RLG00000027441

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004474
Physical Location & Seq
Forward (+)
11704786 .. 11706122
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027441

Sequence Viewer

Length: 687 bp
ATGGCTCAATCAGTTACCGGAATTCAGGTAGAAGCCACCACGTTTCCTCCGGCCGTCAAGCCTCCGGGCTCCGCCAACACTCTGTTCCTCGCCGGCGCAGGGGCGAGGGGGCTGGAGATACAGGGGAATTTCGTTAAGTTCACGGCGATTGGAGTATACTTGGAGGATAAGGCCGTGCCGGCGCTCGCCGTTAAGTGGAAGGGTAAGACGGCCGAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGAGATCGTTACAGGTCCATTTGAGAAATTCACACAGGTGACGATGATACTACCATTGACAGGCCAGCAATACTCGGAGAAGGTTTCAGAGAATTGTGTTGCCATTTGGAAAAAATTTGGAATTTACACTGATGCAGAAGCCAAAGCTATTGAAAAGTTTGTGGAGGTCTTCAAAGATCAGACCTTCCCACCTGGTGCTTCTATTCTCTTCACACAATCACCAAATGGATCATTGACGATTGGCTTCTCCAAAGATGGTTCCATACCAGCAGTTGGAAATGCGGTGATTGAAAACAAGCTACTTTCAGAGTCAGTTCTCGAGTCAATCATTGGAAAGCAAGGTGTTTCTCCTGAAGCAAGGAAATGTGTGGCTACAAGGCTATCAGAATTGTTGAAAGAGAGTGATGATTGCGTGGCTGGAAATGGGAAAGTTGAAGAGTAA

Protein Analysis

229

Amino Acids

24.41

Weight (kDa)

5.08

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 10 - 212 2.5e-105 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 518
AccI GTMKAC 1 cut(s) 156
AciI CCGC 2 cut(s) 72, 527
AclWI GGATC 1 cut(s) 481
AcoI YGGCCR 2 cut(s) 51, 210
AcsI RAATTY 5 cut(s) 21, 127, 272, 359, 366
AcuI CTGAAG 2 cut(s) 226, 618
AdeI CACNNNGTG 1 cut(s) 440
AfiI CCNNNNNNNGG 4 cut(s) 99, 195, 305, 518
AgsI TTSAA 5 cut(s) 398, 418, 536, 640, 680
AjnI CCWGG 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 2 cut(s) 392, 544
AluI AGCT 2 cut(s) 392, 544
AlwI GGATC 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 4 cut(s) 51, 171, 210, 307
ApoI RAATTY 5 cut(s) 21, 127, 272, 359, 366
AspLEI GCGC 2 cut(s) 98, 184
AspS9I GGNCC 1 cut(s) 260
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 3 cut(s) 295, 456, 541
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 406
BccI CCATC 1 cut(s) 494
BceAI ACGGC 5 cut(s) 38, 158, 159, 173, 225
BciT130I CCWGG 1 cut(s) 438
BcnI CCSGG 1 cut(s) 66
BfoI RGCGCY 1 cut(s) 185
BglI GCCNNNNNGGC 1 cut(s) 179
Bme1390I CCNGG 2 cut(s) 66, 438
Bme18I GGWCC 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 2 cut(s) 70, 505
BmrFI CCNGG 2 cut(s) 66, 438
BmsI GCATC 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 195, 305, 518
Bse118I RCCGGY 2 cut(s) 92, 178
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 213
BseLI CCNNNNNNNGG 4 cut(s) 99, 195, 305, 518
BseRI GAGGAG 1 cut(s) 230
BseX3I CGGCCG 2 cut(s) 51, 210
Bsh1285I CGRYCG 2 cut(s) 54, 213
BshFI GGCC 4 cut(s) 53, 173, 212, 309
BsiEI CGRYCG 2 cut(s) 54, 213
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 5 cut(s) 18, 50, 65, 93, 179
BslI CCNNNNNNNGG 4 cut(s) 99, 195, 305, 518
BsnI GGCC 4 cut(s) 53, 173, 212, 309
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 3 cut(s) 249, 421, 473
BspACI CCGC 2 cut(s) 72, 527
BspANI GGCC 4 cut(s) 53, 173, 212, 309
BspLI GGNNCC 2 cut(s) 70, 505
BspPI GGATC 1 cut(s) 481
BsrFI RCCGGY 2 cut(s) 92, 178
BssAI RCCGGY 2 cut(s) 92, 178
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 3 cut(s) 249, 421, 473
BssNAI GTATAC 1 cut(s) 157
Bst1107I GTATAC 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 438
Bst6I CTCTTC 2 cut(s) 458, 675
BstC8I GCNNGC 4 cut(s) 94, 180, 186, 311
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 2 cut(s) 98, 184
BstKTI GATC 3 cut(s) 252, 424, 476
BstMBI GATC 3 cut(s) 249, 421, 473
BstMCI CGRYCG 2 cut(s) 54, 213
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 2 cut(s) 64, 436
BstV2I GAAGAC 1 cut(s) 406
BstZ17I GTATAC 1 cut(s) 157
BstZI CGGCCG 2 cut(s) 51, 210
BsuRI GGCC 4 cut(s) 53, 173, 212, 309
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 4 cut(s) 94, 180, 186, 311
CfoI GCGC 2 cut(s) 98, 184
Cfr10I RCCGGY 2 cut(s) 92, 178
Cfr13I GGNCC 1 cut(s) 260
CsiI ACCWGGT 1 cut(s) 436
DpnI GATC 3 cut(s) 251, 423, 475
DpnII GATC 3 cut(s) 249, 421, 473
DraIII CACNNNGTG 1 cut(s) 440
EaeI YGGCCR 2 cut(s) 51, 210
EagI CGGCCG 2 cut(s) 51, 210
Eam1104I CTCTTC 2 cut(s) 458, 675
EarI CTCTTC 2 cut(s) 458, 675
EciI GGCGGA 1 cut(s) 61
EclXI CGGCCG 2 cut(s) 51, 210
Eco24I GRGCYC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 260
Eco52I CGGCCG 2 cut(s) 51, 210
Eco57I CTGAAG 2 cut(s) 226, 618
Eco88I CYCGRG 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 71
FaiI YATR 2 cut(s) 157, 509
FblI GTMKAC 1 cut(s) 156
FriOI GRGCYC 1 cut(s) 71
GlaI GCGC 2 cut(s) 97, 183
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 4 cut(s) 53, 173, 212, 309
HapII CCGG 5 cut(s) 18, 50, 65, 93, 179
HhaI GCGC 2 cut(s) 98, 184
Hin6I GCGC 2 cut(s) 96, 182
HinP1I GCGC 2 cut(s) 96, 182
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 3 cut(s) 227, 554, 566
HpaII CCGG 5 cut(s) 18, 50, 65, 93, 179
HphI GGTGA 3 cut(s) 295, 456, 541
Hpy166II GTNNAC 3 cut(s) 141, 157, 222
Hpy188I TCNGA 5 cut(s) 322, 334, 426, 553, 631
Hpy188III TCNNGA 2 cut(s) 563, 596
Hpy8I GTNNAC 3 cut(s) 141, 157, 222
HpyAV CCTTC 3 cut(s) 193, 319, 439
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpySE526I ACGT 1 cut(s) 41
HspAI GCGC 2 cut(s) 96, 182
KroI GCCGGC 2 cut(s) 92, 178
KroNI GCCGGC 2 cut(s) 94, 180
Kzo9I GATC 3 cut(s) 249, 421, 473
LmnI GCTCC 1 cut(s) 74
LweI GCATC 1 cut(s) 367
MabI ACCWGGT 1 cut(s) 436
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 3 cut(s) 13, 253, 283
MalI GATC 3 cut(s) 251, 423, 475
MboI GATC 3 cut(s) 249, 421, 473
MboII GAAGA 3 cut(s) 232, 406, 445
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
MlyI GAGTC 3 cut(s) 236, 563, 575
MmeI TCCRAC 1 cut(s) 499
MnlI CCTC 7 cut(s) 57, 72, 98, 99, 157, 208, 403
MreI CGCCGGCG 1 cut(s) 92
MroNI GCCGGC 2 cut(s) 92, 178
MseI TTAA 2 cut(s) 135, 192
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 5 cut(s) 18, 50, 65, 93, 179
MspR9I CCNGG 2 cut(s) 66, 438
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 1 cut(s) 179
NaeI GCCGGC 2 cut(s) 94, 180
NciI CCSGG 1 cut(s) 66
NdeII GATC 3 cut(s) 249, 421, 473
NgoMIV GCCGGC 2 cut(s) 92, 178
NlaIV GGNNCC 2 cut(s) 70, 505
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 283
OliI CACNNNNGTG 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 563
PdiI GCCGGC 2 cut(s) 94, 180
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 518
PleI GAGTC 3 cut(s) 235, 562, 574
PpsI GAGTC 3 cut(s) 235, 562, 574
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 70, 505
PspPI GGNCC 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 2 cut(s) 135, 192
Sau3AI GATC 3 cut(s) 249, 421, 473
Sau96I GGNCC 1 cut(s) 260
SchI GAGTC 3 cut(s) 236, 563, 575
ScrFI CCNGG 2 cut(s) 66, 438
SduI GDGCHC 1 cut(s) 71
SexAI ACCWGGT 1 cut(s) 436
SfaNI GCATC 1 cut(s) 367
Sfr274I CTCGAG 1 cut(s) 563
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
SsiI CCGC 2 cut(s) 72, 527
StyD4I CCNGG 2 cut(s) 64, 436
TaiI ACGT 1 cut(s) 44
TaqI TCGA 1 cut(s) 564
TasI AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
Tru1I TTAA 2 cut(s) 135, 192
Tru9I TTAA 2 cut(s) 135, 192
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspGWI ACGGA 2 cut(s) 220, 239
TspRI CASTG 1 cut(s) 379
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 5 cut(s) 21, 127, 272, 359, 366
XhoI CTCGAG 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.