pycom07g13100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001223
Physical Location & Seq
Reverse (-)
14679395 .. 14680625
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g13100.1

Sequence Viewer

Length: 831 bp
ATGAACTTTTACTTTATATATGTTTGCATACTTTGTTGTTTTCAGGTCTGCTGCTGCTTTGCAAGTTCAGTTTCCTCGACACGCTTTCTAATCCAAGATGAGTTCAATATTCAAACAACACCGTGCGACAATTGCATTATTGGATGTATGCTCTGCCTCAACCAACTTGCATGTATATGCTCCTTGATTGCTTGCTTAACTGGAAGCGAAGAGCTCTCAGATATTTCCAGTGTGTTGAACTGTATAGCTGACATGGTGTTCTGCTCGGTCTGTGCTTGCATGCAGACCCAGCACAAGATTGAACTGGACAAAAGGGATGGCAAGCTTGATCAACCACGCGTGATGGCAGTTCCTCCGATGCAGCAGATGTCACGGATTGATCAGGCAGTTTCTCCTCATGCCGGATATAAACATACACACGCTGGATATCCACTTCCACCAACAGGATATCCAAATCAACCGGTAGGATATCCAACTCCACCGGCAGGATATCCAAATCAACCGCCAGGATATCCACCCGCGGGATATCCGAACCAACATCCAGGGTATCCAAACCAAAATGCAGGGTATGGACATCATCAGCCAGTTGGATATATGCATCCACCGGCATATGGGCAACCAGTATATCCTCCAGGGCCTCCACCTTCTGCGCATCCTCCTCCAGAGTCGGGAGAATTTTATAACCCCGGACCTCCTCCTCCACCTCCTGGCTATACTCCAGAATCTGCATCTGCTCCTCCATATCCTCCAGGTCCTCCACCGATTTCTCATCAGGAGGCTTATCCTCAACCTTCTCCTCTTAGCCGCACCGGTACCGATCCTCCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

277

Amino Acids

29.81

Weight (kDa)

5.72

Isoelectric Point (pI)

64.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 681
Acc16I TGCGCA 1 cut(s) 651
Acc65I GGTACC 1 cut(s) 812
AccB1I GGYRCC 1 cut(s) 812
AccB7I CCANNNNNTGG 1 cut(s) 707
AccII CGCG 2 cut(s) 339, 521
AciI CCGC 4 cut(s) 503, 519, 521, 805
AclWI GGATC 1 cut(s) 812
AcsI RAATTY 1 cut(s) 674
AfaI GTAC 1 cut(s) 814
AfiI CCNNNNNNNGG 7 cut(s) 401, 443, 485, 521, 611, 668, 707
AflIII ACRYGT 1 cut(s) 337
AgeI ACCGGT 2 cut(s) 460, 809
AgsI TTSAA 4 cut(s) 106, 113, 238, 302
AjnI CCWGG 5 cut(s) 505, 541, 631, 706, 748
AluBI AGCT 3 cut(s) 214, 248, 325
AluI AGCT 3 cut(s) 214, 248, 325
Alw21I GWGCWC 1 cut(s) 216
AlwI GGATC 1 cut(s) 812
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 1 cut(s) 635
ApeKI GCWGC 3 cut(s) 51, 54, 361
ApoI RAATTY 1 cut(s) 674
AsiGI ACCGGT 2 cut(s) 460, 809
Asp718I GGTACC 1 cut(s) 812
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 3 cut(s) 635, 689, 752
AsuC2I CCSGG 1 cut(s) 687
AvaII GGWCC 2 cut(s) 689, 752
BanI GGYRCC 1 cut(s) 812
BanII GRGCYC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 3 cut(s) 38, 41, 373
BccI CCATC 2 cut(s) 311, 337
BciT130I CCWGG 5 cut(s) 507, 543, 633, 708, 750
BciVI GTATCC 1 cut(s) 558
BclI TGATCA 2 cut(s) 328, 379
BcnI CCSGG 1 cut(s) 687
BfuI GTATCC 1 cut(s) 558
BisI GCNGC 4 cut(s) 52, 55, 362, 805
BlsI GCNGC 4 cut(s) 53, 56, 363, 806
Bme1390I CCNGG 6 cut(s) 507, 543, 633, 687, 708, 750
Bme18I GGWCC 2 cut(s) 689, 752
BmgT120I GGNCC 3 cut(s) 635, 689, 752
BmiI GGNNCC 1 cut(s) 814
BmrFI CCNGG 6 cut(s) 507, 543, 633, 687, 708, 750
BmsI GCATC 4 cut(s) 348, 607, 661, 737
BpmI CTGGAG 4 cut(s) 615, 645, 702, 732
BpuMI CCSGG 1 cut(s) 687
BsaJI CCNNGG 4 cut(s) 519, 542, 632, 685
BsaWI WCCGGW 2 cut(s) 460, 809
BsaXI ACNNNNNCTCC 1 cut(s) 805
Bsc4I CCNNNNNNNGG 7 cut(s) 401, 443, 485, 521, 611, 668, 707
Bse118I RCCGGY 4 cut(s) 460, 481, 604, 809
Bse1I ACTGG 5 cut(s) 205, 228, 309, 584, 620
BseBI CCWGG 5 cut(s) 507, 543, 633, 708, 750
BseDI CCNNGG 4 cut(s) 519, 542, 632, 685
BseGI GGATG 5 cut(s) 149, 322, 538, 598, 652
BseLI CCNNNNNNNGG 7 cut(s) 401, 443, 485, 521, 611, 668, 707
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 5 cut(s) 205, 228, 309, 584, 620
BseRI GAGGAG 6 cut(s) 384, 648, 684, 687, 726, 786
BseXI GCAGC 3 cut(s) 38, 41, 373
BseYI CCCAGC 1 cut(s) 288
Bsh1236I CGCG 2 cut(s) 339, 521
BshFI GGCC 1 cut(s) 637
BshNI GGYRCC 1 cut(s) 812
BshTI ACCGGT 2 cut(s) 460, 809
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 6 cut(s) 402, 461, 482, 605, 687, 810
BslI CCNNNNNNNGG 7 cut(s) 401, 443, 485, 521, 611, 668, 707
BsnI GGCC 1 cut(s) 637
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 3 cut(s) 328, 379, 817
BspACI CCGC 4 cut(s) 503, 519, 521, 805
BspANI GGCC 1 cut(s) 637
BspCNI CTCAG 1 cut(s) 230
BspFNI CGCG 2 cut(s) 339, 521
BspLI GGNNCC 1 cut(s) 814
BspPI GGATC 1 cut(s) 812
BspQI GCTCTTC 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 812
BsrFI RCCGGY 4 cut(s) 460, 481, 604, 809
BsrI ACTGG 5 cut(s) 205, 228, 309, 584, 620
BssAI RCCGGY 4 cut(s) 460, 481, 604, 809
BssECI CCNNGG 4 cut(s) 519, 542, 632, 685
BssMI GATC 3 cut(s) 328, 379, 817
Bst2UI CCWGG 5 cut(s) 507, 543, 633, 708, 750
Bst4CI ACNGT 2 cut(s) 123, 242
Bst6I CTCTTC 1 cut(s) 204
BstC8I GCNNGC 4 cut(s) 193, 277, 281, 323
BstDEI CTNAG 2 cut(s) 217, 800
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 5 cut(s) 149, 322, 538, 598, 652
BstFNI CGCG 2 cut(s) 339, 521
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 3 cut(s) 331, 382, 820
BstMBI GATC 3 cut(s) 328, 379, 817
BstMWI GCNNNNNNNGC 2 cut(s) 132, 289
BstNI CCWGG 5 cut(s) 507, 543, 633, 708, 750
BstNSI RCATGY 2 cut(s) 174, 283
BstSCI CCNGG 6 cut(s) 505, 541, 631, 685, 706, 748
BstUI CGCG 2 cut(s) 339, 521
BstV1I GCAGC 3 cut(s) 38, 41, 373
BsuI GTATCC 1 cut(s) 558
BsuRI GGCC 1 cut(s) 637
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 5 cut(s) 149, 322, 538, 598, 652
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 4 cut(s) 193, 277, 281, 323
CaiI CAGNNNCTG 1 cut(s) 725
CfoI GCGC 1 cut(s) 652
Cfr10I RCCGGY 4 cut(s) 460, 481, 604, 809
Cfr13I GGNCC 3 cut(s) 635, 689, 752
Cfr42I CCGCGG 1 cut(s) 522
Csp6I GTAC 1 cut(s) 813
CspAI ACCGGT 2 cut(s) 460, 809
CviAII CATG 4 cut(s) 171, 253, 280, 398
CviJI RGCY 8 cut(s) 214, 248, 325, 583, 637, 711, 779, 804
CviKI_1 RGCY 8 cut(s) 214, 248, 325, 583, 637, 711, 779, 804
CviQI GTAC 1 cut(s) 813
DdeI CTNAG 2 cut(s) 217, 800
DpnI GATC 3 cut(s) 330, 381, 819
DpnII GATC 3 cut(s) 328, 379, 817
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 216
Eco32I GATATC 6 cut(s) 428, 449, 470, 491, 512, 527
Eco47I GGWCC 2 cut(s) 689, 752
Eco53kI GAGCTC 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 214
EcoO109I RGGNCCY 2 cut(s) 635, 752
EcoRII CCWGG 5 cut(s) 505, 541, 631, 706, 748
EcoRV GATATC 6 cut(s) 428, 449, 470, 491, 512, 527
EcoT22I ATGCAT 1 cut(s) 600
EcoT38I GRGCYC 1 cut(s) 216
FaeI CATG 4 cut(s) 174, 256, 283, 401
FatI CATG 4 cut(s) 170, 252, 279, 397
FauI CCCGC 2 cut(s) 514, 526
FauNDI CATATG 1 cut(s) 610
FbaI TGATCA 2 cut(s) 328, 379
Fnu4HI GCNGC 4 cut(s) 52, 55, 362, 805
FokI GGATG 5 cut(s) 156, 329, 525, 585, 639
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 4 cut(s) 52, 55, 362, 805
FspI TGCGCA 1 cut(s) 651
GlaI GCGC 1 cut(s) 651
GluI GCNGC 4 cut(s) 52, 55, 362, 805
GsaI CCCAGC 1 cut(s) 292
GsuI CTGGAG 4 cut(s) 615, 645, 702, 732
HaeIII GGCC 1 cut(s) 637
HapII CCGG 6 cut(s) 402, 461, 482, 605, 687, 810
HhaI GCGC 1 cut(s) 652
Hin1II CATG 4 cut(s) 174, 256, 283, 401
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 2 cut(s) 665, 722
HpaII CCGG 6 cut(s) 402, 461, 482, 605, 687, 810
Hpy188I TCNGA 3 cut(s) 220, 357, 531
Hpy188III TCNNGA 4 cut(s) 662, 669, 719, 773
HpyAV CCTTC 2 cut(s) 654, 801
HpyCH4III ACNGT 2 cut(s) 123, 242
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 289
HpyF3I CTNAG 2 cut(s) 217, 800
Hsp92II CATG 4 cut(s) 174, 256, 283, 401
HspAI GCGC 1 cut(s) 650
KpnI GGTACC 1 cut(s) 816
Ksp22I TGATCA 2 cut(s) 328, 379
KspI CCGCGG 1 cut(s) 522
Kzo9I GATC 3 cut(s) 328, 379, 817
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 2 cut(s) 185, 739
Lsp1109I GCAGC 3 cut(s) 38, 41, 373
LweI GCATC 4 cut(s) 348, 607, 661, 737
MaeIII GTNAC 1 cut(s) 369
MalI GATC 3 cut(s) 330, 381, 819
MboI GATC 3 cut(s) 328, 379, 817
MboII GAAGA 1 cut(s) 221
MfeI CAATTG 1 cut(s) 130
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 2 cut(s) 130, 674
MluI ACGCGT 1 cut(s) 337
MlyI GAGTC 1 cut(s) 674
MmeI TCCRAC 2 cut(s) 497, 568
Mph1103I ATGCAT 1 cut(s) 600
MseI TTAA 1 cut(s) 197
MslI CAYNNNNRTG 1 cut(s) 175
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 6 cut(s) 402, 461, 482, 605, 687, 810
MspR9I CCNGG 6 cut(s) 507, 543, 633, 687, 708, 750
MunI CAATTG 1 cut(s) 130
MvaI CCWGG 5 cut(s) 507, 543, 633, 708, 750
MvnI CGCG 2 cut(s) 339, 521
MwoI GCNNNNNNNGC 2 cut(s) 132, 289
NciI CCSGG 1 cut(s) 687
NdeI CATATG 1 cut(s) 610
NdeII GATC 3 cut(s) 328, 379, 817
NlaIII CATG 4 cut(s) 174, 256, 283, 401
NlaIV GGNNCC 1 cut(s) 814
NmuCI GTSAC 1 cut(s) 369
NsbI TGCGCA 1 cut(s) 651
NsiI ATGCAT 1 cut(s) 600
NspI RCATGY 2 cut(s) 174, 283
PaeI GCATGC 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 204
PfeI GAWTC 1 cut(s) 722
PflMI CCANNNNNTGG 1 cut(s) 707
PinAI ACCGGT 2 cut(s) 460, 809
PkrI GCNGC 4 cut(s) 53, 56, 363, 806
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
PpuMI RGGWCCY 1 cut(s) 752
PsiI TTATAA 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 5 cut(s) 505, 541, 631, 706, 748
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 5 cut(s) 505, 541, 631, 706, 748
PspN4I GGNNCC 1 cut(s) 814
PspPI GGNCC 3 cut(s) 635, 689, 752
PspPPI RGGWCCY 1 cut(s) 752
PstNI CAGNNNCTG 1 cut(s) 725
RsaI GTAC 1 cut(s) 814
RsaNI GTAC 1 cut(s) 813
RseI CAYNNNNRTG 1 cut(s) 175
SacI GAGCTC 1 cut(s) 216
SacII CCGCGG 1 cut(s) 522
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 4 cut(s) 52, 55, 362, 805
Sau3AI GATC 3 cut(s) 328, 379, 817
Sau96I GGNCC 3 cut(s) 635, 689, 752
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 6 cut(s) 507, 543, 633, 687, 708, 750
SduI GDGCHC 1 cut(s) 216
SetI ASST 9 cut(s) 48, 216, 250, 327, 646, 694, 706, 754, 793
SfaNI GCATC 4 cut(s) 348, 607, 661, 737
Sfr303I CCGCGG 1 cut(s) 522
SgrBI CCGCGG 1 cut(s) 522
SinI GGWCC 2 cut(s) 689, 752
SmiMI CAYNNNNRTG 1 cut(s) 175
SphI GCATGC 1 cut(s) 283
Sse9I AATT 2 cut(s) 130, 674
SsiI CCGC 4 cut(s) 503, 519, 521, 805
SspI AATATT 1 cut(s) 109
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 6 cut(s) 505, 541, 631, 685, 706, 748
TaaI ACNGT 2 cut(s) 123, 242
TaqI TCGA 1 cut(s) 77
TaqII GACCGA 1 cut(s) 256
TasI AATT 2 cut(s) 130, 674
TauI GCSGC 1 cut(s) 807
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 369
TseI GCWGC 3 cut(s) 51, 54, 361
Tsp45I GTSAC 1 cut(s) 369
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 1 cut(s) 235
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 2 cut(s) 689, 752
XapI RAATTY 1 cut(s) 674
XceI RCATGY 2 cut(s) 174, 283
Zsp2I ATGCAT 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.