGCTTCAGGCCGCGACATCTATATGTATAGGGGAGACACAGCGTTTTGTAGTCTAGAATGTAGAGAGCAACAGATTAAGCAGGATGAGAGAATAGAGAAGTGTAAAGCTGTGTCCTCAAAGAAAGAGGACCACCATGCGTCGTCACGATCCACCTCCAAGACCTCCTCCGGCAAAAGCCAGACCGTGGCGGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

195

Amino Acids

21.69

Weight (kDa)

8.75

Isoelectric Point (pI)

64.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 110 - 159 9.5e-26 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
AasI GACNNNNNNGTC 1 cut(s) 286
AccB7I CCANNNNNTGG 1 cut(s) 571
AccBSI CCGCTC 1 cut(s) 347
AccII CGCG 1 cut(s) 399
AciI CCGC 4 cut(s) 120, 345, 397, 575
AclWI GGATC 2 cut(s) 65, 528
AcuI CTGAAG 1 cut(s) 375
AcvI CACGTG 1 cut(s) 363
AfiI CCNNNNNNNGG 1 cut(s) 571
AflIII ACRYGT 1 cut(s) 362
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 3 cut(s) 249, 385, 494
AluI AGCT 3 cut(s) 249, 385, 494
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 2 cut(s) 335, 414
AlwI GGATC 2 cut(s) 65, 528
AoxI GGCC 4 cut(s) 85, 166, 367, 394
ApaI GGGCCC 1 cut(s) 170
ApeKI GCWGC 2 cut(s) 152, 578
AspS9I GGNCC 3 cut(s) 166, 167, 514
AsuHPI GGTGA 2 cut(s) 52, 282
AvaII GGWCC 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 170
BbrPI CACGTG 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 2 cut(s) 164, 565
BccI CCATC 1 cut(s) 131
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 1 cut(s) 89
BcoDI GTCTC 2 cut(s) 335, 414
BfaI CTAG 1 cut(s) 440
BisI GCNGC 4 cut(s) 153, 397, 576, 579
BlsI GCNGC 4 cut(s) 154, 398, 577, 580
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 514
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 166, 167, 514
BmiI GGNNCC 2 cut(s) 93, 168
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 1 cut(s) 63
BmuI ACTGGG 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 250
BpuEI CTTGAG 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 570
Bsc4I CCNNNNNNNGG 1 cut(s) 571
Bse1I ACTGG 1 cut(s) 58
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 1 cut(s) 571
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 541
BseSI GKGCMC 1 cut(s) 170
BseXI GCAGC 2 cut(s) 164, 565
Bsh1236I CGCG 1 cut(s) 399
BshFI GGCC 4 cut(s) 87, 168, 369, 396
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 1 cut(s) 555
BslI CCNNNNNNNGG 1 cut(s) 571
BsmAI GTCTC 2 cut(s) 335, 414
BsnI GGCC 4 cut(s) 87, 168, 369, 396
Bso31I GGTCTC 1 cut(s) 335
Bsp120I GGGCCC 1 cut(s) 166
Bsp1286I GDGCHC 2 cut(s) 170, 270
Bsp143I GATC 4 cut(s) 57, 67, 103, 533
BspACI CCGC 4 cut(s) 120, 345, 397, 575
BspANI GGCC 4 cut(s) 87, 168, 369, 396
BspFNI CGCG 1 cut(s) 399
BspLI GGNNCC 2 cut(s) 93, 168
BspPI GGATC 2 cut(s) 65, 528
BspQI GCTCTTC 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 347
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 4 cut(s) 57, 67, 103, 533
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 3 cut(s) 160, 272, 571
Bst6I CTCTTC 2 cut(s) 29, 258
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 4 cut(s) 46, 138, 383, 387
BstDEI CTNAG 1 cut(s) 250
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 1 cut(s) 475
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 4 cut(s) 60, 70, 106, 536
BstMAI GTCTC 2 cut(s) 335, 414
BstMBI GATC 4 cut(s) 57, 67, 103, 533
BstMWI GCNNNNNNNGC 2 cut(s) 152, 366
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 170
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 2 cut(s) 164, 565
BsuRI GGCC 4 cut(s) 87, 168, 369, 396
BtgI CCRYGG 1 cut(s) 570
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 475
Cac8I GCNNGC 4 cut(s) 46, 138, 383, 387
Cfr13I GGNCC 3 cut(s) 166, 167, 514
CseI GACGC 2 cut(s) 5, 513
CspCI CAANNNNNGTGG 2 cut(s) 144, 179
CviAII CATG 5 cut(s) 49, 137, 235, 283, 521
DdeI CTNAG 1 cut(s) 250
DpnI GATC 4 cut(s) 59, 69, 105, 535
DpnII GATC 4 cut(s) 57, 67, 103, 533
DrdI GACNNNNNNGTC 1 cut(s) 286
DseDI GACNNNNNNGTC 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 29, 258
EarI CTCTTC 2 cut(s) 29, 258
EciI GGCGGA 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 375
Eco72I CACGTG 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 170
FaeI CATG 5 cut(s) 52, 140, 238, 286, 524
FatI CATG 5 cut(s) 48, 136, 234, 282, 520
Fnu4HI GCNGC 4 cut(s) 153, 397, 576, 579
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 4 cut(s) 153, 397, 576, 579
FspBI CTAG 1 cut(s) 440
GluI GCNGC 4 cut(s) 153, 397, 576, 579
HaeIII GGCC 4 cut(s) 87, 168, 369, 396
HapII CCGG 1 cut(s) 555
HgaI GACGC 2 cut(s) 5, 513
Hin1II CATG 5 cut(s) 52, 140, 238, 286, 524
HpaII CCGG 1 cut(s) 555
HphI GGTGA 2 cut(s) 52, 282
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 30, 72
Hpy188III TCNNGA 4 cut(s) 277, 329, 440, 531
Hpy8I GTNNAC 1 cut(s) 20
Hpy99I CGWCG 1 cut(s) 529
HpyAV CCTTC 3 cut(s) 77, 306, 365
HpyCH4III ACNGT 3 cut(s) 160, 272, 571
HpyCH4IV ACGT 2 cut(s) 78, 362
HpyCH4V TGCA 4 cut(s) 140, 146, 152, 375
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 366
HpyF3I CTNAG 1 cut(s) 250
HpySE526I ACGT 2 cut(s) 78, 362
Hsp92II CATG 5 cut(s) 52, 140, 238, 286, 524
Kzo9I GATC 4 cut(s) 57, 67, 103, 533
LguI GCTCTTC 1 cut(s) 258
Lsp1109I GCAGC 2 cut(s) 164, 565
MaeI CTAG 1 cut(s) 440
MaeII ACGT 2 cut(s) 78, 362
MaeIII GTNAC 2 cut(s) 288, 528
MalI GATC 4 cut(s) 59, 69, 105, 535
MbiI CCGCTC 1 cut(s) 347
MboI GATC 4 cut(s) 57, 67, 103, 533
MboII GAAGA 3 cut(s) 46, 275, 321
MhlI GDGCHC 2 cut(s) 170, 270
MluCI AATT 2 cut(s) 205, 211
MnlI CCTC 8 cut(s) 36, 127, 380, 505, 511, 550, 559, 562
MseI TTAA 2 cut(s) 192, 462
MslI CAYNNNNRTG 1 cut(s) 407
MspI CCGG 1 cut(s) 555
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 2 cut(s) 152, 366
NdeII GATC 4 cut(s) 57, 67, 103, 533
NlaIII CATG 5 cut(s) 52, 140, 238, 286, 524
NlaIV GGNNCC 2 cut(s) 93, 168
NmeAIII GCCGAG 1 cut(s) 299
NmuCI GTSAC 2 cut(s) 288, 528
NspI RCATGY 1 cut(s) 140
PaeI GCATGC 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 258
PflMI CCANNNNNTGG 1 cut(s) 571
PkrI GCNGC 4 cut(s) 154, 398, 577, 580
PmaCI CACGTG 1 cut(s) 363
PmlI CACGTG 1 cut(s) 363
Ppu21I YACGTR 1 cut(s) 363
PsiI TTATAA 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 87
PspCI CACGTG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 93, 168
PspOMI GGGCCC 1 cut(s) 166
PspPI GGNCC 3 cut(s) 166, 167, 514
RseI CAYNNNNRTG 1 cut(s) 407
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 2 cut(s) 192, 462
SatI GCNGC 4 cut(s) 153, 397, 576, 579
Sau3AI GATC 4 cut(s) 57, 67, 103, 533
Sau96I GGNCC 3 cut(s) 166, 167, 514
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 2 cut(s) 170, 270
SetI ASST 8 cut(s) 81, 181, 251, 365, 387, 496, 542, 551
SinI GGWCC 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
SphI GCATGC 1 cut(s) 140
Sse9I AATT 2 cut(s) 205, 211
SsiI CCGC 4 cut(s) 120, 345, 397, 575
SspMI CTAG 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 3 cut(s) 160, 272, 571
TaiI ACGT 2 cut(s) 81, 365
TasI AATT 2 cut(s) 205, 211
TauI GCSGC 2 cut(s) 399, 578
Tru1I TTAA 2 cut(s) 192, 462
Tru9I TTAA 2 cut(s) 192, 462
TseFI GTSAC 2 cut(s) 288, 528
TseI GCWGC 2 cut(s) 152, 578
Tsp45I GTSAC 2 cut(s) 288, 528
TspDTI ATGAA 4 cut(s) 47, 245, 251, 276
TspGWI ACGGA 1 cut(s) 89
Van91I CCANNNNNTGG 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 514
XbaI TCTAGA 1 cut(s) 439
XceI RCATGY 1 cut(s) 140
XspI CTAG 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.