Rroxscaffold_4G00299120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003426
Physical Location & Seq
Reverse (-)
19054302 .. 19055693
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299120.1

Sequence Viewer

Length: 828 bp
ATGGTCAGGATCCGCAAGAACTTGAAGGTGTCGCCGCTCCAGTTCTTCCCCAACGTAGCTCCTGAGGACCTGCAGCCCCACATCTGCAAGCTGAACCAGTCTCCTTGGGATTCGAGCCCCTGCCCTTTCGACCTCCTCCTCGACTCGTCGCCGCTCCAGTTCGAAGCTGGGGAGGGTTATATCGGAAATGGGAGCTTGGGGGATTCTATTGCAGCTGTCGAGAGCGTTGCGTCGCCGATGGATATCGACAAGGCAGAAATGGCGACCGGCGATGTGTTGGATAACGTCGACAGAGCAATTGTGAGGTGTCAGAAGAACGACGGCAGGAGCTGGCAATGCAAGAAGGAAGCGAAAGAAGGCTTCTTATTTTGTGACCATCATATCCGAAGGTCGTCCTATGAAGGACACGGCGGTGGCCCCGGCCAAGACGCTAGCCGGAACCCGCCGTGCCAGACCCAAGGCGGTGAAGAAGGGGCGTCGTCGGCGAACCCGTATGAGTTCTACTACTATTCGGGGTTCGGCCCAAAATGGGGCCGAAAGAGAGGCAAGAAAGGCGAGGTGGTGGAGGAGGAGGAGGAGGAGGAGGAGGCCAATAATATTGAGTCAGCAGCTGGAAAGGTTGCAACTTCTTGTAGTACTAGTAGTGCTAACACCGATCCCCAACCTTCTTCACCTCACAACGTTGGTAATGGGGATCAATTGGACTACATGGAAGACGACTTCGACGACGAGGATGGGAGCGAAGAAGAAGGCGGCCCGAGGAGGATGAGGAGGCCGGTGAAGGCTCGATCCTTGATGTCCATAATTAATGTAAAAGCTCAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRC

Protein Analysis

275

Amino Acids

30.12

Weight (kDa)

4.74

Isoelectric Point (pI)

74.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 101 - 131 8.6e-09 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 78
AccBSI CCGCTC 2 cut(s) 37, 154
AccI GTMKAC 1 cut(s) 288
AciI CCGC 7 cut(s) 13, 35, 152, 411, 443, 462, 753
AclI AACGTT 1 cut(s) 681
AclWI GGATC 5 cut(s) 4, 17, 650, 702, 783
AcoI YGGCCR 1 cut(s) 421
AcyI GRCGYC 1 cut(s) 476
AfaI GTAC 1 cut(s) 637
AfiI CCNNNNNNNGG 2 cut(s) 529, 530
AgsI TTSAA 1 cut(s) 25
AhlI ACTAGT 1 cut(s) 638
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 8 cut(s) 59, 91, 167, 195, 215, 330, 611, 818
AluI AGCT 8 cut(s) 59, 91, 167, 195, 215, 330, 611, 818
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 5 cut(s) 4, 17, 650, 702, 783
AlwNI CAGNNNCTG 2 cut(s) 330, 611
Ama87I CYCGRG 1 cut(s) 757
AoxI GGCC 7 cut(s) 415, 421, 520, 532, 588, 754, 773
ApeKI GCWGC 3 cut(s) 73, 212, 608
AseI ATTAAT 1 cut(s) 807
AspS9I GGNCC 5 cut(s) 67, 416, 521, 532, 755
AsuC2I CCSGG 1 cut(s) 420
AsuHPI GGTGA 3 cut(s) 476, 663, 790
AsuII TTCGAA 1 cut(s) 162
AsuNHI GCTAGC 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 757
AvaII GGWCC 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 63
BamHI GGATCC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 720
BbvI GCAGC 3 cut(s) 85, 224, 620
BccI CCATC 3 cut(s) 232, 384, 728
BceAI ACGGC 3 cut(s) 337, 424, 430
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BcnI CCSGG 1 cut(s) 420
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 638
BfaI CTAG 2 cut(s) 432, 639
BfmI CTRYAG 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 78
BisI GCNGC 6 cut(s) 35, 74, 152, 213, 609, 754
BlsI GCNGC 6 cut(s) 36, 75, 153, 214, 610, 755
BmcAI AGTACT 1 cut(s) 637
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 757
BmgT120I GGNCC 5 cut(s) 67, 416, 521, 532, 755
BmiI GGNNCC 4 cut(s) 11, 418, 440, 533
BmrFI CCNGG 1 cut(s) 420
BmtI GCTAGC 1 cut(s) 435
BpiI GAAGAC 1 cut(s) 720
BpmI CTGGAG 2 cut(s) 23, 140
Bpu14I TTCGAA 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 804
BpuMI CCSGG 1 cut(s) 420
BsaBI GATNNNNATC 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 476
BsaJI CCNNGG 4 cut(s) 104, 418, 457, 758
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 530
Bse118I RCCGGY 2 cut(s) 266, 775
Bse1I ACTGG 3 cut(s) 40, 97, 157
Bse21I CCTNAGG 1 cut(s) 63
Bse3DI GCAATG 1 cut(s) 341
Bse8I GATNNNNATC 1 cut(s) 242
BseDI CCNNGG 4 cut(s) 104, 418, 457, 758
BseGI GGATG 2 cut(s) 739, 771
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 529, 530
BseMI GCAATG 1 cut(s) 341
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 3 cut(s) 40, 97, 157
BseXI GCAGC 3 cut(s) 85, 224, 620
BseYI CCCAGC 1 cut(s) 167
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 7 cut(s) 417, 423, 522, 534, 590, 756, 775
BsiEI CGRYCG 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 757
BsiSI CCGG 4 cut(s) 267, 420, 436, 776
BslI CCNNNNNNNGG 2 cut(s) 529, 530
BsmAI GTCTC 1 cut(s) 105
BsnI GGCC 7 cut(s) 417, 423, 522, 534, 590, 756, 775
BsoBI CYCGRG 1 cut(s) 757
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 4 cut(s) 9, 655, 694, 788
BspACI CCGC 7 cut(s) 13, 35, 152, 411, 443, 462, 753
BspANI GGCC 7 cut(s) 417, 423, 522, 534, 590, 756, 775
BspCNI CTCAG 1 cut(s) 55
BspLI GGNNCC 4 cut(s) 11, 418, 440, 533
BspMAI CTGCAG 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 78
BspOI GCTAGC 1 cut(s) 435
BspPI GGATC 5 cut(s) 4, 17, 650, 702, 783
BspT104I TTCGAA 1 cut(s) 162
BsrBI CCGCTC 2 cut(s) 37, 154
BsrDI GCAATG 1 cut(s) 341
BsrFI RCCGGY 2 cut(s) 266, 775
BsrI ACTGG 3 cut(s) 40, 97, 157
BssAI RCCGGY 2 cut(s) 266, 775
BssECI CCNNGG 4 cut(s) 104, 418, 457, 758
BssMI GATC 4 cut(s) 9, 655, 694, 788
BssNI GRCGYC 1 cut(s) 476
BssT1I CCWWGG 2 cut(s) 104, 457
BstACI GRCGYC 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 162
BstC8I GCNNGC 3 cut(s) 89, 332, 433
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 739, 771
BstKTI GATC 4 cut(s) 12, 658, 697, 791
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 4 cut(s) 9, 655, 694, 788
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 4 cut(s) 260, 336, 482, 552
BstSCI CCNGG 1 cut(s) 418
BstSFI CTRYAG 1 cut(s) 71
BstV1I GCAGC 3 cut(s) 85, 224, 620
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 63
BsuRI GGCC 7 cut(s) 417, 423, 522, 534, 590, 756, 775
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 2 cut(s) 739, 771
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 3 cut(s) 89, 332, 433
CaiI CAGNNNCTG 2 cut(s) 330, 611
Cfr10I RCCGGY 2 cut(s) 266, 775
Cfr13I GGNCC 5 cut(s) 67, 416, 521, 532, 755
CseI GACGC 3 cut(s) 219, 437, 465
Csp6I GTAC 1 cut(s) 636
CviAII CATG 1 cut(s) 709
CviQI GTAC 1 cut(s) 636
DdeI CTNAG 1 cut(s) 63
DpnI GATC 4 cut(s) 11, 657, 696, 790
DpnII GATC 4 cut(s) 9, 655, 694, 788
EaeI YGGCCR 1 cut(s) 421
Eco130I CCWWGG 2 cut(s) 104, 457
Eco24I GRGCYC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 67
Eco81I CCTNAGG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 757
EcoO109I RGGNCCY 1 cut(s) 67
EcoRV GATATC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 104, 457
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 2 cut(s) 104, 457
FaeI CATG 1 cut(s) 712
FaiI YATR 6 cut(s) 180, 381, 399, 495, 710, 803
FatI CATG 1 cut(s) 708
FauI CCCGC 1 cut(s) 450
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 6 cut(s) 35, 74, 152, 213, 609, 754
FokI GGATG 2 cut(s) 746, 778
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 6 cut(s) 35, 74, 152, 213, 609, 754
FspBI CTAG 2 cut(s) 432, 639
GluI GCNGC 6 cut(s) 35, 74, 152, 213, 609, 754
GsaI CCCAGC 1 cut(s) 171
GsuI CTGGAG 2 cut(s) 23, 140
HaeIII GGCC 7 cut(s) 417, 423, 522, 534, 590, 756, 775
HapII CCGG 4 cut(s) 267, 420, 436, 776
HgaI GACGC 3 cut(s) 219, 437, 465
Hin1I GRCGYC 1 cut(s) 476
Hin1II CATG 1 cut(s) 712
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 4 cut(s) 110, 143, 203, 602
HpaII CCGG 4 cut(s) 267, 420, 436, 776
HphI GGTGA 3 cut(s) 476, 663, 790
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 3 cut(s) 185, 312, 386
Hpy188III TCNNGA 4 cut(s) 7, 62, 220, 821
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 8 cut(s) 151, 235, 290, 323, 481, 484, 728, 731
HpyAV CCTTC 9 cut(s) 19, 337, 350, 381, 395, 464, 675, 743, 775
HpyCH4IV ACGT 3 cut(s) 54, 285, 681
HpyCH4V TGCA 5 cut(s) 73, 87, 212, 339, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 336, 482, 552
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 3 cut(s) 54, 285, 681
Hsp92I GRCGYC 1 cut(s) 476
Hsp92II CATG 1 cut(s) 712
Kzo9I GATC 4 cut(s) 9, 655, 694, 788
LmnI GCTCC 6 cut(s) 42, 64, 159, 192, 327, 738
Lsp1109I GCAGC 3 cut(s) 85, 224, 620
MaeI CTAG 2 cut(s) 432, 639
MaeII ACGT 3 cut(s) 54, 285, 681
MaeIII GTNAC 1 cut(s) 371
MalI GATC 4 cut(s) 11, 657, 696, 790
MbiI CCGCTC 2 cut(s) 37, 154
MboI GATC 4 cut(s) 9, 655, 694, 788
MboII GAAGA 7 cut(s) 37, 325, 479, 660, 725, 755, 758
MfeI CAATTG 2 cut(s) 297, 698
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 3 cut(s) 297, 698, 804
MlyI GAGTC 2 cut(s) 137, 611
MmeI TCCRAC 1 cut(s) 258
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 1 cut(s) 411
MspA1I CMGCKG 2 cut(s) 215, 611
MspI CCGG 4 cut(s) 267, 420, 436, 776
MspR9I CCNGG 1 cut(s) 420
MunI CAATTG 2 cut(s) 297, 698
MwoI GCNNNNNNNGC 4 cut(s) 260, 336, 482, 552
NciI CCSGG 1 cut(s) 420
NdeII GATC 4 cut(s) 9, 655, 694, 788
NheI GCTAGC 1 cut(s) 431
NlaIII CATG 1 cut(s) 712
NlaIV GGNNCC 4 cut(s) 11, 418, 440, 533
NmuCI GTSAC 1 cut(s) 371
NspV TTCGAA 1 cut(s) 162
OliI CACNNNNGTG 1 cut(s) 411
PcsI WCGNNNNNNNCGW 2 cut(s) 488, 723
PfeI GAWTC 2 cut(s) 110, 203
PkrI GCNGC 6 cut(s) 36, 75, 153, 214, 610, 755
PleI GAGTC 2 cut(s) 137, 610
PpsI GAGTC 2 cut(s) 137, 610
PpuMI RGGWCCY 1 cut(s) 67
PshBI ATTAAT 1 cut(s) 807
Psp1406I AACGTT 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 167
PspN4I GGNNCC 4 cut(s) 11, 418, 440, 533
PspPI GGNCC 5 cut(s) 67, 416, 521, 532, 755
PspPPI RGGWCCY 1 cut(s) 67
PstI CTGCAG 1 cut(s) 75
PstNI CAGNNNCTG 2 cut(s) 330, 611
PsuI RGATCY 1 cut(s) 9
PvuII CAGCTG 2 cut(s) 215, 611
RsaI GTAC 1 cut(s) 637
RsaNI GTAC 1 cut(s) 636
RseI CAYNNNNRTG 1 cut(s) 411
SalI GTCGAC 1 cut(s) 287
SaqAI TTAA 1 cut(s) 807
SatI GCNGC 6 cut(s) 35, 74, 152, 213, 609, 754
Sau3AI GATC 4 cut(s) 9, 655, 694, 788
Sau96I GGNCC 5 cut(s) 67, 416, 521, 532, 755
ScaI AGTACT 1 cut(s) 637
SchI GAGTC 2 cut(s) 137, 611
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 71
SfuI TTCGAA 1 cut(s) 162
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 819
SmoI CTYRAG 1 cut(s) 819
SpeI ACTAGT 1 cut(s) 638
Sse9I AATT 3 cut(s) 297, 698, 804
SsiI CCGC 7 cut(s) 13, 35, 152, 411, 443, 462, 753
SspI AATATT 1 cut(s) 598
SspMI CTAG 2 cut(s) 432, 639
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 2 cut(s) 104, 457
TaiI ACGT 3 cut(s) 57, 288, 684
TaqI TCGA 9 cut(s) 113, 129, 141, 162, 219, 246, 288, 723, 787
TasI AATT 3 cut(s) 297, 698, 804
TatI WGTACW 1 cut(s) 635
TauI GCSGC 3 cut(s) 37, 154, 756
TfiI GAWTC 2 cut(s) 110, 203
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 3 cut(s) 73, 212, 608
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 67
VspI ATTAAT 1 cut(s) 807
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 2 cut(s) 432, 639
ZrmI AGTACT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.