Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004474

Physical Location & Seq

Genomic Coordinates Reverse (-)

23157053 .. 23162389

Gene Structure 5337 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1117800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGCTTGTTGCTTGCTTTTGCTTCCGCTTGACTGTATTTTGGTTGCTAAAGTGCACAGGGGTGAGAGGGTTGGAGATTCAGGGGAACTTCGTGAAGTTCACGGCGATTGGACTATACTTGGAGGATAACGTCGTACCTCTGCTCGCCGTTAAGTGGAAGGGTAAGACGGCAGAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGACATTGTTACAGTCGGAATTTACACTGATGCAAAAGAAAAGGCCATTGAAAAGTTCCTTGAGGTCTTCAACGATCAAAATTTCCTGCCCGGTGCCTCCATTCTTTTCACGCAATCCCCCAAGGGATCACTGACGATGGCTCCAACGCCATCGGTCGCCGGACTCCAGGTCGAGACGACTGCGTTTCCACCGTCCGTCAAACCTCCGGGCTCCTCCAACACTCTGTTCCTCGGCGGCGCAGGGGTGAGGGGGCTGGAGATTCAGGGGAACTTCGTGAAGTTCACGGCGATCGGAGTGTACTTGGAGGATAACGTCGTGCCTCAGCTCGCCGTTAAGTGGAAGGGTAAGACGGCCGAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGACATCGTTACAGGTCCGTTTGAGAAATTCATTCAAGTGACAACGATACTGCCACTGACAGGCCAGCAATACTCTCACAAAGTTTCGGAGAATTGCGTTGCCTTTTGGAAATCAATCGGAATTTACACTGATGCAGAAGGCAAAGCCATTGAAAAGTTCCTTGAGGTCTTCAAAGATCAAAACTTCCCACCCGGCGCCTCCATTCTTTTCACGCAATCTCCCAAAGGATCACTAATGATTAGCTTCTCTAGAGATGCATCCGTACCTGAAGCTGCAAACACGGTGATAGAAAACAAACTACTTTCCGATGCAGTTCTAGAGTCGATCGTTGGAAAGCACGGTGTTTCCCCTGCAGCAAAGCAAAGTTTGGCCGGAAGGTTATCCGAATTGTTGAACGGGTGTAATGAATCTAATGATGCCAAAGCTGGAAATGAGAAAGATTGTCAAATAGCTCCACCACCGTCGCTCGCCGGACTTCAGGTCGAAACGACTGCGTTTCCACCGTCCGTCAAACCTCCGGGCTCCCCCAACACTCTGTTCCTCGGCGGGGTGAGGGGGCTGGAGATTCAGGGGAACTTCGTGAAGTTCACGGCAATCGGAGTGTACTTGGAGGATAACGCAGTGCCTCAGCTCGCCGTTAAGTGGAAGGGTAAGACAGCCAAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGACATCGTTACAGGTCCATTTGAGAAATTCATTCAAGTGACAACGATACTGCCACTGACAGGCCAGCAATACTCTGAGAAAGTTTCGGAGAATTGCATTGCCTTTTGGAAGTTAGTCGGAATTTACACGGATGCGGAAGGCAAAGCCATTGAAATGTTCCTTGAGGTTTTCAAAGATCAAAATTTCCCACCCGGCGCCTCCATTCTTTTCACACAATCTCCCAAAGGATCACTAACGATTAGCTTCTCTAGAGATGCATCCGTACCTGAAGCTGCAAACGTGGTGATAGAAAACAAACTACTTTCCGAGGCAGTTCTAGAGTCGATCGTTGGAAAGCACGGTGTTTCTCCTGCAGCAAAGCAAAGTTTGGCCACGAGGTTATCCGAATTGTTGAATGGGTGCAAGGAATCTAATGGTGTTGAAGCCGGAAATGAAAGGGCGGAGGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009812 GO:0009813 GO:0012505 GO:0016853 GO:0016872 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045430 GO:0050896 GO:0071704 GO:0080167 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00941 ko01100 ko01110 map00941 map01100 map01110

Pfam Domains

Protein Families

Chalcone

Protein Analysis

578

Amino Acids

62.39

Weight (kDa)

5.2

Isoelectric Point (pI)

36.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Chalcone	PF02431	15 - 72	1.7e-21	Chalcone-flavanone isomerase
Chalcone	PF02431	73 - 115	1.1e-13	Chalcone-flavanone isomerase
Chalcone	PF02431	125 - 327	3.6e-103	Chalcone-flavanone isomerase
Chalcone	PF02431	358 - 558	3.1e-99	Chalcone-flavanone isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004474)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55120
fragaria_vesca	FvH4_7g20870
malus_domestica	MD01G1117800.v1.1 MD01G1118000.v1.1 MD01G1118100.v1.1 MD01G1118300.v1.1 MD07G1186300.v1.1 MD07G1186400.v1.1
prunus_persica	Prupe.2G225200_v2.0.a1
pyrus_communis	pycom01g14550 pycom07g17740 pycom07g17750 pycom07g17760 pycom07g17770
rosa_chinensis	RchiOBHm_Chr1g0365111 RchiOBHm_Chr7g0221421 RchiOBHm_Chr7g0221431
rosa_laevigata	RLG00000027441
rosa_multiflora	Rmu_co8490591.1_g000001 Rmu_sc0001688.1_g000009 Rmu_sc0024062.1_g000001
rosa_roxburghii	Rroxscaffold_4G00291420 Rroxscaffold_4G00291930
rosa_rugosa	Rorug01G0319900
rosa_samantha	Rh1AG328600 Rh1BG290200 Rh1CG306900 Rh1DG321800 Rh7AG342300 Rh7CG359900
rosa_wichuraiana	Rw1G029160 Rw7G029060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	296, 785, 1478
AciI	CCGC	5 cut(s)	25, 438, 1137, 1418, 1724
AclWI	GGATC	3 cut(s)	337, 826, 1519
AcoI	YGGCCR	3 cut(s)	555, 960, 1653
AcsI	RAATTY	7 cut(s)	222, 283, 617, 711, 1310, 1404, 1465
AcuI	CTGAAG	6 cut(s)	184, 571, 879, 1052, 1264, 1572
AcyI	GRCGYC	2 cut(s)	786, 1479
AfaI	GTAC	5 cut(s)	135, 503, 855, 1196, 1548
AfiI	CCNNNNNNNGG	6 cut(s)	153, 540, 650, 1138, 1233, 1343
AhdI	GACNNNNNGTC	2 cut(s)	371, 1070
AjnI	CCWGG	1 cut(s)	369
AjuI	GAANNNNNNNTTGG	4 cut(s)	413, 445, 1112, 1144
AleI	CACNNNNGTG	1 cut(s)	59
AluBI	AGCT	8 cut(s)	529, 834, 863, 1016, 1043, 1222, 1527, 1556
AluI	AGCT	8 cut(s)	529, 834, 863, 1016, 1043, 1222, 1527, 1556
Alw21I	GWGCWC	1 cut(s)	56
Alw26I	GTCTC	1 cut(s)	371
Alw44I	GTGCAC	1 cut(s)	52
AlwI	GGATC	3 cut(s)	337, 826, 1519
AoxI	GGCC	6 cut(s)	246, 555, 652, 960, 1345, 1653
ApaLI	GTGCAC	1 cut(s)	52
ApeKI	GCWGC	4 cut(s)	863, 944, 1556, 1637
ApoI	RAATTY	7 cut(s)	222, 283, 617, 711, 1310, 1404, 1465
AspLEI	GCGC	3 cut(s)	443, 788, 1481
AspS9I	GGNCC	2 cut(s)	605, 1298
AsuC2I	CCSGG	5 cut(s)	294, 411, 783, 1110, 1476
AsuHPI	GGTGA	5 cut(s)	73, 460, 886, 1153, 1579
AvaII	GGWCC	2 cut(s)	605, 1298
BaeGI	GKGCMC	1 cut(s)	56
BalI	TGGCCA	1 cut(s)	1655
BanI	GGYRCC	3 cut(s)	296, 785, 1478
BanII	GRGCYC	2 cut(s)	416, 1115
BauI	CACGAG	1 cut(s)	1657
BbsI	GAAGAC	2 cut(s)	262, 751
Bbv12I	GWGCWC	1 cut(s)	56
BbvCI	CCTCAGC	2 cut(s)	525, 1218
BbvI	GCAGC	4 cut(s)	850, 956, 1543, 1649
BccI	CCATC	2 cut(s)	334, 361
BceAI	ACGGC	8 cut(s)	117, 131, 183, 504, 518, 570, 1197, 1211
BcgI	CGANNNNNNTGC	4 cut(s)	365, 399, 1064, 1098
BciT130I	CCWGG	1 cut(s)	371
BcnI	CCSGG	5 cut(s)	294, 411, 783, 1110, 1476
BcoDI	GTCTC	1 cut(s)	371
BfaI	CTAG	4 cut(s)	840, 908, 1533, 1601
BfmI	CTRYAG	2 cut(s)	942, 1635
BfoI	RGCGCY	2 cut(s)	789, 1482
BisI	GCNGC	5 cut(s)	439, 864, 945, 1557, 1638
BlsI	GCNGC	5 cut(s)	440, 865, 946, 1558, 1639
Bme1390I	CCNGG	6 cut(s)	294, 371, 411, 783, 1110, 1476
Bme18I	GGWCC	2 cut(s)	605, 1298
BmeRI	GACNNNNNGTC	2 cut(s)	371, 1070
BmgT120I	GGNCC	2 cut(s)	605, 1298
BmiI	GGNNCC	6 cut(s)	298, 345, 415, 787, 1114, 1480
BmrFI	CCNGG	6 cut(s)	294, 371, 411, 783, 1110, 1476
BmsI	GCATC	9 cut(s)	223, 712, 835, 857, 889, 997, 1405, 1528, 1550
BpiI	GAAGAC	2 cut(s)	262, 751
BpmI	CTGGAG	3 cut(s)	353, 479, 1172
Bpu10I	CCTNAGC	2 cut(s)	525, 1218
BpuEI	CTTGAG	3 cut(s)	284, 773, 1466
BpuMI	CCSGG	5 cut(s)	294, 411, 783, 1110, 1476
BsaHI	GRCGYC	2 cut(s)	786, 1479
BsaJI	CCNNGG	6 cut(s)	324, 433, 558, 1132, 1251, 1590
BsaXI	ACNNNNNCTCC	6 cut(s)	328, 358, 793, 823, 1486, 1516
Bsc4I	CCNNNNNNNGG	6 cut(s)	153, 540, 650, 1138, 1233, 1343
Bse3DI	GCAATG	1 cut(s)	1380
BseBI	CCWGG	1 cut(s)	371
BseDI	CCNNGG	6 cut(s)	324, 433, 558, 1132, 1251, 1590
BseGI	GGATG	3 cut(s)	848, 1420, 1541
BseLI	CCNNNNNNNGG	6 cut(s)	153, 540, 650, 1138, 1233, 1343
BseMI	GCAATG	1 cut(s)	1380
BseMII	CTCAG	3 cut(s)	539, 1232, 1350
BseRI	GAGGAG	3 cut(s)	188, 406, 575
BseSI	GKGCMC	1 cut(s)	56
BseX3I	CGGCCG	1 cut(s)	555
BseXI	GCAGC	4 cut(s)	850, 956, 1543, 1649
Bsh1285I	CGRYCG	5 cut(s)	360, 495, 558, 918, 1611
BshFI	GGCC	6 cut(s)	248, 557, 654, 962, 1347, 1655
BshNI	GGYRCC	3 cut(s)	296, 785, 1478
BsiEI	CGRYCG	5 cut(s)	360, 495, 558, 918, 1611
BsiHKAI	GWGCWC	1 cut(s)	56
BsiSI	CCGG	9 cut(s)	294, 363, 410, 783, 963, 1062, 1109, 1476, 1710
BslFI	GGGAC	3 cut(s)	218, 605, 1298
BslI	CCNNNNNNNGG	6 cut(s)	153, 540, 650, 1138, 1233, 1343
BsmAI	GTCTC	1 cut(s)	371
BsmBI	CGTCTC	1 cut(s)	371
BsmFI	GGGAC	3 cut(s)	218, 605, 1298
BsnI	GGCC	6 cut(s)	248, 557, 654, 962, 1347, 1655
Bsp1286I	GDGCHC	3 cut(s)	56, 416, 1115
Bsp143I	GATC	9 cut(s)	277, 329, 492, 766, 818, 915, 1459, 1511, 1608
BspACI	CCGC	5 cut(s)	25, 438, 1137, 1418, 1724
BspANI	GGCC	6 cut(s)	248, 557, 654, 962, 1347, 1655
BspCNI	CTCAG	3 cut(s)	538, 1231, 1351
BspLI	GGNNCC	6 cut(s)	298, 345, 415, 787, 1114, 1480
BspMAI	CTGCAG	2 cut(s)	946, 1639
BspPI	GGATC	3 cut(s)	337, 826, 1519
BspT107I	GGYRCC	3 cut(s)	296, 785, 1478
BsrDI	GCAATG	1 cut(s)	1380
BssECI	CCNNGG	6 cut(s)	324, 433, 558, 1132, 1251, 1590
BssMI	GATC	9 cut(s)	277, 329, 492, 766, 818, 915, 1459, 1511, 1608
BssNI	GRCGYC	2 cut(s)	786, 1479
BssSI	CACGAG	1 cut(s)	1657
BssT1I	CCWWGG	2 cut(s)	324, 1251
Bst2BI	CACGAG	1 cut(s)	1657
Bst2UI	CCWGG	1 cut(s)	371
Bst4CI	ACNGT	8 cut(s)	34, 217, 396, 874, 932, 1053, 1095, 1625
BstACI	GRCGYC	2 cut(s)	786, 1479
BstC8I	GCNNGC	7 cut(s)	13, 144, 531, 656, 1059, 1224, 1349
BstDEI	CTNAG	3 cut(s)	525, 1218, 1359
BstF5I	GGATG	3 cut(s)	848, 1420, 1541
BstH2I	RGCGCY	2 cut(s)	789, 1482
BstHHI	GCGC	3 cut(s)	443, 788, 1481
BstKTI	GATC	9 cut(s)	280, 332, 495, 769, 821, 918, 1462, 1514, 1611
BstMAI	GTCTC	1 cut(s)	371
BstMBI	GATC	9 cut(s)	277, 329, 492, 766, 818, 915, 1459, 1511, 1608
BstMCI	CGRYCG	5 cut(s)	360, 495, 558, 918, 1611
BstNI	CCWGG	1 cut(s)	371
BstSCI	CCNGG	6 cut(s)	292, 369, 409, 781, 1108, 1474
BstSFI	CTRYAG	2 cut(s)	942, 1635
BstSLI	GKGCMC	1 cut(s)	56
BstV1I	GCAGC	4 cut(s)	850, 956, 1543, 1649
BstV2I	GAAGAC	2 cut(s)	262, 751
BstZI	CGGCCG	1 cut(s)	555
BsuRI	GGCC	6 cut(s)	248, 557, 654, 962, 1347, 1655
BtsCI	GGATG	3 cut(s)	848, 1420, 1541
BtsI	GCAGTG	1 cut(s)	1218
BtsIMutI	CAGTG	6 cut(s)	228, 332, 644, 717, 1218, 1337
Cac8I	GCNNGC	7 cut(s)	13, 144, 531, 656, 1059, 1224, 1349
CfoI	GCGC	3 cut(s)	443, 788, 1481
Cfr13I	GGNCC	2 cut(s)	605, 1298
Csp6I	GTAC	5 cut(s)	134, 502, 854, 1195, 1547
CviAII	CATG	1 cut(s)	1731
CviQI	GTAC	5 cut(s)	134, 502, 854, 1195, 1547
DdeI	CTNAG	3 cut(s)	525, 1218, 1359
DinI	GGCGCC	2 cut(s)	787, 1480
DpnI	GATC	9 cut(s)	279, 331, 494, 768, 820, 917, 1461, 1513, 1610
DpnII	GATC	9 cut(s)	277, 329, 492, 766, 818, 915, 1459, 1511, 1608
DriI	GACNNNNNGTC	2 cut(s)	371, 1070
EaeI	YGGCCR	3 cut(s)	555, 960, 1653
EagI	CGGCCG	1 cut(s)	555
Eam1105I	GACNNNNNGTC	2 cut(s)	371, 1070
EclXI	CGGCCG	1 cut(s)	555
Eco130I	CCWWGG	2 cut(s)	324, 1251
Eco24I	GRGCYC	2 cut(s)	416, 1115
Eco47I	GGWCC	2 cut(s)	605, 1298
Eco52I	CGGCCG	1 cut(s)	555
Eco57I	CTGAAG	6 cut(s)	184, 571, 879, 1052, 1264, 1572
EcoRII	CCWGG	1 cut(s)	369
EcoT14I	CCWWGG	2 cut(s)	324, 1251
EcoT22I	ATGCAT	2 cut(s)	850, 1543
EcoT38I	GRGCYC	2 cut(s)	416, 1115
EgeI	GGCGCC	2 cut(s)	787, 1480
EheI	GGCGCC	2 cut(s)	787, 1480
ErhI	CCWWGG	2 cut(s)	324, 1251
Esp3I	CGTCTC	1 cut(s)	371
FaeI	CATG	1 cut(s)	1734
FaiI	YATR	2 cut(s)	115, 1732
FaqI	GGGAC	3 cut(s)	218, 605, 1298
FatI	CATG	1 cut(s)	1730
FauI	CCCGC	1 cut(s)	1130
Fnu4HI	GCNGC	5 cut(s)	439, 864, 945, 1557, 1638
FokI	GGATG	3 cut(s)	835, 1427, 1528
FriOI	GRGCYC	2 cut(s)	416, 1115
Fsp4HI	GCNGC	5 cut(s)	439, 864, 945, 1557, 1638
FspBI	CTAG	4 cut(s)	840, 908, 1533, 1601
GlaI	GCGC	3 cut(s)	442, 787, 1480
GluI	GCNGC	5 cut(s)	439, 864, 945, 1557, 1638
GsuI	CTGGAG	3 cut(s)	353, 479, 1172
HaeII	RGCGCY	2 cut(s)	789, 1482
HaeIII	GGCC	6 cut(s)	248, 557, 654, 962, 1347, 1655
HapII	CCGG	9 cut(s)	294, 363, 410, 783, 963, 1062, 1109, 1476, 1710
HhaI	GCGC	3 cut(s)	443, 788, 1481
Hin1I	GRCGYC	2 cut(s)	786, 1479
Hin1II	CATG	1 cut(s)	1734
Hin6I	GCGC	3 cut(s)	441, 786, 1479
HinP1I	GCGC	3 cut(s)	441, 786, 1479
HincII	GTYRAC	3 cut(s)	180, 567, 1260
HindII	GTYRAC	3 cut(s)	180, 567, 1260
HpaII	CCGG	9 cut(s)	294, 363, 410, 783, 963, 1062, 1109, 1476, 1710
HphI	GGTGA	5 cut(s)	73, 460, 886, 1153, 1579
Hpy166II	GTNNAC	9 cut(s)	54, 99, 180, 486, 502, 567, 1179, 1195, 1260
Hpy188III	TCNNGA	8 cut(s)	91, 376, 478, 840, 908, 1171, 1533, 1601
Hpy8I	GTNNAC	9 cut(s)	54, 99, 180, 486, 502, 567, 1179, 1195, 1260
Hpy99I	CGWCG	3 cut(s)	134, 521, 1057
HpyAV	CCTTC	6 cut(s)	151, 538, 722, 960, 1231, 1415
HpyCH4III	ACNGT	8 cut(s)	34, 217, 396, 874, 932, 1053, 1095, 1625
HpyCH4IV	ACGT	3 cut(s)	129, 516, 1563
HpyF3I	CTNAG	3 cut(s)	525, 1218, 1359
HpySE526I	ACGT	3 cut(s)	129, 516, 1563
Hsp92I	GRCGYC	2 cut(s)	786, 1479
Hsp92II	CATG	1 cut(s)	1734
HspAI	GCGC	3 cut(s)	441, 786, 1479
KasI	GGCGCC	2 cut(s)	785, 1478
Kzo9I	GATC	9 cut(s)	277, 329, 492, 766, 818, 915, 1459, 1511, 1608
LmnI	GCTCC	4 cut(s)	349, 419, 1048, 1118
Lsp1109I	GCAGC	4 cut(s)	850, 956, 1543, 1649
LweI	GCATC	9 cut(s)	223, 712, 835, 857, 889, 997, 1405, 1528, 1550
MaeI	CTAG	4 cut(s)	840, 908, 1533, 1601
MaeII	ACGT	3 cut(s)	129, 516, 1563
MaeIII	GTNAC	5 cut(s)	211, 598, 628, 1291, 1321
MalI	GATC	9 cut(s)	279, 331, 494, 768, 820, 917, 1461, 1513, 1610
MboI	GATC	9 cut(s)	277, 329, 492, 766, 818, 915, 1459, 1511, 1608
MboII	GAAGA	5 cut(s)	190, 262, 577, 751, 1270
MhlI	GDGCHC	3 cut(s)	56, 416, 1115
MlsI	TGGCCA	1 cut(s)	1655
MluNI	TGGCCA	1 cut(s)	1655
Mly113I	GGCGCC	2 cut(s)	786, 1479
MlyI	GAGTC	6 cut(s)	194, 360, 581, 920, 1274, 1613
MmeI	TCCRAC	7 cut(s)	51, 199, 371, 444, 901, 1381, 1594
Mox20I	TGGCCA	1 cut(s)	1655
Mph1103I	ATGCAT	2 cut(s)	850, 1543
MscI	TGGCCA	1 cut(s)	1655
MseI	TTAA	3 cut(s)	150, 537, 1230
MslI	CAYNNNNRTG	4 cut(s)	59, 626, 1319, 1436
Msp20I	TGGCCA	1 cut(s)	1655
MspI	CCGG	9 cut(s)	294, 363, 410, 783, 963, 1062, 1109, 1476, 1710
MspR9I	CCNGG	6 cut(s)	294, 371, 411, 783, 1110, 1476
MvaI	CCWGG	1 cut(s)	371
NarI	GGCGCC	2 cut(s)	786, 1479
NciI	CCSGG	5 cut(s)	294, 411, 783, 1110, 1476
NdeII	GATC	9 cut(s)	277, 329, 492, 766, 818, 915, 1459, 1511, 1608
NlaIII	CATG	1 cut(s)	1734
NlaIV	GGNNCC	6 cut(s)	298, 345, 415, 787, 1114, 1480
NmeAIII	GCCGAG	3 cut(s)	414, 583, 1113
NmuCI	GTSAC	2 cut(s)	628, 1321
NsiI	ATGCAT	2 cut(s)	850, 1543
OliI	CACNNNNGTG	1 cut(s)	59
PfeI	GAWTC	5 cut(s)	76, 463, 998, 1156, 1691
PflFI	GACNNNGTC	3 cut(s)	184, 571, 1264
PkrI	GCNGC	5 cut(s)	440, 865, 946, 1558, 1639
Ple19I	CGATCG	3 cut(s)	495, 918, 1611
PleI	GAGTC	6 cut(s)	193, 360, 580, 919, 1273, 1612
PluTI	GGCGCC	2 cut(s)	789, 1482
PpsI	GAGTC	6 cut(s)	193, 360, 580, 919, 1273, 1612
Psp6I	CCWGG	1 cut(s)	369
PspGI	CCWGG	1 cut(s)	369
PspN4I	GGNNCC	6 cut(s)	298, 345, 415, 787, 1114, 1480
PspPI	GGNCC	2 cut(s)	605, 1298
PstI	CTGCAG	2 cut(s)	946, 1639
PsyI	GACNNNGTC	3 cut(s)	184, 571, 1264
PvuI	CGATCG	3 cut(s)	495, 918, 1611
RsaI	GTAC	5 cut(s)	135, 503, 855, 1196, 1548
RsaNI	GTAC	5 cut(s)	134, 502, 854, 1195, 1547
RseI	CAYNNNNRTG	4 cut(s)	59, 626, 1319, 1436
SaqAI	TTAA	3 cut(s)	150, 537, 1230
SatI	GCNGC	5 cut(s)	439, 864, 945, 1557, 1638
Sau3AI	GATC	9 cut(s)	277, 329, 492, 766, 818, 915, 1459, 1511, 1608
Sau96I	GGNCC	2 cut(s)	605, 1298
SchI	GAGTC	6 cut(s)	194, 360, 581, 920, 1274, 1613
ScrFI	CCNGG	6 cut(s)	294, 371, 411, 783, 1110, 1476
SduI	GDGCHC	3 cut(s)	56, 416, 1115
SfaNI	GCATC	9 cut(s)	223, 712, 835, 857, 889, 997, 1405, 1528, 1550
SfcI	CTRYAG	2 cut(s)	942, 1635
SfoI	GGCGCC	2 cut(s)	787, 1480
SinI	GGWCC	2 cut(s)	605, 1298
SmiMI	CAYNNNNRTG	4 cut(s)	59, 626, 1319, 1436
SmlI	CTYRAG	3 cut(s)	263, 752, 1445
SmoI	CTYRAG	3 cut(s)	263, 752, 1445
SsiI	CCGC	5 cut(s)	25, 438, 1137, 1418, 1724
SspDI	GGCGCC	2 cut(s)	785, 1478
SspMI	CTAG	4 cut(s)	840, 908, 1533, 1601
StyD4I	CCNGG	6 cut(s)	292, 369, 409, 781, 1108, 1474
StyI	CCWWGG	2 cut(s)	324, 1251
TaaI	ACNGT	8 cut(s)	34, 217, 396, 874, 932, 1053, 1095, 1625
TaiI	ACGT	3 cut(s)	132, 519, 1566
TaqI	TCGA	4 cut(s)	375, 914, 1074, 1607
TaqII	GACCGA	1 cut(s)	346
TatI	WGTACW	2 cut(s)	501, 1194
TauI	GCSGC	1 cut(s)	441
TfiI	GAWTC	5 cut(s)	76, 463, 998, 1156, 1691
Tru1I	TTAA	3 cut(s)	150, 537, 1230
Tru9I	TTAA	3 cut(s)	150, 537, 1230
TscAI	CASTG	6 cut(s)	235, 339, 651, 724, 1218, 1344
TseFI	GTSAC	2 cut(s)	628, 1321
TseI	GCWGC	4 cut(s)	863, 944, 1556, 1637
Tsp45I	GTSAC	2 cut(s)	628, 1321
TspDTI	ATGAA	4 cut(s)	610, 1011, 1303, 1731
TspRI	CASTG	6 cut(s)	235, 339, 651, 724, 1218, 1344
Tth111I	GACNNNGTC	3 cut(s)	184, 571, 1264
VneI	GTGCAC	1 cut(s)	52
VpaK11BI	GGWCC	2 cut(s)	605, 1298
XapI	RAATTY	7 cut(s)	222, 283, 617, 711, 1310, 1404, 1465
XbaI	TCTAGA	4 cut(s)	839, 907, 1532, 1600
XspI	CTAG	4 cut(s)	840, 908, 1533, 1601
Zsp2I	ATGCAT	2 cut(s)	850, 1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.