pycom01g09650

WRC

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003426
Physical Location & Seq
Reverse (-)
10491789 .. 10493249
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g09650.1

Sequence Viewer

Length: 909 bp
ATGCGGATCAGGAAGAACGCCAAGGTCTCTCCTCTTCTGTTCACCTCGCACGCTCCAGCTGCCGGGGACCTCCATCCGCACGTGTGCCAGCTCAACCGGAACCCCTGGGATGTTATTCCTTTTGGACCGGAGTCCGACCAGTGGGATGTCGAAGAGAGCTTTTACGGTGGGAGCCTTGGTGATTCAATCGGAGCTGTCGAGAGCGTGGCGTCGATGAGGGATGCCACGGAGGAAAGACGCTTGGAAATGATAGAAGAGCCCGTGGCGGAGGATGTCGAATTCGAAAAGGATTCGAATAAGGCGGAGGGCGAGATGGAGGTGGAAGAAGGGAGGATGGGCAGCATGTGCTGCCAACGGAACGACGGCCGTGGGTGGCAATGCAAGAAAAAAGCCAAGCAAGGGCAGTCCTTCTGCAACCACCACCTCTCCCTACTTCGATCGAACTACATTGTCAAGGCTAACGGGAATGTAAACAGTAGGGATAATGGAAATAATCACAACATTTCTTCGAATAAGGCTCCCGCCGCAGTGAGCGGGGTGGTTCAGCTGGAAAACGGGGGCCGCCGCCGCCGGAAGAAGGCGAAGAAGGAATCCGCCTCTAACCCCTACGAGTTCTATTACTATTCGGGGTTTGGCCCGTGTCGGAACCGGAAGAGAGGCTGCGATAAAGAAAAGGAAGAGACCAAGGCCGAGGGTGAAATTGCTACTAATACTAGCTCTGCTACTCCTCCTCCTCCTCCTCCTCCTCATGAGAGCGGTCCTCGCTCACCCTTGTCTCCAATTGCGAATGGGGAATTCAATTATGACGACGAGGATGATGATGATGATGAGCTCGACGGCAGCGGCGAGAGCGGGAGGAGGCGGATGAGGAAGCCGGTGAAGGAGAGATCGCTCAAGTCCCTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

303

Amino Acids

33.6

Weight (kDa)

5.91

Isoelectric Point (pI)

60.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 116 - 148 3.4e-09 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 534, 756, 852
AclWI GGATC 1 cut(s) 14
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 2 cut(s) 278, 794
AcvI CACGTG 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 209
AfiI CCNNNNNNNGG 4 cut(s) 62, 141, 399, 577
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 2 cut(s) 186, 799
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 7 cut(s) 59, 91, 159, 194, 547, 717, 832
AluI AGCT 7 cut(s) 59, 91, 159, 194, 547, 717, 832
Alw21I GWGCWC 1 cut(s) 834
Alw26I GTCTC 3 cut(s) 31, 674, 780
AlwI GGATC 1 cut(s) 14
AoxI GGCC 4 cut(s) 364, 559, 634, 687
ApeKI GCWGC 5 cut(s) 59, 339, 348, 660, 840
ApoI RAATTY 2 cut(s) 278, 794
AspS9I GGNCC 5 cut(s) 67, 125, 559, 635, 758
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 5 cut(s) 34, 191, 707, 759, 889
AsuII TTCGAA 3 cut(s) 282, 293, 509
AvaII GGWCC 3 cut(s) 67, 125, 758
BanII GRGCYC 2 cut(s) 261, 834
BbrPI CACGTG 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 834
BbvI GCAGC 5 cut(s) 46, 335, 351, 647, 852
BccI CCATC 3 cut(s) 81, 307, 328
BceAI ACGGC 3 cut(s) 351, 379, 853
BciT130I CCWGG 1 cut(s) 106
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 3 cut(s) 31, 674, 780
BfaI CTAG 1 cut(s) 714
Bme1390I CCNGG 2 cut(s) 64, 106
Bme18I GGWCC 3 cut(s) 67, 125, 758
BmgT120I GGNCC 5 cut(s) 67, 125, 559, 635, 758
BmiI GGNNCC 6 cut(s) 68, 101, 173, 519, 560, 647
BmrFI CCNGG 2 cut(s) 64, 106
BmsI GCATC 1 cut(s) 211
BoxI GACNNNNGTC 1 cut(s) 130
BplI GAGNNNNNCTC 2 cut(s) 745, 777
BpmI CTGGAG 1 cut(s) 39
Bpu14I TTCGAA 3 cut(s) 282, 293, 509
BpuEI CTTGAG 1 cut(s) 878
BpuMI CCSGG 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 209
BsaI GGTCTC 2 cut(s) 31, 674
BsaWI WCCGGW 3 cut(s) 96, 127, 648
BsaXI ACNNNNNCTCC 6 cut(s) 163, 193, 410, 440, 715, 745
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 141, 399, 577
Bse118I RCCGGY 1 cut(s) 874
Bse1I ACTGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 383
BseBI CCWGG 1 cut(s) 106
BseGI GGATG 8 cut(s) 73, 115, 151, 226, 277, 339, 820, 870
BseLI CCNNNNNNNGG 4 cut(s) 62, 141, 399, 577
BseMI GCAATG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 9 cut(s) 21, 717, 720, 723, 726, 729, 732, 735, 871
BseX3I CGGCCG 1 cut(s) 364
BseXI GCAGC 5 cut(s) 46, 335, 351, 647, 852
Bsh1285I CGRYCG 2 cut(s) 367, 440
BshFI GGCC 4 cut(s) 366, 561, 636, 689
BsiEI CGRYCG 2 cut(s) 367, 440
BsiHKAI GWGCWC 1 cut(s) 834
BsiSI CCGG 6 cut(s) 63, 97, 128, 571, 649, 875
BslFI GGGAC 2 cut(s) 80, 883
BslI CCNNNNNNNGG 4 cut(s) 62, 141, 399, 577
BsmAI GTCTC 3 cut(s) 31, 674, 780
BsmFI GGGAC 2 cut(s) 80, 883
BsnI GGCC 4 cut(s) 366, 561, 636, 689
Bso31I GGTCTC 2 cut(s) 31, 674
Bsp119I TTCGAA 3 cut(s) 282, 293, 509
Bsp1286I GDGCHC 2 cut(s) 261, 834
Bsp143I GATC 3 cut(s) 6, 437, 887
BspANI GGCC 4 cut(s) 366, 561, 636, 689
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 6 cut(s) 68, 101, 173, 519, 560, 647
BspPI GGATC 1 cut(s) 14
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 3 cut(s) 282, 293, 509
BspTNI GGTCTC 2 cut(s) 31, 674
BsrBI CCGCTC 3 cut(s) 534, 756, 852
BsrDI GCAATG 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 874
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 874
BssMI GATC 3 cut(s) 6, 437, 887
BssNI GRCGYC 1 cut(s) 209
BssT1I CCWWGG 3 cut(s) 21, 175, 684
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 167, 476
Bst6I CTCTTC 5 cut(s) 39, 147, 249, 647, 672
BstACI GRCGYC 1 cut(s) 209
BstAPI GCANNNNNTGC 2 cut(s) 345, 348
BstBAI YACGTR 1 cut(s) 82
BstBI TTCGAA 3 cut(s) 282, 293, 509
BstC8I GCNNGC 2 cut(s) 51, 89
BstDSI CCRYGG 3 cut(s) 225, 261, 367
BstF5I GGATG 8 cut(s) 73, 115, 151, 226, 277, 339, 820, 870
BstKTI GATC 3 cut(s) 9, 440, 890
BstMAI GTCTC 3 cut(s) 31, 674, 780
BstMBI GATC 3 cut(s) 6, 437, 887
BstMCI CGRYCG 2 cut(s) 367, 440
BstMWI GCNNNNNNNGC 7 cut(s) 59, 345, 348, 524, 567, 762, 849
BstNI CCWGG 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 346
BstPAI GACNNNNGTC 1 cut(s) 130
BstSCI CCNGG 2 cut(s) 62, 104
BstV1I GCAGC 5 cut(s) 46, 335, 351, 647, 852
BstZI CGGCCG 1 cut(s) 364
BsuRI GGCC 4 cut(s) 366, 561, 636, 689
BtgI CCRYGG 3 cut(s) 225, 261, 367
BtsCI GGATG 8 cut(s) 73, 115, 151, 226, 277, 339, 820, 870
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 146, 534
Cac8I GCNNGC 2 cut(s) 51, 89
CciI TCATGA 1 cut(s) 748
Cfr10I RCCGGY 1 cut(s) 874
Cfr13I GGNCC 5 cut(s) 67, 125, 559, 635, 758
CseI GACGC 2 cut(s) 198, 246
CviAII CATG 2 cut(s) 343, 749
DpnI GATC 3 cut(s) 8, 439, 889
DpnII GATC 3 cut(s) 6, 437, 887
EaeI YGGCCR 1 cut(s) 364
EagI CGGCCG 1 cut(s) 364
Eam1104I CTCTTC 5 cut(s) 39, 147, 249, 647, 672
EarI CTCTTC 5 cut(s) 39, 147, 249, 647, 672
EciI GGCGGA 4 cut(s) 281, 317, 583, 877
Ecl136II GAGCTC 1 cut(s) 832
EclXI CGGCCG 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 21, 175, 684
Eco24I GRGCYC 2 cut(s) 261, 834
Eco31I GGTCTC 2 cut(s) 31, 674
Eco47I GGWCC 3 cut(s) 67, 125, 758
Eco52I CGGCCG 1 cut(s) 364
Eco53kI GAGCTC 1 cut(s) 832
Eco72I CACGTG 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 832
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 2 cut(s) 278, 794
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 3 cut(s) 21, 175, 684
EcoT38I GRGCYC 2 cut(s) 261, 834
ErhI CCWWGG 3 cut(s) 21, 175, 684
FaeI CATG 2 cut(s) 346, 752
FaiI YATR 3 cut(s) 344, 750, 804
FaqI GGGAC 2 cut(s) 80, 883
FatI CATG 2 cut(s) 342, 748
FauI CCCGC 3 cut(s) 527, 529, 845
FokI GGATG 8 cut(s) 60, 122, 158, 233, 284, 346, 827, 877
FriOI GRGCYC 2 cut(s) 261, 834
FspBI CTAG 1 cut(s) 714
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 4 cut(s) 366, 561, 636, 689
HapII CCGG 6 cut(s) 63, 97, 128, 571, 649, 875
HgaI GACGC 2 cut(s) 198, 246
Hin1I GRCGYC 1 cut(s) 209
Hin1II CATG 2 cut(s) 346, 752
HinfI GANTC 4 cut(s) 131, 182, 290, 590
HpaII CCGG 6 cut(s) 63, 97, 128, 571, 649, 875
HphI GGTGA 5 cut(s) 34, 191, 707, 759, 889
Hpy166II GTNNAC 2 cut(s) 42, 472
Hpy188I TCNGA 3 cut(s) 136, 191, 645
Hpy188III TCNNGA 3 cut(s) 10, 199, 749
Hpy8I GTNNAC 2 cut(s) 42, 472
Hpy99I CGWCG 4 cut(s) 214, 365, 812, 839
HpyAV CCTTC 5 cut(s) 320, 418, 571, 580, 874
HpyCH4III ACNGT 2 cut(s) 167, 476
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 381, 414
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 345, 348, 524, 567, 762, 849
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 209
Hsp92II CATG 2 cut(s) 346, 752
Kzo9I GATC 3 cut(s) 6, 437, 887
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 4 cut(s) 58, 171, 191, 523
Lsp1109I GCAGC 5 cut(s) 46, 335, 351, 647, 852
LweI GCATC 1 cut(s) 211
MaeI CTAG 1 cut(s) 714
MaeII ACGT 1 cut(s) 81
MalI GATC 3 cut(s) 8, 439, 889
MbiI CCGCTC 3 cut(s) 534, 756, 852
MboI GATC 3 cut(s) 6, 437, 887
MfeI CAATTG 1 cut(s) 780
MhlI GDGCHC 2 cut(s) 261, 834
MluCI AATT 5 cut(s) 278, 699, 780, 794, 799
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 2 cut(s) 159, 623
MspA1I CMGCKG 3 cut(s) 59, 547, 843
MspI CCGG 6 cut(s) 63, 97, 128, 571, 649, 875
MspR9I CCNGG 2 cut(s) 64, 106
MunI CAATTG 1 cut(s) 780
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 7 cut(s) 59, 345, 348, 524, 567, 762, 849
NciI CCSGG 1 cut(s) 64
NdeII GATC 3 cut(s) 6, 437, 887
NlaIII CATG 2 cut(s) 346, 752
NlaIV GGNNCC 6 cut(s) 68, 101, 173, 519, 560, 647
NmeAIII GCCGAG 1 cut(s) 715
NspI RCATGY 1 cut(s) 346
NspV TTCGAA 3 cut(s) 282, 293, 509
PagI TCATGA 1 cut(s) 748
PasI CCCWGGG 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 843
PfeI GAWTC 3 cut(s) 182, 290, 590
Ple19I CGATCG 1 cut(s) 440
PleI GAGTC 1 cut(s) 139
PmaCI CACGTG 1 cut(s) 82
PmlI CACGTG 1 cut(s) 82
PpsI GAGTC 1 cut(s) 139
Ppu21I YACGTR 1 cut(s) 82
PpuMI RGGWCCY 1 cut(s) 67
PshAI GACNNNNGTC 1 cut(s) 130
Psp124BI GAGCTC 1 cut(s) 834
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 104
PspCI CACGTG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 6 cut(s) 68, 101, 173, 519, 560, 647
PspPI GGNCC 5 cut(s) 67, 125, 559, 635, 758
PspPPI RGGWCCY 1 cut(s) 67
PvuI CGATCG 1 cut(s) 440
PvuII CAGCTG 2 cut(s) 59, 547
SacI GAGCTC 1 cut(s) 834
SapI GCTCTTC 1 cut(s) 249
Sau3AI GATC 3 cut(s) 6, 437, 887
Sau96I GGNCC 5 cut(s) 67, 125, 559, 635, 758
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 64, 106
SduI GDGCHC 2 cut(s) 261, 834
SfaNI GCATC 1 cut(s) 211
SfuI TTCGAA 3 cut(s) 282, 293, 509
SinI GGWCC 3 cut(s) 67, 125, 758
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
Sse9I AATT 5 cut(s) 278, 699, 780, 794, 799
SspMI CTAG 1 cut(s) 714
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 2 cut(s) 62, 104
StyI CCWWGG 3 cut(s) 21, 175, 684
TaaI ACNGT 2 cut(s) 167, 476
TaiI ACGT 1 cut(s) 84
TasI AATT 5 cut(s) 278, 699, 780, 794, 799
TauI GCSGC 5 cut(s) 527, 564, 567, 570, 846
TfiI GAWTC 3 cut(s) 182, 290, 590
TscAI CASTG 2 cut(s) 146, 534
TseI GCWGC 5 cut(s) 59, 339, 348, 660, 840
TspGWI ACGGA 2 cut(s) 242, 370
TspRI CASTG 2 cut(s) 146, 534
VpaK11BI GGWCC 3 cut(s) 67, 125, 758
XapI RAATTY 2 cut(s) 278, 794
XceI RCATGY 1 cut(s) 346
XspI CTAG 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.