MD01G1067400.v1.1

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003426
Physical Location & Seq
Reverse (-)
17066078 .. 17067528
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1067400.v1.1.491

Sequence Viewer

Length: 909 bp
ATGCGGATCAGGAAGAACGCCAAGGTCTCTCCCCTTCTGTTCACCTCGCATGCTCCAGCTGCTGGGGACCTCCATCCGCACGTGTGCCAGCTCAACCGGAACCCCTGGGATGTTATTCCTTTGGGACCGGAGTCCGACCAGTGGGAGGTCGAAGAGAGCTTTTACGGTGGGAGCTTCGGTGATTCAATCGGAGCTGTCGAGAGCGTGGCGTCAATGAGGGATGCCACGGAGGAAAGACGCTTGGAAATGATAGAAGAGCCCGTGGCGGAGGATGTCGAATTCGAAAAGGATTCGAATAAGGCGGAGGGCGAGATGGAGGTGGAAGAAGGGAGAATGGGCAGCATGTGCTGCCAACGGAACGACGGCCGTGGGTGGCAATGCAAGAAAAAAGCCAAGCAAGGGCAGTCCTTCTGCAACCACCACCTCTCCCTACTTCGATCGAACTACATTGTCAAGGCCAACGGGAATGGAAACAGTAGGGATAATGGAATTAATCACAACATTTCTTCGAGTAAGGCTCCTGCCGCGGTGAGTGGGGTGGTTCAGCTGGAAAATGGGGGCCGCCGCCGCCGGAAGAAGGCGAAGAAGGAATCCGCCTCTAACCCCTACGAGTTCTATTACTATTCGGGGTTTGGCCCGTGTCGGAACCGGAAGAGAGGCTGCGATAAAGAAAGGGAAGAGACCAAGGTCGTGGGTGAAATTGCTACTAATACCAGCTCTGCTACTACTTCTCCTCCTCCTCCTCCTCCTCATGAGAGCGGTCCTCGCTCACCCTTGTCTCCAATTGCGAATGGGGAATTCAATTATGACGACGAGGATGATGATGATGAGCTCGACGGCAGCGGCGAGAGCGGGAGGAGGCGGATGAGGAAGCCGGTGAAGGAAAGATCGCTCAAGTCCCTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

303

Amino Acids

33.55

Weight (kDa)

6.23

Isoelectric Point (pI)

59.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 116 - 148 3.4e-09 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 62
AccBSI CCGCTC 2 cut(s) 759, 852
AccII CGCG 1 cut(s) 527
AclWI GGATC 1 cut(s) 14
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 2 cut(s) 278, 797
AcvI CACGTG 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 209
AfiI CCNNNNNNNGG 5 cut(s) 62, 141, 145, 399, 577
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 2 cut(s) 186, 802
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 8 cut(s) 59, 91, 159, 174, 194, 547, 717, 832
AluI AGCT 8 cut(s) 59, 91, 159, 174, 194, 547, 717, 832
Alw21I GWGCWC 1 cut(s) 834
Alw26I GTCTC 3 cut(s) 31, 674, 783
AlwI GGATC 1 cut(s) 14
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 4 cut(s) 364, 456, 559, 634
ApeKI GCWGC 5 cut(s) 59, 339, 348, 660, 840
ApoI RAATTY 2 cut(s) 278, 797
AseI ATTAAT 1 cut(s) 492
AspS9I GGNCC 5 cut(s) 67, 125, 559, 635, 761
AsuHPI GGTGA 6 cut(s) 34, 191, 541, 707, 762, 889
AsuII TTCGAA 2 cut(s) 282, 293
AvaII GGWCC 3 cut(s) 67, 125, 761
BanII GRGCYC 2 cut(s) 261, 834
BbrPI CACGTG 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 834
BbvI GCAGC 5 cut(s) 46, 335, 351, 647, 852
BccI CCATC 2 cut(s) 81, 307
BceAI ACGGC 3 cut(s) 351, 379, 853
BciT130I CCWGG 1 cut(s) 106
BcoDI GTCTC 3 cut(s) 31, 674, 783
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 3 cut(s) 67, 125, 761
BmgT120I GGNCC 5 cut(s) 67, 125, 559, 635, 761
BmiI GGNNCC 6 cut(s) 68, 101, 126, 519, 560, 647
BmrFI CCNGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 211
BoxI GACNNNNGTC 2 cut(s) 130, 686
BplI GAGNNNNNCTC 4 cut(s) 502, 534, 748, 780
BpmI CTGGAG 1 cut(s) 39
Bpu14I TTCGAA 2 cut(s) 282, 293
BpuEI CTTGAG 1 cut(s) 878
BsaAI YACGTR 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 209
BsaI GGTCTC 2 cut(s) 31, 674
BsaJI CCNNGG 8 cut(s) 21, 104, 105, 225, 261, 367, 525, 684
BsaWI WCCGGW 3 cut(s) 96, 127, 648
BsaXI ACNNNNNCTCC 8 cut(s) 163, 193, 410, 440, 715, 718, 745, 748
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 141, 145, 399, 577
Bse118I RCCGGY 1 cut(s) 874
Bse1I ACTGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 383
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 8 cut(s) 21, 104, 105, 225, 261, 367, 525, 684
BseGI GGATG 6 cut(s) 73, 115, 226, 277, 823, 870
BseLI CCNNNNNNNGG 5 cut(s) 62, 141, 145, 399, 577
BseMI GCAATG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 7 cut(s) 723, 726, 729, 732, 735, 738, 871
BseX3I CGGCCG 1 cut(s) 364
BseXI GCAGC 5 cut(s) 46, 335, 351, 647, 852
BseYI CCCAGC 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 527
Bsh1285I CGRYCG 2 cut(s) 367, 440
BshFI GGCC 4 cut(s) 366, 458, 561, 636
BsiEI CGRYCG 2 cut(s) 367, 440
BsiHKAI GWGCWC 1 cut(s) 834
BsiSI CCGG 5 cut(s) 97, 128, 571, 649, 875
BslFI GGGAC 3 cut(s) 80, 138, 883
BslI CCNNNNNNNGG 5 cut(s) 62, 141, 145, 399, 577
BsmAI GTCTC 3 cut(s) 31, 674, 783
BsmFI GGGAC 3 cut(s) 80, 138, 883
BsnI GGCC 4 cut(s) 366, 458, 561, 636
Bso31I GGTCTC 2 cut(s) 31, 674
Bsp119I TTCGAA 2 cut(s) 282, 293
Bsp1286I GDGCHC 2 cut(s) 261, 834
Bsp143I GATC 3 cut(s) 6, 437, 887
BspANI GGCC 4 cut(s) 366, 458, 561, 636
BspFNI CGCG 1 cut(s) 527
BspHI TCATGA 1 cut(s) 751
BspLI GGNNCC 6 cut(s) 68, 101, 126, 519, 560, 647
BspPI GGATC 1 cut(s) 14
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 2 cut(s) 282, 293
BspTNI GGTCTC 2 cut(s) 31, 674
BsrBI CCGCTC 2 cut(s) 759, 852
BsrDI GCAATG 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 874
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 874
BssECI CCNNGG 8 cut(s) 21, 104, 105, 225, 261, 367, 525, 684
BssMI GATC 3 cut(s) 6, 437, 887
BssNI GRCGYC 1 cut(s) 209
BssT1I CCWWGG 2 cut(s) 21, 684
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 167, 476
Bst6I CTCTTC 4 cut(s) 147, 249, 647, 672
BstACI GRCGYC 1 cut(s) 209
BstAPI GCANNNNNTGC 2 cut(s) 345, 348
BstBAI YACGTR 1 cut(s) 82
BstBI TTCGAA 2 cut(s) 282, 293
BstC8I GCNNGC 2 cut(s) 51, 89
BstDSI CCRYGG 4 cut(s) 225, 261, 367, 525
BstF5I GGATG 6 cut(s) 73, 115, 226, 277, 823, 870
BstFNI CGCG 1 cut(s) 527
BstKTI GATC 3 cut(s) 9, 440, 890
BstMAI GTCTC 3 cut(s) 31, 674, 783
BstMBI GATC 3 cut(s) 6, 437, 887
BstMCI CGRYCG 2 cut(s) 367, 440
BstMWI GCNNNNNNNGC 7 cut(s) 59, 345, 348, 524, 567, 765, 849
BstNI CCWGG 1 cut(s) 106
BstNSI RCATGY 2 cut(s) 53, 346
BstPAI GACNNNNGTC 2 cut(s) 130, 686
BstSCI CCNGG 1 cut(s) 104
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 5 cut(s) 46, 335, 351, 647, 852
BstXI CCANNNNNNTGG 1 cut(s) 691
BstZI CGGCCG 1 cut(s) 364
BsuRI GGCC 4 cut(s) 366, 458, 561, 636
BtgI CCRYGG 4 cut(s) 225, 261, 367, 525
BtsCI GGATG 6 cut(s) 73, 115, 226, 277, 823, 870
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 2 cut(s) 51, 89
CaiI CAGNNNCTG 1 cut(s) 62
CciI TCATGA 1 cut(s) 751
Cfr10I RCCGGY 1 cut(s) 874
Cfr13I GGNCC 5 cut(s) 67, 125, 559, 635, 761
Cfr42I CCGCGG 1 cut(s) 528
CseI GACGC 2 cut(s) 198, 246
CviAII CATG 3 cut(s) 50, 343, 752
DpnI GATC 3 cut(s) 8, 439, 889
DpnII GATC 3 cut(s) 6, 437, 887
EaeI YGGCCR 1 cut(s) 364
EagI CGGCCG 1 cut(s) 364
Eam1104I CTCTTC 4 cut(s) 147, 249, 647, 672
EarI CTCTTC 4 cut(s) 147, 249, 647, 672
EciI GGCGGA 4 cut(s) 281, 317, 583, 877
Ecl136II GAGCTC 1 cut(s) 832
EclXI CGGCCG 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 21, 684
Eco24I GRGCYC 2 cut(s) 261, 834
Eco31I GGTCTC 2 cut(s) 31, 674
Eco47I GGWCC 3 cut(s) 67, 125, 761
Eco52I CGGCCG 1 cut(s) 364
Eco53kI GAGCTC 1 cut(s) 832
Eco72I CACGTG 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 832
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 2 cut(s) 278, 797
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 2 cut(s) 21, 684
EcoT38I GRGCYC 2 cut(s) 261, 834
ErhI CCWWGG 2 cut(s) 21, 684
FaeI CATG 3 cut(s) 53, 346, 755
FaiI YATR 4 cut(s) 51, 344, 753, 807
FaqI GGGAC 3 cut(s) 80, 138, 883
FatI CATG 3 cut(s) 49, 342, 751
FauI CCCGC 1 cut(s) 845
FokI GGATG 6 cut(s) 60, 122, 233, 284, 830, 877
FriOI GRGCYC 2 cut(s) 261, 834
GsaI CCCAGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 4 cut(s) 366, 458, 561, 636
HapII CCGG 5 cut(s) 97, 128, 571, 649, 875
HgaI GACGC 2 cut(s) 198, 246
Hin1I GRCGYC 1 cut(s) 209
Hin1II CATG 3 cut(s) 53, 346, 755
HinfI GANTC 4 cut(s) 131, 182, 290, 590
HpaII CCGG 5 cut(s) 97, 128, 571, 649, 875
HphI GGTGA 6 cut(s) 34, 191, 541, 707, 762, 889
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 3 cut(s) 136, 191, 645
Hpy188III TCNNGA 3 cut(s) 10, 199, 752
Hpy8I GTNNAC 1 cut(s) 42
Hpy99I CGWCG 3 cut(s) 365, 815, 839
HpyAV CCTTC 6 cut(s) 44, 320, 418, 571, 580, 874
HpyCH4III ACNGT 2 cut(s) 167, 476
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 381, 414
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 345, 348, 524, 567, 765, 849
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 209
Hsp92II CATG 3 cut(s) 53, 346, 755
KspI CCGCGG 1 cut(s) 528
Kzo9I GATC 3 cut(s) 6, 437, 887
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 4 cut(s) 58, 171, 191, 523
Lsp1109I GCAGC 5 cut(s) 46, 335, 351, 647, 852
LweI GCATC 1 cut(s) 211
MaeII ACGT 1 cut(s) 81
MalI GATC 3 cut(s) 8, 439, 889
MbiI CCGCTC 2 cut(s) 759, 852
MboI GATC 3 cut(s) 6, 437, 887
MboII GAAGA 9 cut(s) 25, 164, 266, 335, 498, 586, 595, 664, 689
MfeI CAATTG 1 cut(s) 783
MhlI GDGCHC 2 cut(s) 261, 834
MluCI AATT 6 cut(s) 278, 489, 699, 783, 797, 802
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 2 cut(s) 159, 623
MseI TTAA 1 cut(s) 492
MspA1I CMGCKG 4 cut(s) 59, 527, 547, 843
MspI CCGG 5 cut(s) 97, 128, 571, 649, 875
MspR9I CCNGG 1 cut(s) 106
MunI CAATTG 1 cut(s) 783
MvaI CCWGG 1 cut(s) 106
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 7 cut(s) 59, 345, 348, 524, 567, 765, 849
NdeII GATC 3 cut(s) 6, 437, 887
NlaIII CATG 3 cut(s) 53, 346, 755
NlaIV GGNNCC 6 cut(s) 68, 101, 126, 519, 560, 647
NspI RCATGY 2 cut(s) 53, 346
NspV TTCGAA 2 cut(s) 282, 293
PaeI GCATGC 1 cut(s) 53
PagI TCATGA 1 cut(s) 751
PasI CCCWGGG 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 843
PfeI GAWTC 3 cut(s) 182, 290, 590
PflMI CCANNNNNTGG 1 cut(s) 62
Ple19I CGATCG 1 cut(s) 440
PleI GAGTC 1 cut(s) 139
PmaCI CACGTG 1 cut(s) 82
PmlI CACGTG 1 cut(s) 82
PpsI GAGTC 1 cut(s) 139
Ppu21I YACGTR 1 cut(s) 82
PpuMI RGGWCCY 1 cut(s) 67
PshAI GACNNNNGTC 2 cut(s) 130, 686
PshBI ATTAAT 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 834
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 104
PspCI CACGTG 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 6 cut(s) 68, 101, 126, 519, 560, 647
PspPI GGNCC 5 cut(s) 67, 125, 559, 635, 761
PspPPI RGGWCCY 1 cut(s) 67
PstNI CAGNNNCTG 1 cut(s) 62
PvuI CGATCG 1 cut(s) 440
PvuII CAGCTG 2 cut(s) 59, 547
SacI GAGCTC 1 cut(s) 834
SacII CCGCGG 1 cut(s) 528
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 492
Sau3AI GATC 3 cut(s) 6, 437, 887
Sau96I GGNCC 5 cut(s) 67, 125, 559, 635, 761
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 2 cut(s) 261, 834
SfaNI GCATC 1 cut(s) 211
Sfr303I CCGCGG 1 cut(s) 528
SfuI TTCGAA 2 cut(s) 282, 293
SgrBI CCGCGG 1 cut(s) 528
SinI GGWCC 3 cut(s) 67, 125, 761
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
SphI GCATGC 1 cut(s) 53
Sse9I AATT 6 cut(s) 278, 489, 699, 783, 797, 802
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 2 cut(s) 21, 684
TaaI ACNGT 2 cut(s) 167, 476
TaiI ACGT 1 cut(s) 84
TaqI TCGA 9 cut(s) 150, 198, 276, 282, 293, 436, 440, 509, 834
TasI AATT 6 cut(s) 278, 489, 699, 783, 797, 802
TauI GCSGC 5 cut(s) 527, 564, 567, 570, 846
TfiI GAWTC 3 cut(s) 182, 290, 590
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 1 cut(s) 146
TseI GCWGC 5 cut(s) 59, 339, 348, 660, 840
TspGWI ACGGA 2 cut(s) 242, 370
TspRI CASTG 1 cut(s) 146
Van91I CCANNNNNTGG 1 cut(s) 62
VpaK11BI GGWCC 3 cut(s) 67, 125, 761
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 2 cut(s) 278, 797
XceI RCATGY 2 cut(s) 53, 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.