MD06G1183300.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003904
Physical Location & Seq
Forward (+)
32173950 .. 32176088
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1183300.v1.1.491

Sequence Viewer

Length: 903 bp
ATGTCGTCGGGCACGTGCTCCACCGTCGAGCCCGCGGGCGCCGGAGAATTCATGCTGTTCGGCGTGCGCGTGGTGGTCGATTCCATGAGGAAGAGCGTCAGTTTGAACAATCTCTCGCAGTACGAGCAGCCTCAGGAGGCCGCCTCCAACAACGGAAATAACGGCACCGCCGCCGGAAAGGATGACGCGGCGCCCGGTTACGCCTCCGAGAACGACGTCGTCCACAATTCTGGCGGGAATCGCGAGCGCGAACGCAAGCGAGGGGTTCCATGGACGGAGGAAGAGCACAAGCTTTTCTTGCTTGGATTGCAGAAAGTAGGGAAAGGAGATTGGAGAGGGATCTCAAGAAACTTCGTGAAGACTCGCACCCCGACTCAGGTTGCCAGCCACGCACAGAAGTACTTTCTGCGCCGGAACAACCACAATCGCCGGCGCCGCAGATCTAGCCTCTTCGACATCACCACCGAAACGGTCTCTCCAACTCCAATGGACGAAGAGCAAGTACATCTTCAAGATAACGCATCTCAGTCGCATCCATTGCCTCCTCCGCCACTATCCGACCCTCGCAACGCCAGTGGATTTCCAGTGGTGCCAGATTTTCCAATGACTGTAGGTCCAGCTGCCTTGCCAGTTCCCATTGAGAATCCTACGGAAAATCTAGCTCTACGACAAGCAAATCACGAGAATAGTACCTTGCCTAAGCTCGTCCATCCAGCTGCCCTTCACTCGGCCCCTCATGCCGCTGCAATGTCTGATCTAAACTTGAACTCAACAATGGACCCATCAACTCTCACTCTCAACCTCTCCTTGTCAATGGAGTCGAGGGAACCGTCCTCAAGGCATTCGGCTTTCCAGACAATGTCAGGATTCAGCAATGGGGATAGCATGATCAGCGTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

301

Amino Acids

32.66

Weight (kDa)

6.6

Isoelectric Point (pI)

59.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 89 - 135 1.7e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
AccB1I GGYRCC 5 cut(s) 38, 164, 190, 432, 589
AccII CGCG 5 cut(s) 35, 69, 188, 243, 249
AclWI GGATC 1 cut(s) 347
AcsI RAATTY 1 cut(s) 47
AcvI CACGTG 1 cut(s) 15
AcyI GRCGYC 4 cut(s) 39, 191, 216, 433
AfaI GTAC 4 cut(s) 122, 401, 504, 691
AfiI CCNNNNNNNGG 2 cut(s) 376, 727
AgsI TTSAA 3 cut(s) 106, 512, 766
AhdI GACNNNNNGTC 1 cut(s) 612
AluBI AGCT 5 cut(s) 292, 620, 662, 703, 716
AluI AGCT 5 cut(s) 292, 620, 662, 703, 716
Alw21I GWGCWC 2 cut(s) 20, 288
Alw26I GTCTC 1 cut(s) 478
AlwI GGATC 1 cut(s) 347
AoxI GGCC 2 cut(s) 138, 729
ApeKI GCWGC 4 cut(s) 127, 620, 716, 743
ApoI RAATTY 1 cut(s) 47
AspLEI GCGC 6 cut(s) 41, 69, 193, 249, 411, 435
AspS9I GGNCC 3 cut(s) 614, 730, 778
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 2 cut(s) 451, 889
AvaII GGWCC 2 cut(s) 614, 778
AxyI CCTNAGG 1 cut(s) 132
BaeGI GKGCMC 1 cut(s) 14
BanI GGYRCC 5 cut(s) 38, 164, 190, 432, 589
BanII GRGCYC 1 cut(s) 33
BarI GAAGNNNNNNTAC 2 cut(s) 486, 518
BauI CACGAG 1 cut(s) 680
BbrPI CACGTG 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 2 cut(s) 20, 288
BbvI GCAGC 4 cut(s) 139, 607, 703, 730
BccI CCATC 2 cut(s) 717, 790
BceAI ACGGC 1 cut(s) 178
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BclI TGATCA 1 cut(s) 888
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 478
BfaI CTAG 2 cut(s) 444, 659
BfmI CTRYAG 1 cut(s) 609
BfoI RGCGCY 3 cut(s) 42, 194, 436
BglII AGATCT 1 cut(s) 440
BisI GCNGC 9 cut(s) 128, 141, 171, 189, 436, 621, 717, 741, 744
BlsI GCNGC 9 cut(s) 129, 142, 172, 190, 437, 622, 718, 742, 745
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 2 cut(s) 614, 778
BmeRI GACNNNNNGTC 1 cut(s) 612
BmgT120I GGNCC 3 cut(s) 614, 730, 778
BmiI GGNNCC 9 cut(s) 40, 166, 192, 267, 434, 591, 732, 780, 828
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 2 cut(s) 530, 541
BpiI GAAGAC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 128, 160
Bpu10I CCTNAGC 1 cut(s) 699
BpuEI CTTGAG 2 cut(s) 328, 820
BpuMI CCSGG 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 15
BsaHI GRCGYC 4 cut(s) 39, 191, 216, 433
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 2 cut(s) 33, 269
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 727
Bse118I RCCGGY 1 cut(s) 429
Bse1I ACTGG 3 cut(s) 573, 584, 629
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 3 cut(s) 536, 753, 880
BseDI CCNNGG 2 cut(s) 33, 269
BseGI GGATG 3 cut(s) 187, 532, 709
BseLI CCNNNNNNNGG 2 cut(s) 376, 727
BseMI GCAATG 3 cut(s) 536, 753, 880
BseMII CTCAG 3 cut(s) 146, 389, 539
BseNI ACTGG 3 cut(s) 573, 584, 629
BseRI GAGGAG 1 cut(s) 534
BseSI GKGCMC 1 cut(s) 14
BseXI GCAGC 4 cut(s) 139, 607, 703, 730
Bsh1236I CGCG 5 cut(s) 35, 69, 188, 243, 249
BshFI GGCC 2 cut(s) 140, 731
BshNI GGYRCC 5 cut(s) 38, 164, 190, 432, 589
BsiHKAI GWGCWC 2 cut(s) 20, 288
BsiSI CCGG 5 cut(s) 42, 174, 195, 412, 430
BslI CCNNNNNNNGG 2 cut(s) 376, 727
BsmAI GTCTC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 841
BsnI GGCC 2 cut(s) 140, 731
Bso31I GGTCTC 1 cut(s) 478
Bsp1286I GDGCHC 4 cut(s) 14, 20, 33, 288
Bsp143I GATC 4 cut(s) 339, 440, 754, 888
Bsp19I CCATGG 1 cut(s) 269
Bsp68I TCGCGA 1 cut(s) 243
BspANI GGCC 2 cut(s) 140, 731
BspCNI CTCAG 3 cut(s) 145, 388, 538
BspFNI CGCG 5 cut(s) 35, 69, 188, 243, 249
BspLI GGNNCC 9 cut(s) 40, 166, 192, 267, 434, 591, 732, 780, 828
BspPI GGATC 1 cut(s) 347
BspQI GCTCTTC 3 cut(s) 86, 276, 489
BspT107I GGYRCC 5 cut(s) 38, 164, 190, 432, 589
BspTNI GGTCTC 1 cut(s) 478
BsrDI GCAATG 3 cut(s) 536, 753, 880
BsrFI RCCGGY 1 cut(s) 429
BsrI ACTGG 3 cut(s) 573, 584, 629
BssAI RCCGGY 1 cut(s) 429
BssECI CCNNGG 2 cut(s) 33, 269
BssMI GATC 4 cut(s) 339, 440, 754, 888
BssNI GRCGYC 4 cut(s) 39, 191, 216, 433
BssSI CACGAG 1 cut(s) 680
BssT1I CCWWGG 1 cut(s) 269
Bst2BI CACGAG 1 cut(s) 680
Bst4CI ACNGT 4 cut(s) 25, 472, 610, 831
Bst6I CTCTTC 4 cut(s) 86, 276, 455, 489
BstACI GRCGYC 4 cut(s) 39, 191, 216, 433
BstAPI GCANNNNNTGC 1 cut(s) 538
BstBAI YACGTR 1 cut(s) 15
BstC8I GCNNGC 7 cut(s) 33, 37, 65, 245, 257, 385, 431
BstDEI CTNAG 4 cut(s) 132, 375, 525, 699
BstDSI CCRYGG 2 cut(s) 33, 269
BstF5I GGATG 3 cut(s) 187, 532, 709
BstFNI CGCG 5 cut(s) 35, 69, 188, 243, 249
BstH2I RGCGCY 3 cut(s) 42, 194, 436
BstHHI GCGC 6 cut(s) 41, 69, 193, 249, 411, 435
BstKTI GATC 4 cut(s) 342, 443, 757, 891
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 4 cut(s) 339, 440, 754, 888
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 609
BstSLI GKGCMC 1 cut(s) 14
BstUI CGCG 5 cut(s) 35, 69, 188, 243, 249
BstV1I GCAGC 4 cut(s) 139, 607, 703, 730
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 2 cut(s) 339, 440
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 2 cut(s) 339, 440
Bsu36I CCTNAGG 1 cut(s) 132
BsuRI GGCC 2 cut(s) 140, 731
BtgI CCRYGG 2 cut(s) 33, 269
BtsCI GGATG 3 cut(s) 187, 532, 709
BtsIMutI CAGTG 2 cut(s) 580, 591
BtuMI TCGCGA 1 cut(s) 243
Cac8I GCNNGC 7 cut(s) 33, 37, 65, 245, 257, 385, 431
CfoI GCGC 6 cut(s) 41, 69, 193, 249, 411, 435
Cfr10I RCCGGY 1 cut(s) 429
Cfr13I GGNCC 3 cut(s) 614, 730, 778
Cfr42I CCGCGG 1 cut(s) 36
CseI GACGC 3 cut(s) 85, 194, 883
Csp6I GTAC 4 cut(s) 121, 400, 503, 690
CspCI CAANNNNNGTGG 2 cut(s) 556, 591
CviAII CATG 5 cut(s) 52, 85, 270, 737, 886
CviQI GTAC 4 cut(s) 121, 400, 503, 690
DdeI CTNAG 4 cut(s) 132, 375, 525, 699
DinI GGCGCC 3 cut(s) 40, 192, 434
DpnI GATC 4 cut(s) 341, 442, 756, 890
DpnII GATC 4 cut(s) 339, 440, 754, 888
DriI GACNNNNNGTC 1 cut(s) 612
Eam1104I CTCTTC 4 cut(s) 86, 276, 455, 489
Eam1105I GACNNNNNGTC 1 cut(s) 612
EarI CTCTTC 4 cut(s) 86, 276, 455, 489
EciI GGCGGA 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 614, 778
Eco72I CACGTG 1 cut(s) 15
Eco81I CCTNAGG 1 cut(s) 132
EcoRI GAATTC 1 cut(s) 47
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 33
EgeI GGCGCC 3 cut(s) 40, 192, 434
EheI GGCGCC 3 cut(s) 40, 192, 434
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 5 cut(s) 55, 88, 273, 740, 889
FaiI YATR 5 cut(s) 53, 86, 271, 738, 887
FalI AAGNNNNNCTT 4 cut(s) 281, 313, 492, 524
FatI CATG 5 cut(s) 51, 84, 269, 736, 885
FauI CCCGC 3 cut(s) 28, 40, 227
FbaI TGATCA 1 cut(s) 888
Fnu4HI GCNGC 9 cut(s) 128, 141, 171, 189, 436, 621, 717, 741, 744
FokI GGATG 3 cut(s) 194, 519, 696
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 9 cut(s) 128, 141, 171, 189, 436, 621, 717, 741, 744
FspBI CTAG 2 cut(s) 444, 659
GlaI GCGC 6 cut(s) 40, 68, 192, 248, 410, 434
GluI GCNGC 9 cut(s) 128, 141, 171, 189, 436, 621, 717, 741, 744
HaeII RGCGCY 3 cut(s) 42, 194, 436
HaeIII GGCC 2 cut(s) 140, 731
HapII CCGG 5 cut(s) 42, 174, 195, 412, 430
HgaI GACGC 3 cut(s) 85, 194, 883
HhaI GCGC 6 cut(s) 41, 69, 193, 249, 411, 435
Hin1I GRCGYC 4 cut(s) 39, 191, 216, 433
Hin1II CATG 5 cut(s) 55, 88, 273, 740, 889
Hin6I GCGC 6 cut(s) 39, 67, 191, 247, 409, 433
HinP1I GCGC 6 cut(s) 39, 67, 191, 247, 409, 433
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 7 cut(s) 80, 238, 361, 373, 643, 818, 867
HpaII CCGG 5 cut(s) 42, 174, 195, 412, 430
HphI GGTGA 2 cut(s) 451, 889
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 3 cut(s) 208, 559, 754
Hpy188III TCNNGA 8 cut(s) 134, 242, 345, 355, 512, 680, 853, 864
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 4 cut(s) 10, 29, 218, 221
HpyAV CCTTC 1 cut(s) 731
HpyCH4III ACNGT 4 cut(s) 25, 472, 610, 831
HpyCH4IV ACGT 2 cut(s) 14, 216
HpyCH4V TGCA 2 cut(s) 310, 746
HpyF3I CTNAG 4 cut(s) 132, 375, 525, 699
HpySE526I ACGT 2 cut(s) 14, 216
Hsp92I GRCGYC 4 cut(s) 39, 191, 216, 433
Hsp92II CATG 5 cut(s) 55, 88, 273, 740, 889
HspAI GCGC 6 cut(s) 39, 67, 191, 247, 409, 433
KasI GGCGCC 3 cut(s) 38, 190, 432
KroI GCCGGC 1 cut(s) 429
KroNI GCCGGC 1 cut(s) 431
Ksp22I TGATCA 1 cut(s) 888
KspI CCGCGG 1 cut(s) 36
Kzo9I GATC 4 cut(s) 339, 440, 754, 888
LguI GCTCTTC 3 cut(s) 86, 276, 489
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 4 cut(s) 139, 607, 703, 730
LweI GCATC 2 cut(s) 530, 541
MaeI CTAG 2 cut(s) 444, 659
MaeII ACGT 2 cut(s) 14, 216
MaeIII GTNAC 2 cut(s) 197, 895
MalI GATC 4 cut(s) 341, 442, 756, 890
MboI GATC 4 cut(s) 339, 440, 754, 888
MboII GAAGA 6 cut(s) 103, 293, 370, 442, 500, 506
MflI RGATCY 2 cut(s) 339, 440
MhlI GDGCHC 4 cut(s) 14, 20, 33, 288
MluCI AATT 2 cut(s) 47, 226
Mly113I GGCGCC 3 cut(s) 39, 191, 433
MlyI GAGTC 3 cut(s) 355, 367, 827
MmeI TCCRAC 3 cut(s) 171, 503, 582
MreI CGCCGGCG 1 cut(s) 429
MroNI GCCGGC 1 cut(s) 429
MspA1I CMGCKG 4 cut(s) 35, 620, 716, 743
MspI CCGG 5 cut(s) 42, 174, 195, 412, 430
MspR9I CCNGG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 841
MvnI CGCG 5 cut(s) 35, 69, 188, 243, 249
NaeI GCCGGC 1 cut(s) 431
NarI GGCGCC 3 cut(s) 39, 191, 433
NciI CCSGG 1 cut(s) 195
NcoI CCATGG 1 cut(s) 269
NdeII GATC 4 cut(s) 339, 440, 754, 888
NgoMIV GCCGGC 1 cut(s) 429
NlaIII CATG 5 cut(s) 55, 88, 273, 740, 889
NlaIV GGNNCC 9 cut(s) 40, 166, 192, 267, 434, 591, 732, 780, 828
NmeAIII GCCGAG 1 cut(s) 707
NmuCI GTSAC 1 cut(s) 895
NruI TCGCGA 1 cut(s) 243
PciSI GCTCTTC 3 cut(s) 86, 276, 489
PcsI WCGNNNNNNNCGW 3 cut(s) 11, 66, 827
PctI GAATGC 1 cut(s) 841
PdiI GCCGGC 1 cut(s) 431
PfeI GAWTC 4 cut(s) 80, 238, 643, 867
PflFI GACNNNGTC 2 cut(s) 218, 859
PkrI GCNGC 9 cut(s) 129, 142, 172, 190, 437, 622, 718, 742, 745
PleI GAGTC 3 cut(s) 355, 367, 826
PluTI GGCGCC 3 cut(s) 42, 194, 436
PmaCI CACGTG 1 cut(s) 15
PmlI CACGTG 1 cut(s) 15
PpsI GAGTC 3 cut(s) 355, 367, 826
Ppu21I YACGTR 1 cut(s) 15
PspCI CACGTG 1 cut(s) 15
PspN4I GGNNCC 9 cut(s) 40, 166, 192, 267, 434, 591, 732, 780, 828
PspPI GGNCC 3 cut(s) 614, 730, 778
PsuI RGATCY 2 cut(s) 339, 440
PsyI GACNNNGTC 2 cut(s) 218, 859
PvuII CAGCTG 2 cut(s) 620, 716
RruI TCGCGA 1 cut(s) 243
RsaI GTAC 4 cut(s) 122, 401, 504, 691
RsaNI GTAC 4 cut(s) 121, 400, 503, 690
SacII CCGCGG 1 cut(s) 36
SapI GCTCTTC 3 cut(s) 86, 276, 489
SatI GCNGC 9 cut(s) 128, 141, 171, 189, 436, 621, 717, 741, 744
Sau3AI GATC 4 cut(s) 339, 440, 754, 888
Sau96I GGNCC 3 cut(s) 614, 730, 778
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 3 cut(s) 355, 367, 827
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 4 cut(s) 14, 20, 33, 288
SfaNI GCATC 2 cut(s) 530, 541
SfcI CTRYAG 1 cut(s) 609
SfoI GGCGCC 3 cut(s) 40, 192, 434
Sfr303I CCGCGG 1 cut(s) 36
SgrAI CRCCGGYG 1 cut(s) 429
SgrBI CCGCGG 1 cut(s) 36
SinI GGWCC 2 cut(s) 614, 778
SmlI CTYRAG 2 cut(s) 343, 835
SmoI CTYRAG 2 cut(s) 343, 835
Sse9I AATT 2 cut(s) 47, 226
SspDI GGCGCC 3 cut(s) 38, 190, 432
SspMI CTAG 2 cut(s) 444, 659
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 4 cut(s) 25, 472, 610, 831
TaiI ACGT 2 cut(s) 17, 219
TaqI TCGA 4 cut(s) 27, 78, 453, 821
TasI AATT 2 cut(s) 47, 226
TatI WGTACW 2 cut(s) 399, 502
TauI GCSGC 5 cut(s) 143, 173, 191, 438, 743
TfiI GAWTC 4 cut(s) 80, 238, 643, 867
TscAI CASTG 2 cut(s) 580, 591
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 4 cut(s) 127, 620, 716, 743
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 1 cut(s) 40
TspGWI ACGGA 3 cut(s) 168, 290, 665
TspRI CASTG 2 cut(s) 580, 591
Tth111I GACNNNGTC 2 cut(s) 218, 859
VpaK11BI GGWCC 2 cut(s) 614, 778
XapI RAATTY 1 cut(s) 47
XspI CTAG 2 cut(s) 444, 659
ZraI GACGTC 1 cut(s) 217
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.