MD01G1118000.v1.1

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004474
Physical Location & Seq
Reverse (-)
23192892 .. 23194432
1541 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1118000.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGCTCCAACGCCATCGCTCGCCGGACTCCAGGTCGAGACGACTGCGTTTCCACCGTCCGTCAAACCTCCGGGCTCCTCCAACACTCTGTTCCTCGGAGGCGCAGGGGTGAGGGGGCTGGAGATTCAGGGGAACTTCGTGAAGTTCACGGCGATCGGAGTGTACTTGGAGGAAAACGCCGTGCCTCTGCTCGCCGTTAAGTGGAAGGGTAAGACGGCCGAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGACATCGTTACAGGTCCGTTTGAGAAATTCATTCAAGTGACAACGATACTGCCACTCACAGGCCAGCAATACTCTGACAAAGTTTCGGAGAATTGCGTTGCCTTTTGGAAGTCAATCGGAATTTACACTGATGCAGAAGGCAAAGCCATTGAAAAGTTCCTTGAGGTCTTCAAAGATCAAAACTTCCCACCCGGCGCCTCCATTCTTTTCACGCAATCTCCCAAAGGATCACTAACGATTAGCTTCTCTAGAGATGCATCTGTACCTGAAGCTGCAAACACGGTGATAGAAAACAAACTACTTTCCGAGGCAGTTCTAGAGTCGATCGTTGGAAAGCACGGTGTTTCTCCTGCAGCAAAGCAAAGTTTGGCCGCGAGGTTATCTGAATTGTTGAATGGGTGCAAGGAATCTTATCGTGCTGAAGCCGGAAATGAAAAAGTGGAGGCATGA

Protein Analysis

234

Amino Acids

24.96

Weight (kDa)

5.14

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 12 - 214 1.4e-105 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 446
AccII CGCG 1 cut(s) 626
AciI CCGC 1 cut(s) 624
AclWI GGATC 1 cut(s) 487
AcoI YGGCCR 2 cut(s) 216, 621
AcsI RAATTY 2 cut(s) 278, 372
AcuI CTGAAG 3 cut(s) 232, 540, 693
AcyI GRCGYC 1 cut(s) 447
AfaI GTAC 2 cut(s) 164, 516
AfiI CCNNNNNNNGG 2 cut(s) 201, 311
AgsI TTSAA 4 cut(s) 287, 404, 424, 646
AhdI GACNNNNNGTC 1 cut(s) 32
AjnI CCWGG 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 2 cut(s) 495, 524
AluI AGCT 2 cut(s) 495, 524
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 1 cut(s) 487
AoxI GGCC 3 cut(s) 216, 313, 621
ApeKI GCWGC 2 cut(s) 524, 605
ApoI RAATTY 2 cut(s) 278, 372
AspLEI GCGC 2 cut(s) 104, 449
AspS9I GGNCC 1 cut(s) 266
AsuC2I CCSGG 2 cut(s) 72, 444
AsuHPI GGTGA 2 cut(s) 121, 547
AvaII GGWCC 1 cut(s) 266
BanI GGYRCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 412
BbvI GCAGC 2 cut(s) 511, 617
BccI CCATC 1 cut(s) 22
BceAI ACGGC 4 cut(s) 164, 165, 179, 231
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 1 cut(s) 32
BcnI CCSGG 2 cut(s) 72, 444
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 2 cut(s) 501, 569
BfmI CTRYAG 1 cut(s) 603
BfoI RGCGCY 1 cut(s) 450
BisI GCNGC 3 cut(s) 525, 606, 624
BlsI GCNGC 3 cut(s) 526, 607, 625
Bme1390I CCNGG 3 cut(s) 32, 72, 444
Bme18I GGWCC 1 cut(s) 266
BmeRI GACNNNNNGTC 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 3 cut(s) 6, 76, 448
BmrFI CCNGG 3 cut(s) 32, 72, 444
BmsI GCATC 3 cut(s) 373, 496, 518
BpiI GAAGAC 1 cut(s) 412
BpmI CTGGAG 2 cut(s) 14, 140
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 2 cut(s) 72, 444
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 94, 219, 558
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 311
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 94, 219, 558
BseLI CCNNNNNNNGG 2 cut(s) 201, 311
BseRI GAGGAG 2 cut(s) 67, 236
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 511, 617
Bsh1236I CGCG 1 cut(s) 626
Bsh1285I CGRYCG 3 cut(s) 156, 219, 579
BshFI GGCC 3 cut(s) 218, 315, 623
BshNI GGYRCC 1 cut(s) 446
BsiEI CGRYCG 3 cut(s) 156, 219, 579
BsiSI CCGG 4 cut(s) 24, 71, 444, 678
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 201, 311
BsmAI GTCTC 1 cut(s) 32
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 3 cut(s) 218, 315, 623
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 4 cut(s) 153, 427, 479, 576
BspACI CCGC 1 cut(s) 624
BspANI GGCC 3 cut(s) 218, 315, 623
BspFNI CGCG 1 cut(s) 626
BspLI GGNNCC 3 cut(s) 6, 76, 448
BspMAI CTGCAG 1 cut(s) 607
BspPI GGATC 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 446
BssECI CCNNGG 3 cut(s) 94, 219, 558
BssMI GATC 4 cut(s) 153, 427, 479, 576
BssNI GRCGYC 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 3 cut(s) 57, 535, 593
BstACI GRCGYC 1 cut(s) 447
BstC8I GCNNGC 3 cut(s) 21, 192, 317
BstFNI CGCG 1 cut(s) 626
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 2 cut(s) 104, 449
BstKTI GATC 4 cut(s) 156, 430, 482, 579
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 4 cut(s) 153, 427, 479, 576
BstMCI CGRYCG 3 cut(s) 156, 219, 579
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 3 cut(s) 30, 70, 442
BstSFI CTRYAG 1 cut(s) 603
BstUI CGCG 1 cut(s) 626
BstV1I GCAGC 2 cut(s) 511, 617
BstV2I GAAGAC 1 cut(s) 412
BstZI CGGCCG 1 cut(s) 216
BsuRI GGCC 3 cut(s) 218, 315, 623
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 21, 192, 317
CfoI GCGC 2 cut(s) 104, 449
Cfr13I GGNCC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 163, 515
CviAII CATG 1 cut(s) 699
CviQI GTAC 2 cut(s) 163, 515
DinI GGCGCC 1 cut(s) 448
DpnI GATC 4 cut(s) 155, 429, 481, 578
DpnII GATC 4 cut(s) 153, 427, 479, 576
DriI GACNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 2 cut(s) 216, 621
EagI CGGCCG 1 cut(s) 216
Eam1105I GACNNNNNGTC 1 cut(s) 32
EclXI CGGCCG 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 77
Eco47I GGWCC 1 cut(s) 266
Eco52I CGGCCG 1 cut(s) 216
Eco57I CTGAAG 3 cut(s) 232, 540, 693
EcoRII CCWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 77
EgeI GGCGCC 1 cut(s) 448
EheI GGCGCC 1 cut(s) 448
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 1 cut(s) 702
FaiI YATR 1 cut(s) 700
FaqI GGGAC 1 cut(s) 266
FatI CATG 1 cut(s) 698
Fnu4HI GCNGC 3 cut(s) 525, 606, 624
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 3 cut(s) 525, 606, 624
FspBI CTAG 2 cut(s) 501, 569
GlaI GCGC 2 cut(s) 103, 448
GluI GCNGC 3 cut(s) 525, 606, 624
GsuI CTGGAG 2 cut(s) 14, 140
HaeII RGCGCY 1 cut(s) 450
HaeIII GGCC 3 cut(s) 218, 315, 623
HapII CCGG 4 cut(s) 24, 71, 444, 678
HhaI GCGC 2 cut(s) 104, 449
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 1 cut(s) 702
Hin6I GCGC 2 cut(s) 102, 447
HinP1I GCGC 2 cut(s) 102, 447
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 5 cut(s) 27, 124, 233, 572, 659
HpaII CCGG 4 cut(s) 24, 71, 444, 678
HphI GGTGA 2 cut(s) 121, 547
Hpy166II GTNNAC 3 cut(s) 147, 163, 228
Hpy188I TCNGA 7 cut(s) 98, 158, 328, 340, 371, 559, 637
Hpy188III TCNNGA 4 cut(s) 37, 139, 501, 569
Hpy8I GTNNAC 3 cut(s) 147, 163, 228
HpyAV CCTTC 2 cut(s) 199, 383
HpyCH4III ACNGT 3 cut(s) 57, 535, 593
HpyCH4V TGCA 5 cut(s) 386, 509, 527, 605, 654
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 1 cut(s) 702
HspAI GCGC 2 cut(s) 102, 447
KasI GGCGCC 1 cut(s) 446
Kzo9I GATC 4 cut(s) 153, 427, 479, 576
LmnI GCTCC 2 cut(s) 10, 80
Lsp1109I GCAGC 2 cut(s) 511, 617
LweI GCATC 3 cut(s) 373, 496, 518
MaeI CTAG 2 cut(s) 501, 569
MaeIII GTNAC 2 cut(s) 259, 289
MalI GATC 4 cut(s) 155, 429, 481, 578
MboI GATC 4 cut(s) 153, 427, 479, 576
MboII GAAGA 2 cut(s) 238, 412
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 4 cut(s) 278, 343, 372, 638
Mly113I GGCGCC 1 cut(s) 447
MlyI GAGTC 3 cut(s) 21, 242, 581
MmeI TCCRAC 3 cut(s) 32, 105, 562
Mph1103I ATGCAT 1 cut(s) 511
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 287
MspI CCGG 4 cut(s) 24, 71, 444, 678
MspR9I CCNGG 3 cut(s) 32, 72, 444
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 1 cut(s) 626
NarI GGCGCC 1 cut(s) 447
NciI CCSGG 2 cut(s) 72, 444
NdeII GATC 4 cut(s) 153, 427, 479, 576
NlaIII CATG 1 cut(s) 702
NlaIV GGNNCC 3 cut(s) 6, 76, 448
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 511
PfeI GAWTC 2 cut(s) 124, 659
PflFI GACNNNGTC 1 cut(s) 232
PkrI GCNGC 3 cut(s) 526, 607, 625
Ple19I CGATCG 2 cut(s) 156, 579
PleI GAGTC 3 cut(s) 21, 241, 580
PluTI GGCGCC 1 cut(s) 450
PpsI GAGTC 3 cut(s) 21, 241, 580
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 3 cut(s) 6, 76, 448
PspPI GGNCC 1 cut(s) 266
PstI CTGCAG 1 cut(s) 607
PsyI GACNNNGTC 1 cut(s) 232
PvuI CGATCG 2 cut(s) 156, 579
RsaI GTAC 2 cut(s) 164, 516
RsaNI GTAC 2 cut(s) 163, 515
RseI CAYNNNNRTG 1 cut(s) 287
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 3 cut(s) 525, 606, 624
Sau3AI GATC 4 cut(s) 153, 427, 479, 576
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 3 cut(s) 21, 242, 581
ScrFI CCNGG 3 cut(s) 32, 72, 444
SduI GDGCHC 1 cut(s) 77
SetI ASST 8 cut(s) 36, 70, 268, 420, 497, 520, 526, 632
SfaNI GCATC 3 cut(s) 373, 496, 518
SfcI CTRYAG 1 cut(s) 603
SfoI GGCGCC 1 cut(s) 448
SinI GGWCC 1 cut(s) 266
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 4 cut(s) 278, 343, 372, 638
SsiI CCGC 1 cut(s) 624
SspDI GGCGCC 1 cut(s) 446
SspMI CTAG 2 cut(s) 501, 569
StyD4I CCNGG 3 cut(s) 30, 70, 442
TaaI ACNGT 3 cut(s) 57, 535, 593
TaqI TCGA 2 cut(s) 36, 575
TasI AATT 4 cut(s) 278, 343, 372, 638
TatI WGTACW 1 cut(s) 162
TauI GCSGC 1 cut(s) 626
TfiI GAWTC 2 cut(s) 124, 659
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 2 cut(s) 524, 605
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 2 cut(s) 271, 699
TspGWI ACGGA 4 cut(s) 49, 226, 245, 258
TspRI CASTG 1 cut(s) 385
Tth111I GACNNNGTC 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 266
XapI RAATTY 2 cut(s) 278, 372
XbaI TCTAGA 2 cut(s) 500, 568
XspI CTAG 2 cut(s) 501, 569
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.