Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0005244

Physical Location & Seq

Genomic Coordinates Forward (+)

23478514 .. 23481852

Gene Structure 3339 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1160600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGGGGTTCTTCTATTTCTTTATCTGCTGTGTTATGGAAGCACAAAATACTCAACATGCACAGGTTATTGAGATCCATCAAGATGTTCATGATGGTGAGACGAGTTATGGTGGGAGTAAGATTTGTGGGGAAGCACCATGTGGACTCGCGGATGTTGGATCCAACTCTAAAGATGCCAAAGAACGATCAGCTTCCATGCGCAAACTCTTAATAGCAGTGGTACTGTGTGTTCTGTTCATGGCTGTTGAAGTAGTTGGTGGCATTGAAGCAAATAGTTTAGCAATATTGACAGATGCAGCACATTTGCTTTCGGATGTTGCAGCATTTGCCATCTCCTTGTTCGCTCTGTGGGCGGCTAGCTGGGAAGCCACTCCCCGCCAGTCTTACGGGTTTTTTAGGATTGAAATTCTCGGTGCCCTGGTATCCATCCAACTCATATGGTTGCTTGCCGGGATATTGGTATATGAAGCCATAGTTAGAATGATACATAACACAAGCGAGGTGAATGGATTTCTAATGTTTCTCGTTGCCGCATTTGGTCTAGTGGTTAATATAGTTATGGCCATACTATTAGGTCATGATCATGGACATGGACATGATGGACATGATCACGGTGGTCATAGTCATGGAATGACTGTTTCTTCTCATCATGATCATCACGATCATGCTCTCGAGAATCACTCGCATGATCATGATCATGAGGAGCACTCACATGATGATGAGCACCTTCATGAAGATGATCATAAACACCATCATGCCGACAAAGATCTTGCTGAGCCACTGTTGGATAAGCCAAAAGATGGACAAGGAGAGAAGAAGCAACGGAGCATAAATCTACAGGGAGCTTATCTCCATGTGCTTGGAGACTCCATTCAAAGTATCGGGGTAATGATTGGAGGGGCAATCATATGGGTCAAGCCGGAGTGGAAGATAGTCGATTTAATCTGCACCCTAATCTTTTCGGTCATTGTTTTGGGGACAACTATCAATATGATGCGGAACATTCTTGATGTCCTGATGGAGAGCACTCCGAGAGAGATTGATGCATCGAAACTTGAAACGGGACTGTTGGAGATGGACGAAGTGGTAGCTGTCCACGAACTACATATATGGGCTATCACAGTGGGGAAAATCCTGCTGGCTTGCCATGTGAAAGTTAGACCAGAAGCAAATGCAGACATGGTGCTGGACAACGTAATAGCTTATATCAGAAGGGAGTATAACATCAGCCATGTCACGATACAAATCGAGCGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0000325 GO:0003674 GO:0005215 GO:0005385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0006873 GO:0006875 GO:0006882 GO:0008150 GO:0008324 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0015075 GO:0015318 GO:0016020 GO:0019725 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0031090 GO:0032879 GO:0034220 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072503 GO:0072507 GO:0072509 GO:0072511 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

419

Amino Acids

46.27

Weight (kDa)

5.52

Isoelectric Point (pI)

30.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	69 - 340	3e-43	Cation efflux transmembrane domain
ZT_dimer	PF16916	344 - 417	7e-13	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46800 AT2G46800 AT2G46800 AT2G46800 AT2G46800 AT3G61940
fragaria_vesca	FvH4_7g17780
malus_domestica	MD01G1089100.v1.1 MD07G1160600.v1.1
prunus_persica	Prupe.2G198700_v2.0.a1 Prupe.2G198700_v2.0.a1
pyrus_communis	pycom01g11340 pycom07g15660
rosa_chinensis	RchiOBHm_Chr1g0361171
rosa_laevigata	RLG00000027742 RLG00000027743
rosa_multiflora	Rmu_sc0001816.1_g000005 Rmu_sc0001816.1_g000011 Rmu_sc0007111.1_g000001 Rmu_sc0007111.1_g000012
rosa_roxburghii	Rroxscaffold_4G00295300 Rroxscaffold_4G00295320
rosa_rugosa	Rorug01G0287200
rosa_samantha	Rh1AG298000 Rh1BG262000 Rh1CG279400 Rh1DG291900
rosa_wichuraiana	Rw1G026340 Rw1G026350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	200
AccB1I	GGYRCC	1 cut(s)	413
AccB7I	CCANNNNNTGG	1 cut(s)	800
AccII	CGCG	1 cut(s)	149
AciI	CCGC	5 cut(s)	149, 353, 376, 531, 997
AclWI	GGATC	3 cut(s)	67, 153, 166
AcoI	YGGCCR	1 cut(s)	561
AcsI	RAATTY	1 cut(s)	405
AdeI	CACNNNGTG	1 cut(s)	140
AfaI	GTAC	1 cut(s)	222
AfeI	AGCGCT	1 cut(s)	1253
AfiI	CCNNNNNNNGG	1 cut(s)	800
AgsI	TTSAA	5 cut(s)	248, 266, 404, 875, 1058
AjnI	CCWGG	1 cut(s)	417
AluBI	AGCT	5 cut(s)	191, 360, 845, 1091, 1202
AluI	AGCT	5 cut(s)	191, 360, 845, 1091, 1202
Alw21I	GWGCWC	3 cut(s)	708, 726, 1028
Alw26I	GTCTC	2 cut(s)	92, 858
AlwI	GGATC	3 cut(s)	67, 153, 166
Ama87I	CYCGRG	1 cut(s)	671
Aor51HI	AGCGCT	1 cut(s)	1253
AoxI	GGCC	1 cut(s)	561
ApeKI	GCWGC	2 cut(s)	296, 320
ApoI	RAATTY	1 cut(s)	405
AspLEI	GCGC	2 cut(s)	201, 1254
AsuC2I	CCSGG	1 cut(s)	451
AsuHPI	GGTGA	2 cut(s)	107, 514
AsuNHI	GCTAGC	1 cut(s)	356
AvaI	CYCGRG	1 cut(s)	671
BaeGI	GKGCMC	1 cut(s)	418
BalI	TGGCCA	1 cut(s)	563
BamHI	GGATCC	1 cut(s)	158
BanI	GGYRCC	1 cut(s)	413
Bbv12I	GWGCWC	3 cut(s)	708, 726, 1028
BbvI	GCAGC	2 cut(s)	308, 332
BccI	CCATC	9 cut(s)	84, 86, 338, 434, 593, 759, 794, 1012, 1069
BciT130I	CCWGG	1 cut(s)	419
BciVI	GTATCC	1 cut(s)	433
BclI	TGATCA	6 cut(s)	580, 607, 652, 688, 694, 739
BcnI	CCSGG	1 cut(s)	451
BcoDI	GTCTC	2 cut(s)	92, 858
BfaI	CTAG	3 cut(s)	357, 542, 1255
BfmI	CTRYAG	1 cut(s)	836
BfoI	RGCGCY	1 cut(s)	1255
BfuI	GTATCC	1 cut(s)	433
BglII	AGATCT	1 cut(s)	766
BisI	GCNGC	4 cut(s)	297, 321, 354, 531
BlpI	GCTNAGC	1 cut(s)	774
BlsI	GCNGC	4 cut(s)	298, 322, 355, 532
Bme1390I	CCNGG	2 cut(s)	419, 451
BmeT110I	CYCGRG	1 cut(s)	671
BmiI	GGNNCC	2 cut(s)	160, 415
BmrFI	CCNGG	2 cut(s)	419, 451
BmsI	GCATC	5 cut(s)	163, 283, 984, 1033, 1055
BmtI	GCTAGC	1 cut(s)	360
BplI	GAGNNNNNCTC	6 cut(s)	665, 697, 692, 724, 834, 866
Bpu1102I	GCTNAGC	1 cut(s)	774
BpuMI	CCSGG	1 cut(s)	451
BsaBI	GATNNNNATC	2 cut(s)	693, 1244
BsaJI	CCNNGG	1 cut(s)	417
Bsc4I	CCNNNNNNNGG	1 cut(s)	800
Bse1I	ACTGG	1 cut(s)	379
Bse8I	GATNNNNATC	2 cut(s)	693, 1244
BseBI	CCWGG	1 cut(s)	419
BseDI	CCNNGG	1 cut(s)	417
BseGI	GGATG	3 cut(s)	157, 319, 426
BseJI	GATNNNNATC	2 cut(s)	693, 1244
BseLI	CCNNNNNNNGG	1 cut(s)	800
BseMII	CTCAG	1 cut(s)	765
BseNI	ACTGG	1 cut(s)	379
BseRI	GAGGAG	1 cut(s)	716
BseSI	GKGCMC	1 cut(s)	418
BseXI	GCAGC	2 cut(s)	308, 332
BseYI	CCCAGC	1 cut(s)	360
BsgI	GTGCAG	1 cut(s)	931
Bsh1236I	CGCG	1 cut(s)	149
BshFI	GGCC	1 cut(s)	563
BshNI	GGYRCC	1 cut(s)	413
BsiHKAI	GWGCWC	3 cut(s)	708, 726, 1028
BsiHKCI	CYCGRG	1 cut(s)	671
BsiSI	CCGG	2 cut(s)	450, 920
BslFI	GGGAC	2 cut(s)	991, 1077
BslI	CCNNNNNNNGG	1 cut(s)	800
BsmAI	GTCTC	2 cut(s)	92, 858
BsmBI	CGTCTC	1 cut(s)	92
BsmFI	GGGAC	2 cut(s)	991, 1077
BsnI	GGCC	1 cut(s)	563
BsoBI	CYCGRG	1 cut(s)	671
Bsp1286I	GDGCHC	4 cut(s)	418, 708, 726, 1028
Bsp1720I	GCTNAGC	1 cut(s)	774
BspACI	CCGC	5 cut(s)	149, 353, 376, 531, 997
BspANI	GGCC	1 cut(s)	563
BspCNI	CTCAG	1 cut(s)	766
BspFNI	CGCG	1 cut(s)	149
BspHI	TCATGA	6 cut(s)	88, 577, 649, 691, 697, 730
BspLI	GGNNCC	2 cut(s)	160, 415
BspOI	GCTAGC	1 cut(s)	360
BspPI	GGATC	3 cut(s)	67, 153, 166
BspT107I	GGYRCC	1 cut(s)	413
BsrI	ACTGG	1 cut(s)	379
BssECI	CCNNGG	1 cut(s)	417
Bst2UI	CCWGG	1 cut(s)	419
Bst4CI	ACNGT	6 cut(s)	225, 614, 637, 783, 1068, 1123
BstAPI	GCANNNNNTGC	1 cut(s)	326
BstC8I	GCNNGC	4 cut(s)	358, 447, 1140, 1144
BstDEI	CTNAG	1 cut(s)	774
BstF5I	GGATG	3 cut(s)	157, 319, 426
BstFNI	CGCG	1 cut(s)	149
BstH2I	RGCGCY	1 cut(s)	1255
BstHHI	GCGC	2 cut(s)	201, 1254
BstMAI	GTCTC	2 cut(s)	92, 858
BstMWI	GCNNNNNNNGC	2 cut(s)	326, 350
BstNI	CCWGG	1 cut(s)	419
BstNSI	RCATGY	1 cut(s)	59
BstSCI	CCNGG	2 cut(s)	417, 449
BstSFI	CTRYAG	1 cut(s)	836
BstSLI	GKGCMC	1 cut(s)	418
BstUI	CGCG	1 cut(s)	149
BstV1I	GCAGC	2 cut(s)	308, 332
BstX2I	RGATCY	3 cut(s)	72, 158, 766
BstXI	CCANNNNNNTGG	1 cut(s)	860
BstYI	RGATCY	3 cut(s)	72, 158, 766
BsuI	GTATCC	1 cut(s)	433
BsuRI	GGCC	1 cut(s)	563
BtsCI	GGATG	3 cut(s)	157, 319, 426
BtsI	GCAGTG	1 cut(s)	222
BtsIMutI	CAGTG	3 cut(s)	222, 779, 1128
Cac8I	GCNNGC	4 cut(s)	358, 447, 1140, 1144
CciI	TCATGA	6 cut(s)	88, 577, 649, 691, 697, 730
CfoI	GCGC	2 cut(s)	201, 1254
Csp6I	GTAC	1 cut(s)	221
CviQI	GTAC	1 cut(s)	221
DdeI	CTNAG	1 cut(s)	774
DraIII	CACNNNGTG	1 cut(s)	140
EaeI	YGGCCR	1 cut(s)	561
Eco47III	AGCGCT	1 cut(s)	1253
Eco88I	CYCGRG	1 cut(s)	671
EcoRII	CCWGG	1 cut(s)	417
EcoT22I	ATGCAT	1 cut(s)	1048
Esp3I	CGTCTC	1 cut(s)	92
FaqI	GGGAC	2 cut(s)	991, 1077
FauI	CCCGC	1 cut(s)	383
FauNDI	CATATG	2 cut(s)	437, 908
FbaI	TGATCA	6 cut(s)	580, 607, 652, 688, 694, 739
Fnu4HI	GCNGC	4 cut(s)	297, 321, 354, 531
FokI	GGATG	3 cut(s)	164, 326, 413
Fsp4HI	GCNGC	4 cut(s)	297, 321, 354, 531
FspBI	CTAG	3 cut(s)	357, 542, 1255
FspI	TGCGCA	1 cut(s)	200
GlaI	GCGC	2 cut(s)	200, 1253
GluI	GCNGC	4 cut(s)	297, 321, 354, 531
GsaI	CCCAGC	1 cut(s)	364
HaeII	RGCGCY	1 cut(s)	1255
HaeIII	GGCC	1 cut(s)	563
HapII	CCGG	2 cut(s)	450, 920
HhaI	GCGC	2 cut(s)	201, 1254
Hin6I	GCGC	2 cut(s)	199, 1252
HinP1I	GCGC	2 cut(s)	199, 1252
HinfI	GANTC	3 cut(s)	144, 676, 866
HpaII	CCGG	2 cut(s)	450, 920
HphI	GGTGA	2 cut(s)	107, 514
Hpy166II	GTNNAC	2 cut(s)	143, 1096
Hpy188I	TCNGA	3 cut(s)	313, 1032, 1211
Hpy8I	GTNNAC	2 cut(s)	143, 1096
HpyAV	CCTTC	2 cut(s)	737, 1206
HpyCH4III	ACNGT	6 cut(s)	225, 614, 637, 783, 1068, 1123
HpyCH4IV	ACGT	1 cut(s)	1194
HpyCH4V	TGCA	6 cut(s)	59, 296, 320, 948, 1046, 1175
HpyF10VI	GCNNNNNNNGC	2 cut(s)	326, 350
HpyF3I	CTNAG	1 cut(s)	774
HpySE526I	ACGT	1 cut(s)	1194
HspAI	GCGC	2 cut(s)	199, 1252
Ksp22I	TGATCA	6 cut(s)	580, 607, 652, 688, 694, 739
LmnI	GCTCC	3 cut(s)	703, 825, 842
Lsp1109I	GCAGC	2 cut(s)	308, 332
LweI	GCATC	5 cut(s)	163, 283, 984, 1033, 1055
MaeI	CTAG	3 cut(s)	357, 542, 1255
MaeII	ACGT	1 cut(s)	1194
MaeIII	GTNAC	1 cut(s)	1234
MboII	GAAGA	4 cut(s)	633, 746, 826, 940
MflI	RGATCY	3 cut(s)	72, 158, 766
MhlI	GDGCHC	4 cut(s)	418, 708, 726, 1028
MlsI	TGGCCA	1 cut(s)	563
MluCI	AATT	1 cut(s)	405
MluNI	TGGCCA	1 cut(s)	563
MlyI	GAGTC	2 cut(s)	138, 860
MmeI	TCCRAC	5 cut(s)	136, 186, 454, 765, 1050
MnlI	CCTC	3 cut(s)	493, 694, 890
Mox20I	TGGCCA	1 cut(s)	563
Mph1103I	ATGCAT	1 cut(s)	1048
MscI	TGGCCA	1 cut(s)	563
MseI	TTAA	3 cut(s)	209, 549, 941
Msp20I	TGGCCA	1 cut(s)	563
MspI	CCGG	2 cut(s)	450, 920
MspR9I	CCNGG	2 cut(s)	419, 451
MvaI	CCWGG	1 cut(s)	419
MvnI	CGCG	1 cut(s)	149
MwoI	GCNNNNNNNGC	2 cut(s)	326, 350
NciI	CCSGG	1 cut(s)	451
NdeI	CATATG	2 cut(s)	437, 908
NheI	GCTAGC	1 cut(s)	356
NlaIV	GGNNCC	2 cut(s)	160, 415
NmuCI	GTSAC	1 cut(s)	1234
NsbI	TGCGCA	1 cut(s)	200
NsiI	ATGCAT	1 cut(s)	1048
NspI	RCATGY	1 cut(s)	59
PaeR7I	CTCGAG	1 cut(s)	671
PagI	TCATGA	6 cut(s)	88, 577, 649, 691, 697, 730
PfeI	GAWTC	1 cut(s)	676
PflMI	CCANNNNNTGG	1 cut(s)	800
PkrI	GCNGC	4 cut(s)	298, 322, 355, 532
PleI	GAGTC	2 cut(s)	138, 860
PpsI	GAGTC	2 cut(s)	138, 860
Psp6I	CCWGG	1 cut(s)	417
PspFI	CCCAGC	1 cut(s)	360
PspGI	CCWGG	1 cut(s)	417
PspN4I	GGNNCC	2 cut(s)	160, 415
PsrI	GAACNNNNNNTAC	2 cut(s)	213, 245
PsuI	RGATCY	3 cut(s)	72, 158, 766
RsaI	GTAC	1 cut(s)	222
RsaNI	GTAC	1 cut(s)	221
SaqAI	TTAA	3 cut(s)	209, 549, 941
SatI	GCNGC	4 cut(s)	297, 321, 354, 531
SchI	GAGTC	2 cut(s)	138, 860
ScrFI	CCNGG	2 cut(s)	419, 451
SduI	GDGCHC	4 cut(s)	418, 708, 726, 1028
SfaNI	GCATC	5 cut(s)	163, 283, 984, 1033, 1055
SfcI	CTRYAG	1 cut(s)	836
Sfr274I	CTCGAG	1 cut(s)	671
SlaI	CTCGAG	1 cut(s)	671
SmlI	CTYRAG	1 cut(s)	671
SmoI	CTYRAG	1 cut(s)	671
Sse9I	AATT	1 cut(s)	405
SsiI	CCGC	5 cut(s)	149, 353, 376, 531, 997
SspI	AATATT	1 cut(s)	285
SspMI	CTAG	3 cut(s)	357, 542, 1255
StyD4I	CCNGG	2 cut(s)	417, 449
TaaI	ACNGT	6 cut(s)	225, 614, 637, 783, 1068, 1123
TaiI	ACGT	1 cut(s)	1197
TaqI	TCGA	4 cut(s)	672, 936, 1049, 1248
TaqII	GACCGA	1 cut(s)	952
TasI	AATT	1 cut(s)	405
TauI	GCSGC	2 cut(s)	356, 533
TfiI	GAWTC	1 cut(s)	676
Tru1I	TTAA	3 cut(s)	209, 549, 941
Tru9I	TTAA	3 cut(s)	209, 549, 941
TscAI	CASTG	3 cut(s)	222, 786, 1128
TseFI	GTSAC	1 cut(s)	1234
TseI	GCWGC	2 cut(s)	296, 320
Tsp45I	GTSAC	1 cut(s)	1234
TspDTI	ATGAA	5 cut(s)	77, 226, 480, 719, 747
TspGWI	ACGGA	1 cut(s)	838
TspRI	CASTG	3 cut(s)	222, 786, 1128
Van91I	CCANNNNNTGG	1 cut(s)	800
XapI	RAATTY	1 cut(s)	405
XceI	RCATGY	1 cut(s)	59
XhoI	CTCGAG	1 cut(s)	671
XspI	CTAG	3 cut(s)	357, 542, 1255
Zsp2I	ATGCAT	1 cut(s)	1048

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.