Rw1G023490

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003426
Physical Location & Seq
Reverse (-)
49301867 .. 49303164
1298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023490.1

Sequence Viewer

Length: 801 bp
ATGGTGAGAATTCGTAAGAACCTTCAGGTCTCACCGCTGTTTTTCAGCTCCAACGTAGCTCCGGAAGACTTCTGGCCACATATCTGCCGACTGAATCAATCTCCGTGGGACGTCAATCCCCCTCCAGAGTTGGAAGCTCAGGAAGATTTAAACGTGTATGGGAGCTTGAAAGGATCTATCGGAGCTACAAAAAGCTTTGCGTTGCCATCCAGGATGGATATTGACAAAGTGATTATCGATGTCGAAGAGGATTCAAAGGGGTTTGGTGAAGGCAATGAAATTGGGTTAAAGAAGCAGAAGATATGTAAAAAGAACGATGGAAAAGGGTGGAAGTGTAGAAAGGAAGTGAAGGAAGGGTACTCATTTTGCAATCACCACATAACCCTTATTAGGTCTTACCATAATACTGGCAGCAACAATGCTACTAGCAATGCTAACAAGTCCTTTTCTTTCAAGAAGCCCAAGGTGACCACGAGAGAGGCTCCTGAGGCCAAGGCGAAAAAGGGCTCGTCCTCGAAGTCTAATGACTATATATACTACTCGCTTTTTAGTCCATGGTGGAATCAGAATAGAAGGACTATAGTTGAGGAACTGACCGCGAAGAATATTGCTGTTGAAAATGGTGCTACTACATCCTCTACTAGTGTAGCTACTCCAAATCAAGATCCTCCATCACCCAAGGCTGGTAATCAGAATCAATCCGCTTATGTGGATGTCAATGAAGGCAACGATGATGGGAGCAACAAATGTCGCCGAAAGAGGATGAGGAAGCCTATGAAGGCTGTCCTTATTTCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

266

Amino Acids

29.72

Weight (kDa)

9.42

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 102 - 135 1.6e-09 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 114
AccII CGCG 1 cut(s) 599
AccIII TCCGGA 1 cut(s) 61
AciI CCGC 3 cut(s) 35, 597, 702
AclWI GGATC 2 cut(s) 181, 659
AcoI YGGCCR 1 cut(s) 74
AcsI RAATTY 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 8
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 390, 683
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 4 cut(s) 169, 255, 454, 617
AhlI ACTAGT 1 cut(s) 641
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 8 cut(s) 48, 59, 137, 165, 185, 195, 650, 798
AluI AGCT 8 cut(s) 48, 59, 137, 165, 185, 195, 650, 798
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 2 cut(s) 181, 659
Aor13HI TCCGGA 1 cut(s) 61
AoxI GGCC 2 cut(s) 74, 489
ApeKI GCWGC 1 cut(s) 411
ApoI RAATTY 1 cut(s) 9
AsuHPI GGTGA 6 cut(s) 16, 24, 278, 365, 478, 666
AxyI CCTNAGG 1 cut(s) 486
BalI TGGCCA 1 cut(s) 76
BanII GRGCYC 1 cut(s) 509
BarI GAAGNNNNNNTAC 2 cut(s) 341, 373
BauI CACGAG 1 cut(s) 472
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 423
BccI CCATC 5 cut(s) 208, 214, 311, 679, 728
BciT130I CCWGG 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 34
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 2 cut(s) 426, 642
BfmI CTRYAG 1 cut(s) 579
BisI GCNGC 1 cut(s) 412
BlsI GCNGC 1 cut(s) 413
Bme1390I CCNGG 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 483
BmrFI CCNGG 1 cut(s) 211
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 466, 498
BpmI CTGGAG 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 34
BsaJI CCNNGG 5 cut(s) 104, 462, 492, 554, 678
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 683
Bse1I ACTGG 1 cut(s) 412
Bse21I CCTNAGG 1 cut(s) 486
Bse3DI GCAATG 2 cut(s) 280, 436
BseAI TCCGGA 1 cut(s) 61
BseBI CCWGG 1 cut(s) 211
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 5 cut(s) 104, 462, 492, 554, 678
BseGI GGATG 5 cut(s) 206, 219, 632, 718, 768
BseLI CCNNNNNNNGG 2 cut(s) 390, 683
BseMI GCAATG 2 cut(s) 280, 436
BseMII CTCAG 2 cut(s) 152, 477
BseNI ACTGG 1 cut(s) 412
BseXI GCAGC 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 599
BshFI GGCC 2 cut(s) 76, 491
BshVI ATCGAT 1 cut(s) 237
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 390, 683
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 2 cut(s) 76, 491
Bso31I GGTCTC 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 509
Bsp13I TCCGGA 1 cut(s) 61
Bsp143I GATC 2 cut(s) 173, 664
Bsp19I CCATGG 1 cut(s) 554
BspACI CCGC 3 cut(s) 35, 597, 702
BspANI GGCC 2 cut(s) 76, 491
BspCNI CTCAG 2 cut(s) 151, 478
BspDI ATCGAT 1 cut(s) 237
BspEI TCCGGA 1 cut(s) 61
BspFNI CGCG 1 cut(s) 599
BspLI GGNNCC 1 cut(s) 483
BspPI GGATC 2 cut(s) 181, 659
BspTNI GGTCTC 1 cut(s) 34
BsrDI GCAATG 2 cut(s) 280, 436
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 5 cut(s) 104, 462, 492, 554, 678
BssMI GATC 2 cut(s) 173, 664
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 472
BssT1I CCWWGG 4 cut(s) 462, 492, 554, 678
Bst2BI CACGAG 1 cut(s) 472
Bst2UI CCWGG 1 cut(s) 211
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 1 cut(s) 111
BstDEI CTNAG 2 cut(s) 138, 486
BstDSI CCRYGG 2 cut(s) 104, 554
BstEII GGTNACC 1 cut(s) 466
BstF5I GGATG 5 cut(s) 206, 219, 632, 718, 768
BstFNI CGCG 1 cut(s) 599
BstKTI GATC 2 cut(s) 176, 667
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 2 cut(s) 173, 664
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 1 cut(s) 211
BstPI GGTNACC 1 cut(s) 466
BstSCI CCNGG 1 cut(s) 209
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 1 cut(s) 599
BstV1I GCAGC 1 cut(s) 423
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 2 cut(s) 173, 664
BstXI CCANNNNNNTGG 1 cut(s) 407
BstYI RGATCY 2 cut(s) 173, 664
Bsu15I ATCGAT 1 cut(s) 237
Bsu36I CCTNAGG 1 cut(s) 486
BsuRI GGCC 2 cut(s) 76, 491
BsuTUI ATCGAT 1 cut(s) 237
BtgI CCRYGG 2 cut(s) 104, 554
BtsCI GGATG 5 cut(s) 206, 219, 632, 718, 768
ClaI ATCGAT 1 cut(s) 237
Csp6I GTAC 1 cut(s) 358
CspCI CAANNNNNGTGG 2 cut(s) 86, 121
CviAII CATG 1 cut(s) 555
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 2 cut(s) 138, 486
DpnI GATC 2 cut(s) 175, 666
DpnII GATC 2 cut(s) 173, 664
DraI TTTAAA 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 74
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
Eco130I CCWWGG 4 cut(s) 462, 492, 554, 678
Eco24I GRGCYC 1 cut(s) 509
Eco31I GGTCTC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 8
Eco81I CCTNAGG 1 cut(s) 486
Eco91I GGTNACC 1 cut(s) 466
EcoO65I GGTNACC 1 cut(s) 466
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 1 cut(s) 209
EcoT14I CCWWGG 4 cut(s) 462, 492, 554, 678
EcoT38I GRGCYC 1 cut(s) 509
ErhI CCWWGG 4 cut(s) 462, 492, 554, 678
FaeI CATG 1 cut(s) 558
FaqI GGGAC 1 cut(s) 122
FatI CATG 1 cut(s) 554
Fnu4HI GCNGC 1 cut(s) 412
FokI GGATG 5 cut(s) 193, 226, 619, 725, 775
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 1 cut(s) 412
FspBI CTAG 2 cut(s) 426, 642
GluI GCNGC 1 cut(s) 412
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 2 cut(s) 76, 491
HapII CCGG 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 1 cut(s) 558
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 4 cut(s) 94, 251, 562, 694
HpaII CCGG 1 cut(s) 62
HphI GGTGA 6 cut(s) 16, 24, 278, 365, 478, 666
Hpy188I TCNGA 3 cut(s) 182, 567, 693
Hpy188III TCNNGA 6 cut(s) 62, 125, 140, 454, 485, 662
HpyAV CCTTC 7 cut(s) 32, 263, 343, 347, 567, 716, 772
HpyCH4IV ACGT 3 cut(s) 54, 111, 153
HpyCH4V TGCA 1 cut(s) 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 2 cut(s) 138, 486
HpySE526I ACGT 3 cut(s) 54, 111, 153
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 1 cut(s) 558
Kpn2I TCCGGA 1 cut(s) 61
Kzo9I GATC 2 cut(s) 173, 664
LmnI GCTCC 6 cut(s) 53, 64, 162, 182, 487, 738
Lsp1109I GCAGC 1 cut(s) 423
MaeI CTAG 2 cut(s) 426, 642
MaeII ACGT 3 cut(s) 54, 111, 153
MaeIII GTNAC 1 cut(s) 466
MalI GATC 2 cut(s) 175, 666
MboI GATC 2 cut(s) 173, 664
MboII GAAGA 5 cut(s) 77, 155, 257, 310, 613
MflI RGATCY 2 cut(s) 173, 664
MhlI GDGCHC 1 cut(s) 509
MlsI TGGCCA 1 cut(s) 76
MluCI AATT 2 cut(s) 9, 279
MluNI TGGCCA 1 cut(s) 76
MmeI TCCRAC 2 cut(s) 75, 111
Mox20I TGGCCA 1 cut(s) 76
MroI TCCGGA 1 cut(s) 61
MscI TGGCCA 1 cut(s) 76
MseI TTAA 2 cut(s) 149, 287
Msp20I TGGCCA 1 cut(s) 76
MspA1I CMGCKG 2 cut(s) 37, 798
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 211
MvaI CCWGG 1 cut(s) 211
MvnI CGCG 1 cut(s) 599
MwoI GCNNNNNNNGC 1 cut(s) 488
NcoI CCATGG 1 cut(s) 554
NdeII GATC 2 cut(s) 173, 664
NlaIII CATG 1 cut(s) 558
NlaIV GGNNCC 1 cut(s) 483
NmuCI GTSAC 1 cut(s) 466
PfeI GAWTC 4 cut(s) 94, 251, 562, 694
PfoI TCCNGGA 1 cut(s) 209
PkrI GCNGC 1 cut(s) 413
Psp6I CCWGG 1 cut(s) 209
PspEI GGTNACC 1 cut(s) 466
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 483
PsuI RGATCY 2 cut(s) 173, 664
PvuII CAGCTG 1 cut(s) 798
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SaqAI TTAA 2 cut(s) 149, 287
SatI GCNGC 1 cut(s) 412
Sau3AI GATC 2 cut(s) 173, 664
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 1 cut(s) 509
SfcI CTRYAG 1 cut(s) 579
SpeI ACTAGT 1 cut(s) 641
Sse9I AATT 2 cut(s) 9, 279
SsiI CCGC 3 cut(s) 35, 597, 702
SspI AATATT 1 cut(s) 607
SspMI CTAG 2 cut(s) 426, 642
StyD4I CCNGG 1 cut(s) 209
StyI CCWWGG 4 cut(s) 462, 492, 554, 678
TaiI ACGT 3 cut(s) 57, 114, 156
TaqI TCGA 3 cut(s) 237, 243, 515
TasI AATT 2 cut(s) 9, 279
TfiI GAWTC 4 cut(s) 94, 251, 562, 694
Tru1I TTAA 2 cut(s) 149, 287
Tru9I TTAA 2 cut(s) 149, 287
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 1 cut(s) 411
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 3 cut(s) 291, 735, 791
TspGWI ACGGA 1 cut(s) 93
XapI RAATTY 1 cut(s) 9
XspI CTAG 2 cut(s) 426, 642
ZraI GACGTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.