pycom07g13110

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003426
Physical Location & Seq
Forward (+)
14700631 .. 14702079
1449 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g13110.1

Sequence Viewer

Length: 870 bp
ATGCGGATCAGGAAGAACGCCAAGGTCTCTCCCCTTCTGTTCACCTCGCAAGCTCCAGAAGCCGAGGAGCTTCTACCGCATGTGTGCCAGATCAACCGGAACCCCTGGGATGTTATTCCTTTGGGACCGGAGTCTGACCAGTGGGAGGTCGAAGAGAGCTTGTACGGTGGGAGCTTCGGTGATTCAATCGGAGCCGTCGAGAGCGTCGCGTCGTTGAGGGATGGCGCGGACGAGAAACCCTTGGAAATGAATGAAGGGCCCGCGGCGGAGAGTATCAACATTGAGAACAATTGGAAGGTGGGCGACGAGAGGACGGGGAGCATGTGCTGCCAACGGAATGACGGCCGTGGGTGGCATTGTAAGAAAAAAGCCAAGCAAGGGGAGTCCTTCTGCAACCATCACCTGTCCCTACTTCGATCGGACTGCATTGTCAAGGCCAACGGCAATGGATACTGTAGGGATAATGGAAATAATCACAACATATCTTCGAAGAAGGCTGCCGCCGCGGTGAGCGGGGTGGTACAGCTGGAAAATGGAGGCGGCGGCCGCCGGAAGAAGGTGAAGAAGGAATCCGCCCCTAACCCCTACGAGTTCTATTACTATTCGGGATTTGGCCCTTGGCGGAGTCGGAAGAGAGGCGGCGACAAAGAAAAGGAAGAGACCAAGGCCGAGGGTGAAATTGCTACTACGACTAGCTTTCCTCCTCCTCCTCATGAGAGCGCTCATCGGTCACCCTCGTCTCCAATTGCAAATGGGGAATTCGATTATGACGACGAGGATGACGACGATGAGCTCAATGGCAGCGGCGTTAGCAGCAGGAAGCGGATGAGGAAGCCGGTGAAGGAGAGATCGCTCAAGTCCCTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

290

Amino Acids

31.93

Weight (kDa)

6.22

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 109 - 139 3.3e-08 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 428
AccBSI CCGCTC 1 cut(s) 513
AccII CGCG 4 cut(s) 209, 227, 263, 506
AclWI GGATC 1 cut(s) 14
AcoI YGGCCR 2 cut(s) 343, 544
AcsI RAATTY 1 cut(s) 758
AfaI GTAC 2 cut(s) 164, 522
AfeI AGCGCT 1 cut(s) 721
AfiI CCNNNNNNNGG 3 cut(s) 145, 378, 556
AgsI TTSAA 1 cut(s) 186
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 7 cut(s) 53, 70, 159, 174, 526, 696, 793
AluI AGCT 7 cut(s) 53, 70, 159, 174, 526, 696, 793
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 3 cut(s) 31, 653, 744
AlwI GGATC 1 cut(s) 14
Aor51HI AGCGCT 1 cut(s) 721
AoxI GGCC 6 cut(s) 257, 343, 435, 544, 613, 666
ApaI GGGCCC 1 cut(s) 261
ApeKI GCWGC 4 cut(s) 327, 497, 801, 813
ApoI RAATTY 1 cut(s) 758
AspLEI GCGC 2 cut(s) 227, 722
AspS9I GGNCC 4 cut(s) 125, 257, 258, 614
AsuHPI GGTGA 8 cut(s) 34, 191, 392, 520, 571, 686, 723, 850
AsuII TTCGAA 1 cut(s) 488
AvaII GGWCC 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 261
BanII GRGCYC 2 cut(s) 261, 795
Bbv12I GWGCWC 1 cut(s) 795
BbvI GCAGC 4 cut(s) 314, 484, 813, 825
BccI CCATC 2 cut(s) 215, 405
BceAI ACGGC 4 cut(s) 179, 330, 358, 457
BcgI CGANNNNNNTGC 2 cut(s) 405, 439
BciT130I CCWGG 1 cut(s) 106
BciVI GTATCC 1 cut(s) 443
BcoDI GTCTC 3 cut(s) 31, 653, 744
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 1 cut(s) 454
BfoI RGCGCY 1 cut(s) 723
BfuI GTATCC 1 cut(s) 443
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 4 cut(s) 125, 257, 258, 614
BmiI GGNNCC 4 cut(s) 101, 126, 193, 259
BmrFI CCNGG 1 cut(s) 106
BoxI GACNNNNGTC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 839
BsaI GGTCTC 2 cut(s) 31, 653
BsaWI WCCGGW 2 cut(s) 96, 127
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 378, 556
Bse118I RCCGGY 1 cut(s) 835
Bse1I ACTGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 451
BseBI CCWGG 1 cut(s) 106
BseGI GGATG 4 cut(s) 115, 226, 784, 831
BseLI CCNNNNNNNGG 3 cut(s) 145, 378, 556
BseMI GCAATG 1 cut(s) 451
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 4 cut(s) 80, 693, 696, 699
BseSI GKGCMC 1 cut(s) 261
BseX3I CGGCCG 2 cut(s) 343, 544
BseXI GCAGC 4 cut(s) 314, 484, 813, 825
Bsh1236I CGCG 4 cut(s) 209, 227, 263, 506
Bsh1285I CGRYCG 3 cut(s) 346, 419, 547
BshFI GGCC 6 cut(s) 259, 345, 437, 546, 615, 668
BsiEI CGRYCG 3 cut(s) 346, 419, 547
BsiHKAI GWGCWC 1 cut(s) 795
BsiSI CCGG 4 cut(s) 97, 128, 550, 836
BslFI GGGAC 3 cut(s) 138, 391, 844
BslI CCNNNNNNNGG 3 cut(s) 145, 378, 556
BsmAI GTCTC 3 cut(s) 31, 653, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 3 cut(s) 138, 391, 844
BsnI GGCC 6 cut(s) 259, 345, 437, 546, 615, 668
Bso31I GGTCTC 2 cut(s) 31, 653
Bsp119I TTCGAA 1 cut(s) 488
Bsp120I GGGCCC 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 261, 795
Bsp143I GATC 4 cut(s) 6, 90, 416, 848
BspANI GGCC 6 cut(s) 259, 345, 437, 546, 615, 668
BspFNI CGCG 4 cut(s) 209, 227, 263, 506
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 4 cut(s) 101, 126, 193, 259
BspPI GGATC 1 cut(s) 14
BspT104I TTCGAA 1 cut(s) 488
BspTNI GGTCTC 2 cut(s) 31, 653
BsrBI CCGCTC 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 835
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 835
BssMI GATC 4 cut(s) 6, 90, 416, 848
BssT1I CCWWGG 4 cut(s) 21, 240, 617, 663
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 167, 455
Bst6I CTCTTC 3 cut(s) 147, 626, 651
BstAPI GCANNNNNTGC 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 51, 261
BstDSI CCRYGG 3 cut(s) 261, 346, 504
BstEII GGTNACC 1 cut(s) 729
BstF5I GGATG 4 cut(s) 115, 226, 784, 831
BstFNI CGCG 4 cut(s) 209, 227, 263, 506
BstH2I RGCGCY 1 cut(s) 723
BstHHI GCGC 2 cut(s) 227, 722
BstKTI GATC 4 cut(s) 9, 93, 419, 851
BstMAI GTCTC 3 cut(s) 31, 653, 744
BstMBI GATC 4 cut(s) 6, 90, 416, 848
BstMCI CGRYCG 3 cut(s) 346, 419, 547
BstMWI GCNNNNNNNGC 7 cut(s) 59, 76, 327, 503, 546, 810, 813
BstNI CCWGG 1 cut(s) 106
BstNSI RCATGY 2 cut(s) 83, 325
BstPAI GACNNNNGTC 1 cut(s) 130
BstPI GGTNACC 1 cut(s) 729
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 261
BstUI CGCG 4 cut(s) 209, 227, 263, 506
BstV1I GCAGC 4 cut(s) 314, 484, 813, 825
BstZI CGGCCG 2 cut(s) 343, 544
BsuI GTATCC 1 cut(s) 443
BsuRI GGCC 6 cut(s) 259, 345, 437, 546, 615, 668
BtgI CCRYGG 3 cut(s) 261, 346, 504
BtsCI GGATG 4 cut(s) 115, 226, 784, 831
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 2 cut(s) 51, 261
CciI TCATGA 1 cut(s) 712
CciNI GCGGCCGC 1 cut(s) 544
CfoI GCGC 2 cut(s) 227, 722
Cfr10I RCCGGY 1 cut(s) 835
Cfr13I GGNCC 4 cut(s) 125, 257, 258, 614
Cfr42I CCGCGG 2 cut(s) 264, 507
CseI GACGC 2 cut(s) 193, 198
Csp6I GTAC 2 cut(s) 163, 521
CviAII CATG 3 cut(s) 80, 322, 713
CviQI GTAC 2 cut(s) 163, 521
DpnI GATC 4 cut(s) 8, 92, 418, 850
DpnII GATC 4 cut(s) 6, 90, 416, 848
DrdI GACNNNNNNGTC 1 cut(s) 428
DseDI GACNNNNNNGTC 1 cut(s) 428
EaeI YGGCCR 2 cut(s) 343, 544
EagI CGGCCG 2 cut(s) 343, 544
Eam1104I CTCTTC 3 cut(s) 147, 626, 651
EarI CTCTTC 3 cut(s) 147, 626, 651
EciI GGCGGA 3 cut(s) 281, 562, 637
Ecl136II GAGCTC 1 cut(s) 793
EclXI CGGCCG 2 cut(s) 343, 544
Eco130I CCWWGG 4 cut(s) 21, 240, 617, 663
Eco24I GRGCYC 2 cut(s) 261, 795
Eco31I GGTCTC 2 cut(s) 31, 653
Eco47I GGWCC 1 cut(s) 125
Eco47III AGCGCT 1 cut(s) 721
Eco52I CGGCCG 2 cut(s) 343, 544
Eco53kI GAGCTC 1 cut(s) 793
Eco91I GGTNACC 1 cut(s) 729
EcoICRI GAGCTC 1 cut(s) 793
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 729
EcoRI GAATTC 1 cut(s) 758
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 4 cut(s) 21, 240, 617, 663
EcoT38I GRGCYC 2 cut(s) 261, 795
ErhI CCWWGG 4 cut(s) 21, 240, 617, 663
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 3 cut(s) 83, 325, 716
FaiI YATR 5 cut(s) 81, 323, 482, 714, 768
FaqI GGGAC 3 cut(s) 138, 391, 844
FatI CATG 3 cut(s) 79, 321, 712
FauI CCCGC 2 cut(s) 268, 506
FokI GGATG 4 cut(s) 122, 233, 791, 838
FriOI GRGCYC 2 cut(s) 261, 795
FspBI CTAG 1 cut(s) 693
GlaI GCGC 2 cut(s) 226, 721
GsuI CTGGAG 1 cut(s) 39
HaeII RGCGCY 1 cut(s) 723
HaeIII GGCC 6 cut(s) 259, 345, 437, 546, 615, 668
HapII CCGG 4 cut(s) 97, 128, 550, 836
HgaI GACGC 2 cut(s) 193, 198
HhaI GCGC 2 cut(s) 227, 722
Hin1II CATG 3 cut(s) 83, 325, 716
Hin6I GCGC 2 cut(s) 225, 720
HinP1I GCGC 2 cut(s) 225, 720
HinfI GANTC 5 cut(s) 131, 182, 383, 569, 625
HpaII CCGG 4 cut(s) 97, 128, 550, 836
HphI GGTGA 8 cut(s) 34, 191, 392, 520, 571, 686, 723, 850
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 4 cut(s) 136, 191, 421, 630
Hpy188III TCNNGA 5 cut(s) 10, 56, 199, 606, 713
Hpy8I GTNNAC 1 cut(s) 42
Hpy99I CGWCG 6 cut(s) 200, 209, 214, 308, 776, 788
HpyAV CCTTC 8 cut(s) 44, 248, 289, 397, 487, 550, 559, 835
HpyCH4III ACNGT 2 cut(s) 167, 455
HpyCH4V TGCA 3 cut(s) 393, 426, 749
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 76, 327, 503, 546, 810, 813
Hsp92II CATG 3 cut(s) 83, 325, 716
HspAI GCGC 2 cut(s) 225, 720
KspI CCGCGG 2 cut(s) 264, 507
Kzo9I GATC 4 cut(s) 6, 90, 416, 848
LmnI GCTCC 5 cut(s) 58, 67, 171, 191, 318
Lsp1109I GCAGC 4 cut(s) 314, 484, 813, 825
MaeI CTAG 1 cut(s) 693
MaeIII GTNAC 1 cut(s) 729
MalI GATC 4 cut(s) 8, 92, 418, 850
MbiI CCGCTC 1 cut(s) 513
MboI GATC 4 cut(s) 6, 90, 416, 848
MboII GAAGA 8 cut(s) 25, 164, 477, 502, 565, 574, 643, 668
MfeI CAATTG 2 cut(s) 289, 744
MhlI GDGCHC 2 cut(s) 261, 795
MluCI AATT 4 cut(s) 289, 678, 744, 758
MlyI GAGTC 3 cut(s) 140, 392, 634
MmeI TCCRAC 1 cut(s) 608
MspA1I CMGCKG 4 cut(s) 263, 506, 526, 804
MspI CCGG 4 cut(s) 97, 128, 550, 836
MspR9I CCNGG 1 cut(s) 106
MunI CAATTG 2 cut(s) 289, 744
MvaI CCWGG 1 cut(s) 106
MvnI CGCG 4 cut(s) 209, 227, 263, 506
MwoI GCNNNNNNNGC 7 cut(s) 59, 76, 327, 503, 546, 810, 813
NdeII GATC 4 cut(s) 6, 90, 416, 848
NlaIII CATG 3 cut(s) 83, 325, 716
NlaIV GGNNCC 4 cut(s) 101, 126, 193, 259
NmeAIII GCCGAG 2 cut(s) 88, 694
NmuCI GTSAC 1 cut(s) 729
NotI GCGGCCGC 1 cut(s) 544
NspI RCATGY 2 cut(s) 83, 325
NspV TTCGAA 1 cut(s) 488
PagI TCATGA 1 cut(s) 712
PasI CCCWGGG 1 cut(s) 105
PcsI WCGNNNNNNNCGW 3 cut(s) 195, 768, 780
PfeI GAWTC 2 cut(s) 182, 569
Ple19I CGATCG 1 cut(s) 419
PleI GAGTC 3 cut(s) 139, 391, 633
PpsI GAGTC 3 cut(s) 139, 391, 633
PshAI GACNNNNGTC 1 cut(s) 130
Psp124BI GAGCTC 1 cut(s) 795
Psp6I CCWGG 1 cut(s) 104
PspEI GGTNACC 1 cut(s) 729
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 4 cut(s) 101, 126, 193, 259
PspOMI GGGCCC 1 cut(s) 257
PspPI GGNCC 4 cut(s) 125, 257, 258, 614
PvuI CGATCG 1 cut(s) 419
PvuII CAGCTG 1 cut(s) 526
RsaI GTAC 2 cut(s) 164, 522
RsaNI GTAC 2 cut(s) 163, 521
SacI GAGCTC 1 cut(s) 795
SacII CCGCGG 2 cut(s) 264, 507
Sau3AI GATC 4 cut(s) 6, 90, 416, 848
Sau96I GGNCC 4 cut(s) 125, 257, 258, 614
SchI GAGTC 3 cut(s) 140, 392, 634
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 2 cut(s) 261, 795
SfcI CTRYAG 1 cut(s) 454
Sfr303I CCGCGG 2 cut(s) 264, 507
SfuI TTCGAA 1 cut(s) 488
SgrBI CCGCGG 2 cut(s) 264, 507
SinI GGWCC 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 4 cut(s) 289, 678, 744, 758
SspMI CTAG 1 cut(s) 693
SstI GAGCTC 1 cut(s) 795
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 4 cut(s) 21, 240, 617, 663
TaaI ACNGT 2 cut(s) 167, 455
TaqI TCGA 5 cut(s) 150, 198, 415, 488, 762
TaqII GACCGA 1 cut(s) 717
TasI AATT 4 cut(s) 289, 678, 744, 758
TauI GCSGC 8 cut(s) 266, 503, 506, 543, 546, 549, 642, 807
TfiI GAWTC 2 cut(s) 182, 569
TscAI CASTG 1 cut(s) 146
TseFI GTSAC 1 cut(s) 729
TseI GCWGC 4 cut(s) 327, 497, 801, 813
Tsp45I GTSAC 1 cut(s) 729
TspDTI ATGAA 2 cut(s) 263, 267
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 1 cut(s) 758
XceI RCATGY 2 cut(s) 83, 325
XspI CTAG 1 cut(s) 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.