MD07G1133500.v1.1

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003426
Physical Location & Seq
Forward (+)
19498896 .. 19500878
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1133500.v1.1.491

Sequence Viewer

Length: 879 bp
ATGCGGATCAGGAAGAACGCCAAGGTCTCTCCCCTTCTGTTCACCTCGCACGCTCCAGATGCCGAGGAGCTTCTGCCGCATGTGTGCCAGATCAACCGGAATCCCTGGGATATTATTCCTTTCGGACCGGAGTCGGACCAGTGGGAGGTCGAAGAGAGCTTGTACGGTGGGAGCTTCGGTGATTCAATCGGAGCTGTCGAGAGCGTTGCGTCGTTGAGGGATGGCGCGGACGAGAAACCCTTGGAAAAGAATGAAGGGCCCGTGGCCGAGGGTATCGACATTGAGAACAATTGGAAGGCGGGCGACGAGAGGACGGGGAGCATGTGCTGCCAACAGAATGACGGCCGTGGGTGGCATTGTAAGAAAAAAGCCAAGCAAGGGGAGTCCTTCTGCAACCATCACCTGTCCCTACTTCGATCGAACTGCATTGTTAAGGCCAACGGCAATGGATACTGTAGGGATAATGGAAATAATCATAACATATCTTCGAAGAAGGCTGCCGCCGCGGTGAGCGGAGCGGTACAGCTGAAAAATGGAGGCGGCGGCGGCCGGAAGAAGGTGAAGAAGGAATCCGCCCCTAACCCCTACGAGTTCTATTACTATTCGGGATTTGGCCCGTGGCGGAGTCGGAAGAGAGGCGGCGACAAAGAAAAGGAAGAGACCAAGGCCGAGGGTGAAATTGCTACTACGACTAGCTTTCCTCCTCATCCTCATGAGAGCGCTCATCGGTCACCCTCGTCTCCAATTGCAAATGGGGAATTCGATTATAACGACGAGGATGACGAGGATGACGACGATGAGCTCGATGGCAGCGGCGTTAGCAGCAGGAAGCGGATGAGGAAGCCGGTGAAGGAGAGATCGCTCAAGTCCCTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

293

Amino Acids

32.18

Weight (kDa)

6.03

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 110 - 140 1.1e-06 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 768
AccBSI CCGCTC 2 cut(s) 513, 518
AccII CGCG 2 cut(s) 227, 506
AclWI GGATC 1 cut(s) 14
AcoI YGGCCR 3 cut(s) 264, 343, 547
AcsI RAATTY 1 cut(s) 758
AfaI GTAC 2 cut(s) 164, 522
AfeI AGCGCT 1 cut(s) 721
AfiI CCNNNNNNNGG 3 cut(s) 145, 378, 556
AgsI TTSAA 1 cut(s) 186
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 7 cut(s) 70, 159, 174, 194, 526, 696, 802
AluI AGCT 7 cut(s) 70, 159, 174, 194, 526, 696, 802
Alw21I GWGCWC 1 cut(s) 804
Alw26I GTCTC 3 cut(s) 31, 653, 744
AlwI GGATC 1 cut(s) 14
Aor51HI AGCGCT 1 cut(s) 721
AoxI GGCC 7 cut(s) 257, 264, 343, 435, 547, 613, 666
ApaI GGGCCC 1 cut(s) 261
ApeKI GCWGC 4 cut(s) 327, 497, 810, 822
ApoI RAATTY 1 cut(s) 758
AspLEI GCGC 2 cut(s) 227, 722
AspS9I GGNCC 5 cut(s) 125, 136, 257, 258, 614
AsuHPI GGTGA 8 cut(s) 34, 191, 392, 520, 571, 686, 723, 859
AsuII TTCGAA 1 cut(s) 488
AvaII GGWCC 2 cut(s) 125, 136
BaeGI GKGCMC 1 cut(s) 261
BanII GRGCYC 2 cut(s) 261, 804
Bbv12I GWGCWC 1 cut(s) 804
BbvI GCAGC 4 cut(s) 314, 484, 822, 834
BccI CCATC 3 cut(s) 215, 405, 800
BceAI ACGGC 3 cut(s) 330, 358, 457
BcgI CGANNNNNNTGC 4 cut(s) 188, 222, 405, 439
BciT130I CCWGG 1 cut(s) 106
BciVI GTATCC 1 cut(s) 443
BcoDI GTCTC 3 cut(s) 31, 653, 744
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 1 cut(s) 454
BfoI RGCGCY 1 cut(s) 723
BfuI GTATCC 1 cut(s) 443
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 2 cut(s) 125, 136
BmgT120I GGNCC 5 cut(s) 125, 136, 257, 258, 614
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 49
BoxI GACNNNNGTC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 848
BsaI GGTCTC 2 cut(s) 31, 653
BsaWI WCCGGW 2 cut(s) 96, 127
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 378, 556
Bse118I RCCGGY 1 cut(s) 844
Bse1I ACTGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 451
BseBI CCWGG 1 cut(s) 106
BseGI GGATG 5 cut(s) 226, 706, 784, 793, 840
BseLI CCNNNNNNNGG 3 cut(s) 145, 378, 556
BseMI GCAATG 1 cut(s) 451
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 2 cut(s) 80, 693
BseSI GKGCMC 1 cut(s) 261
BseX3I CGGCCG 2 cut(s) 343, 547
BseXI GCAGC 4 cut(s) 314, 484, 822, 834
Bsh1236I CGCG 2 cut(s) 227, 506
Bsh1285I CGRYCG 3 cut(s) 346, 419, 550
BshFI GGCC 7 cut(s) 259, 266, 345, 437, 549, 615, 668
BsiEI CGRYCG 3 cut(s) 346, 419, 550
BsiHKAI GWGCWC 1 cut(s) 804
BsiSI CCGG 4 cut(s) 97, 128, 550, 845
BslFI GGGAC 2 cut(s) 391, 853
BslI CCNNNNNNNGG 3 cut(s) 145, 378, 556
BsmAI GTCTC 3 cut(s) 31, 653, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 2 cut(s) 391, 853
BsnI GGCC 7 cut(s) 259, 266, 345, 437, 549, 615, 668
Bso31I GGTCTC 2 cut(s) 31, 653
Bsp119I TTCGAA 1 cut(s) 488
Bsp120I GGGCCC 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 261, 804
Bsp143I GATC 4 cut(s) 6, 90, 416, 857
BspANI GGCC 7 cut(s) 259, 266, 345, 437, 549, 615, 668
BspFNI CGCG 2 cut(s) 227, 506
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 1 cut(s) 14
BspT104I TTCGAA 1 cut(s) 488
BspTNI GGTCTC 2 cut(s) 31, 653
BsrBI CCGCTC 2 cut(s) 513, 518
BsrDI GCAATG 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 844
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 844
BssMI GATC 4 cut(s) 6, 90, 416, 857
BssT1I CCWWGG 3 cut(s) 21, 240, 663
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 167, 455
Bst6I CTCTTC 3 cut(s) 147, 626, 651
BstAPI GCANNNNNTGC 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 51, 301
BstDSI CCRYGG 4 cut(s) 261, 346, 504, 617
BstEII GGTNACC 1 cut(s) 729
BstF5I GGATG 5 cut(s) 226, 706, 784, 793, 840
BstFNI CGCG 2 cut(s) 227, 506
BstH2I RGCGCY 1 cut(s) 723
BstHHI GCGC 2 cut(s) 227, 722
BstKTI GATC 4 cut(s) 9, 93, 419, 860
BstMAI GTCTC 3 cut(s) 31, 653, 744
BstMBI GATC 4 cut(s) 6, 90, 416, 857
BstMCI CGRYCG 3 cut(s) 346, 419, 550
BstMWI GCNNNNNNNGC 7 cut(s) 59, 76, 327, 503, 546, 819, 822
BstNI CCWGG 1 cut(s) 106
BstNSI RCATGY 2 cut(s) 83, 325
BstPAI GACNNNNGTC 1 cut(s) 130
BstPI GGTNACC 1 cut(s) 729
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 261
BstUI CGCG 2 cut(s) 227, 506
BstV1I GCAGC 4 cut(s) 314, 484, 822, 834
BstZI CGGCCG 2 cut(s) 343, 547
BsuI GTATCC 1 cut(s) 443
BsuRI GGCC 7 cut(s) 259, 266, 345, 437, 549, 615, 668
BtgI CCRYGG 4 cut(s) 261, 346, 504, 617
BtsCI GGATG 5 cut(s) 226, 706, 784, 793, 840
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 2 cut(s) 51, 301
CciI TCATGA 1 cut(s) 712
CfoI GCGC 2 cut(s) 227, 722
Cfr10I RCCGGY 1 cut(s) 844
Cfr13I GGNCC 5 cut(s) 125, 136, 257, 258, 614
Cfr42I CCGCGG 1 cut(s) 507
CpoI CGGWCCG 1 cut(s) 125
CseI GACGC 1 cut(s) 198
Csp6I GTAC 2 cut(s) 163, 521
CspI CGGWCCG 1 cut(s) 125
CviAII CATG 3 cut(s) 80, 322, 713
CviQI GTAC 2 cut(s) 163, 521
DpnI GATC 4 cut(s) 8, 92, 418, 859
DpnII GATC 4 cut(s) 6, 90, 416, 857
EaeI YGGCCR 3 cut(s) 264, 343, 547
EagI CGGCCG 2 cut(s) 343, 547
Eam1104I CTCTTC 3 cut(s) 147, 626, 651
EarI CTCTTC 3 cut(s) 147, 626, 651
EciI GGCGGA 2 cut(s) 562, 637
Ecl136II GAGCTC 1 cut(s) 802
EclXI CGGCCG 2 cut(s) 343, 547
Eco130I CCWWGG 3 cut(s) 21, 240, 663
Eco24I GRGCYC 2 cut(s) 261, 804
Eco31I GGTCTC 2 cut(s) 31, 653
Eco47I GGWCC 2 cut(s) 125, 136
Eco47III AGCGCT 1 cut(s) 721
Eco52I CGGCCG 2 cut(s) 343, 547
Eco53kI GAGCTC 1 cut(s) 802
Eco91I GGTNACC 1 cut(s) 729
EcoICRI GAGCTC 1 cut(s) 802
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 729
EcoRI GAATTC 1 cut(s) 758
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 3 cut(s) 21, 240, 663
EcoT38I GRGCYC 2 cut(s) 261, 804
ErhI CCWWGG 3 cut(s) 21, 240, 663
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 3 cut(s) 83, 325, 716
FaiI YATR 6 cut(s) 81, 323, 477, 482, 714, 768
FaqI GGGAC 2 cut(s) 391, 853
FatI CATG 3 cut(s) 79, 321, 712
FauI CCCGC 1 cut(s) 292
FokI GGATG 5 cut(s) 233, 693, 791, 800, 847
FriOI GRGCYC 2 cut(s) 261, 804
FspBI CTAG 1 cut(s) 693
GlaI GCGC 2 cut(s) 226, 721
GsuI CTGGAG 1 cut(s) 39
HaeII RGCGCY 1 cut(s) 723
HaeIII GGCC 7 cut(s) 259, 266, 345, 437, 549, 615, 668
HapII CCGG 4 cut(s) 97, 128, 550, 845
HgaI GACGC 1 cut(s) 198
HhaI GCGC 2 cut(s) 227, 722
Hin1II CATG 3 cut(s) 83, 325, 716
Hin6I GCGC 2 cut(s) 225, 720
HinP1I GCGC 2 cut(s) 225, 720
HinfI GANTC 6 cut(s) 100, 131, 182, 383, 569, 625
HpaII CCGG 4 cut(s) 97, 128, 550, 845
HphI GGTGA 8 cut(s) 34, 191, 392, 520, 571, 686, 723, 859
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 4 cut(s) 125, 136, 191, 630
Hpy188III TCNNGA 5 cut(s) 10, 56, 199, 606, 713
Hpy8I GTNNAC 1 cut(s) 42
Hpy99I CGWCG 4 cut(s) 214, 308, 776, 797
HpyAV CCTTC 8 cut(s) 44, 248, 289, 397, 487, 550, 559, 844
HpyCH4III ACNGT 2 cut(s) 167, 455
HpyCH4V TGCA 3 cut(s) 393, 426, 749
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 76, 327, 503, 546, 819, 822
Hsp92II CATG 3 cut(s) 83, 325, 716
HspAI GCGC 2 cut(s) 225, 720
KspI CCGCGG 1 cut(s) 507
Kzo9I GATC 4 cut(s) 6, 90, 416, 857
LmnI GCTCC 6 cut(s) 58, 67, 171, 191, 318, 515
Lsp1109I GCAGC 4 cut(s) 314, 484, 822, 834
LweI GCATC 1 cut(s) 49
MaeI CTAG 1 cut(s) 693
MaeIII GTNAC 1 cut(s) 729
MalI GATC 4 cut(s) 8, 92, 418, 859
MbiI CCGCTC 2 cut(s) 513, 518
MboI GATC 4 cut(s) 6, 90, 416, 857
MboII GAAGA 8 cut(s) 25, 164, 477, 502, 565, 574, 643, 668
MfeI CAATTG 2 cut(s) 289, 744
MhlI GDGCHC 2 cut(s) 261, 804
MluCI AATT 4 cut(s) 289, 678, 744, 758
MlyI GAGTC 3 cut(s) 140, 392, 634
MmeI TCCRAC 2 cut(s) 114, 608
MseI TTAA 1 cut(s) 432
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 3 cut(s) 506, 526, 813
MspI CCGG 4 cut(s) 97, 128, 550, 845
MspR9I CCNGG 1 cut(s) 106
MunI CAATTG 2 cut(s) 289, 744
MvaI CCWGG 1 cut(s) 106
MvnI CGCG 2 cut(s) 227, 506
MwoI GCNNNNNNNGC 7 cut(s) 59, 76, 327, 503, 546, 819, 822
NdeII GATC 4 cut(s) 6, 90, 416, 857
NlaIII CATG 3 cut(s) 83, 325, 716
NlaIV GGNNCC 1 cut(s) 259
NmeAIII GCCGAG 3 cut(s) 88, 292, 694
NmuCI GTSAC 1 cut(s) 729
NspI RCATGY 2 cut(s) 83, 325
NspV TTCGAA 1 cut(s) 488
PagI TCATGA 1 cut(s) 712
PasI CCCWGGG 1 cut(s) 105
PcsI WCGNNNNNNNCGW 5 cut(s) 195, 768, 780, 789, 801
PfeI GAWTC 3 cut(s) 100, 182, 569
Ple19I CGATCG 1 cut(s) 419
PleI GAGTC 3 cut(s) 139, 391, 633
PpsI GAGTC 3 cut(s) 139, 391, 633
PshAI GACNNNNGTC 1 cut(s) 130
PsiI TTATAA 1 cut(s) 768
Psp124BI GAGCTC 1 cut(s) 804
Psp6I CCWGG 1 cut(s) 104
PspEI GGTNACC 1 cut(s) 729
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 1 cut(s) 259
PspOMI GGGCCC 1 cut(s) 257
PspPI GGNCC 5 cut(s) 125, 136, 257, 258, 614
PvuI CGATCG 1 cut(s) 419
PvuII CAGCTG 1 cut(s) 526
RsaI GTAC 2 cut(s) 164, 522
RsaNI GTAC 2 cut(s) 163, 521
RseI CAYNNNNRTG 1 cut(s) 711
Rsr2I CGGWCCG 1 cut(s) 125
RsrII CGGWCCG 1 cut(s) 125
SacI GAGCTC 1 cut(s) 804
SacII CCGCGG 1 cut(s) 507
SaqAI TTAA 1 cut(s) 432
Sau3AI GATC 4 cut(s) 6, 90, 416, 857
Sau96I GGNCC 5 cut(s) 125, 136, 257, 258, 614
SchI GAGTC 3 cut(s) 140, 392, 634
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 2 cut(s) 261, 804
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 454
Sfr303I CCGCGG 1 cut(s) 507
SfuI TTCGAA 1 cut(s) 488
SgrBI CCGCGG 1 cut(s) 507
SinI GGWCC 2 cut(s) 125, 136
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 863
SmoI CTYRAG 1 cut(s) 863
Sse9I AATT 4 cut(s) 289, 678, 744, 758
SspMI CTAG 1 cut(s) 693
SstI GAGCTC 1 cut(s) 804
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 3 cut(s) 21, 240, 663
TaaI ACNGT 2 cut(s) 167, 455
TaqI TCGA 8 cut(s) 150, 198, 276, 415, 419, 488, 762, 804
TaqII GACCGA 1 cut(s) 717
TasI AATT 4 cut(s) 289, 678, 744, 758
TauI GCSGC 8 cut(s) 79, 503, 506, 543, 546, 549, 642, 816
TfiI GAWTC 3 cut(s) 100, 182, 569
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 1 cut(s) 146
TseFI GTSAC 1 cut(s) 729
TseI GCWGC 4 cut(s) 327, 497, 810, 822
Tsp45I GTSAC 1 cut(s) 729
TspDTI ATGAA 1 cut(s) 267
TspRI CASTG 1 cut(s) 146
VpaK11BI GGWCC 2 cut(s) 125, 136
XapI RAATTY 1 cut(s) 758
XceI RCATGY 2 cut(s) 83, 325
XspI CTAG 1 cut(s) 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.