pycom07g17770

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004474
Physical Location & Seq
Reverse (-)
20283216 .. 20283619
404 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17770.1

Sequence Viewer

Length: 285 bp
ATGACCTCACTACCCTCAGTCACCCCAGTTCAGGTTGAAACGACGACGTTTCTGTCGACCACCAAGCCTCCCGGCTCCGCCAACATCCTCTTCCTTGGCGGCACAGGGGTGAGGGGTCTGCCGATCGAGGGCAAGTTGGTGAAGTTCACAGTGATCGGGGTGTACTTAGAGGACAACGCCGTGCCTGTCCTTGCTGTCAAGTGGAAGGGCAAGACGGCCCAAGAGCTGGCGAACTCCGTCAAGTTCTTCAAGGACGTCGTCACAGGTAAATATATGACTAGCTAG

Protein Analysis

95

Amino Acids

10.02

Weight (kDa)

9.57

Isoelectric Point (pI)

31.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 12 - 91 1.2e-31 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 52
AatII GACGTC 1 cut(s) 258
AccB7I CCANNNNNTGG 1 cut(s) 226
AccI GTMKAC 1 cut(s) 56
AciI CCGC 2 cut(s) 78, 99
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 3 cut(s) 31, 128, 226
AgsI TTSAA 2 cut(s) 38, 250
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 2 cut(s) 226, 282
AluI AGCT 2 cut(s) 226, 282
AoxI GGCC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 3 cut(s) 13, 121, 151
BceAI ACGGC 2 cut(s) 164, 231
BcnI CCSGG 1 cut(s) 72
BfaI CTAG 2 cut(s) 279, 283
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 1 cut(s) 72
BmrI ACTGGG 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 128, 226
Bse1I ACTGG 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 94
BseGI GGATG 1 cut(s) 84
BseLI CCNNNNNNNGG 3 cut(s) 31, 128, 226
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 1 cut(s) 26
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 218
BsiEI CGRYCG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 72
BslI CCNNNNNNNGG 3 cut(s) 31, 128, 226
BsnI GGCC 1 cut(s) 218
Bsp143I GATC 2 cut(s) 123, 153
BspACI CCGC 2 cut(s) 78, 99
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 76
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 2 cut(s) 123, 153
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 94
Bst4CI ACNGT 1 cut(s) 151
Bst6I CTCTTC 1 cut(s) 95
BstACI GRCGYC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 2 cut(s) 16, 166
BstF5I GGATG 1 cut(s) 84
BstKTI GATC 2 cut(s) 126, 156
BstMBI GATC 2 cut(s) 123, 153
BstMCI CGRYCG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 70
BsuRI GGCC 1 cut(s) 218
BtsCI GGATG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 163
CviJI RGCY 5 cut(s) 67, 75, 218, 226, 282
CviKI_1 RGCY 5 cut(s) 67, 75, 218, 226, 282
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 2 cut(s) 16, 166
DpnI GATC 2 cut(s) 125, 155
DpnII GATC 2 cut(s) 123, 153
DrdI GACNNNNNNGTC 1 cut(s) 52
DseDI GACNNNNNNGTC 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
EciI GGCGGA 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 94
FaiI YATR 2 cut(s) 273, 275
FblI GTMKAC 1 cut(s) 56
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 1 cut(s) 100
FspBI CTAG 2 cut(s) 279, 283
GluI GCNGC 1 cut(s) 100
HaeIII GGCC 1 cut(s) 218
HapII CCGG 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 255
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HpaII CCGG 1 cut(s) 72
HphI GGTGA 3 cut(s) 13, 121, 151
Hpy166II GTNNAC 3 cut(s) 57, 147, 163
Hpy8I GTNNAC 3 cut(s) 57, 147, 163
Hpy99I CGWCG 3 cut(s) 46, 49, 260
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 2 cut(s) 47, 255
HpyF3I CTNAG 2 cut(s) 16, 166
HpySE526I ACGT 2 cut(s) 47, 255
Hsp92I GRCGYC 1 cut(s) 255
Kzo9I GATC 2 cut(s) 123, 153
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 7 cut(s) 17, 39, 85, 90, 198, 212, 249
MaeI CTAG 2 cut(s) 279, 283
MaeII ACGT 2 cut(s) 47, 255
MaeIII GTNAC 2 cut(s) 19, 259
MalI GATC 2 cut(s) 125, 155
MboI GATC 2 cut(s) 123, 153
MboII GAAGA 2 cut(s) 82, 238
MnlI CCTC 7 cut(s) 16, 25, 78, 98, 105, 121, 163
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 1 cut(s) 72
NciI CCSGG 1 cut(s) 72
NdeII GATC 2 cut(s) 123, 153
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 19, 259
OliI CACNNNNGTG 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PflFI GACNNNGTC 1 cut(s) 257
PflMI CCANNNNNTGG 1 cut(s) 226
PkrI GCNGC 1 cut(s) 101
Ple19I CGATCG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 1 cut(s) 217
PsyI GACNNNGTC 1 cut(s) 257
PvuI CGATCG 1 cut(s) 126
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 107
SalI GTCGAC 1 cut(s) 55
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 2 cut(s) 123, 153
Sau96I GGNCC 1 cut(s) 217
ScrFI CCNGG 1 cut(s) 72
SetI ASST 7 cut(s) 8, 36, 50, 228, 258, 268, 284
SmiMI CAYNNNNRTG 1 cut(s) 107
SsiI CCGC 2 cut(s) 78, 99
SspMI CTAG 2 cut(s) 279, 283
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 2 cut(s) 50, 258
TaqI TCGA 2 cut(s) 56, 126
TatI WGTACW 1 cut(s) 162
TauI GCSGC 1 cut(s) 102
TscAI CASTG 1 cut(s) 156
TseFI GTSAC 2 cut(s) 19, 259
Tsp45I GTSAC 2 cut(s) 19, 259
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 1 cut(s) 156
Tth111I GACNNNGTC 1 cut(s) 257
Van91I CCANNNNNTGG 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 56
XspI CTAG 2 cut(s) 279, 283
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.