Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005244

Physical Location & Seq

Genomic Coordinates Forward (+)

20194854 .. 20198191

Gene Structure 3338 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1089100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1230 bp

ATGATGGAACCACAAAATAATCAACATGCACAGGTTATTGAAATCCATCGAGATGTTCACGATCGTGAAACGAGTAATAGTGGGAGTAAGATTTGTGGGGAAGCACCATGTGGACTTGCGGATGTTGGATCCAACTCTAAAGATGCCAAAGAACGATCAGCCTCCATGCGCAAGCTTTTAATAGCAGTGGTACTTTGTGTTCTGTTCATGGCTGTTGAGGTGGTTGGTGGCATTGAAGCCAATAGTTTAGCAATATTGACAGATGCAGCACATTTGCTTTCGGATGTTGCAGCATTTGCCATCTCCTTGTTCTCTTTGTGGGCGGCTGGCTGGGAAGCCACTCCCCGTCAGTCTTACGGGTTTTTTAGGATTGAAATTCTTGGTGCCCTGGTTTCCATCCAGCTCATATGGTTGCTTGCCGGGATATTGGTATATGAAGCCATAGTTAGAATGATACATGACACAGGTGAGGTGAATGGATTTCTAATGTTTCTCGTTGCCGCATTTGGTCTAGTGGTTAATATAGCTATGGCCATACTGTTAGGCCATGATCATGGGCATGGACATGGACATGATCACGGTGATCACAGTCACGGAATGACTATTTCTGCTCATCACCATCACCACGATCATGCTCACGAGAAACACTCGCATGGTCATGATCATGAGGTGCACTCACATGATCATGAGCACCCTCACACTCATGAAGATGATCATGAACACCACCATGCCGACAAGGATCTTGCTGAGCCACTGTTGGATAAGCCGAAAGATGGGCAAGTAGAGAAGAAGCAAAGGAACATAAATCTACAGGGTGCTTATCTCCATGTGCTTGGAGACTCGGTACAAAGTATTGGGGTAATGATCGGCGGGGCAATCATATGGGCCAAGCCGGAGTGGAAGATAGTTGATTTGATCTGCACCCTAATTTTTTCGGTCATTGTTCTGGGGACAACTATTAAGATGATGCGGAACATTCTTGATGTCCTGATGGAGAGCACGCCGAGAGAGATTGATGCAACGAGACTTGAAACCGGACTGTTGGAGATGGAGGAAGTGGTAGCAGTCCACGAGCTGCATATATGGGCTATCACAGTGGGGAAAGTCCTGCTGGCTTGCCATGTGAAAGTTAGACCAGAAGCAAATGCAGACATGGTGCTGGAGGACGTAATAGCTTATATCAGAAGGGAGTATAACATCAGCCATGTCACGATCCAAATCGAGCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0000325 GO:0003674 GO:0005215 GO:0005385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0006873 GO:0006875 GO:0006882 GO:0008150 GO:0008324 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0015075 GO:0015318 GO:0016020 GO:0019725 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0031090 GO:0032879 GO:0034220 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072503 GO:0072507 GO:0072509 GO:0072511 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

410

Amino Acids

45.37

Weight (kDa)

5.81

Isoelectric Point (pI)

28.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	59 - 331	2.2e-36	Cation efflux transmembrane domain
ZT_dimer	PF16916	335 - 408	1.6e-12	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46800 AT2G46800 AT2G46800 AT2G46800 AT2G46800 AT3G61940
fragaria_vesca	FvH4_7g17780
malus_domestica	MD01G1089100.v1.1 MD07G1160600.v1.1
prunus_persica	Prupe.2G198700_v2.0.a1 Prupe.2G198700_v2.0.a1
pyrus_communis	pycom01g11340 pycom07g15660
rosa_chinensis	RchiOBHm_Chr1g0361171
rosa_laevigata	RLG00000027742 RLG00000027743
rosa_multiflora	Rmu_sc0001816.1_g000005 Rmu_sc0001816.1_g000011 Rmu_sc0007111.1_g000001 Rmu_sc0007111.1_g000012
rosa_roxburghii	Rroxscaffold_4G00295300 Rroxscaffold_4G00295320
rosa_rugosa	Rorug01G0287200
rosa_samantha	Rh1AG298000 Rh1BG262000 Rh1CG279400 Rh1DG291900
rosa_wichuraiana	Rw1G026340 Rw1G026350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	170
AccB1I	GGYRCC	1 cut(s)	383
AciI	CCGC	5 cut(s)	119, 323, 501, 870, 970
AclWI	GGATC	4 cut(s)	123, 136, 747, 1207
AcoI	YGGCCR	1 cut(s)	531
AcsI	RAATTY	1 cut(s)	375
AdeI	CACNNNGTG	1 cut(s)	110
AfaI	GTAC	2 cut(s)	192, 846
AfiI	CCNNNNNNNGG	1 cut(s)	773
AgsI	TTSAA	4 cut(s)	41, 236, 374, 1031
AjnI	CCWGG	1 cut(s)	387
AleI	CACNNNNGTG	1 cut(s)	63
AluBI	AGCT	5 cut(s)	175, 403, 527, 1075, 1175
AluI	AGCT	5 cut(s)	175, 403, 527, 1075, 1175
Alw21I	GWGCWC	3 cut(s)	675, 693, 1001
Alw26I	GTCTC	2 cut(s)	831, 1018
Alw44I	GTGCAC	1 cut(s)	671
AlwI	GGATC	4 cut(s)	123, 136, 747, 1207
AoxI	GGCC	3 cut(s)	531, 544, 885
ApaLI	GTGCAC	1 cut(s)	671
ApeKI	GCWGC	3 cut(s)	266, 290, 1075
ApoI	RAATTY	1 cut(s)	375
AspLEI	GCGC	1 cut(s)	171
AspS9I	GGNCC	1 cut(s)	885
AsuC2I	CCSGG	1 cut(s)	421
AsuHPI	GGTGA	5 cut(s)	479, 484, 593, 608, 614
BaeGI	GKGCMC	2 cut(s)	388, 675
BalI	TGGCCA	1 cut(s)	533
BamHI	GGATCC	1 cut(s)	128
BanI	GGYRCC	1 cut(s)	383
BauI	CACGAG	2 cut(s)	638, 1070
Bbv12I	GWGCWC	3 cut(s)	675, 693, 1001
BbvI	GCAGC	3 cut(s)	278, 302, 1062
BccI	CCATC	7 cut(s)	54, 308, 404, 627, 767, 985, 1042
BciT130I	CCWGG	1 cut(s)	389
BclI	TGATCA	6 cut(s)	550, 574, 583, 661, 682, 712
BcnI	CCSGG	1 cut(s)	421
BcoDI	GTCTC	2 cut(s)	831, 1018
BfaI	CTAG	1 cut(s)	512
BfmI	CTRYAG	1 cut(s)	809
BisI	GCNGC	5 cut(s)	267, 291, 324, 501, 1076
BlpI	GCTNAGC	1 cut(s)	747
BlsI	GCNGC	5 cut(s)	268, 292, 325, 502, 1077
Bme1390I	CCNGG	2 cut(s)	389, 421
BmgT120I	GGNCC	1 cut(s)	885
BmiI	GGNNCC	3 cut(s)	9, 130, 385
BmrFI	CCNGG	2 cut(s)	389, 421
BmsI	GCATC	4 cut(s)	133, 253, 957, 1006
BplI	GAGNNNNNCTC	4 cut(s)	632, 664, 659, 691
BpmI	CTGGAG	1 cut(s)	1181
Bpu1102I	GCTNAGC	1 cut(s)	747
BpuMI	CCSGG	1 cut(s)	421
BsaBI	GATNNNNATC	1 cut(s)	1217
BsaJI	CCNNGG	1 cut(s)	387
BsaWI	WCCGGW	1 cut(s)	1034
BsaXI	ACNNNNNCTCC	2 cut(s)	828, 858
Bsc4I	CCNNNNNNNGG	1 cut(s)	773
Bse8I	GATNNNNATC	1 cut(s)	1217
BseBI	CCWGG	1 cut(s)	389
BseDI	CCNNGG	1 cut(s)	387
BseGI	GGATG	3 cut(s)	127, 289, 396
BseJI	GATNNNNATC	1 cut(s)	1217
BseLI	CCNNNNNNNGG	1 cut(s)	773
BseMII	CTCAG	1 cut(s)	738
BseSI	GKGCMC	2 cut(s)	388, 675
BseXI	GCAGC	3 cut(s)	278, 302, 1062
BseYI	CCCAGC	1 cut(s)	330
BsgI	GTGCAG	1 cut(s)	904
Bsh1285I	CGRYCG	1 cut(s)	64
BshFI	GGCC	3 cut(s)	533, 546, 887
BshNI	GGYRCC	1 cut(s)	383
BsiEI	CGRYCG	1 cut(s)	64
BsiHKAI	GWGCWC	3 cut(s)	675, 693, 1001
BsiSI	CCGG	3 cut(s)	420, 893, 1035
BslFI	GGGAC	1 cut(s)	964
BslI	CCNNNNNNNGG	1 cut(s)	773
BsmAI	GTCTC	2 cut(s)	831, 1018
BsmFI	GGGAC	1 cut(s)	964
BsnI	GGCC	3 cut(s)	533, 546, 887
Bsp1286I	GDGCHC	4 cut(s)	388, 675, 693, 1001
Bsp1720I	GCTNAGC	1 cut(s)	747
BspACI	CCGC	5 cut(s)	119, 323, 501, 870, 970
BspANI	GGCC	3 cut(s)	533, 546, 887
BspCNI	CTCAG	1 cut(s)	739
BspHI	TCATGA	5 cut(s)	658, 664, 685, 703, 715
BspLI	GGNNCC	3 cut(s)	9, 130, 385
BspPI	GGATC	4 cut(s)	123, 136, 747, 1207
BspT107I	GGYRCC	1 cut(s)	383
BssECI	CCNNGG	1 cut(s)	387
BssSI	CACGAG	2 cut(s)	638, 1070
Bst2BI	CACGAG	2 cut(s)	638, 1070
Bst2UI	CCWGG	1 cut(s)	389
Bst4CI	ACNGT	6 cut(s)	540, 581, 590, 756, 1041, 1096
BstAPI	GCANNNNNTGC	1 cut(s)	296
BstC8I	GCNNGC	6 cut(s)	173, 328, 417, 1001, 1113, 1117
BstDEI	CTNAG	1 cut(s)	747
BstF5I	GGATG	3 cut(s)	127, 289, 396
BstHHI	GCGC	1 cut(s)	171
BstMAI	GTCTC	2 cut(s)	831, 1018
BstMCI	CGRYCG	1 cut(s)	64
BstMWI	GCNNNNNNNGC	1 cut(s)	296
BstNI	CCWGG	1 cut(s)	389
BstNSI	RCATGY	1 cut(s)	29
BstSCI	CCNGG	2 cut(s)	387, 419
BstSFI	CTRYAG	1 cut(s)	809
BstSLI	GKGCMC	2 cut(s)	388, 675
BstV1I	GCAGC	3 cut(s)	278, 302, 1062
BstX2I	RGATCY	2 cut(s)	128, 739
BstXI	CCANNNNNNTGG	2 cut(s)	554, 833
BstYI	RGATCY	2 cut(s)	128, 739
BsuRI	GGCC	3 cut(s)	533, 546, 887
BtsCI	GGATG	3 cut(s)	127, 289, 396
BtsI	GCAGTG	1 cut(s)	192
BtsIMutI	CAGTG	3 cut(s)	192, 752, 1101
Cac8I	GCNNGC	6 cut(s)	173, 328, 417, 1001, 1113, 1117
CciI	TCATGA	5 cut(s)	658, 664, 685, 703, 715
CfoI	GCGC	1 cut(s)	171
Cfr13I	GGNCC	1 cut(s)	885
Csp6I	GTAC	2 cut(s)	191, 845
CviQI	GTAC	2 cut(s)	191, 845
DdeI	CTNAG	1 cut(s)	747
DraIII	CACNNNGTG	1 cut(s)	110
EaeI	YGGCCR	1 cut(s)	531
EcoRII	CCWGG	1 cut(s)	387
FaqI	GGGAC	1 cut(s)	964
FauI	CCCGC	1 cut(s)	863
FauNDI	CATATG	2 cut(s)	407, 881
FbaI	TGATCA	6 cut(s)	550, 574, 583, 661, 682, 712
Fnu4HI	GCNGC	5 cut(s)	267, 291, 324, 501, 1076
FokI	GGATG	3 cut(s)	134, 296, 383
Fsp4HI	GCNGC	5 cut(s)	267, 291, 324, 501, 1076
FspBI	CTAG	1 cut(s)	512
FspI	TGCGCA	1 cut(s)	170
GlaI	GCGC	1 cut(s)	170
GluI	GCNGC	5 cut(s)	267, 291, 324, 501, 1076
GsaI	CCCAGC	1 cut(s)	334
GsuI	CTGGAG	1 cut(s)	1181
HaeIII	GGCC	3 cut(s)	533, 546, 887
HapII	CCGG	3 cut(s)	420, 893, 1035
HhaI	GCGC	1 cut(s)	171
Hin6I	GCGC	1 cut(s)	169
HinP1I	GCGC	1 cut(s)	169
HindIII	AAGCTT	1 cut(s)	173
HinfI	GANTC	1 cut(s)	839
HpaII	CCGG	3 cut(s)	420, 893, 1035
HphI	GGTGA	5 cut(s)	479, 484, 593, 608, 614
Hpy166II	GTNNAC	4 cut(s)	58, 113, 673, 1069
Hpy188I	TCNGA	2 cut(s)	283, 1184
Hpy8I	GTNNAC	4 cut(s)	58, 113, 673, 1069
HpyAV	CCTTC	1 cut(s)	1179
HpyCH4III	ACNGT	6 cut(s)	540, 581, 590, 756, 1041, 1096
HpyCH4IV	ACGT	1 cut(s)	1167
HpyCH4V	TGCA	8 cut(s)	29, 266, 290, 673, 921, 1019, 1078, 1148
HpyF10VI	GCNNNNNNNGC	1 cut(s)	296
HpyF3I	CTNAG	1 cut(s)	747
HpySE526I	ACGT	1 cut(s)	1167
HspAI	GCGC	1 cut(s)	169
Ksp22I	TGATCA	6 cut(s)	550, 574, 583, 661, 682, 712
Lsp1109I	GCAGC	3 cut(s)	278, 302, 1062
LweI	GCATC	4 cut(s)	133, 253, 957, 1006
MaeI	CTAG	1 cut(s)	512
MaeII	ACGT	1 cut(s)	1167
MaeIII	GTNAC	2 cut(s)	590, 1207
MboII	GAAGA	3 cut(s)	719, 799, 913
MflI	RGATCY	2 cut(s)	128, 739
MhlI	GDGCHC	4 cut(s)	388, 675, 693, 1001
MlsI	TGGCCA	1 cut(s)	533
MluCI	AATT	2 cut(s)	375, 927
MluNI	TGGCCA	1 cut(s)	533
MlyI	GAGTC	1 cut(s)	833
MmeI	TCCRAC	4 cut(s)	106, 156, 738, 1023
MnlI	CCTC	7 cut(s)	172, 211, 463, 661, 705, 1045, 1156
Mox20I	TGGCCA	1 cut(s)	533
MscI	TGGCCA	1 cut(s)	533
MseI	TTAA	3 cut(s)	179, 519, 960
Msp20I	TGGCCA	1 cut(s)	533
MspI	CCGG	3 cut(s)	420, 893, 1035
MspR9I	CCNGG	2 cut(s)	389, 421
MvaI	CCWGG	1 cut(s)	389
MwoI	GCNNNNNNNGC	1 cut(s)	296
NciI	CCSGG	1 cut(s)	421
NdeI	CATATG	2 cut(s)	407, 881
NlaIV	GGNNCC	3 cut(s)	9, 130, 385
NmeAIII	GCCGAG	1 cut(s)	1029
NmuCI	GTSAC	2 cut(s)	590, 1207
NsbI	TGCGCA	1 cut(s)	170
NspI	RCATGY	1 cut(s)	29
OliI	CACNNNNGTG	1 cut(s)	63
PagI	TCATGA	5 cut(s)	658, 664, 685, 703, 715
PkrI	GCNGC	5 cut(s)	268, 292, 325, 502, 1077
Ple19I	CGATCG	1 cut(s)	64
PleI	GAGTC	1 cut(s)	833
PpsI	GAGTC	1 cut(s)	833
Psp6I	CCWGG	1 cut(s)	387
PspFI	CCCAGC	1 cut(s)	330
PspGI	CCWGG	1 cut(s)	387
PspN4I	GGNNCC	3 cut(s)	9, 130, 385
PspPI	GGNCC	1 cut(s)	885
PsrI	GAACNNNNNNTAC	2 cut(s)	183, 215
PsuI	RGATCY	2 cut(s)	128, 739
PvuI	CGATCG	1 cut(s)	64
RsaI	GTAC	2 cut(s)	192, 846
RsaNI	GTAC	2 cut(s)	191, 845
SaqAI	TTAA	3 cut(s)	179, 519, 960
SatI	GCNGC	5 cut(s)	267, 291, 324, 501, 1076
Sau96I	GGNCC	1 cut(s)	885
SchI	GAGTC	1 cut(s)	833
ScrFI	CCNGG	2 cut(s)	389, 421
SduI	GDGCHC	4 cut(s)	388, 675, 693, 1001
SfaNI	GCATC	4 cut(s)	133, 253, 957, 1006
SfcI	CTRYAG	1 cut(s)	809
Sse9I	AATT	2 cut(s)	375, 927
SsiI	CCGC	5 cut(s)	119, 323, 501, 870, 970
SspI	AATATT	1 cut(s)	255
SspMI	CTAG	1 cut(s)	512
StyD4I	CCNGG	2 cut(s)	387, 419
TaaI	ACNGT	6 cut(s)	540, 581, 590, 756, 1041, 1096
TaiI	ACGT	1 cut(s)	1170
TaqI	TCGA	2 cut(s)	49, 1221
TaqII	GACCGA	1 cut(s)	925
TasI	AATT	2 cut(s)	375, 927
TauI	GCSGC	2 cut(s)	326, 503
Tru1I	TTAA	3 cut(s)	179, 519, 960
Tru9I	TTAA	3 cut(s)	179, 519, 960
TscAI	CASTG	3 cut(s)	192, 759, 1101
TseFI	GTSAC	2 cut(s)	590, 1207
TseI	GCWGC	3 cut(s)	266, 290, 1075
Tsp45I	GTSAC	2 cut(s)	590, 1207
TspDTI	ATGAA	4 cut(s)	196, 450, 720, 732
TspGWI	ACGGA	1 cut(s)	609
TspRI	CASTG	3 cut(s)	192, 759, 1101
VneI	GTGCAC	1 cut(s)	671
XapI	RAATTY	1 cut(s)	375
XceI	RCATGY	1 cut(s)	29
XspI	CTAG	1 cut(s)	512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.