MD00G1097400.v1.1

Four repeated domains in the Fasciclin I family of proteins, present in many other contexts.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005034
Physical Location & Seq
Forward (+)
20398474 .. 20399310
837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1097400.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCGCCGAACCACCACTCATTTCTTCTAATCTTCTTTCTCACCACTCTGTTCTCAGCCATCAACCCAATCGCCACCGTCACTGCCTCTCCCTCACCGCCACCGCCACCGCCACAAACCCCATCTCCATCTCCATCACCATCACCATCATTAACACCACCATCCCCACCGCCATTATCTCCGCCACCGCCTCCTCCGCATTCACGGCCATCCCCAAGCGCGACGCCCCAACAACTCAACAACATAATCGACGCCCTAATCGGAGCTGGCGACTTCGGGAACTGGGTCAACATCATCACCGGCGCAAACCCACTAGCCCTCCCTTTGAGCGCCACCCTCTTCATCCCGCAAGACAACGCTTTGAACAGCCTCCCCACCACCGACCCCTTCATGTTCCCCTACCACGTCGTCCCGCAGCGCCTCAGCTTTGCCGACCTCCAGCTCTTCAAGCCCGGATCCCGCCTCCCTACTCTGCTCCCCGGCAAGTCCATCCTCATCACCAACACCTCCCCCTCCAATTTCACCCTCGACGACGCTCCGATCACTCAGCCCGACCTCTACGTCACCGCCACGATGGCGGTCCACGGTGTCGGAACTATTCTTCAGTACTCCGTTTACGGCGACGGGCTCAACCTCTTGCCCAAGCCCAATTCCCAGCAGGGGCAGCAGCGGCATAATCCTCCGCCGTCATCGCCGCAGGGGCTGTTTCTGCCAACCGGGGAAGCCATCGGCGCTGGGTGGAAGTCTGATGCGGCGCCGCCGTGCAGCTGTGTCGAGTTTCCGGTGGGGTGGTTCTTGGTTGCTTGTGTGGTTTTGGGGTTCAGGATTCACAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fasciclin

Protein Analysis

279

Amino Acids

29.43

Weight (kDa)

6.34

Isoelectric Point (pI)

69.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 109 - 201 5.1e-06 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 560
AccB1I GGYRCC 2 cut(s) 4, 754
AccII CGCG 1 cut(s) 221
AclWI GGATC 2 cut(s) 450, 463
AcoI YGGCCR 1 cut(s) 206
AcuI CTGAAG 1 cut(s) 587
AcyI GRCGYC 4 cut(s) 5, 224, 252, 755
AfaI GTAC 1 cut(s) 608
AfiI CCNNNNNNNGG 1 cut(s) 660
AgsI TTSAA 2 cut(s) 364, 448
AjiI CACGTC 1 cut(s) 406
AluBI AGCT 4 cut(s) 266, 426, 442, 768
AluI AGCT 4 cut(s) 266, 426, 442, 768
AlwI GGATC 2 cut(s) 450, 463
AlwNI CAGNNNCTG 1 cut(s) 703
AoxI GGCC 1 cut(s) 206
ApeKI GCWGC 4 cut(s) 415, 664, 667, 765
AspLEI GCGC 7 cut(s) 7, 221, 305, 332, 420, 734, 757
AspS9I GGNCC 1 cut(s) 580
AsuC2I CCSGG 3 cut(s) 453, 480, 718
AsuHPI GGTGA 8 cut(s) 34, 87, 129, 135, 289, 490, 514, 556
AvaII GGWCC 1 cut(s) 580
BamHI GGATCC 1 cut(s) 455
BanI GGYRCC 2 cut(s) 4, 754
BanII GRGCYC 1 cut(s) 630
BbvCI CCTCAGC 1 cut(s) 422
BbvI GCAGC 4 cut(s) 427, 676, 679, 777
BceAI ACGGC 4 cut(s) 221, 634, 670, 745
BcgI CGANNNNNNTGC 2 cut(s) 754, 788
BcnI CCSGG 3 cut(s) 453, 480, 718
BfaI CTAG 1 cut(s) 314
BfoI RGCGCY 5 cut(s) 8, 333, 421, 735, 758
BglI GCCNNNNNGGC 2 cut(s) 575, 700
BisI GCNGC 8 cut(s) 416, 665, 668, 671, 695, 753, 758, 766
BlsI GCNGC 8 cut(s) 417, 666, 669, 672, 696, 754, 759, 767
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 3 cut(s) 453, 480, 718
Bme18I GGWCC 1 cut(s) 580
BmgBI CACGTC 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 3 cut(s) 6, 457, 756
BmrFI CCNGG 3 cut(s) 453, 480, 718
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 739
BmuI ACTGGG 1 cut(s) 292
BpmI CTGGAG 1 cut(s) 422
Bpu10I CCTNAGC 1 cut(s) 422
BpuMI CCSGG 3 cut(s) 453, 480, 718
BsaHI GRCGYC 4 cut(s) 5, 224, 252, 755
BsaJI CCNNGG 3 cut(s) 478, 583, 717
BsaWI WCCGGW 1 cut(s) 781
BsaXI ACNNNNNCTCC 6 cut(s) 73, 103, 109, 139, 303, 333
Bsc4I CCNNNNNNNGG 1 cut(s) 660
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 1 cut(s) 287
BseDI CCNNGG 3 cut(s) 478, 583, 717
BseGI GGATG 4 cut(s) 161, 209, 342, 489
BseLI CCNNNNNNNGG 1 cut(s) 660
BseMII CTCAG 3 cut(s) 69, 436, 560
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 4 cut(s) 427, 676, 679, 777
BseYI CCCAGC 2 cut(s) 654, 734
BsgI GTGCAG 1 cut(s) 784
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 1 cut(s) 208
BshNI GGYRCC 2 cut(s) 4, 754
BsiSI CCGG 5 cut(s) 300, 453, 480, 717, 782
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 660
BsmFI GGGAC 1 cut(s) 395
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 630
Bsp143I GATC 2 cut(s) 455, 540
BspANI GGCC 1 cut(s) 208
BspCNI CTCAG 3 cut(s) 68, 435, 559
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 3 cut(s) 6, 457, 756
BspPI GGATC 2 cut(s) 450, 463
BspQI GCTCTTC 1 cut(s) 449
BspT107I GGYRCC 2 cut(s) 4, 754
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 1 cut(s) 287
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 3 cut(s) 478, 583, 717
BssMI GATC 2 cut(s) 455, 540
BssNI GRCGYC 4 cut(s) 5, 224, 252, 755
Bst4CI ACNGT 2 cut(s) 79, 587
Bst6I CTCTTC 2 cut(s) 344, 449
BstACI GRCGYC 4 cut(s) 5, 224, 252, 755
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 3 cut(s) 55, 422, 546
BstDSI CCRYGG 1 cut(s) 583
BstF5I GGATG 4 cut(s) 161, 209, 342, 489
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 5 cut(s) 8, 333, 421, 735, 758
BstHHI GCGC 7 cut(s) 7, 221, 305, 332, 420, 734, 757
BstKTI GATC 2 cut(s) 458, 543
BstMBI GATC 2 cut(s) 455, 540
BstSCI CCNGG 3 cut(s) 451, 478, 716
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 4 cut(s) 427, 676, 679, 777
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BsuRI GGCC 1 cut(s) 208
BtgI CCRYGG 1 cut(s) 583
BtgZI GCGATG 1 cut(s) 675
BtrI CACGTC 1 cut(s) 406
BtsCI GGATG 4 cut(s) 161, 209, 342, 489
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 268
CaiI CAGNNNCTG 1 cut(s) 703
CfoI GCGC 7 cut(s) 7, 221, 305, 332, 420, 734, 757
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 580
CseI GACGC 3 cut(s) 232, 260, 542
Csp6I GTAC 1 cut(s) 607
CviAII CATG 1 cut(s) 391
CviQI GTAC 1 cut(s) 607
DdeI CTNAG 3 cut(s) 55, 422, 546
DinI GGCGCC 2 cut(s) 6, 756
DpnI GATC 2 cut(s) 457, 542
DpnII GATC 2 cut(s) 455, 540
DrdI GACNNNNNNGTC 1 cut(s) 560
DseDI GACNNNNNNGTC 1 cut(s) 560
EaeI YGGCCR 1 cut(s) 206
Eam1104I CTCTTC 2 cut(s) 344, 449
EarI CTCTTC 2 cut(s) 344, 449
EciI GGCGGA 2 cut(s) 171, 672
Eco24I GRGCYC 1 cut(s) 630
Eco47I GGWCC 1 cut(s) 580
Eco57I CTGAAG 1 cut(s) 587
EcoT38I GRGCYC 1 cut(s) 630
EgeI GGCGCC 2 cut(s) 6, 756
EheI GGCGCC 2 cut(s) 6, 756
FaeI CATG 1 cut(s) 394
FaiI YATR 3 cut(s) 245, 392, 675
FaqI GGGAC 1 cut(s) 395
FatI CATG 1 cut(s) 390
FauI CCCGC 3 cut(s) 354, 420, 467
Fnu4HI GCNGC 8 cut(s) 416, 665, 668, 671, 695, 753, 758, 766
FokI GGATG 4 cut(s) 148, 196, 329, 476
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 8 cut(s) 416, 665, 668, 671, 695, 753, 758, 766
FspBI CTAG 1 cut(s) 314
GlaI GCGC 7 cut(s) 6, 220, 304, 331, 419, 733, 756
GluI GCNGC 8 cut(s) 416, 665, 668, 671, 695, 753, 758, 766
GsaI CCCAGC 2 cut(s) 658, 738
GsuI CTGGAG 1 cut(s) 422
HaeII RGCGCY 5 cut(s) 8, 333, 421, 735, 758
HaeIII GGCC 1 cut(s) 208
HapII CCGG 5 cut(s) 300, 453, 480, 717, 782
HgaI GACGC 3 cut(s) 232, 260, 542
HhaI GCGC 7 cut(s) 7, 221, 305, 332, 420, 734, 757
Hin1I GRCGYC 4 cut(s) 5, 224, 252, 755
Hin1II CATG 1 cut(s) 394
Hin6I GCGC 7 cut(s) 5, 219, 303, 330, 418, 732, 755
HinP1I GCGC 7 cut(s) 5, 219, 303, 330, 418, 732, 755
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 1 cut(s) 826
HpaII CCGG 5 cut(s) 300, 453, 480, 717, 782
HphI GGTGA 8 cut(s) 34, 87, 129, 135, 289, 490, 514, 556
Hpy166II GTNNAC 3 cut(s) 289, 583, 616
Hpy188I TCNGA 4 cut(s) 263, 540, 593, 748
Hpy188III TCNNGA 2 cut(s) 277, 823
Hpy8I GTNNAC 3 cut(s) 289, 583, 616
Hpy99I CGWCG 6 cut(s) 226, 254, 410, 533, 536, 626
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 2 cut(s) 79, 587
HpyCH4IV ACGT 2 cut(s) 405, 561
HpyCH4V TGCA 1 cut(s) 765
HpyF3I CTNAG 3 cut(s) 55, 422, 546
HpySE526I ACGT 2 cut(s) 405, 561
Hsp92I GRCGYC 4 cut(s) 5, 224, 252, 755
Hsp92II CATG 1 cut(s) 394
HspAI GCGC 7 cut(s) 5, 219, 303, 330, 418, 732, 755
KasI GGCGCC 2 cut(s) 4, 754
Kzo9I GATC 2 cut(s) 455, 540
LguI GCTCTTC 1 cut(s) 449
LmnI GCTCC 3 cut(s) 263, 480, 541
Lsp1109I GCAGC 4 cut(s) 427, 676, 679, 777
LweI GCATC 1 cut(s) 739
MaeI CTAG 1 cut(s) 314
MaeII ACGT 2 cut(s) 405, 561
MaeIII GTNAC 2 cut(s) 79, 562
MalI GATC 2 cut(s) 457, 542
MboI GATC 2 cut(s) 455, 540
MboII GAAGA 5 cut(s) 17, 25, 331, 436, 593
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 1 cut(s) 630
MluCI AATT 2 cut(s) 517, 649
Mly113I GGCGCC 2 cut(s) 5, 755
MmeI TCCRAC 1 cut(s) 571
MseI TTAA 1 cut(s) 152
MspA1I CMGCKG 2 cut(s) 670, 768
MspI CCGG 5 cut(s) 300, 453, 480, 717, 782
MspR9I CCNGG 3 cut(s) 453, 480, 718
Mva1269I GAATGC 1 cut(s) 199
MvnI CGCG 1 cut(s) 221
NarI GGCGCC 2 cut(s) 5, 755
NciI CCSGG 3 cut(s) 453, 480, 718
NdeII GATC 2 cut(s) 455, 540
NlaIII CATG 1 cut(s) 394
NlaIV GGNNCC 3 cut(s) 6, 457, 756
NmuCI GTSAC 2 cut(s) 79, 562
PciSI GCTCTTC 1 cut(s) 449
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 826
PkrI GCNGC 8 cut(s) 417, 666, 669, 672, 696, 754, 759, 767
PluTI GGCGCC 2 cut(s) 8, 758
PspFI CCCAGC 2 cut(s) 654, 734
PspN4I GGNNCC 3 cut(s) 6, 457, 756
PspPI GGNCC 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 703
PsuI RGATCY 1 cut(s) 455
PvuII CAGCTG 1 cut(s) 768
RsaI GTAC 1 cut(s) 608
RsaNI GTAC 1 cut(s) 607
SapI GCTCTTC 1 cut(s) 449
SaqAI TTAA 1 cut(s) 152
SatI GCNGC 8 cut(s) 416, 665, 668, 671, 695, 753, 758, 766
Sau3AI GATC 2 cut(s) 455, 540
Sau96I GGNCC 1 cut(s) 580
ScaI AGTACT 1 cut(s) 608
ScrFI CCNGG 3 cut(s) 453, 480, 718
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 1 cut(s) 739
SfoI GGCGCC 2 cut(s) 6, 756
SgrAI CRCCGGYG 1 cut(s) 299
SinI GGWCC 1 cut(s) 580
Sse9I AATT 2 cut(s) 517, 649
SspDI GGCGCC 2 cut(s) 4, 754
SspMI CTAG 1 cut(s) 314
StyD4I CCNGG 3 cut(s) 451, 478, 716
TaaI ACNGT 2 cut(s) 79, 587
TaiI ACGT 2 cut(s) 408, 564
TaqI TCGA 3 cut(s) 249, 528, 774
TasI AATT 2 cut(s) 517, 649
TatI WGTACW 1 cut(s) 606
TauI GCSGC 4 cut(s) 673, 697, 755, 760
TfiI GAWTC 1 cut(s) 826
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 2 cut(s) 79, 562
TseI GCWGC 4 cut(s) 415, 664, 667, 765
Tsp45I GTSAC 2 cut(s) 79, 562
TspDTI ATGAA 2 cut(s) 331, 379
TspGWI ACGGA 1 cut(s) 601
TspRI CASTG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 580
XspI CTAG 1 cut(s) 314
ZrmI AGTACT 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.