RH1DG262500

No description available

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0003426
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG262500.1

Sequence Viewer

Length: 882 bp
ATGGTCAGGATCCGCAAGAACTTGAAGGTGTCGCCGCTCCAGTTCTTCCCCAACGTAGCTCCTGAGGACCTGCAGCCCCACATCTGCAAGCTGAACCAGTCTCCTTGGGATTCGAGCCCCTGCCCTTTCGACCTCCTCCTCGACTCGTCGCCGCTCCAGTTCGAAGCTGGGGAGGGTTTTATCGGAAATGGGAGCTTGGGGGATTCTATTACAGCTGTCGAGAGCGTTGCGTCGCCGATGGATATCGACAAGGCAGAAATGGCGACCGGCGATGTGTTGGATGACGTCGACAGAGCAATTGTGAGGTGTCAAAAGAACGACGGCAGGAGCTGGCAATGCAAGAAGGAAGCGAAAGAAGGCTTCTTATTTTGTGACCATCATATCCGAAGGTCGTCCTATGGTAACAACAACGTTACTAGCAATGGCAATATTCTTCCCTCCAACTTCGTGGCTTCGAAGAAGGACACGGCGGCGGCCCCGGCCAAGACGCTAGCCGGAACCCGCCGTGCCAGACCCAAGGCGGTGAAGAAGGCGGCTTCGTCGGCGAACCCGTATGAGTTCTACTATTATTCGGGGTTCGGCCCAAAATGGGGCCGAAAGAGAGGCAAGAAAGGCGAGGTGGTGGAGGAGGAGGAGGAGGAGGCCAATAATATTGAGTCAGCAGCTGGAAAGGTTGCAACTTCTTGTAGTACTAGTAGTGCTAACACCGATCCCCAACCTTCTTCACCTCACAACGTTGGTAATGGGGATCAATTGGACTACATGGAAGACGACTTCGACGACGAGGATGGGAGCGACGAAGAAGGCGGCCCGAGGAGGATGAGGAGGCCGGTGAAGGCTCGATCCTTGATGTCCATAATTAATGTAAAAGCTCAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

293

Amino Acids

32.05

Weight (kDa)

5.45

Isoelectric Point (pI)

68.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 101 - 134 3.2e-09 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 78
AccBSI CCGCTC 2 cut(s) 37, 154
AccI GTMKAC 1 cut(s) 288
AciI CCGC 9 cut(s) 13, 35, 152, 470, 473, 502, 521, 533, 807
AclI AACGTT 2 cut(s) 411, 735
AclWI GGATC 5 cut(s) 4, 17, 704, 756, 837
AcoI YGGCCR 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 1 cut(s) 691
AfiI CCNNNNNNNGG 2 cut(s) 589, 590
AgsI TTSAA 1 cut(s) 25
AhlI ACTAGT 1 cut(s) 692
AluBI AGCT 8 cut(s) 59, 91, 167, 195, 215, 330, 665, 872
AluI AGCT 8 cut(s) 59, 91, 167, 195, 215, 330, 665, 872
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 5 cut(s) 4, 17, 704, 756, 837
AlwNI CAGNNNCTG 2 cut(s) 330, 665
Ama87I CYCGRG 1 cut(s) 811
AoxI GGCC 7 cut(s) 474, 480, 580, 592, 642, 808, 827
ApeKI GCWGC 2 cut(s) 73, 662
AseI ATTAAT 1 cut(s) 861
AspS9I GGNCC 5 cut(s) 67, 475, 581, 592, 809
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 3 cut(s) 535, 717, 844
AsuII TTCGAA 2 cut(s) 162, 455
AsuNHI GCTAGC 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 811
AvaII GGWCC 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 63
BamHI GGATCC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 774
BbvI GCAGC 2 cut(s) 85, 674
BccI CCATC 3 cut(s) 232, 384, 782
BceAI ACGGC 3 cut(s) 337, 483, 489
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 692
BfaI CTAG 3 cut(s) 417, 491, 693
BfmI CTRYAG 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 78
BisI GCNGC 8 cut(s) 35, 74, 152, 471, 474, 534, 663, 808
BlsI GCNGC 8 cut(s) 36, 75, 153, 472, 475, 535, 664, 809
BmcAI AGTACT 1 cut(s) 691
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 811
BmgT120I GGNCC 5 cut(s) 67, 475, 581, 592, 809
BmiI GGNNCC 4 cut(s) 11, 477, 499, 593
BmrFI CCNGG 1 cut(s) 479
BmtI GCTAGC 1 cut(s) 494
BpiI GAAGAC 1 cut(s) 774
BpmI CTGGAG 2 cut(s) 23, 140
Bpu14I TTCGAA 2 cut(s) 162, 455
BpuEI CTTGAG 1 cut(s) 858
BpuMI CCSGG 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 4 cut(s) 104, 477, 516, 812
Bsc4I CCNNNNNNNGG 2 cut(s) 589, 590
Bse118I RCCGGY 2 cut(s) 266, 829
Bse1I ACTGG 3 cut(s) 40, 97, 157
Bse21I CCTNAGG 1 cut(s) 63
Bse3DI GCAATG 2 cut(s) 341, 427
Bse8I GATNNNNATC 1 cut(s) 242
BseDI CCNNGG 4 cut(s) 104, 477, 516, 812
BseGI GGATG 3 cut(s) 286, 793, 825
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 589, 590
BseMI GCAATG 2 cut(s) 341, 427
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 3 cut(s) 40, 97, 157
BseRI GAGGAG 9 cut(s) 125, 128, 641, 644, 647, 650, 653, 829, 838
BseXI GCAGC 2 cut(s) 85, 674
BseYI CCCAGC 1 cut(s) 167
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 7 cut(s) 476, 482, 582, 594, 644, 810, 829
BsiEI CGRYCG 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 811
BsiSI CCGG 4 cut(s) 267, 479, 495, 830
BslI CCNNNNNNNGG 2 cut(s) 589, 590
BsmAI GTCTC 1 cut(s) 105
BsnI GGCC 7 cut(s) 476, 482, 582, 594, 644, 810, 829
BsoBI CYCGRG 1 cut(s) 811
Bsp119I TTCGAA 2 cut(s) 162, 455
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 4 cut(s) 9, 709, 748, 842
BspACI CCGC 9 cut(s) 13, 35, 152, 470, 473, 502, 521, 533, 807
BspANI GGCC 7 cut(s) 476, 482, 582, 594, 644, 810, 829
BspCNI CTCAG 1 cut(s) 55
BspLI GGNNCC 4 cut(s) 11, 477, 499, 593
BspMAI CTGCAG 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 78
BspOI GCTAGC 1 cut(s) 494
BspPI GGATC 5 cut(s) 4, 17, 704, 756, 837
BspT104I TTCGAA 2 cut(s) 162, 455
BsrBI CCGCTC 2 cut(s) 37, 154
BsrDI GCAATG 2 cut(s) 341, 427
BsrFI RCCGGY 2 cut(s) 266, 829
BsrI ACTGG 3 cut(s) 40, 97, 157
BssAI RCCGGY 2 cut(s) 266, 829
BssECI CCNNGG 4 cut(s) 104, 477, 516, 812
BssMI GATC 4 cut(s) 9, 709, 748, 842
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 2 cut(s) 104, 516
BstACI GRCGYC 1 cut(s) 285
BstBI TTCGAA 2 cut(s) 162, 455
BstC8I GCNNGC 3 cut(s) 89, 332, 492
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 3 cut(s) 286, 793, 825
BstKTI GATC 4 cut(s) 12, 712, 751, 845
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 4 cut(s) 9, 709, 748, 842
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 5 cut(s) 260, 336, 479, 542, 612
BstSCI CCNGG 1 cut(s) 477
BstSFI CTRYAG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 85, 674
BstV2I GAAGAC 1 cut(s) 774
BstX2I RGATCY 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 448
BstYI RGATCY 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 63
BsuRI GGCC 7 cut(s) 476, 482, 582, 594, 644, 810, 829
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 3 cut(s) 286, 793, 825
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 3 cut(s) 89, 332, 492
CaiI CAGNNNCTG 2 cut(s) 330, 665
Cfr10I RCCGGY 2 cut(s) 266, 829
Cfr13I GGNCC 5 cut(s) 67, 475, 581, 592, 809
CseI GACGC 2 cut(s) 219, 496
Csp6I GTAC 1 cut(s) 690
CviAII CATG 1 cut(s) 763
CviQI GTAC 1 cut(s) 690
DdeI CTNAG 1 cut(s) 63
DpnI GATC 4 cut(s) 11, 711, 750, 844
DpnII GATC 4 cut(s) 9, 709, 748, 842
EaeI YGGCCR 1 cut(s) 480
Eco130I CCWWGG 2 cut(s) 104, 516
Eco24I GRGCYC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 67
Eco81I CCTNAGG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 811
EcoO109I RGGNCCY 1 cut(s) 67
EcoRV GATATC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 104, 516
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 2 cut(s) 104, 516
FaeI CATG 1 cut(s) 766
FaiI YATR 5 cut(s) 381, 399, 555, 764, 857
FatI CATG 1 cut(s) 762
FauI CCCGC 1 cut(s) 509
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 8 cut(s) 35, 74, 152, 471, 474, 534, 663, 808
FokI GGATG 3 cut(s) 293, 800, 832
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 8 cut(s) 35, 74, 152, 471, 474, 534, 663, 808
FspBI CTAG 3 cut(s) 417, 491, 693
GluI GCNGC 8 cut(s) 35, 74, 152, 471, 474, 534, 663, 808
GsaI CCCAGC 1 cut(s) 171
GsuI CTGGAG 2 cut(s) 23, 140
HaeIII GGCC 7 cut(s) 476, 482, 582, 594, 644, 810, 829
HapII CCGG 4 cut(s) 267, 479, 495, 830
HgaI GACGC 2 cut(s) 219, 496
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 1 cut(s) 766
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 4 cut(s) 110, 143, 203, 656
HpaII CCGG 4 cut(s) 267, 479, 495, 830
HphI GGTGA 3 cut(s) 535, 717, 844
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 2 cut(s) 185, 386
Hpy188III TCNNGA 4 cut(s) 7, 62, 220, 875
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 8 cut(s) 151, 235, 290, 323, 544, 782, 785, 800
HpyAV CCTTC 9 cut(s) 19, 337, 350, 381, 454, 523, 729, 797, 829
HpyCH4IV ACGT 4 cut(s) 54, 285, 411, 735
HpyCH4V TGCA 4 cut(s) 73, 87, 339, 677
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 336, 479, 542, 612
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 4 cut(s) 54, 285, 411, 735
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 1 cut(s) 766
Kzo9I GATC 4 cut(s) 9, 709, 748, 842
LmnI GCTCC 6 cut(s) 42, 64, 159, 192, 327, 792
Lsp1109I GCAGC 2 cut(s) 85, 674
MaeI CTAG 3 cut(s) 417, 491, 693
MaeII ACGT 4 cut(s) 54, 285, 411, 735
MaeIII GTNAC 3 cut(s) 371, 401, 412
MalI GATC 4 cut(s) 11, 711, 750, 844
MbiI CCGCTC 2 cut(s) 37, 154
MboI GATC 4 cut(s) 9, 709, 748, 842
MboII GAAGA 7 cut(s) 37, 425, 469, 538, 714, 779, 812
MfeI CAATTG 2 cut(s) 297, 752
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 3 cut(s) 297, 752, 858
MlyI GAGTC 2 cut(s) 137, 665
MmeI TCCRAC 2 cut(s) 258, 465
MseI TTAA 1 cut(s) 861
MspA1I CMGCKG 2 cut(s) 215, 665
MspI CCGG 4 cut(s) 267, 479, 495, 830
MspR9I CCNGG 1 cut(s) 479
MunI CAATTG 2 cut(s) 297, 752
MwoI GCNNNNNNNGC 5 cut(s) 260, 336, 479, 542, 612
NciI CCSGG 1 cut(s) 479
NdeII GATC 4 cut(s) 9, 709, 748, 842
NheI GCTAGC 1 cut(s) 490
NlaIII CATG 1 cut(s) 766
NlaIV GGNNCC 4 cut(s) 11, 477, 499, 593
NmuCI GTSAC 1 cut(s) 371
NspV TTCGAA 2 cut(s) 162, 455
PcsI WCGNNNNNNNCGW 2 cut(s) 548, 777
PfeI GAWTC 2 cut(s) 110, 203
PkrI GCNGC 8 cut(s) 36, 75, 153, 472, 475, 535, 664, 809
PleI GAGTC 2 cut(s) 137, 664
PpsI GAGTC 2 cut(s) 137, 664
PpuMI RGGWCCY 1 cut(s) 67
PshBI ATTAAT 1 cut(s) 861
Psp1406I AACGTT 2 cut(s) 411, 735
Psp5II RGGWCCY 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 167
PspN4I GGNNCC 4 cut(s) 11, 477, 499, 593
PspPI GGNCC 5 cut(s) 67, 475, 581, 592, 809
PspPPI RGGWCCY 1 cut(s) 67
PstI CTGCAG 1 cut(s) 75
PstNI CAGNNNCTG 2 cut(s) 330, 665
PsuI RGATCY 1 cut(s) 9
PvuII CAGCTG 2 cut(s) 215, 665
RsaI GTAC 1 cut(s) 691
RsaNI GTAC 1 cut(s) 690
SalI GTCGAC 1 cut(s) 287
SaqAI TTAA 1 cut(s) 861
SatI GCNGC 8 cut(s) 35, 74, 152, 471, 474, 534, 663, 808
Sau3AI GATC 4 cut(s) 9, 709, 748, 842
Sau96I GGNCC 5 cut(s) 67, 475, 581, 592, 809
ScaI AGTACT 1 cut(s) 691
SchI GAGTC 2 cut(s) 137, 665
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 71
SfuI TTCGAA 2 cut(s) 162, 455
SinI GGWCC 1 cut(s) 67
SmlI CTYRAG 1 cut(s) 873
SmoI CTYRAG 1 cut(s) 873
SpeI ACTAGT 1 cut(s) 692
Sse9I AATT 3 cut(s) 297, 752, 858
SsiI CCGC 9 cut(s) 13, 35, 152, 470, 473, 502, 521, 533, 807
SspI AATATT 2 cut(s) 430, 652
SspMI CTAG 3 cut(s) 417, 491, 693
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 2 cut(s) 104, 516
TaiI ACGT 4 cut(s) 57, 288, 414, 738
TasI AATT 3 cut(s) 297, 752, 858
TatI WGTACW 1 cut(s) 689
TauI GCSGC 6 cut(s) 37, 154, 473, 476, 536, 810
TfiI GAWTC 2 cut(s) 110, 203
Tru1I TTAA 1 cut(s) 861
Tru9I TTAA 1 cut(s) 861
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 2 cut(s) 73, 662
Tsp45I GTSAC 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 67
VspI ATTAAT 1 cut(s) 861
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 3 cut(s) 417, 491, 693
ZraI GACGTC 1 cut(s) 286
ZrmI AGTACT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.