Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004474

Physical Location & Seq

Genomic Coordinates Reverse (-)

23197949 .. 23201355

Gene Structure 3407 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1118100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGGCTCCAACGCCATCGCTCGCCGGACTCCAGGTCGAGACGACTGCGTTTCCACCGTCCGTCAAACCTCCGGGCTCCCCCAACACTCTGTTCCTCGGCGGCGCAGGGGTGAGGGGGCTGGAGATTCAGGGGAACTTCGTGAAGTTCACGGCGATCGGAGTGTACTTGGAGGAAAACGCCGTGCCTCTGCTCGCCGTTAAGTGGAAGGGTAAGACGGCCGAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGACATTGTTACAGGTAACACGGATTTCTTCGCTGGCCAGCAATACTCTGACAAAGTTTCGGAGAATTGCGTTGCCTTTTGGAAGTCAATCGGAATTTACACTGATGCAGAAGGCAAAGCCATTGAAAAGTTCCTTGAGGTCTTCAAAGATCAAAACTTCCCACCCGGCGCCTCCATTCTTTTCACGCAATCTCCCAAAGGATCACTAACGATTAGCTTCTCTAGAGATGCATCCGTACCTGAAGCTGCAAACACGGTGATAGAAAACAAACTACTTTCCGAGGCAGTTCTAGAGTCGATCGTTGGAAAGCACGGTGTTTCCCCTGCAGCAAAGCAAAGTTTGGCCGAAAGGTTATCCAAATTGTTGAACGGGTGTAATGAATCTAAAGATGCCAAAGCCGGAAATGAGAAAGATTGTCAAATAGCTCCACCACCGTCGCTCGCCGGACTTCAGGTCGAAACGACTGCGTTTCCACCGTCCGTCAAACCTCCGGGCTCCTCCAACACTCTGTTCCTCGGCGGCGCAGGGGTGAGGGGGCTGGAGATTCAGGGGAACTTCGTGAAGTTCACGGCAATCGGAGTGTACTTGGAGGATAACGCCGTGCCTCAGCTCGCCGTTAAGTGGAAGGGTAAGACAGCCGAGGAGTTGATGGAGTCCGTTGAGTTCTTCAGGGACATCGTTACAGGTCCGTTTGAGAAATTCATTCAAGTGACAACGATATTGCCACTGACAGGCCAGCAATACTCTGACAAAGTTTCGGAGAATTGCGTTGTCTTTTGGAAGTCAGTCGGAATTTACACTGATGCAGAAGGCAAAGCCATTGAAAAGTTCCTTGAGGTCTTCAAAGATCAAAATTTCCCACCCGGTGCTTCCATTCTTTTCACACAATCTCCCAAAGGATCACTAACGATTAGCTTCTCTAGAAATGCATCCGTAGCTGAAGCTGCAAATGCGGTGATAGAAAACAAACTACTTTCCGAGGCAGTTCTAGAGTCGATCGTTGGAAAGCACGGTGTTTCTCCTGCAGCAAAGCAAAGTTTGGCCGCGAGGTTATCCGAATTGTTGAATGGGTGCAAGGAATCTAATGGTGCTGAAGCCGGAAATGAAAAAGTGGAGGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009812 GO:0009813 GO:0012505 GO:0016853 GO:0016872 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045430 GO:0050896 GO:0071704 GO:0080167 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00941 ko01100 ko01110 map00941 map01100 map01110

Pfam Domains

Protein Families

Chalcone

Protein Analysis

458

Amino Acids

48.71

Weight (kDa)

5.11

Isoelectric Point (pI)

34.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Chalcone	PF02431	12 - 205	9.1e-92	Chalcone-flavanone isomerase
Chalcone	PF02431	236 - 438	4.6e-104	Chalcone-flavanone isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004474)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55120
fragaria_vesca	FvH4_7g20870
malus_domestica	MD01G1117800.v1.1 MD01G1118000.v1.1 MD01G1118100.v1.1 MD01G1118300.v1.1 MD07G1186300.v1.1 MD07G1186400.v1.1
prunus_persica	Prupe.2G225200_v2.0.a1
pyrus_communis	pycom01g14550 pycom07g17740 pycom07g17750 pycom07g17760 pycom07g17770
rosa_chinensis	RchiOBHm_Chr1g0365111 RchiOBHm_Chr7g0221421 RchiOBHm_Chr7g0221431
rosa_laevigata	RLG00000027441
rosa_multiflora	Rmu_co8490591.1_g000001 Rmu_sc0001688.1_g000009 Rmu_sc0024062.1_g000001
rosa_roxburghii	Rroxscaffold_4G00291420 Rroxscaffold_4G00291930
rosa_rugosa	Rorug01G0319900
rosa_samantha	Rh1AG328600 Rh1BG290200 Rh1CG306900 Rh1DG321800 Rh7AG342300 Rh7CG359900
rosa_wichuraiana	Rw1G029160 Rw7G029060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	419
AccII	CGCG	1 cut(s)	1298
AciI	CCGC	4 cut(s)	99, 771, 1205, 1296
AclWI	GGATC	2 cut(s)	460, 1159
AcoI	YGGCCR	4 cut(s)	216, 286, 594, 1293
AcsI	RAATTY	4 cut(s)	345, 950, 1044, 1105
AcuI	CTGAAG	6 cut(s)	232, 513, 686, 904, 1212, 1365
AcyI	GRCGYC	1 cut(s)	420
AdeI	CACNNNGTG	1 cut(s)	1118
AfaI	GTAC	3 cut(s)	164, 489, 836
AfiI	CCNNNNNNNGG	3 cut(s)	201, 873, 983
AgsI	TTSAA	7 cut(s)	377, 397, 619, 959, 1076, 1096, 1318
AhdI	GACNNNNNGTC	2 cut(s)	32, 704
AjnI	CCWGG	1 cut(s)	30
AjuI	GAANNNNNNNTTGG	4 cut(s)	74, 106, 746, 778
AluBI	AGCT	7 cut(s)	468, 497, 677, 862, 1167, 1190, 1196
AluI	AGCT	7 cut(s)	468, 497, 677, 862, 1167, 1190, 1196
Alw26I	GTCTC	1 cut(s)	32
AlwI	GGATC	2 cut(s)	460, 1159
AoxI	GGCC	5 cut(s)	216, 286, 594, 985, 1293
ApeKI	GCWGC	4 cut(s)	497, 578, 1196, 1277
ApoI	RAATTY	4 cut(s)	345, 950, 1044, 1105
AspLEI	GCGC	3 cut(s)	104, 422, 776
AspS9I	GGNCC	1 cut(s)	938
AsuC2I	CCSGG	4 cut(s)	72, 417, 744, 1116
AsuHPI	GGTGA	4 cut(s)	121, 520, 793, 1219
AvaII	GGWCC	1 cut(s)	938
BalI	TGGCCA	1 cut(s)	288
BanI	GGYRCC	1 cut(s)	419
BanII	GRGCYC	2 cut(s)	77, 749
BbsI	GAAGAC	2 cut(s)	385, 1084
BbvCI	CCTCAGC	1 cut(s)	858
BbvI	GCAGC	4 cut(s)	484, 590, 1183, 1289
BccI	CCATC	2 cut(s)	22, 895
BceAI	ACGGC	7 cut(s)	164, 165, 179, 231, 836, 837, 851
BcgI	CGANNNNNNTGC	4 cut(s)	26, 60, 698, 732
BciT130I	CCWGG	1 cut(s)	32
BcnI	CCSGG	4 cut(s)	72, 417, 744, 1116
BcoDI	GTCTC	1 cut(s)	32
BfaI	CTAG	4 cut(s)	474, 542, 1173, 1241
BfmI	CTRYAG	2 cut(s)	576, 1275
BfoI	RGCGCY	1 cut(s)	423
BisI	GCNGC	7 cut(s)	100, 498, 579, 772, 1197, 1278, 1296
BlsI	GCNGC	7 cut(s)	101, 499, 580, 773, 1198, 1279, 1297
Bme1390I	CCNGG	5 cut(s)	32, 72, 417, 744, 1116
Bme18I	GGWCC	1 cut(s)	938
BmeRI	GACNNNNNGTC	2 cut(s)	32, 704
BmgT120I	GGNCC	1 cut(s)	938
BmiI	GGNNCC	4 cut(s)	6, 76, 421, 748
BmrFI	CCNGG	5 cut(s)	32, 72, 417, 744, 1116
BmsI	GCATC	6 cut(s)	346, 469, 491, 631, 1045, 1190
BpiI	GAAGAC	2 cut(s)	385, 1084
BpmI	CTGGAG	3 cut(s)	14, 140, 812
Bpu10I	CCTNAGC	1 cut(s)	858
BpuEI	CTTGAG	2 cut(s)	407, 1106
BpuMI	CCSGG	4 cut(s)	72, 417, 744, 1116
BsaHI	GRCGYC	1 cut(s)	420
BsaJI	CCNNGG	6 cut(s)	94, 219, 531, 766, 891, 1230
BsaXI	ACNNNNNCTCC	4 cut(s)	427, 457, 1126, 1156
Bsc4I	CCNNNNNNNGG	3 cut(s)	201, 873, 983
BseBI	CCWGG	1 cut(s)	32
BseDI	CCNNGG	6 cut(s)	94, 219, 531, 766, 891, 1230
BseGI	GGATG	2 cut(s)	482, 1181
BseLI	CCNNNNNNNGG	3 cut(s)	201, 873, 983
BseMII	CTCAG	1 cut(s)	872
BseRI	GAGGAG	3 cut(s)	236, 739, 908
BseX3I	CGGCCG	1 cut(s)	216
BseXI	GCAGC	4 cut(s)	484, 590, 1183, 1289
Bsh1236I	CGCG	1 cut(s)	1298
Bsh1285I	CGRYCG	4 cut(s)	156, 219, 552, 1251
BshFI	GGCC	5 cut(s)	218, 288, 596, 987, 1295
BshNI	GGYRCC	1 cut(s)	419
BsiEI	CGRYCG	4 cut(s)	156, 219, 552, 1251
BsiSI	CCGG	8 cut(s)	24, 71, 417, 651, 696, 743, 1116, 1350
BslFI	GGGAC	2 cut(s)	266, 938
BslI	CCNNNNNNNGG	3 cut(s)	201, 873, 983
BsmAI	GTCTC	1 cut(s)	32
BsmBI	CGTCTC	1 cut(s)	32
BsmFI	GGGAC	2 cut(s)	266, 938
BsnI	GGCC	5 cut(s)	218, 288, 596, 987, 1295
Bsp1286I	GDGCHC	2 cut(s)	77, 749
Bsp143I	GATC	7 cut(s)	153, 400, 452, 549, 1099, 1151, 1248
BspACI	CCGC	4 cut(s)	99, 771, 1205, 1296
BspANI	GGCC	5 cut(s)	218, 288, 596, 987, 1295
BspCNI	CTCAG	1 cut(s)	871
BspFNI	CGCG	1 cut(s)	1298
BspLI	GGNNCC	4 cut(s)	6, 76, 421, 748
BspMAI	CTGCAG	2 cut(s)	580, 1279
BspPI	GGATC	2 cut(s)	460, 1159
BspT107I	GGYRCC	1 cut(s)	419
BssECI	CCNNGG	6 cut(s)	94, 219, 531, 766, 891, 1230
BssMI	GATC	7 cut(s)	153, 400, 452, 549, 1099, 1151, 1248
BssNI	GRCGYC	1 cut(s)	420
Bst2UI	CCWGG	1 cut(s)	32
Bst4CI	ACNGT	6 cut(s)	57, 508, 566, 687, 729, 1265
BstACI	GRCGYC	1 cut(s)	420
BstC8I	GCNNGC	7 cut(s)	21, 192, 286, 290, 693, 864, 989
BstDEI	CTNAG	1 cut(s)	858
BstF5I	GGATG	2 cut(s)	482, 1181
BstFNI	CGCG	1 cut(s)	1298
BstH2I	RGCGCY	1 cut(s)	423
BstHHI	GCGC	3 cut(s)	104, 422, 776
BstKTI	GATC	7 cut(s)	156, 403, 455, 552, 1102, 1154, 1251
BstMAI	GTCTC	1 cut(s)	32
BstMBI	GATC	7 cut(s)	153, 400, 452, 549, 1099, 1151, 1248
BstMCI	CGRYCG	4 cut(s)	156, 219, 552, 1251
BstMWI	GCNNNNNNNGC	3 cut(s)	1187, 1196, 1202
BstNI	CCWGG	1 cut(s)	32
BstSCI	CCNGG	5 cut(s)	30, 70, 415, 742, 1114
BstSFI	CTRYAG	2 cut(s)	576, 1275
BstUI	CGCG	1 cut(s)	1298
BstV1I	GCAGC	4 cut(s)	484, 590, 1183, 1289
BstV2I	GAAGAC	2 cut(s)	385, 1084
BstZI	CGGCCG	1 cut(s)	216
BsuRI	GGCC	5 cut(s)	218, 288, 596, 987, 1295
BtsCI	GGATG	2 cut(s)	482, 1181
BtsIMutI	CAGTG	3 cut(s)	351, 977, 1050
Cac8I	GCNNGC	7 cut(s)	21, 192, 286, 290, 693, 864, 989
CfoI	GCGC	3 cut(s)	104, 422, 776
Cfr13I	GGNCC	1 cut(s)	938
Csp6I	GTAC	3 cut(s)	163, 488, 835
CviAII	CATG	1 cut(s)	1371
CviQI	GTAC	3 cut(s)	163, 488, 835
DdeI	CTNAG	1 cut(s)	858
DinI	GGCGCC	1 cut(s)	421
DpnI	GATC	7 cut(s)	155, 402, 454, 551, 1101, 1153, 1250
DpnII	GATC	7 cut(s)	153, 400, 452, 549, 1099, 1151, 1248
DraIII	CACNNNGTG	1 cut(s)	1118
DriI	GACNNNNNGTC	2 cut(s)	32, 704
EaeI	YGGCCR	4 cut(s)	216, 286, 594, 1293
EagI	CGGCCG	1 cut(s)	216
Eam1105I	GACNNNNNGTC	2 cut(s)	32, 704
EclXI	CGGCCG	1 cut(s)	216
Eco24I	GRGCYC	2 cut(s)	77, 749
Eco47I	GGWCC	1 cut(s)	938
Eco52I	CGGCCG	1 cut(s)	216
Eco57I	CTGAAG	6 cut(s)	232, 513, 686, 904, 1212, 1365
EcoRII	CCWGG	1 cut(s)	30
EcoT22I	ATGCAT	2 cut(s)	484, 1183
EcoT38I	GRGCYC	2 cut(s)	77, 749
EgeI	GGCGCC	1 cut(s)	421
EheI	GGCGCC	1 cut(s)	421
Esp3I	CGTCTC	1 cut(s)	32
FaeI	CATG	1 cut(s)	1374
FaiI	YATR	1 cut(s)	1372
FaqI	GGGAC	2 cut(s)	266, 938
FatI	CATG	1 cut(s)	1370
Fnu4HI	GCNGC	7 cut(s)	100, 498, 579, 772, 1197, 1278, 1296
FokI	GGATG	2 cut(s)	469, 1168
FriOI	GRGCYC	2 cut(s)	77, 749
Fsp4HI	GCNGC	7 cut(s)	100, 498, 579, 772, 1197, 1278, 1296
FspBI	CTAG	4 cut(s)	474, 542, 1173, 1241
GlaI	GCGC	3 cut(s)	103, 421, 775
GluI	GCNGC	7 cut(s)	100, 498, 579, 772, 1197, 1278, 1296
GsuI	CTGGAG	3 cut(s)	14, 140, 812
HaeII	RGCGCY	1 cut(s)	423
HaeIII	GGCC	5 cut(s)	218, 288, 596, 987, 1295
HapII	CCGG	8 cut(s)	24, 71, 417, 651, 696, 743, 1116, 1350
HhaI	GCGC	3 cut(s)	104, 422, 776
Hin1I	GRCGYC	1 cut(s)	420
Hin1II	CATG	1 cut(s)	1374
Hin6I	GCGC	3 cut(s)	102, 420, 774
HinP1I	GCGC	3 cut(s)	102, 420, 774
HincII	GTYRAC	1 cut(s)	228
HindII	GTYRAC	1 cut(s)	228
HinfI	GANTC	9 cut(s)	27, 124, 233, 545, 632, 796, 905, 1244, 1331
HpaII	CCGG	8 cut(s)	24, 71, 417, 651, 696, 743, 1116, 1350
HphI	GGTGA	4 cut(s)	121, 520, 793, 1219
Hpy166II	GTNNAC	5 cut(s)	147, 163, 228, 819, 835
Hpy188III	TCNNGA	7 cut(s)	37, 139, 474, 542, 811, 1173, 1241
Hpy8I	GTNNAC	5 cut(s)	147, 163, 228, 819, 835
Hpy99I	CGWCG	1 cut(s)	691
HpyAV	CCTTC	4 cut(s)	199, 356, 871, 1055
HpyCH4III	ACNGT	6 cut(s)	57, 508, 566, 687, 729, 1265
HpyCH4V	TGCA	9 cut(s)	359, 482, 500, 578, 1058, 1181, 1199, 1277, 1326
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1187, 1196, 1202
HpyF3I	CTNAG	1 cut(s)	858
Hsp92I	GRCGYC	1 cut(s)	420
Hsp92II	CATG	1 cut(s)	1374
HspAI	GCGC	3 cut(s)	102, 420, 774
KasI	GGCGCC	1 cut(s)	419
Kzo9I	GATC	7 cut(s)	153, 400, 452, 549, 1099, 1151, 1248
LmnI	GCTCC	4 cut(s)	10, 80, 682, 752
Lsp1109I	GCAGC	4 cut(s)	484, 590, 1183, 1289
LweI	GCATC	6 cut(s)	346, 469, 491, 631, 1045, 1190
MaeI	CTAG	4 cut(s)	474, 542, 1173, 1241
MaeIII	GTNAC	4 cut(s)	259, 266, 931, 961
MalI	GATC	7 cut(s)	155, 402, 454, 551, 1101, 1153, 1250
MboI	GATC	7 cut(s)	153, 400, 452, 549, 1099, 1151, 1248
MboII	GAAGA	5 cut(s)	238, 271, 385, 910, 1084
MhlI	GDGCHC	2 cut(s)	77, 749
MlsI	TGGCCA	1 cut(s)	288
MluCI	AATT	8 cut(s)	316, 345, 611, 950, 1015, 1044, 1105, 1310
MluNI	TGGCCA	1 cut(s)	288
Mly113I	GGCGCC	1 cut(s)	420
MlyI	GAGTC	5 cut(s)	21, 242, 554, 914, 1253
MmeI	TCCRAC	5 cut(s)	32, 535, 777, 1021, 1234
Mox20I	TGGCCA	1 cut(s)	288
Mph1103I	ATGCAT	2 cut(s)	484, 1183
MscI	TGGCCA	1 cut(s)	288
MseI	TTAA	2 cut(s)	198, 870
MslI	CAYNNNNRTG	1 cut(s)	959
Msp20I	TGGCCA	1 cut(s)	288
MspI	CCGG	8 cut(s)	24, 71, 417, 651, 696, 743, 1116, 1350
MspR9I	CCNGG	5 cut(s)	32, 72, 417, 744, 1116
MvaI	CCWGG	1 cut(s)	32
MvnI	CGCG	1 cut(s)	1298
MwoI	GCNNNNNNNGC	3 cut(s)	1187, 1196, 1202
NarI	GGCGCC	1 cut(s)	420
NciI	CCSGG	4 cut(s)	72, 417, 744, 1116
NdeII	GATC	7 cut(s)	153, 400, 452, 549, 1099, 1151, 1248
NlaIII	CATG	1 cut(s)	1374
NlaIV	GGNNCC	4 cut(s)	6, 76, 421, 748
NmeAIII	GCCGAG	4 cut(s)	75, 244, 747, 916
NmuCI	GTSAC	1 cut(s)	961
NsiI	ATGCAT	2 cut(s)	484, 1183
PfeI	GAWTC	4 cut(s)	124, 632, 796, 1331
PflFI	GACNNNGTC	1 cut(s)	232
PkrI	GCNGC	7 cut(s)	101, 499, 580, 773, 1198, 1279, 1297
Ple19I	CGATCG	3 cut(s)	156, 552, 1251
PleI	GAGTC	5 cut(s)	21, 241, 553, 913, 1252
PluTI	GGCGCC	1 cut(s)	423
PpsI	GAGTC	5 cut(s)	21, 241, 553, 913, 1252
Psp6I	CCWGG	1 cut(s)	30
PspGI	CCWGG	1 cut(s)	30
PspN4I	GGNNCC	4 cut(s)	6, 76, 421, 748
PspPI	GGNCC	1 cut(s)	938
PstI	CTGCAG	2 cut(s)	580, 1279
PsyI	GACNNNGTC	1 cut(s)	232
PvuI	CGATCG	3 cut(s)	156, 552, 1251
RsaI	GTAC	3 cut(s)	164, 489, 836
RsaNI	GTAC	3 cut(s)	163, 488, 835
RseI	CAYNNNNRTG	1 cut(s)	959
SaqAI	TTAA	2 cut(s)	198, 870
SatI	GCNGC	7 cut(s)	100, 498, 579, 772, 1197, 1278, 1296
Sau3AI	GATC	7 cut(s)	153, 400, 452, 549, 1099, 1151, 1248
Sau96I	GGNCC	1 cut(s)	938
SchI	GAGTC	5 cut(s)	21, 242, 554, 914, 1253
ScrFI	CCNGG	5 cut(s)	32, 72, 417, 744, 1116
SduI	GDGCHC	2 cut(s)	77, 749
SfaNI	GCATC	6 cut(s)	346, 469, 491, 631, 1045, 1190
SfcI	CTRYAG	2 cut(s)	576, 1275
SfoI	GGCGCC	1 cut(s)	421
SinI	GGWCC	1 cut(s)	938
SmiMI	CAYNNNNRTG	1 cut(s)	959
SmlI	CTYRAG	2 cut(s)	386, 1085
SmoI	CTYRAG	2 cut(s)	386, 1085
Sse9I	AATT	8 cut(s)	316, 345, 611, 950, 1015, 1044, 1105, 1310
SsiI	CCGC	4 cut(s)	99, 771, 1205, 1296
SspDI	GGCGCC	1 cut(s)	419
SspMI	CTAG	4 cut(s)	474, 542, 1173, 1241
StyD4I	CCNGG	5 cut(s)	30, 70, 415, 742, 1114
TaaI	ACNGT	6 cut(s)	57, 508, 566, 687, 729, 1265
TaqI	TCGA	4 cut(s)	36, 548, 708, 1247
TasI	AATT	8 cut(s)	316, 345, 611, 950, 1015, 1044, 1105, 1310
TatI	WGTACW	2 cut(s)	162, 834
TauI	GCSGC	3 cut(s)	102, 774, 1298
TfiI	GAWTC	4 cut(s)	124, 632, 796, 1331
Tru1I	TTAA	2 cut(s)	198, 870
Tru9I	TTAA	2 cut(s)	198, 870
TscAI	CASTG	3 cut(s)	358, 984, 1057
TseFI	GTSAC	1 cut(s)	961
TseI	GCWGC	4 cut(s)	497, 578, 1196, 1277
Tsp45I	GTSAC	1 cut(s)	961
TspDTI	ATGAA	3 cut(s)	645, 943, 1371
TspGWI	ACGGA	9 cut(s)	49, 226, 245, 287, 475, 721, 898, 930, 1174
TspRI	CASTG	3 cut(s)	358, 984, 1057
Tth111I	GACNNNGTC	1 cut(s)	232
VpaK11BI	GGWCC	1 cut(s)	938
XapI	RAATTY	4 cut(s)	345, 950, 1044, 1105
XbaI	TCTAGA	4 cut(s)	473, 541, 1172, 1240
XspI	CTAG	4 cut(s)	474, 542, 1173, 1241
Zsp2I	ATGCAT	2 cut(s)	484, 1183

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.