Rroxscaffold_4G00299330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003426
Physical Location & Seq
Forward (+)
19249797 .. 19255974
6178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299330.1

Sequence Viewer

Length: 555 bp
ATGGTGAGGATTCGTAAGAACCCTCGGGTCTCACCTTGTTTTTCTGCTCCAACGCAGCTGCGGAAGACTTCGGCCACATATCTGCCGTCCGAATCGGTCTTCGTGGGACCTCAATCCCTCTCTAGAGAACGATGGAAAAGGAAGTGTAGAAAGGAAGTGAAGGATGGGTACTCATTTTGCAATCACGACCTGATCCTTATTAGGTCTTACCATAATACCGGCAGCAACGGTGCTACTAGCAATGCTCACAAGTCCTTTTCTTTCAAGAAGCCCAAGCTGACCGCGAGAAAGGCTATTGAGGCCAAGGCGAAAAATGGCTCATCCTCGAAGTCTAGTAACTATATATACTATTTGCTTTTTAGTCCATGGTGGAATCGGAATAGAAGGACTATAGTTGAGGAACAGATCGCGAAGAATATTGTTGTTGAAAATGGTGCTACTACATCCTCTACTAGTGTAGCTACTCCAAATCAAGTGTTAAGGGTAAAAGTAATGTATTACGATCGTTCGGTGTGTTTTTTTGTTACAAAAGATAATGATCATAATGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

21.03

Weight (kDa)

10.18

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 284, 410
AciI CCGC 2 cut(s) 61, 282
AclWI GGATC 1 cut(s) 187
AcoI YGGCCR 1 cut(s) 72
AfaI GTAC 1 cut(s) 170
AgsI TTSAA 2 cut(s) 265, 428
AhlI ACTAGT 1 cut(s) 452
AluBI AGCT 3 cut(s) 58, 277, 461
AluI AGCT 3 cut(s) 58, 277, 461
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 24
AoxI GGCC 2 cut(s) 72, 300
ApeKI GCWGC 3 cut(s) 55, 58, 222
AspS9I GGNCC 1 cut(s) 107
AsuHPI GGTGA 2 cut(s) 16, 24
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 152, 184
BbsI GAAGAC 2 cut(s) 71, 91
BbvI GCAGC 3 cut(s) 45, 67, 234
BccI CCATC 2 cut(s) 126, 158
BceAI ACGGC 1 cut(s) 70
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 34
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 4 cut(s) 123, 237, 333, 453
BfmI CTRYAG 1 cut(s) 390
BisI GCNGC 3 cut(s) 56, 59, 223
BlsI GCNGC 3 cut(s) 57, 60, 224
Bme18I GGWCC 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 108
BpiI GAAGAC 2 cut(s) 71, 91
BsaBI GATNNNNATC 1 cut(s) 537
BsaI GGTCTC 1 cut(s) 34
BsaJI CCNNGG 3 cut(s) 23, 303, 365
Bse118I RCCGGY 1 cut(s) 218
Bse3DI GCAATG 1 cut(s) 247
Bse8I GATNNNNATC 1 cut(s) 537
BseDI CCNNGG 3 cut(s) 23, 303, 365
BseGI GGATG 3 cut(s) 169, 320, 443
BseJI GATNNNNATC 1 cut(s) 537
BseMI GCAATG 1 cut(s) 247
BseXI GCAGC 3 cut(s) 45, 67, 234
Bsh1236I CGCG 2 cut(s) 284, 410
Bsh1285I CGRYCG 1 cut(s) 505
BshFI GGCC 2 cut(s) 74, 302
BsiEI CGRYCG 1 cut(s) 505
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 1 cut(s) 219
BslFI GGGAC 1 cut(s) 120
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 120
BsnI GGCC 2 cut(s) 74, 302
Bso31I GGTCTC 1 cut(s) 34
BsoBI CYCGRG 1 cut(s) 24
Bsp143I GATC 4 cut(s) 192, 405, 502, 538
Bsp19I CCATGG 1 cut(s) 365
Bsp68I TCGCGA 1 cut(s) 410
BspACI CCGC 2 cut(s) 61, 282
BspANI GGCC 2 cut(s) 74, 302
BspFNI CGCG 2 cut(s) 284, 410
BspLI GGNNCC 1 cut(s) 108
BspPI GGATC 1 cut(s) 187
BspTNI GGTCTC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 3 cut(s) 23, 303, 365
BssMI GATC 4 cut(s) 192, 405, 502, 538
BssT1I CCWWGG 2 cut(s) 303, 365
Bst4CI ACNGT 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 365
BstF5I GGATG 3 cut(s) 169, 320, 443
BstFNI CGCG 2 cut(s) 284, 410
BstKTI GATC 4 cut(s) 195, 408, 505, 541
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 4 cut(s) 192, 405, 502, 538
BstMCI CGRYCG 1 cut(s) 505
BstMWI GCNNNNNNNGC 2 cut(s) 290, 299
BstSFI CTRYAG 1 cut(s) 390
BstUI CGCG 2 cut(s) 284, 410
BstV1I GCAGC 3 cut(s) 45, 67, 234
BstV2I GAAGAC 2 cut(s) 71, 91
BsuRI GGCC 2 cut(s) 74, 302
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 3 cut(s) 169, 320, 443
BtuMI TCGCGA 1 cut(s) 410
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 169
CviAII CATG 1 cut(s) 366
CviJI RGCY 8 cut(s) 58, 74, 271, 277, 293, 302, 318, 461
CviKI_1 RGCY 8 cut(s) 58, 74, 271, 277, 293, 302, 318, 461
CviQI GTAC 1 cut(s) 169
DpnI GATC 4 cut(s) 194, 407, 504, 540
DpnII GATC 4 cut(s) 192, 405, 502, 538
EaeI YGGCCR 1 cut(s) 72
Eco130I CCWWGG 2 cut(s) 303, 365
Eco31I GGTCTC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 24
EcoO109I RGGNCCY 1 cut(s) 107
EcoT14I CCWWGG 2 cut(s) 303, 365
ErhI CCWWGG 2 cut(s) 303, 365
FaeI CATG 1 cut(s) 369
FaiI YATR 8 cut(s) 79, 213, 342, 344, 346, 367, 392, 543
FaqI GGGAC 1 cut(s) 120
FatI CATG 1 cut(s) 365
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 3 cut(s) 56, 59, 223
FokI GGATG 3 cut(s) 176, 307, 430
Fsp4HI GCNGC 3 cut(s) 56, 59, 223
FspBI CTAG 4 cut(s) 123, 237, 333, 453
GluI GCNGC 3 cut(s) 56, 59, 223
HaeIII GGCC 2 cut(s) 74, 302
HapII CCGG 1 cut(s) 219
Hin1II CATG 1 cut(s) 369
HinfI GANTC 3 cut(s) 10, 92, 373
HpaII CCGG 1 cut(s) 219
HphI GGTGA 2 cut(s) 16, 24
Hpy188I TCNGA 2 cut(s) 91, 378
Hpy188III TCNNGA 4 cut(s) 123, 185, 265, 409
HpyAV CCTTC 2 cut(s) 154, 378
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4V TGCA 1 cut(s) 180
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 299
Hsp92II CATG 1 cut(s) 369
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 4 cut(s) 192, 405, 502, 538
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 2 cut(s) 203, 232
Lsp1109I GCAGC 3 cut(s) 45, 67, 234
MaeI CTAG 4 cut(s) 123, 237, 333, 453
MaeIII GTNAC 2 cut(s) 335, 523
MalI GATC 4 cut(s) 194, 407, 504, 540
MboI GATC 4 cut(s) 192, 405, 502, 538
MboII GAAGA 3 cut(s) 76, 91, 424
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 7 cut(s) 33, 120, 128, 292, 334, 391, 457
MseI TTAA 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 1 cut(s) 219
MvnI CGCG 2 cut(s) 284, 410
MwoI GCNNNNNNNGC 2 cut(s) 290, 299
NcoI CCATGG 1 cut(s) 365
NdeII GATC 4 cut(s) 192, 405, 502, 538
NlaIII CATG 1 cut(s) 369
NlaIV GGNNCC 1 cut(s) 108
NruI TCGCGA 1 cut(s) 410
PfeI GAWTC 3 cut(s) 10, 92, 373
PkrI GCNGC 3 cut(s) 57, 60, 224
Ple19I CGATCG 1 cut(s) 505
PpuMI RGGWCCY 1 cut(s) 107
Psp5II RGGWCCY 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 1 cut(s) 107
PspPPI RGGWCCY 1 cut(s) 107
PvuI CGATCG 1 cut(s) 505
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 410
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 3 cut(s) 56, 59, 223
Sau3AI GATC 4 cut(s) 192, 405, 502, 538
Sau96I GGNCC 1 cut(s) 107
SetI ASST 7 cut(s) 37, 60, 112, 192, 206, 279, 463
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 452
SsiI CCGC 2 cut(s) 61, 282
SspI AATATT 1 cut(s) 418
SspMI CTAG 4 cut(s) 123, 237, 333, 453
StyI CCWWGG 2 cut(s) 303, 365
TaaI ACNGT 1 cut(s) 230
TaqI TCGA 1 cut(s) 326
TaqII GACCGA 1 cut(s) 85
TfiI GAWTC 3 cut(s) 10, 92, 373
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TseI GCWGC 3 cut(s) 55, 58, 222
VpaK11BI GGWCC 1 cut(s) 107
XbaI TCTAGA 1 cut(s) 122
XspI CTAG 4 cut(s) 123, 237, 333, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.