Rorug01G0251500

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001223
Physical Location & Seq
Forward (+)
35943343 .. 35948432
5090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0251500.1

Sequence Viewer

Length: 2097 bp
ATGGGAGCTGATATGTGGGAGAGTTTCAATAATCCTACTGACTCACTTCTGCCAAGCATGTTGATGGGATATGATAGTGGTTCTGGAAAACAGAATTTCTTGACATCTTGGAAAAGTGAGAATGATCCATCACCAGGGATATTCTTGGTTGGACTTTCAGCAGAGGTGCCACCACAAGTGTTCATTTGGATTAATGGATCTACTCCCCACTGGAGAAATGGGCCATGGGATACATCAAGGTTTATTGGTGTACCCACAAGGAAATCTCAGTATATTAATCCATTTACTCTTGTTGATAATGTGACACAGGGAACAAGGTATTTTTCTTACGGTTTTAACAAAATAGGTGACAAAGTTCTTGCATATTTCGACATATCTTCCGAAGGAAAACTGAGCTTTTTGTTTTCAGAAAGTGGCAAGAACTGGCGTCTTAACTGGGAGTCATGGACAAACCCATGCGATAATTATGGCGCCTGTGGACCTTTTGGGGTTTGCATAGCTTCTGACTCTCCAATCTGTAAGTGTTTGAAAGGGTTTATACCAAAGTCAAATGAAGAGTGGAGCAAAAGAAACTGGACTGGAGGTTGTGTGAGACGAACAGATTTGTCTTGTGAGACACACACAAATGAATCAGTCTCATCATCAAACAGAAAAGATGGGTTTTTAAAGTTGGAGAGATCGAAAGTACCCGATTTTCATCAATATTTGACTTCTTTGGCTCTAAACAGTTTTGAGGACTGCAAGATAAAGTGCCTGAGTAATTGTTCTTGCCTAGCTTATGCTTATGTTGATATCCTAGGGTGTTTGGTCTGGTACAAAGAACTTATTGATATTCAGCAGTTTCCATCTCTTGGAGAAGATCTTTATATCCGCCTAGCAGACTCAGAACTAGGTGAAGGAAAACCAGTAAAGTTAATTGCCAGCGTTACAGCTATTGGATTTATGATGATCATCTTGGTGGCTATAGTCTTCAGTTTGCACCGGTGGCATGCTAATCAAAAGCGACATGTCAAATTAACAACAAGGCGTTTGGAATCAGCTAATTTGATTGAAATTTATAGAGACGATCTTCAAGAATATATGGAAAAACATAATCTGTCGGAGCTAAAGATATATGATTTTGATAGCATATTAATAGCCACAGAAAACTTCAGCATCAGAAACAAACTCGGGCAAGGAGGCTTTGGCCCAGTTTATAAGGGGATGCTACCAGAAGGGAAGGAAATAGCTGTGAAGAGACTATCTAGTAGCTCAGGACAAGGTGTTGAAGAGTTCAAGAATGAGATGCTATTGATCTCCATTCTTCAACACAAAAATCTTGTTAGGATCATGGGTTATTGTGTTAAAGAGAATGAGAAGTTACTTATTTACGAATTCATGCCTAACAAAAGCTTGGATACTTTCCTATACGATTTAAGGAAGAGAGCAGTGCTTGATTGGACTACACGCTTTAATATTATTCAAGGTGTTGCTAAAGGTCTTCTTTACCTCCATCATGATTCCTATGTGAAGGTGATTCATAGAGATCTAAAAGTCAGTAACATCCTCTTGGATGAGAGAATGAATCCAAAAATTTCAGATTTTGGATTAGCACGCATAGTTGAAGGAACACAGTGTCTAGAAAATACTCAGAAGCTTGTGGGAACACGTGGCTATATATCTCCGGAGTATGCTATGAGTGGGATATTTTCTGAAAAATCTGATATCTACAGCTTTGGGGTCTTGGTATTGGAGATTGTTAGCAGCAAGAAGAACAATAGCTTCTATTTATATGACCAACAACTTGGCTTCCTTGCCTATGCTTGGAACTTGTGGAATGAAGGCAAGGCATTGGAGTTAGTAGATGAAGTTTTGGGTGATTCATACTCCTCATCAGAAGTTATGACTTGCATGCATGTTGGGCTTCTTTGTGTACAGGACAATGCTACAGATAGACCAACAATGGCGGATGTAACTTTAATGCTAAACAGTGAGAAAGATGGTCCACATCCAAAGAAACCTGTATTCACTATCCAAAACTCCGTTAATCAACCATCCGATGAAAATATTAATTCCTCCAAAAATGAAGCCAGCATTACAATGATCGAAGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

698

Amino Acids

79.04

Weight (kDa)

5.99

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 5 - 38 1.4e-06 D-mannose binding lectin
S_locus_glycop PF00954 71 - 183 5.2e-27 S-locus glycoprotein domain
PAN_2 PF08276 216 - 278 6.9e-17 PAN-like domain
Pkinase PF00069 385 - 584 2e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 386 - 652 2e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1197
AccB1I GGYRCC 2 cut(s) 166, 470
AccB7I CCANNNNNTGG 1 cut(s) 851
AccIII TCCGGA 1 cut(s) 1663
AciI CCGC 2 cut(s) 871, 1946
AclWI GGATC 3 cut(s) 119, 205, 1334
AcsI RAATTY 4 cut(s) 94, 1053, 1373, 1572
AcuI CTGAAG 2 cut(s) 955, 1135
AcvI CACGTG 1 cut(s) 1649
AcyI GRCGYC 2 cut(s) 427, 471
AfaI GTAC 4 cut(s) 252, 687, 815, 1914
AfiI CCNNNNNNNGG 3 cut(s) 134, 851, 1803
AflIII ACRYGT 2 cut(s) 1006, 1646
AgeI ACCGGT 1 cut(s) 981
AgsI TTSAA 9 cut(s) 28, 529, 1052, 1073, 1268, 1276, 1307, 1463, 1604
AjnI CCWGG 1 cut(s) 133
Alw26I GTCTC 5 cut(s) 586, 608, 640, 1056, 1231
AlwI GGATC 3 cut(s) 119, 205, 1334
Ama87I CYCGRG 1 cut(s) 1169
Aor13HI TCCGGA 1 cut(s) 1663
AoxI GGCC 2 cut(s) 221, 1186
ApeKI GCWGC 1 cut(s) 1743
ApoI RAATTY 4 cut(s) 94, 1053, 1373, 1572
AseI ATTAAT 4 cut(s) 192, 276, 1133, 2049
AsiGI ACCGGT 1 cut(s) 981
AspA2I CCTAGG 1 cut(s) 796
AspLEI GCGC 1 cut(s) 473
AspS9I GGNCC 4 cut(s) 221, 479, 1187, 1982
AsuHPI GGTGA 5 cut(s) 123, 359, 905, 1525, 1868
AvaI CYCGRG 1 cut(s) 1169
AvaII GGWCC 2 cut(s) 479, 1982
AvrII CCTAGG 1 cut(s) 796
BanI GGYRCC 2 cut(s) 166, 470
BbrPI CACGTG 1 cut(s) 1649
BbsI GAAGAC 2 cut(s) 961, 1472
BbvI GCAGC 1 cut(s) 1755
BccI CCATC 7 cut(s) 58, 136, 650, 853, 1500, 1973, 2041
BciT130I CCWGG 1 cut(s) 135
BciVI GTATCC 2 cut(s) 223, 1390
BclI TGATCA 1 cut(s) 948
BcoDI GTCTC 5 cut(s) 586, 608, 640, 1056, 1231
BfaI CTAG 6 cut(s) 773, 797, 875, 890, 1245, 1619
BfmI CTRYAG 3 cut(s) 963, 1708, 1926
BfoI RGCGCY 1 cut(s) 474
BfuI GTATCC 2 cut(s) 223, 1390
BglII AGATCT 2 cut(s) 859, 1525
BisI GCNGC 1 cut(s) 1744
BlnI CCTAGG 1 cut(s) 796
BlsI GCNGC 1 cut(s) 1745
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 2 cut(s) 479, 1982
BmeT110I CYCGRG 1 cut(s) 1169
BmgT120I GGNCC 4 cut(s) 221, 479, 1187, 1982
BmiI GGNNCC 2 cut(s) 168, 472
BmrFI CCNGG 1 cut(s) 135
BmrI ACTGGG 2 cut(s) 445, 1184
BmsI GCATC 3 cut(s) 1164, 1194, 1275
BmuI ACTGGG 2 cut(s) 445, 1184
BpiI GAAGAC 2 cut(s) 961, 1472
BpmI CTGGAG 2 cut(s) 232, 600
Bpu10I CCTNAGC 1 cut(s) 1252
BsaAI YACGTR 1 cut(s) 1649
BsaBI GATNNNNATC 2 cut(s) 696, 950
BsaHI GRCGYC 2 cut(s) 427, 471
BsaJI CCNNGG 3 cut(s) 134, 224, 796
BsaWI WCCGGW 2 cut(s) 981, 1663
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 851, 1803
Bse118I RCCGGY 1 cut(s) 981
Bse1I ACTGG 7 cut(s) 215, 428, 440, 578, 583, 905, 1190
Bse8I GATNNNNATC 2 cut(s) 696, 950
BseAI TCCGGA 1 cut(s) 1663
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 3 cut(s) 134, 224, 796
BseGI GGATG 6 cut(s) 1209, 1542, 1558, 1954, 1987, 2033
BseJI GATNNNNATC 2 cut(s) 696, 950
BseLI CCNNNNNNNGG 3 cut(s) 134, 851, 1803
BseMII CTCAG 6 cut(s) 281, 383, 746, 897, 1266, 1643
BseNI ACTGG 7 cut(s) 215, 428, 440, 578, 583, 905, 1190
BseRI GAGGAG 1 cut(s) 1858
BseXI GCAGC 1 cut(s) 1755
BshFI GGCC 2 cut(s) 223, 1188
BshNI GGYRCC 2 cut(s) 166, 470
BshTI ACCGGT 1 cut(s) 981
BsiHKCI CYCGRG 1 cut(s) 1169
BsiSI CCGG 2 cut(s) 982, 1664
BslI CCNNNNNNNGG 3 cut(s) 134, 851, 1803
BsmAI GTCTC 5 cut(s) 586, 608, 640, 1056, 1231
BsmBI CGTCTC 2 cut(s) 586, 1056
BsnI GGCC 2 cut(s) 223, 1188
BsoBI CYCGRG 1 cut(s) 1169
Bsp13I TCCGGA 1 cut(s) 1663
Bsp1407I TGTACA 1 cut(s) 1912
Bsp19I CCATGG 1 cut(s) 224
BspACI CCGC 2 cut(s) 871, 1946
BspANI GGCC 2 cut(s) 223, 1188
BspCNI CTCAG 6 cut(s) 280, 384, 747, 896, 1265, 1642
BspEI TCCGGA 1 cut(s) 1663
BspHI TCATGA 1 cut(s) 1495
BspLI GGNNCC 2 cut(s) 168, 472
BspPI GGATC 3 cut(s) 119, 205, 1334
BspT107I GGYRCC 2 cut(s) 166, 470
BsrFI RCCGGY 1 cut(s) 981
BsrGI TGTACA 1 cut(s) 1912
BsrI ACTGG 7 cut(s) 215, 428, 440, 578, 583, 905, 1190
BssAI RCCGGY 1 cut(s) 981
BssECI CCNNGG 3 cut(s) 134, 224, 796
BssNI GRCGYC 2 cut(s) 427, 471
BssT1I CCWWGG 2 cut(s) 224, 796
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 4 cut(s) 332, 728, 1614, 1970
Bst6I CTCTTC 4 cut(s) 549, 1229, 1263, 1415
BstACI GRCGYC 2 cut(s) 427, 471
BstAUI TGTACA 1 cut(s) 1912
BstBAI YACGTR 1 cut(s) 1649
BstC8I GCNNGC 5 cut(s) 922, 990, 1594, 1892, 2071
BstDEI CTNAG 6 cut(s) 267, 392, 755, 883, 1252, 1629
BstDSI CCRYGG 1 cut(s) 224
BstF5I GGATG 6 cut(s) 1209, 1542, 1558, 1954, 1987, 2033
BstH2I RGCGCY 1 cut(s) 474
BstHHI GCGC 1 cut(s) 473
BstMAI GTCTC 5 cut(s) 586, 608, 640, 1056, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 985, 1900
BstNI CCWGG 1 cut(s) 135
BstNSI RCATGY 5 cut(s) 61, 992, 1010, 1894, 1898
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 3 cut(s) 963, 1708, 1926
BstV1I GCAGC 1 cut(s) 1755
BstV2I GAAGAC 2 cut(s) 961, 1472
BstX2I RGATCY 3 cut(s) 197, 859, 1525
BstXI CCANNNNNNTGG 1 cut(s) 1784
BstYI RGATCY 3 cut(s) 197, 859, 1525
BsuI GTATCC 2 cut(s) 223, 1390
BsuRI GGCC 2 cut(s) 223, 1188
BtgI CCRYGG 1 cut(s) 224
BtsCI GGATG 6 cut(s) 1209, 1542, 1558, 1954, 1987, 2033
BtsI GCAGTG 1 cut(s) 1434
BtsIMutI CAGTG 4 cut(s) 208, 1434, 1619, 1975
Cac8I GCNNGC 5 cut(s) 922, 990, 1594, 1892, 2071
CciI TCATGA 1 cut(s) 1495
CfoI GCGC 1 cut(s) 473
Cfr10I RCCGGY 1 cut(s) 981
Cfr13I GGNCC 4 cut(s) 221, 479, 1187, 1982
CseI GACGC 1 cut(s) 416
Csp6I GTAC 4 cut(s) 251, 686, 814, 1913
CspAI ACCGGT 1 cut(s) 981
CviQI GTAC 4 cut(s) 251, 686, 814, 1913
DdeI CTNAG 6 cut(s) 267, 392, 755, 883, 1252, 1629
DinI GGCGCC 1 cut(s) 472
DraI TTTAAA 1 cut(s) 666
Eam1104I CTCTTC 4 cut(s) 549, 1229, 1263, 1415
EarI CTCTTC 4 cut(s) 549, 1229, 1263, 1415
EciI GGCGGA 2 cut(s) 860, 1961
Eco130I CCWWGG 2 cut(s) 224, 796
Eco32I GATATC 2 cut(s) 793, 1705
Eco47I GGWCC 2 cut(s) 479, 1982
Eco57I CTGAAG 2 cut(s) 955, 1135
Eco72I CACGTG 1 cut(s) 1649
Eco88I CYCGRG 1 cut(s) 1169
EcoRI GAATTC 1 cut(s) 1373
EcoRII CCWGG 1 cut(s) 133
EcoRV GATATC 2 cut(s) 793, 1705
EcoT14I CCWWGG 2 cut(s) 224, 796
EcoT22I ATGCAT 1 cut(s) 1896
EgeI GGCGCC 1 cut(s) 472
EheI GGCGCC 1 cut(s) 472
ErhI CCWWGG 2 cut(s) 224, 796
Esp3I CGTCTC 2 cut(s) 586, 1056
FalI AAGNNNNNCTT 2 cut(s) 1467, 1499
FbaI TGATCA 1 cut(s) 948
Fnu4HI GCNGC 1 cut(s) 1744
FokI GGATG 6 cut(s) 1216, 1529, 1565, 1961, 1974, 2020
Fsp4HI GCNGC 1 cut(s) 1744
FspBI CTAG 6 cut(s) 773, 797, 875, 890, 1245, 1619
GlaI GCGC 1 cut(s) 472
GluI GCNGC 1 cut(s) 1744
GsuI CTGGAG 2 cut(s) 232, 600
HaeII RGCGCY 1 cut(s) 474
HaeIII GGCC 2 cut(s) 223, 1188
HapII CCGG 2 cut(s) 982, 1664
HgaI GACGC 1 cut(s) 416
HhaI GCGC 1 cut(s) 473
Hin1I GRCGYC 2 cut(s) 427, 471
Hin6I GCGC 1 cut(s) 471
HinP1I GCGC 1 cut(s) 471
HindIII AAGCTT 2 cut(s) 1390, 1634
HpaII CCGG 2 cut(s) 982, 1664
HphI GGTGA 5 cut(s) 123, 359, 905, 1525, 1868
Hpy166II GTNNAC 4 cut(s) 251, 479, 1913, 1985
Hpy188III TCNNGA 8 cut(s) 84, 100, 1073, 1254, 1276, 1496, 1619, 1664
Hpy8I GTNNAC 4 cut(s) 251, 479, 1913, 1985
HpyAV CCTTC 8 cut(s) 377, 890, 1208, 1213, 1504, 1598, 1814, 2081
HpyCH4III ACNGT 4 cut(s) 332, 728, 1614, 1970
HpyCH4IV ACGT 2 cut(s) 1648, 2092
HpyCH4V TGCA 6 cut(s) 362, 495, 741, 979, 1890, 1894
HpyF10VI GCNNNNNNNGC 2 cut(s) 985, 1900
HpyF3I CTNAG 6 cut(s) 267, 392, 755, 883, 1252, 1629
HpySE526I ACGT 2 cut(s) 1648, 2092
Hsp92I GRCGYC 2 cut(s) 427, 471
HspAI GCGC 1 cut(s) 471
KasI GGCGCC 1 cut(s) 470
Kpn2I TCCGGA 1 cut(s) 1663
Ksp22I TGATCA 1 cut(s) 948
LmnI GCTCC 3 cut(s) 5, 561, 1102
Lsp1109I GCAGC 1 cut(s) 1755
LweI GCATC 3 cut(s) 1164, 1194, 1275
MaeI CTAG 6 cut(s) 773, 797, 875, 890, 1245, 1619
MaeII ACGT 2 cut(s) 1648, 2092
MaeIII GTNAC 6 cut(s) 301, 347, 925, 1359, 1538, 1951
MflI RGATCY 3 cut(s) 197, 859, 1525
Mly113I GGCGCC 1 cut(s) 471
MlyI GAGTC 4 cut(s) 35, 449, 500, 875
MmeI TCCRAC 3 cut(s) 130, 651, 1080
MnlI CCTC 8 cut(s) 157, 575, 727, 1172, 1499, 1556, 1879, 2065
Mph1103I ATGCAT 1 cut(s) 1896
MroI TCCGGA 1 cut(s) 1663
MslI CAYNNNNRTG 4 cut(s) 62, 624, 956, 2078
MspI CCGG 2 cut(s) 982, 1664
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 2 cut(s) 985, 1900
NarI GGCGCC 1 cut(s) 471
NcoI CCATGG 1 cut(s) 224
NlaIV GGNNCC 2 cut(s) 168, 472
NmuCI GTSAC 2 cut(s) 301, 347
NsiI ATGCAT 1 cut(s) 1896
NspI RCATGY 5 cut(s) 61, 992, 1010, 1894, 1898
PaeI GCATGC 2 cut(s) 992, 1894
PagI TCATGA 1 cut(s) 1495
PciI ACATGT 1 cut(s) 1006
PfeI GAWTC 6 cut(s) 629, 1034, 1499, 1516, 1564, 1859
PflMI CCANNNNNTGG 1 cut(s) 851
PinAI ACCGGT 1 cut(s) 981
PkrI GCNGC 1 cut(s) 1745
PleI GAGTC 4 cut(s) 35, 448, 500, 875
PluTI GGCGCC 1 cut(s) 474
PmaCI CACGTG 1 cut(s) 1649
PmlI CACGTG 1 cut(s) 1649
PpsI GAGTC 4 cut(s) 35, 448, 500, 875
Ppu21I YACGTR 1 cut(s) 1649
PscI ACATGT 1 cut(s) 1006
PshBI ATTAAT 4 cut(s) 192, 276, 1133, 2049
PsiI TTATAA 1 cut(s) 1197
Psp6I CCWGG 1 cut(s) 133
PspCI CACGTG 1 cut(s) 1649
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 168, 472
PspPI GGNCC 4 cut(s) 221, 479, 1187, 1982
PsuI RGATCY 3 cut(s) 197, 859, 1525
RsaI GTAC 4 cut(s) 252, 687, 815, 1914
RsaNI GTAC 4 cut(s) 251, 686, 814, 1913
RseI CAYNNNNRTG 4 cut(s) 62, 624, 956, 2078
SatI GCNGC 1 cut(s) 1744
Sau96I GGNCC 4 cut(s) 221, 479, 1187, 1982
SchI GAGTC 4 cut(s) 35, 449, 500, 875
ScrFI CCNGG 1 cut(s) 135
SfaNI GCATC 3 cut(s) 1164, 1194, 1275
SfcI CTRYAG 3 cut(s) 963, 1708, 1926
SfoI GGCGCC 1 cut(s) 472
SgrAI CRCCGGYG 1 cut(s) 981
SinI GGWCC 2 cut(s) 479, 1982
SmiMI CAYNNNNRTG 4 cut(s) 62, 624, 956, 2078
SphI GCATGC 2 cut(s) 992, 1894
SsiI CCGC 2 cut(s) 871, 1946
SspDI GGCGCC 1 cut(s) 470
SspI AATATT 3 cut(s) 704, 1456, 2047
SspMI CTAG 6 cut(s) 773, 797, 875, 890, 1245, 1619
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 2 cut(s) 224, 796
TaaI ACNGT 4 cut(s) 332, 728, 1614, 1970
TaiI ACGT 2 cut(s) 1651, 2095
TaqI TCGA 3 cut(s) 369, 680, 2085
TatI WGTACW 1 cut(s) 1912
TfiI GAWTC 6 cut(s) 629, 1034, 1499, 1516, 1564, 1859
TscAI CASTG 4 cut(s) 215, 1434, 1619, 1975
TseFI GTSAC 2 cut(s) 301, 347
TseI GCWGC 1 cut(s) 1743
Tsp45I GTSAC 2 cut(s) 301, 347
TspGWI ACGGA 1 cut(s) 2011
TspRI CASTG 4 cut(s) 215, 1434, 1619, 1975
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 2 cut(s) 479, 1982
VspI ATTAAT 4 cut(s) 192, 276, 1133, 2049
XapI RAATTY 4 cut(s) 94, 1053, 1373, 1572
XbaI TCTAGA 1 cut(s) 1618
XceI RCATGY 5 cut(s) 61, 992, 1010, 1894, 1898
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XmaJI CCTAGG 1 cut(s) 796
XspI CTAG 6 cut(s) 773, 797, 875, 890, 1245, 1619
Zsp2I ATGCAT 1 cut(s) 1896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.