MD01G1036800.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003904
Physical Location & Seq
Reverse (-)
12366522 .. 12367110
589 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1036800.v1.1.491

Sequence Viewer

Length: 543 bp
ATGTGGAATCGCGAGAACGACGTCGTCCACAATTCTGGCAGTAATCGCGAGTGCGAACGCAAGCAAGGGGTTCCATGGATGAAGGAAGAGCACAAGCTTTTCTTGCTTGGATTGCAGAAAGTAGGGAAAGGAGACTGGAGAGGGATCTCAAGAAACTTCGTGAAGACTCGCACCCCGACTCAGGTTGCCAGCCACGCACAGAAGTACTTTCTGCGCCGGAACAACCACAATCGTCGGCGCCGCACATCTAGCCTATTCGACATCACCACCGAAACGGCCTCTCCAACTCCAACGGATGAAGAGCTAGTACATCTTCAAGATAACGCATCTCAGTCGCATCCTTTGCCTCATCCGCCACTATCGAACCCTCGCAACGCCAGTGATTTTCCAATGACTGTAGGTCTAGCTGCCTTGCCAGTTCCCATTGAGAATCCTATGGAAAATCTAGCTCTACGACAGGCAAATCACGAGAACAGTGCCTTGGCTGAGCTCGTCCATCCAGTTGCCCTTCAATCGTCCCATCACCATCACCATTTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

181

Amino Acids

20.51

Weight (kDa)

9.3

Isoelectric Point (pI)

63.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 24 - 70 6.1e-10 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 237
AccII CGCG 2 cut(s) 12, 48
AciI CCGC 2 cut(s) 241, 353
AclWI GGATC 1 cut(s) 152
AcyI GRCGYC 2 cut(s) 21, 238
AfaI GTAC 2 cut(s) 206, 309
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 2 cut(s) 317, 512
AhdI GACNNNNNGTC 1 cut(s) 399
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AluBI AGCT 5 cut(s) 97, 304, 407, 449, 490
AluI AGCT 5 cut(s) 97, 304, 407, 449, 490
Alw21I GWGCWC 2 cut(s) 93, 492
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 1 cut(s) 152
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 1 cut(s) 407
AspLEI GCGC 2 cut(s) 216, 240
AsuHPI GGTGA 3 cut(s) 256, 515, 521
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 492
BarI GAAGNNNNNNTAC 2 cut(s) 291, 323
BauI CACGAG 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 170
Bbv12I GWGCWC 2 cut(s) 93, 492
BbvI GCAGC 1 cut(s) 394
BccI CCATC 3 cut(s) 504, 528, 534
BceAI ACGGC 1 cut(s) 291
BcgI CGANNNNNNTGC 4 cut(s) 315, 349, 458, 492
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 4 cut(s) 249, 305, 404, 446
BfmI CTRYAG 1 cut(s) 396
BfoI RGCGCY 1 cut(s) 241
BisI GCNGC 2 cut(s) 241, 408
BlpI GCTNAGC 1 cut(s) 486
BlsI GCNGC 2 cut(s) 242, 409
BmcAI AGTACT 1 cut(s) 206
BmeRI GACNNNNNGTC 1 cut(s) 399
BmiI GGNNCC 2 cut(s) 72, 239
BmsI GCATC 2 cut(s) 335, 346
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 1 cut(s) 157
Bpu1102I GCTNAGC 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 133
BsaHI GRCGYC 2 cut(s) 21, 238
BsaJI CCNNGG 2 cut(s) 74, 480
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse1I ACTGG 4 cut(s) 140, 378, 416, 500
BseDI CCNNGG 2 cut(s) 74, 480
BseGI GGATG 5 cut(s) 84, 301, 337, 349, 496
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMII CTCAG 3 cut(s) 194, 344, 477
BseNI ACTGG 4 cut(s) 140, 378, 416, 500
BseXI GCAGC 1 cut(s) 394
Bsh1236I CGCG 2 cut(s) 12, 48
BshFI GGCC 1 cut(s) 278
BshNI GGYRCC 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 93, 492
BsiSI CCGG 1 cut(s) 217
BslFI GGGAC 1 cut(s) 502
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 502
BsnI GGCC 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 93, 492
Bsp143I GATC 1 cut(s) 144
Bsp1720I GCTNAGC 1 cut(s) 486
Bsp19I CCATGG 1 cut(s) 74
Bsp68I TCGCGA 2 cut(s) 12, 48
BspACI CCGC 2 cut(s) 241, 353
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 3 cut(s) 193, 343, 478
BspFNI CGCG 2 cut(s) 12, 48
BspLI GGNNCC 2 cut(s) 72, 239
BspPI GGATC 1 cut(s) 152
BspQI GCTCTTC 2 cut(s) 81, 294
BspT107I GGYRCC 1 cut(s) 237
BsrI ACTGG 4 cut(s) 140, 378, 416, 500
BssECI CCNNGG 2 cut(s) 74, 480
BssMI GATC 1 cut(s) 144
BssNI GRCGYC 2 cut(s) 21, 238
BssSI CACGAG 1 cut(s) 467
BssT1I CCWWGG 2 cut(s) 74, 480
Bst2BI CACGAG 1 cut(s) 467
Bst4CI ACNGT 2 cut(s) 397, 476
Bst6I CTCTTC 2 cut(s) 81, 294
BstACI GRCGYC 2 cut(s) 21, 238
BstAPI GCANNNNNTGC 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 62, 190
BstDEI CTNAG 3 cut(s) 180, 330, 486
BstDSI CCRYGG 1 cut(s) 74
BstF5I GGATG 5 cut(s) 84, 301, 337, 349, 496
BstFNI CGCG 2 cut(s) 12, 48
BstH2I RGCGCY 1 cut(s) 241
BstHHI GCGC 2 cut(s) 216, 240
BstKTI GATC 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 1 cut(s) 144
BstMWI GCNNNNNNNGC 7 cut(s) 45, 103, 112, 194, 249, 343, 352
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 2 cut(s) 12, 48
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 144
BstXI CCANNNNNNTGG 1 cut(s) 35
BstYI RGATCY 1 cut(s) 144
BsuRI GGCC 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 74
BtsCI GGATG 5 cut(s) 84, 301, 337, 349, 496
BtsIMutI CAGTG 2 cut(s) 385, 481
BtuMI TCGCGA 2 cut(s) 12, 48
Cac8I GCNNGC 2 cut(s) 62, 190
CfoI GCGC 2 cut(s) 216, 240
Csp6I GTAC 2 cut(s) 205, 308
CviAII CATG 1 cut(s) 75
CviJI RGCY 9 cut(s) 97, 192, 252, 278, 304, 407, 449, 485, 490
CviKI_1 RGCY 9 cut(s) 97, 192, 252, 278, 304, 407, 449, 485, 490
CviQI GTAC 2 cut(s) 205, 308
DdeI CTNAG 3 cut(s) 180, 330, 486
DinI GGCGCC 1 cut(s) 239
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
DriI GACNNNNNGTC 1 cut(s) 399
Eam1104I CTCTTC 2 cut(s) 81, 294
Eam1105I GACNNNNNGTC 1 cut(s) 399
EarI CTCTTC 2 cut(s) 81, 294
EciI GGCGGA 1 cut(s) 342
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 2 cut(s) 74, 480
Eco24I GRGCYC 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 490
EcoICRI GAGCTC 1 cut(s) 490
EcoT14I CCWWGG 2 cut(s) 74, 480
EcoT38I GRGCYC 1 cut(s) 492
EgeI GGCGCC 1 cut(s) 239
EheI GGCGCC 1 cut(s) 239
ErhI CCWWGG 2 cut(s) 74, 480
FaeI CATG 1 cut(s) 78
FaiI YATR 2 cut(s) 76, 437
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 1 cut(s) 502
FatI CATG 1 cut(s) 74
Fnu4HI GCNGC 2 cut(s) 241, 408
FokI GGATG 5 cut(s) 91, 308, 324, 336, 483
FriOI GRGCYC 1 cut(s) 492
Fsp4HI GCNGC 2 cut(s) 241, 408
FspBI CTAG 4 cut(s) 249, 305, 404, 446
GlaI GCGC 2 cut(s) 215, 239
GluI GCNGC 2 cut(s) 241, 408
GsuI CTGGAG 1 cut(s) 157
HaeII RGCGCY 1 cut(s) 241
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 217
HhaI GCGC 2 cut(s) 216, 240
Hin1I GRCGYC 2 cut(s) 21, 238
Hin1II CATG 1 cut(s) 78
Hin6I GCGC 2 cut(s) 214, 238
HinP1I GCGC 2 cut(s) 214, 238
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 4 cut(s) 7, 166, 178, 430
HpaII CCGG 1 cut(s) 217
HphI GGTGA 3 cut(s) 256, 515, 521
Hpy166II GTNNAC 1 cut(s) 28
Hpy188III TCNNGA 6 cut(s) 11, 47, 150, 160, 317, 467
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 3 cut(s) 23, 26, 237
HpyAV CCTTC 2 cut(s) 76, 518
HpyCH4III ACNGT 2 cut(s) 397, 476
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 7 cut(s) 45, 103, 112, 194, 249, 343, 352
HpyF3I CTNAG 3 cut(s) 180, 330, 486
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 2 cut(s) 21, 238
Hsp92II CATG 1 cut(s) 78
HspAI GCGC 2 cut(s) 214, 238
KasI GGCGCC 1 cut(s) 237
Kzo9I GATC 1 cut(s) 144
LguI GCTCTTC 2 cut(s) 81, 294
LpnPI CCDG 9 cut(s) 21, 121, 167, 202, 230, 391, 429, 443, 513
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 2 cut(s) 335, 346
MaeI CTAG 4 cut(s) 249, 305, 404, 446
MaeII ACGT 1 cut(s) 21
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MboII GAAGA 4 cut(s) 98, 175, 305, 311
MflI RGATCY 1 cut(s) 144
MhlI GDGCHC 2 cut(s) 93, 492
MluCI AATT 1 cut(s) 31
Mly113I GGCGCC 1 cut(s) 238
MlyI GAGTC 2 cut(s) 160, 172
MmeI TCCRAC 2 cut(s) 308, 314
MnlI CCTC 4 cut(s) 134, 289, 357, 378
MspI CCGG 1 cut(s) 217
MvnI CGCG 2 cut(s) 12, 48
MwoI GCNNNNNNNGC 7 cut(s) 45, 103, 112, 194, 249, 343, 352
NarI GGCGCC 1 cut(s) 238
NcoI CCATGG 1 cut(s) 74
NdeII GATC 1 cut(s) 144
NlaIII CATG 1 cut(s) 78
NlaIV GGNNCC 2 cut(s) 72, 239
NruI TCGCGA 2 cut(s) 12, 48
PciSI GCTCTTC 2 cut(s) 81, 294
PfeI GAWTC 2 cut(s) 7, 430
PflFI GACNNNGTC 1 cut(s) 23
PkrI GCNGC 2 cut(s) 242, 409
PleI GAGTC 2 cut(s) 160, 172
PluTI GGCGCC 1 cut(s) 241
PpsI GAGTC 2 cut(s) 160, 172
Psp124BI GAGCTC 1 cut(s) 492
PspN4I GGNNCC 2 cut(s) 72, 239
PsuI RGATCY 1 cut(s) 144
PsyI GACNNNGTC 1 cut(s) 23
RruI TCGCGA 2 cut(s) 12, 48
RsaI GTAC 2 cut(s) 206, 309
RsaNI GTAC 2 cut(s) 205, 308
SacI GAGCTC 1 cut(s) 492
SapI GCTCTTC 2 cut(s) 81, 294
SatI GCNGC 2 cut(s) 241, 408
Sau3AI GATC 1 cut(s) 144
ScaI AGTACT 1 cut(s) 206
SchI GAGTC 2 cut(s) 160, 172
SduI GDGCHC 2 cut(s) 93, 492
SetI ASST 8 cut(s) 24, 99, 186, 306, 403, 409, 451, 492
SfaNI GCATC 2 cut(s) 335, 346
SfcI CTRYAG 1 cut(s) 396
SfoI GGCGCC 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 1 cut(s) 31
SsiI CCGC 2 cut(s) 241, 353
SspDI GGCGCC 1 cut(s) 237
SspMI CTAG 4 cut(s) 249, 305, 404, 446
SstI GAGCTC 1 cut(s) 492
StyI CCWWGG 2 cut(s) 74, 480
TaaI ACNGT 2 cut(s) 397, 476
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 258, 362
TasI AATT 1 cut(s) 31
TatI WGTACW 2 cut(s) 204, 307
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 2 cut(s) 7, 430
TscAI CASTG 2 cut(s) 385, 481
TseI GCWGC 1 cut(s) 407
TspDTI ATGAA 2 cut(s) 95, 312
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 2 cut(s) 385, 481
Tth111I GACNNNGTC 1 cut(s) 23
XspI CTAG 4 cut(s) 249, 305, 404, 446
ZraI GACGTC 1 cut(s) 22
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.