AT4G23470

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001223
Physical Location & Seq
Forward (+)
12248842 .. 12251396
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23470.1

Sequence Viewer

Length: 768 bp
ATGCCGAAACAGGATATGGAGAAGATGGAGCTTCGTAAGAACTTCCGTAATGTTTGGCATACCGATCTTACCCACAGTATCCAGAACGATACTCCTTATTGTTGCTTCGCGTTGTGGTGTGCCCCTTGTGCATCATACTTGCTTCGCAAGCGTGCGCTTTACGATGATATGTCAAGGTACGTCTGCTGTGCCGGTTACATGCCTTGTAGTGGTAGGTGTGGAGAAGCCAAATGTCCTCAACTTTGTCTTGCCACTGAGGTCTTCTGCTGCTTTGCAAACTCTGTGGCCTCAACTCGCTTTCTTCTGCAAGATGAGTTCCAAATTCAAACGACAAAATGTGACAACTGCATCATTGGTTTCATGGTTTGCCTCAGCCAAGTGGCTTGCATATTCTCCATAGTTGCATGTATTGTCGGTATGGATGAGCTTTCAGAAGCTTCTCAGATACTCACATGTTGCTCTGACATGGTGTACTGCACGGTTTGCGCATGTATGCAGACACAACACAAGATGGAAATGGACAAGAGGGACGGTAAGTTCGGGCCACAGCCAATGGCAGTGCCTCCGGCTCAGCAAATGTCACGGTTTGATCAAGCCACCCCACCCGCAGTCGGTTATCCTCCACAACAAGGTTATCCACCTTCTGGTTATCCTCAACACCCTCCACAAGGTTATCCACCTTCTGGCTATCCTCAAAACCCTCCTCCCTCAGCTTATTCTCAATACCCTCCTGGGGCTTATCCTCCTCCTCCCGCTTACCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.43

Weight (kDa)

6.39

Isoelectric Point (pI)

67.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 487
AccB7I CCANNNNNTGG 2 cut(s) 644, 683
AccII CGCG 1 cut(s) 110
AciI CCGC 2 cut(s) 606, 753
AcsI RAATTY 1 cut(s) 321
AfaI GTAC 2 cut(s) 179, 473
AfiI CCNNNNNNNGG 7 cut(s) 209, 611, 629, 644, 668, 683, 733
AflIII ACRYGT 1 cut(s) 452
AgsI TTSAA 1 cut(s) 326
AjnI CCWGG 1 cut(s) 730
AloI GAACNNNNNNTCC 2 cut(s) 521, 553
AluBI AGCT 4 cut(s) 31, 427, 437, 713
AluI AGCT 4 cut(s) 31, 427, 437, 713
AoxI GGCC 2 cut(s) 285, 542
ApeKI GCWGC 1 cut(s) 267
ApoI RAATTY 1 cut(s) 321
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
AspLEI GCGC 2 cut(s) 157, 488
AspS9I GGNCC 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 253
BbvCI CCTCAGC 2 cut(s) 371, 709
BbvI GCAGC 1 cut(s) 254
BccI CCATC 2 cut(s) 19, 505
BciT130I CCWGG 1 cut(s) 732
BciVI GTATCC 1 cut(s) 89
BclI TGATCA 1 cut(s) 589
BfuI GTATCC 1 cut(s) 89
BisI GCNGC 1 cut(s) 268
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 1 cut(s) 269
Bme1390I CCNGG 1 cut(s) 732
BmgT120I GGNCC 1 cut(s) 542
BmrFI CCNGG 1 cut(s) 732
BmsI GCATC 2 cut(s) 140, 357
BpiI GAAGAC 1 cut(s) 253
Bpu10I CCTNAGC 2 cut(s) 371, 709
Bpu1102I GCTNAGC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 731
Bsc4I CCNNNNNNNGG 7 cut(s) 209, 611, 629, 644, 668, 683, 733
Bse118I RCCGGY 1 cut(s) 191
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 7 cut(s) 209, 611, 629, 644, 668, 683, 733
BseMII CTCAG 5 cut(s) 246, 385, 455, 584, 723
BseRI GAGGAG 3 cut(s) 693, 735, 738
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 460
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 2 cut(s) 287, 544
BsiSI CCGG 2 cut(s) 192, 566
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 7 cut(s) 209, 611, 629, 644, 668, 683, 733
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 2 cut(s) 287, 544
Bsp1286I GDGCHC 1 cut(s) 124
Bsp143I GATC 2 cut(s) 64, 589
Bsp1720I GCTNAGC 1 cut(s) 570
BspACI CCGC 2 cut(s) 606, 753
BspANI GGCC 2 cut(s) 287, 544
BspCNI CTCAG 5 cut(s) 247, 384, 454, 583, 722
BspFNI CGCG 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 731
BssMI GATC 2 cut(s) 64, 589
Bst2UI CCWGG 1 cut(s) 732
Bst4CI ACNGT 4 cut(s) 77, 481, 533, 585
BstAPI GCANNNNNTGC 1 cut(s) 483
BstC8I GCNNGC 3 cut(s) 149, 153, 385
BstDEI CTNAG 5 cut(s) 255, 371, 441, 570, 709
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 110
BstHHI GCGC 2 cut(s) 157, 488
BstKTI GATC 2 cut(s) 67, 592
BstMBI GATC 2 cut(s) 64, 589
BstMWI GCNNNNNNNGC 3 cut(s) 128, 148, 483
BstNI CCWGG 1 cut(s) 732
BstNSI RCATGY 4 cut(s) 202, 408, 456, 492
BstSCI CCNGG 1 cut(s) 730
BstSLI GKGCMC 1 cut(s) 124
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 1 cut(s) 254
BstV2I GAAGAC 1 cut(s) 253
BsuI GTATCC 1 cut(s) 89
BsuRI GGCC 2 cut(s) 287, 544
BtsCI GGATG 1 cut(s) 427
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 2 cut(s) 252, 564
Cac8I GCNNGC 3 cut(s) 149, 153, 385
CfoI GCGC 2 cut(s) 157, 488
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 1 cut(s) 542
Csp6I GTAC 2 cut(s) 178, 472
CspCI CAANNNNNGTGG 2 cut(s) 264, 299
CviAII CATG 6 cut(s) 199, 361, 405, 453, 466, 489
CviQI GTAC 2 cut(s) 178, 472
DdeI CTNAG 5 cut(s) 255, 371, 441, 570, 709
DpnI GATC 2 cut(s) 66, 591
DpnII GATC 2 cut(s) 64, 589
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoRII CCWGG 1 cut(s) 730
FaeI CATG 6 cut(s) 202, 364, 408, 456, 469, 492
FaqI GGGAC 1 cut(s) 542
FatI CATG 6 cut(s) 198, 360, 404, 452, 465, 488
FauI CCCGC 2 cut(s) 613, 760
FbaI TGATCA 1 cut(s) 589
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 1 cut(s) 268
FspI TGCGCA 1 cut(s) 487
GlaI GCGC 2 cut(s) 156, 487
GluI GCNGC 1 cut(s) 268
HaeIII GGCC 2 cut(s) 287, 544
HapII CCGG 2 cut(s) 192, 566
HhaI GCGC 2 cut(s) 157, 488
Hin1II CATG 6 cut(s) 202, 364, 408, 456, 469, 492
Hin6I GCGC 2 cut(s) 155, 486
HinP1I GCGC 2 cut(s) 155, 486
HindIII AAGCTT 1 cut(s) 435
HpaII CCGG 2 cut(s) 192, 566
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 3 cut(s) 433, 444, 463
Hpy188III TCNNGA 1 cut(s) 82
Hpy8I GTNNAC 1 cut(s) 472
HpyAV CCTTC 2 cut(s) 651, 690
HpyCH4III ACNGT 4 cut(s) 77, 481, 533, 585
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 8 cut(s) 131, 275, 307, 348, 387, 404, 477, 496
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 148, 483
HpyF3I CTNAG 5 cut(s) 255, 371, 441, 570, 709
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 6 cut(s) 202, 364, 408, 456, 469, 492
HspAI GCGC 2 cut(s) 155, 486
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 2 cut(s) 64, 589
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 7 cut(s) 95, 205, 579, 630, 669, 717, 744
Lsp1109I GCAGC 1 cut(s) 254
LweI GCATC 2 cut(s) 140, 357
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 3 cut(s) 194, 338, 579
MalI GATC 2 cut(s) 66, 591
MboI GATC 2 cut(s) 64, 589
MboII GAAGA 3 cut(s) 34, 253, 293
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 1 cut(s) 321
MspI CCGG 2 cut(s) 192, 566
MspR9I CCNGG 1 cut(s) 732
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 3 cut(s) 128, 148, 483
NdeII GATC 2 cut(s) 64, 589
NlaIII CATG 6 cut(s) 202, 364, 408, 456, 469, 492
NmuCI GTSAC 2 cut(s) 338, 579
NsbI TGCGCA 1 cut(s) 487
NspI RCATGY 4 cut(s) 202, 408, 456, 492
PciI ACATGT 1 cut(s) 452
PflMI CCANNNNNTGG 2 cut(s) 644, 683
PkrI GCNGC 1 cut(s) 269
PscI ACATGT 1 cut(s) 452
Psp6I CCWGG 1 cut(s) 730
PspGI CCWGG 1 cut(s) 730
PspPI GGNCC 1 cut(s) 542
RsaI GTAC 2 cut(s) 179, 473
RsaNI GTAC 2 cut(s) 178, 472
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 2 cut(s) 64, 589
Sau96I GGNCC 1 cut(s) 542
ScrFI CCNGG 1 cut(s) 732
SduI GDGCHC 1 cut(s) 124
SfaNI GCATC 2 cut(s) 140, 357
Sse9I AATT 1 cut(s) 321
SsiI CCGC 2 cut(s) 606, 753
StyD4I CCNGG 1 cut(s) 730
TaaI ACNGT 4 cut(s) 77, 481, 533, 585
TaiI ACGT 1 cut(s) 183
TasI AATT 1 cut(s) 321
TatI WGTACW 1 cut(s) 471
TscAI CASTG 2 cut(s) 259, 564
TseFI GTSAC 2 cut(s) 338, 579
TseI GCWGC 1 cut(s) 267
Tsp45I GTSAC 2 cut(s) 338, 579
TspDTI ATGAA 1 cut(s) 349
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 2 cut(s) 259, 564
Van91I CCANNNNNTGG 2 cut(s) 644, 683
XagI CCTNNNNNAGG 1 cut(s) 666
XapI RAATTY 1 cut(s) 321
XceI RCATGY 4 cut(s) 202, 408, 456, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.