MD14G1189300.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003904
Physical Location & Seq
Forward (+)
28086717 .. 28088692
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1189300.v1.1.491

Sequence Viewer

Length: 909 bp
ATGTCGTCCGGCACGTGCTCCACCGTCGAGCCCGCGGGCGCGGGAGAGATCATGCTGTTCGGCGTGCGCTTGGTGGTCGATTCCATGAGGAAGAGCGTCAGTTTGAACAATCTCTCGCAGTACGAGCAGCCTCAGGAGGCCGCCTCCAACAACGGAAATAACGGTACCGCCGCCGGAAAGGATGACGCGGCGCCCGGTTACGCCTCTGAGAACGACGTCGTTCACAATTCTGGCGGGAATCGCGAGCGCGAACGCAAGCGAGGGGTTCCATGGACGGAGGAAGAGCACAAGCTTTTCTTGCTTGGATTGCAGAAAGTAGGGAAAGGAGATTGGAGAGGGATCTCAAGAAACTTCGTGAAGACTCGCACCCCGACTCAGGTTGCCAGCCACGCACAGAAATACTATCTGCGCCGGAGCAACCTCAATCGCCGGCGCCGCAGGTCTAGCCTCTTCGACATCACCACTGATACGGTCGCTCCAACTCCAATGGATGAAGAGCAAGTACAGCATCAAGATAACATATCTCAGTCCCAGTTGCATCCGTTGCCACCCCCGCCACCATCCGAGCCTCGCAATGCTGGTGGATTTTCGATGGTGCCAAATTTTGCAAGGACTGTAGGTCCAGCTGTCTTGCCTGTTCACATTGAGAACCCAATGGGAAATCTAGCTCTACGACAAGCAAATCCCGAGAATAGTACTTCGGCCAAGCTCGTCCATCCAGTTGCGCTTCATTCGGCCCCTCATGCGACCGCAATATCTGACCTAAACTTGAACTCAACGACGGACACATCGACCCTAACTCTCAACCTCTCCTTGTCAATGGACTCGAGGGAACCGTCGTCAAGGCATTCGGCTTTCCAGACAATGCCAGGATTCAGCAACGGGGATAGCATGATCAGTGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

303

Amino Acids

32.77

Weight (kDa)

7.97

Isoelectric Point (pI)

58.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 89 - 134 2.7e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
Acc36I ACCTGC 1 cut(s) 429
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 4 cut(s) 164, 190, 432, 595
AccII CGCG 5 cut(s) 35, 41, 188, 243, 249
AclWI GGATC 1 cut(s) 347
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 1 cut(s) 601
AcvI CACGTG 1 cut(s) 15
AcyI GRCGYC 3 cut(s) 191, 216, 433
AfaI GTAC 4 cut(s) 122, 166, 504, 697
AfiI CCNNNNNNNGG 1 cut(s) 376
AgsI TTSAA 2 cut(s) 106, 772
AhdI GACNNNNNGTC 1 cut(s) 618
AjnI CCWGG 1 cut(s) 868
AluBI AGCT 4 cut(s) 292, 626, 668, 709
AluI AGCT 4 cut(s) 292, 626, 668, 709
Alw21I GWGCWC 2 cut(s) 20, 288
AlwI GGATC 1 cut(s) 347
Ama87I CYCGRG 2 cut(s) 686, 826
AoxI GGCC 3 cut(s) 138, 702, 735
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 1 cut(s) 601
Asp718I GGTACC 1 cut(s) 164
AspLEI GCGC 7 cut(s) 41, 69, 193, 249, 411, 435, 727
AspS9I GGNCC 2 cut(s) 620, 736
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 1 cut(s) 451
AvaI CYCGRG 2 cut(s) 686, 826
AvaII GGWCC 1 cut(s) 620
AxyI CCTNAGG 1 cut(s) 132
BanI GGYRCC 4 cut(s) 164, 190, 432, 595
BanII GRGCYC 1 cut(s) 33
BarI GAAGNNNNNNTAC 2 cut(s) 486, 518
BbrPI CACGTG 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 2 cut(s) 20, 288
BbvI GCAGC 1 cut(s) 139
BccI CCATC 3 cut(s) 568, 586, 723
BciT130I CCWGG 1 cut(s) 870
BclI TGATCA 1 cut(s) 894
BcnI CCSGG 1 cut(s) 195
BfaI CTAG 2 cut(s) 444, 665
BfmI CTRYAG 1 cut(s) 615
BfoI RGCGCY 2 cut(s) 194, 436
BfuAI ACCTGC 1 cut(s) 429
BisI GCNGC 5 cut(s) 128, 141, 171, 189, 436
BlsI GCNGC 5 cut(s) 129, 142, 172, 190, 437
BmcAI AGTACT 1 cut(s) 697
Bme1390I CCNGG 2 cut(s) 195, 870
Bme18I GGWCC 1 cut(s) 620
BmeRI GACNNNNNGTC 1 cut(s) 618
BmeT110I CYCGRG 2 cut(s) 686, 826
BmgT120I GGNCC 2 cut(s) 620, 736
BmiI GGNNCC 7 cut(s) 166, 192, 267, 434, 597, 738, 834
BmrFI CCNGG 2 cut(s) 195, 870
BmrI ACTGGG 1 cut(s) 526
BmsI GCATC 2 cut(s) 517, 547
BmuI ACTGGG 1 cut(s) 526
BpiI GAAGAC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpuEI CTTGAG 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 15
BsaHI GRCGYC 3 cut(s) 191, 216, 433
BsaJI CCNNGG 2 cut(s) 33, 269
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse118I RCCGGY 1 cut(s) 429
Bse1I ACTGG 2 cut(s) 532, 719
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 580
BseBI CCWGG 1 cut(s) 870
BseDI CCNNGG 2 cut(s) 33, 269
BseGI GGATG 5 cut(s) 187, 496, 538, 560, 715
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 4 cut(s) 146, 198, 389, 539
BseNI ACTGG 2 cut(s) 532, 719
BseXI GCAGC 1 cut(s) 139
Bsh1236I CGCG 5 cut(s) 35, 41, 188, 243, 249
Bsh1285I CGRYCG 2 cut(s) 474, 750
BshFI GGCC 3 cut(s) 140, 704, 737
BshNI GGYRCC 4 cut(s) 164, 190, 432, 595
BsiEI CGRYCG 2 cut(s) 474, 750
BsiHKAI GWGCWC 2 cut(s) 20, 288
BsiHKCI CYCGRG 2 cut(s) 686, 826
BsiSI CCGG 5 cut(s) 9, 174, 195, 412, 430
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 376
BsmFI GGGAC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 3 cut(s) 140, 704, 737
BsoBI CYCGRG 2 cut(s) 686, 826
Bsp1286I GDGCHC 3 cut(s) 20, 33, 288
Bsp143I GATC 3 cut(s) 48, 339, 894
Bsp19I CCATGG 1 cut(s) 269
Bsp68I TCGCGA 1 cut(s) 243
BspANI GGCC 3 cut(s) 140, 704, 737
BspCNI CTCAG 4 cut(s) 145, 199, 388, 538
BspFNI CGCG 5 cut(s) 35, 41, 188, 243, 249
BspLI GGNNCC 7 cut(s) 166, 192, 267, 434, 597, 738, 834
BspMI ACCTGC 1 cut(s) 429
BspPI GGATC 1 cut(s) 347
BspQI GCTCTTC 3 cut(s) 86, 276, 489
BspT107I GGYRCC 4 cut(s) 164, 190, 432, 595
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 429
BsrI ACTGG 2 cut(s) 532, 719
BssAI RCCGGY 1 cut(s) 429
BssECI CCNNGG 2 cut(s) 33, 269
BssMI GATC 3 cut(s) 48, 339, 894
BssNI GRCGYC 3 cut(s) 191, 216, 433
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 870
Bst4CI ACNGT 5 cut(s) 25, 164, 472, 616, 837
Bst6I CTCTTC 4 cut(s) 86, 276, 455, 489
BstACI GRCGYC 3 cut(s) 191, 216, 433
BstAPI GCANNNNNTGC 1 cut(s) 544
BstBAI YACGTR 1 cut(s) 15
BstC8I GCNNGC 7 cut(s) 33, 37, 65, 245, 257, 385, 431
BstDEI CTNAG 4 cut(s) 132, 207, 375, 525
BstDSI CCRYGG 2 cut(s) 33, 269
BstF5I GGATG 5 cut(s) 187, 496, 538, 560, 715
BstFNI CGCG 5 cut(s) 35, 41, 188, 243, 249
BstH2I RGCGCY 2 cut(s) 194, 436
BstHHI GCGC 7 cut(s) 41, 69, 193, 249, 411, 435, 727
BstKTI GATC 3 cut(s) 51, 342, 897
BstMBI GATC 3 cut(s) 48, 339, 894
BstMCI CGRYCG 2 cut(s) 474, 750
BstNI CCWGG 1 cut(s) 870
BstSCI CCNGG 2 cut(s) 193, 868
BstSFI CTRYAG 1 cut(s) 615
BstUI CGCG 5 cut(s) 35, 41, 188, 243, 249
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 339
BstYI RGATCY 1 cut(s) 339
Bsu36I CCTNAGG 1 cut(s) 132
BsuRI GGCC 3 cut(s) 140, 704, 737
BtgI CCRYGG 2 cut(s) 33, 269
BtsCI GGATG 5 cut(s) 187, 496, 538, 560, 715
BtsIMutI CAGTG 2 cut(s) 462, 904
BtuMI TCGCGA 1 cut(s) 243
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 7 cut(s) 33, 37, 65, 245, 257, 385, 431
CfoI GCGC 7 cut(s) 41, 69, 193, 249, 411, 435, 727
Cfr10I RCCGGY 1 cut(s) 429
Cfr13I GGNCC 2 cut(s) 620, 736
Cfr42I CCGCGG 1 cut(s) 36
CseI GACGC 2 cut(s) 85, 194
Csp6I GTAC 4 cut(s) 121, 165, 503, 696
CspCI CAANNNNNGTGG 2 cut(s) 562, 597
CviAII CATG 5 cut(s) 52, 85, 270, 743, 892
CviQI GTAC 4 cut(s) 121, 165, 503, 696
DdeI CTNAG 4 cut(s) 132, 207, 375, 525
DinI GGCGCC 2 cut(s) 192, 434
DpnI GATC 3 cut(s) 50, 341, 896
DpnII GATC 3 cut(s) 48, 339, 894
DriI GACNNNNNGTC 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 4 cut(s) 86, 276, 455, 489
Eam1105I GACNNNNNGTC 1 cut(s) 618
EarI CTCTTC 4 cut(s) 86, 276, 455, 489
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 620
Eco72I CACGTG 1 cut(s) 15
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 2 cut(s) 686, 826
EcoRII CCWGG 1 cut(s) 868
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 33
EgeI GGCGCC 2 cut(s) 192, 434
EheI GGCGCC 2 cut(s) 192, 434
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 5 cut(s) 55, 88, 273, 746, 895
FaiI YATR 6 cut(s) 53, 86, 271, 521, 744, 893
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 1 cut(s) 514
FatI CATG 5 cut(s) 51, 84, 269, 742, 891
FauI CCCGC 5 cut(s) 28, 34, 40, 227, 561
FbaI TGATCA 1 cut(s) 894
Fnu4HI GCNGC 5 cut(s) 128, 141, 171, 189, 436
FokI GGATG 5 cut(s) 194, 503, 525, 547, 702
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 5 cut(s) 128, 141, 171, 189, 436
FspBI CTAG 2 cut(s) 444, 665
GlaI GCGC 7 cut(s) 40, 68, 192, 248, 410, 434, 726
GluI GCNGC 5 cut(s) 128, 141, 171, 189, 436
HaeII RGCGCY 2 cut(s) 194, 436
HaeIII GGCC 3 cut(s) 140, 704, 737
HapII CCGG 5 cut(s) 9, 174, 195, 412, 430
HgaI GACGC 2 cut(s) 85, 194
HhaI GCGC 7 cut(s) 41, 69, 193, 249, 411, 435, 727
Hin1I GRCGYC 3 cut(s) 191, 216, 433
Hin1II CATG 5 cut(s) 55, 88, 273, 746, 895
Hin6I GCGC 7 cut(s) 39, 67, 191, 247, 409, 433, 725
HinP1I GCGC 7 cut(s) 39, 67, 191, 247, 409, 433, 725
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 6 cut(s) 80, 238, 361, 373, 824, 873
HpaII CCGG 5 cut(s) 9, 174, 195, 412, 430
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 2 cut(s) 223, 640
Hpy188I TCNGA 3 cut(s) 208, 565, 760
Hpy188III TCNNGA 7 cut(s) 134, 242, 345, 355, 512, 686, 859
Hpy8I GTNNAC 2 cut(s) 223, 640
Hpy99I CGWCG 5 cut(s) 29, 218, 221, 784, 841
HpyCH4III ACNGT 5 cut(s) 25, 164, 472, 616, 837
HpyCH4IV ACGT 2 cut(s) 14, 216
HpyCH4V TGCA 3 cut(s) 310, 538, 608
HpyF3I CTNAG 4 cut(s) 132, 207, 375, 525
HpySE526I ACGT 2 cut(s) 14, 216
Hsp92I GRCGYC 3 cut(s) 191, 216, 433
Hsp92II CATG 5 cut(s) 55, 88, 273, 746, 895
HspAI GCGC 7 cut(s) 39, 67, 191, 247, 409, 433, 725
KasI GGCGCC 2 cut(s) 190, 432
KpnI GGTACC 1 cut(s) 168
KroI GCCGGC 1 cut(s) 429
KroNI GCCGGC 1 cut(s) 431
Ksp22I TGATCA 1 cut(s) 894
KspI CCGCGG 1 cut(s) 36
Kzo9I GATC 3 cut(s) 48, 339, 894
LguI GCTCTTC 3 cut(s) 86, 276, 489
LmnI GCTCC 3 cut(s) 23, 414, 481
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 2 cut(s) 517, 547
MaeI CTAG 2 cut(s) 444, 665
MaeII ACGT 2 cut(s) 14, 216
MaeIII GTNAC 1 cut(s) 197
MalI GATC 3 cut(s) 50, 341, 896
MboI GATC 3 cut(s) 48, 339, 894
MboII GAAGA 5 cut(s) 103, 293, 370, 442, 506
MflI RGATCY 1 cut(s) 339
MhlI GDGCHC 3 cut(s) 20, 33, 288
MluCI AATT 2 cut(s) 226, 601
Mly113I GGCGCC 2 cut(s) 191, 433
MlyI GAGTC 3 cut(s) 355, 367, 818
MmeI TCCRAC 2 cut(s) 171, 503
MreI CGCCGGCG 1 cut(s) 429
MroNI GCCGGC 1 cut(s) 429
MspA1I CMGCKG 2 cut(s) 35, 626
MspI CCGG 5 cut(s) 9, 174, 195, 412, 430
MspR9I CCNGG 2 cut(s) 195, 870
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 1 cut(s) 870
MvnI CGCG 5 cut(s) 35, 41, 188, 243, 249
NaeI GCCGGC 1 cut(s) 431
NarI GGCGCC 2 cut(s) 191, 433
NciI CCSGG 1 cut(s) 195
NcoI CCATGG 1 cut(s) 269
NdeII GATC 3 cut(s) 48, 339, 894
NgoMIV GCCGGC 1 cut(s) 429
NlaIII CATG 5 cut(s) 55, 88, 273, 746, 895
NlaIV GGNNCC 7 cut(s) 166, 192, 267, 434, 597, 738, 834
NruI TCGCGA 1 cut(s) 243
PaeR7I CTCGAG 1 cut(s) 826
PciSI GCTCTTC 3 cut(s) 86, 276, 489
PcsI WCGNNNNNNNCGW 3 cut(s) 11, 788, 833
PctI GAATGC 1 cut(s) 847
PdiI GCCGGC 1 cut(s) 431
PfeI GAWTC 3 cut(s) 80, 238, 873
PkrI GCNGC 5 cut(s) 129, 142, 172, 190, 437
PleI GAGTC 3 cut(s) 355, 367, 818
PluTI GGCGCC 2 cut(s) 194, 436
PmaCI CACGTG 1 cut(s) 15
PmlI CACGTG 1 cut(s) 15
PpsI GAGTC 3 cut(s) 355, 367, 818
Ppu21I YACGTR 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 868
PspCI CACGTG 1 cut(s) 15
PspGI CCWGG 1 cut(s) 868
PspN4I GGNNCC 7 cut(s) 166, 192, 267, 434, 597, 738, 834
PspPI GGNCC 2 cut(s) 620, 736
PspXI VCTCGAGB 1 cut(s) 826
PsuI RGATCY 1 cut(s) 339
PvuII CAGCTG 1 cut(s) 626
RruI TCGCGA 1 cut(s) 243
RsaI GTAC 4 cut(s) 122, 166, 504, 697
RsaNI GTAC 4 cut(s) 121, 165, 503, 696
SacII CCGCGG 1 cut(s) 36
SapI GCTCTTC 3 cut(s) 86, 276, 489
SatI GCNGC 5 cut(s) 128, 141, 171, 189, 436
Sau3AI GATC 3 cut(s) 48, 339, 894
Sau96I GGNCC 2 cut(s) 620, 736
ScaI AGTACT 1 cut(s) 697
SchI GAGTC 3 cut(s) 355, 367, 818
ScrFI CCNGG 2 cut(s) 195, 870
SduI GDGCHC 3 cut(s) 20, 33, 288
SfaNI GCATC 2 cut(s) 517, 547
SfcI CTRYAG 1 cut(s) 615
SfoI GGCGCC 2 cut(s) 192, 434
Sfr274I CTCGAG 1 cut(s) 826
Sfr303I CCGCGG 1 cut(s) 36
SgrAI CRCCGGYG 1 cut(s) 429
SgrBI CCGCGG 1 cut(s) 36
SinI GGWCC 1 cut(s) 620
SlaI CTCGAG 1 cut(s) 826
SmlI CTYRAG 2 cut(s) 343, 826
SmoI CTYRAG 2 cut(s) 343, 826
Sse9I AATT 2 cut(s) 226, 601
SspDI GGCGCC 2 cut(s) 190, 432
SspMI CTAG 2 cut(s) 444, 665
StyD4I CCNGG 2 cut(s) 193, 868
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 5 cut(s) 25, 164, 472, 616, 837
TaiI ACGT 2 cut(s) 17, 219
TaqI TCGA 6 cut(s) 27, 78, 453, 590, 791, 827
TasI AATT 2 cut(s) 226, 601
TatI WGTACW 2 cut(s) 502, 695
TauI GCSGC 4 cut(s) 143, 173, 191, 438
TfiI GAWTC 3 cut(s) 80, 238, 873
TscAI CASTG 2 cut(s) 469, 904
TseI GCWGC 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 507, 719
TspGWI ACGGA 4 cut(s) 168, 290, 531, 797
TspRI CASTG 2 cut(s) 469, 904
VpaK11BI GGWCC 1 cut(s) 620
XapI RAATTY 1 cut(s) 601
XhoI CTCGAG 1 cut(s) 826
XspI CTAG 2 cut(s) 444, 665
ZraI GACGTC 1 cut(s) 217
ZrmI AGTACT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.