Rroxscaffold_4G00299270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001223
Physical Location & Seq
Reverse (-)
19211442 .. 19213796
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299270.1

Sequence Viewer

Length: 846 bp
ATGGGGTTGCGCGACCTGGAGAAAATGAAGGTCCGCCAGGACTACCGGAATCTATGGCACAAAGATTTGATGAGCACCATGACTTTCGACCCCGCCTGTATATATGTTGTGGAGGATATATGCCATGTAGCGGAAAGTGTGGAGAGAAACACTGCCCTGCATTTTGTCTTTGCTGAAGTGGTGAACTCAGTGATATTGCTGAGTGCCTGGGCTGTATTTCTGATACAGTTTATTGCTCGTAAGGCCTTCATGTCACCATTTCCATACATTCAGTCTGGCGATGCGATCATGATAACTACGCAGCACAAGATTGAACTGGACAAAAGAGATGGCAAGTATGCTCCACAGCCAGTTATGGCAGCTCCTCCAACGCAGCAGATGTCACGCGCCGATCACTCAATTCCTTCCCCCGGATATCCACCTCAAGGATCTCCACCGATGGGATATCATCAACCTCCACCAATGGGATATCCTCATCAACCGTCAGGATATATGCATCCTCCGGCATATGGGCAGCCTATGAACGGGTCCTCAAAAGGCGGCCATGATCCATATCCTCCTGTTCCTTATCATCCACAACATCCACCATATCCACCTCAAATGTATCATCCACATGCTGCTTATTTTCCAGGGCCAGCACCAGGTTCTTATCCTGCAGGTCCACCACCAGGTCTTTATCCTCAAGGTCCAGACTCGGGTTTTGTTCCAGGTCCAGAATCTGGTCCTTATCCTCCTGCACGACCTCCTCCACCTCCTCCTGGCTATCACGCAGAACCAAGTGCACCTCTTCCTCCTCATGAAGAACACCCTGAAACTGCTCCGACTAGCAGCAATCCTCCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

30.83

Weight (kDa)

6.32

Isoelectric Point (pI)

80.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 647
AccB7I CCANNNNNTGG 1 cut(s) 719
AccII CGCG 2 cut(s) 12, 387
AciI CCGC 4 cut(s) 34, 93, 131, 540
AclWI GGATC 2 cut(s) 436, 542
AcoI YGGCCR 1 cut(s) 541
AcuI CTGAAG 1 cut(s) 195
AgsI TTSAA 1 cut(s) 314
AjnI CCWGG 8 cut(s) 15, 36, 206, 628, 640, 667, 706, 757
AluBI AGCT 1 cut(s) 362
AluI AGCT 1 cut(s) 362
Alw21I GWGCWC 2 cut(s) 77, 784
Alw44I GTGCAC 1 cut(s) 780
AlwI GGATC 2 cut(s) 436, 542
AlwNI CAGNNNCTG 1 cut(s) 719
Ama87I CYCGRG 1 cut(s) 694
AoxI GGCC 3 cut(s) 243, 541, 632
ApaLI GTGCAC 1 cut(s) 780
ApeKI GCWGC 6 cut(s) 301, 359, 373, 514, 617, 828
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
AspLEI GCGC 2 cut(s) 12, 389
AspS9I GGNCC 7 cut(s) 31, 528, 632, 659, 686, 710, 722
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 193, 246
AvaI CYCGRG 1 cut(s) 694
AvaII GGWCC 6 cut(s) 31, 528, 659, 686, 710, 722
BaeGI GKGCMC 1 cut(s) 784
Bbv12I GWGCWC 2 cut(s) 77, 784
BbvI GCAGC 6 cut(s) 313, 371, 385, 526, 604, 840
BccI CCATC 2 cut(s) 323, 433
BciT130I CCWGG 8 cut(s) 17, 38, 208, 630, 642, 669, 708, 759
BcnI CCSGG 1 cut(s) 411
BfaI CTAG 1 cut(s) 825
BfmI CTRYAG 1 cut(s) 654
BfuAI ACCTGC 1 cut(s) 647
BisI GCNGC 7 cut(s) 302, 360, 374, 515, 541, 618, 829
BlsI GCNGC 7 cut(s) 303, 361, 375, 516, 542, 619, 830
Bme1390I CCNGG 9 cut(s) 17, 38, 208, 411, 630, 642, 669, 708, 759
Bme18I GGWCC 6 cut(s) 31, 528, 659, 686, 710, 722
BmeT110I CYCGRG 1 cut(s) 694
BmgT120I GGNCC 7 cut(s) 31, 528, 632, 659, 686, 710, 722
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 9 cut(s) 17, 38, 208, 411, 630, 642, 669, 708, 759
BmsI GCATC 2 cut(s) 271, 505
BpmI CTGGAG 1 cut(s) 38
BpuEI CTTGAG 2 cut(s) 408, 666
BpuMI CCSGG 1 cut(s) 411
BsaBI GATNNNNATC 1 cut(s) 552
BsaJI CCNNGG 3 cut(s) 207, 409, 629
BsaWI WCCGGW 1 cut(s) 45
Bse1I ACTGG 2 cut(s) 321, 350
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 8 cut(s) 17, 38, 208, 630, 642, 669, 708, 759
BseDI CCNNGG 3 cut(s) 207, 409, 629
BseGI GGATG 4 cut(s) 496, 571, 580, 607
BseJI GATNNNNATC 1 cut(s) 552
BseMII CTCAG 2 cut(s) 191, 201
BseNI ACTGG 2 cut(s) 321, 350
BseRI GAGGAG 4 cut(s) 354, 735, 744, 783
BseSI GKGCMC 1 cut(s) 784
BseXI GCAGC 6 cut(s) 313, 371, 385, 526, 604, 840
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 2 cut(s) 12, 387
BshFI GGCC 3 cut(s) 245, 543, 634
BsiHKAI GWGCWC 2 cut(s) 77, 784
BsiHKCI CYCGRG 1 cut(s) 694
BsiSI CCGG 3 cut(s) 46, 411, 503
BsnI GGCC 3 cut(s) 245, 543, 634
BsoBI CYCGRG 1 cut(s) 694
Bsp1286I GDGCHC 2 cut(s) 77, 784
Bsp143I GATC 4 cut(s) 285, 391, 428, 547
BspACI CCGC 4 cut(s) 34, 93, 131, 540
BspANI GGCC 3 cut(s) 245, 543, 634
BspCNI CTCAG 2 cut(s) 192, 200
BspFNI CGCG 2 cut(s) 12, 387
BspHI TCATGA 2 cut(s) 288, 796
BspLI GGNNCC 1 cut(s) 529
BspMAI CTGCAG 1 cut(s) 658
BspMI ACCTGC 1 cut(s) 647
BspPI GGATC 2 cut(s) 436, 542
BsrI ACTGG 2 cut(s) 321, 350
BssECI CCNNGG 3 cut(s) 207, 409, 629
BssMI GATC 4 cut(s) 285, 391, 428, 547
Bst2UI CCWGG 8 cut(s) 17, 38, 208, 630, 642, 669, 708, 759
Bst4CI ACNGT 2 cut(s) 228, 483
Bst6I CTCTTC 1 cut(s) 792
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 2 cut(s) 187, 200
BstF5I GGATG 4 cut(s) 496, 571, 580, 607
BstFNI CGCG 2 cut(s) 12, 387
BstHHI GCGC 2 cut(s) 12, 389
BstKTI GATC 4 cut(s) 288, 394, 431, 550
BstMBI GATC 4 cut(s) 285, 391, 428, 547
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 8 cut(s) 17, 38, 208, 630, 642, 669, 708, 759
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 9 cut(s) 15, 36, 206, 409, 628, 640, 667, 706, 757
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 1 cut(s) 784
BstUI CGCG 2 cut(s) 12, 387
BstV1I GCAGC 6 cut(s) 313, 371, 385, 526, 604, 840
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 3 cut(s) 245, 543, 634
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 4 cut(s) 496, 571, 580, 607
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 150, 195
BveI ACCTGC 1 cut(s) 647
Cac8I GCNNGC 1 cut(s) 636
CaiI CAGNNNCTG 1 cut(s) 719
CciI TCATGA 2 cut(s) 288, 796
CfoI GCGC 2 cut(s) 12, 389
Cfr13I GGNCC 7 cut(s) 31, 528, 632, 659, 686, 710, 722
CsiI ACCWGGT 2 cut(s) 640, 667
CviAII CATG 7 cut(s) 79, 125, 250, 289, 545, 614, 797
CviJI RGCY 8 cut(s) 212, 245, 349, 362, 517, 543, 634, 762
CviKI_1 RGCY 8 cut(s) 212, 245, 349, 362, 517, 543, 634, 762
DdeI CTNAG 2 cut(s) 187, 200
DpnI GATC 4 cut(s) 287, 393, 430, 549
DpnII GATC 4 cut(s) 285, 391, 428, 547
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
EciI GGCGGA 1 cut(s) 23
Eco147I AGGCCT 1 cut(s) 245
Eco32I GATATC 3 cut(s) 416, 446, 470
Eco47I GGWCC 6 cut(s) 31, 528, 659, 686, 710, 722
Eco57I CTGAAG 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 694
EcoO109I RGGNCCY 1 cut(s) 528
EcoRII CCWGG 8 cut(s) 15, 36, 206, 628, 640, 667, 706, 757
EcoRV GATATC 3 cut(s) 416, 446, 470
EcoT22I ATGCAT 1 cut(s) 498
FaeI CATG 7 cut(s) 82, 128, 253, 292, 548, 617, 800
FatI CATG 7 cut(s) 78, 124, 249, 288, 544, 613, 796
FauI CCCGC 1 cut(s) 100
FauNDI CATATG 1 cut(s) 508
Fnu4HI GCNGC 7 cut(s) 302, 360, 374, 515, 541, 618, 829
FokI GGATG 4 cut(s) 483, 558, 567, 594
Fsp4HI GCNGC 7 cut(s) 302, 360, 374, 515, 541, 618, 829
FspBI CTAG 1 cut(s) 825
GlaI GCGC 2 cut(s) 11, 388
GluI GCNGC 7 cut(s) 302, 360, 374, 515, 541, 618, 829
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 3 cut(s) 245, 543, 634
HapII CCGG 3 cut(s) 46, 411, 503
HhaI GCGC 2 cut(s) 12, 389
Hin1II CATG 7 cut(s) 82, 128, 253, 292, 548, 617, 800
Hin6I GCGC 2 cut(s) 10, 387
HinP1I GCGC 2 cut(s) 10, 387
HinfI GANTC 3 cut(s) 49, 692, 716
HpaII CCGG 3 cut(s) 46, 411, 503
HphI GGTGA 2 cut(s) 193, 246
Hpy166II GTNNAC 3 cut(s) 184, 662, 782
Hpy188I TCNGA 2 cut(s) 222, 822
Hpy188III TCNNGA 5 cut(s) 289, 486, 689, 713, 797
Hpy8I GTNNAC 3 cut(s) 184, 662, 782
HpyAV CCTTC 3 cut(s) 22, 256, 414
HpyCH4III ACNGT 2 cut(s) 228, 483
HpyCH4V TGCA 5 cut(s) 160, 496, 656, 737, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 2 cut(s) 187, 200
Hsp92II CATG 7 cut(s) 82, 128, 253, 292, 548, 617, 800
HspAI GCGC 2 cut(s) 10, 387
Kzo9I GATC 4 cut(s) 285, 391, 428, 547
LmnI GCTCC 3 cut(s) 346, 367, 823
Lsp1109I GCAGC 6 cut(s) 313, 371, 385, 526, 604, 840
LweI GCATC 2 cut(s) 271, 505
MabI ACCWGGT 2 cut(s) 640, 667
MaeI CTAG 1 cut(s) 825
MaeIII GTNAC 2 cut(s) 252, 381
MalI GATC 4 cut(s) 287, 393, 430, 549
MboI GATC 4 cut(s) 285, 391, 428, 547
MboII GAAGA 2 cut(s) 779, 812
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 2 cut(s) 77, 784
MluCI AATT 1 cut(s) 399
MlyI GAGTC 1 cut(s) 686
MmeI TCCRAC 2 cut(s) 392, 845
Mph1103I ATGCAT 1 cut(s) 498
MslI CAYNNNNRTG 1 cut(s) 612
MspI CCGG 3 cut(s) 46, 411, 503
MspR9I CCNGG 9 cut(s) 17, 38, 208, 411, 630, 642, 669, 708, 759
MvaI CCWGG 8 cut(s) 17, 38, 208, 630, 642, 669, 708, 759
MvnI CGCG 2 cut(s) 12, 387
MwoI GCNNNNNNNGC 1 cut(s) 242
NciI CCSGG 1 cut(s) 411
NdeI CATATG 1 cut(s) 508
NdeII GATC 4 cut(s) 285, 391, 428, 547
NlaIII CATG 7 cut(s) 82, 128, 253, 292, 548, 617, 800
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 2 cut(s) 252, 381
NsiI ATGCAT 1 cut(s) 498
NspI RCATGY 1 cut(s) 617
PagI TCATGA 2 cut(s) 288, 796
PceI AGGCCT 1 cut(s) 245
PfeI GAWTC 2 cut(s) 49, 716
PflMI CCANNNNNTGG 1 cut(s) 719
PkrI GCNGC 7 cut(s) 303, 361, 375, 516, 542, 619, 830
PleI GAGTC 1 cut(s) 686
PpsI GAGTC 1 cut(s) 686
PpuMI RGGWCCY 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 528
Psp6I CCWGG 8 cut(s) 15, 36, 206, 628, 640, 667, 706, 757
PspGI CCWGG 8 cut(s) 15, 36, 206, 628, 640, 667, 706, 757
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 7 cut(s) 31, 528, 632, 659, 686, 710, 722
PspPPI RGGWCCY 1 cut(s) 528
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 719
PsuI RGATCY 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 612
SatI GCNGC 7 cut(s) 302, 360, 374, 515, 541, 618, 829
Sau3AI GATC 4 cut(s) 285, 391, 428, 547
Sau96I GGNCC 7 cut(s) 31, 528, 632, 659, 686, 710, 722
SbfI CCTGCAGG 1 cut(s) 658
SchI GAGTC 1 cut(s) 686
ScrFI CCNGG 9 cut(s) 17, 38, 208, 411, 630, 642, 669, 708, 759
SdaI CCTGCAGG 1 cut(s) 658
SduI GDGCHC 2 cut(s) 77, 784
SexAI ACCWGGT 2 cut(s) 640, 667
SfaNI GCATC 2 cut(s) 271, 505
SfcI CTRYAG 1 cut(s) 654
SinI GGWCC 6 cut(s) 31, 528, 659, 686, 710, 722
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 2 cut(s) 423, 681
SmoI CTYRAG 2 cut(s) 423, 681
Sse8387I CCTGCAGG 1 cut(s) 658
Sse9I AATT 1 cut(s) 399
SseBI AGGCCT 1 cut(s) 245
SsiI CCGC 4 cut(s) 34, 93, 131, 540
SspMI CTAG 1 cut(s) 825
StuI AGGCCT 1 cut(s) 245
StyD4I CCNGG 9 cut(s) 15, 36, 206, 409, 628, 640, 667, 706, 757
TaaI ACNGT 2 cut(s) 228, 483
TaqI TCGA 1 cut(s) 87
TasI AATT 1 cut(s) 399
TauI GCSGC 1 cut(s) 543
TfiI GAWTC 2 cut(s) 49, 716
TscAI CASTG 2 cut(s) 157, 195
TseFI GTSAC 2 cut(s) 252, 381
TseI GCWGC 6 cut(s) 301, 359, 373, 514, 617, 828
Tsp45I GTSAC 2 cut(s) 252, 381
TspDTI ATGAA 4 cut(s) 41, 238, 536, 813
TspRI CASTG 2 cut(s) 157, 195
Van91I CCANNNNNTGG 1 cut(s) 719
VneI GTGCAC 1 cut(s) 780
VpaK11BI GGWCC 6 cut(s) 31, 528, 659, 686, 710, 722
XceI RCATGY 1 cut(s) 617
XspI CTAG 1 cut(s) 825
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.